################################## # NO CUT ENZYMES ( 196 enzymes) # ################################## AatII Acc65I AccII AccIII AciI AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BalI BamHI BanII BbeI BbrPI BbvCI BbvI BcgI BciVI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmI BsoBI BspDI BspEI BspLU11I BspMI BsrBI BsrDI BsrFI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I Cfr13I ClaI CpoI DraI DraII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI Fnu4HI FseI FspI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII HphI ItaI KasI KpnI Ksp632I KspI MaeII MamI MfeI MflI MluI MluNI MroI MscI MspA1I MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RsrII SacI SacII SalI SapI Sau96I SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I StuI StyI SwaI TaqI TseI Tth111I TthHB8I XbaI XcmI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### AccI 457 GTAGAC GT^[AC][GT]AC AfaI 126 GTAC GT^AC AluI 130 AGCT AG^CT Asp700I 493 GAAAACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BanI 17 GGTGCC G^G[CT][GA]CC BbsI 172 GAAGAC GAAGAC(2/6) BclI 339 TGATCA T^GATCA BfaI 43 CTAG C^TAG BmyI 18 GTGCCC G[AGT]GC[ACT]^C BpmI 135 CTCCAG CTGGAG(16/14) BpuAI 172 GAAGAC GAAGAC(2/6) BsgI 296 CTGCAC GTGCAG(16/14) BsmFI 36 GGGAC GGGAC(10/14) Bsp1286I 18 GTGCCC G[AGT]GC[ACT]^C Bsp1407I 125 TGTACA T^GTACA BspHI 501 TCATGA T^CATGA BsrGI 125 TGTACA T^GTACA BsrI 137 CCAGT ACTGG(1/-1) BstAPI 424 GCAATAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Cac8I 433 GCCAGC GC[ACGT]^[ACGT]GC DraIII 94 CACTATGTG CAC[ACGT][ACGT][ACGT]^GTG FbaI 339 TGATCA T^GATCA HinfI 8 GAATC G^A[ACGT]TC MaeI 43 CTAG C^TAG MseI 350 TTAA T^TAA MslI 317 CACAGATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NlaIV 17 GGTGCC GG[ACGT]^[ACGT]CC NspI 420 ACATGC [GA]CATG^[CT] RcaI 501 TCATGA T^CATGA RsaI 126 GTAC GT^AC SspBI 125 TGTACA T^GTACA SspI 85 AATATT AAT^ATT TfiI 8 GAATC G^A[AT]TC Tru9I 350 TTAA T^TAA Tsp45I 140 GTGAC ^GT[GC]AC XmnI 493 GAAAACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 13 enzymes) # ################################### AlwI 508 GATCC GGATC(4/5) 531 GATCC BsaJI 149 CCAGGG C^C[ACGT][ACGT]GG 370 CCCAGG BstF5I 222 GGATG GGATG(2/0) 402 GGATG FokI 222 GGATG GGATG(9/13) 402 GGATG MaeIII 140 GTGAC ^GT[ACGT]AC 191 GTAAC MboII 172 GAAGA GAAGA(8/7) 80 TCTTC MwoI 360 GCGTAGAAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 424 GCAATAGATGC NlaIII 421 CATG CATG^ 502 CATG PflMI 93 CCACTATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 181 CCAATTTATGG SfaNI 276 GATGC GCATC(5/9) 430 GATGC Tsp509I 183 AATT ^AATT 229 AATT TspRI 74 CACTG CA[GC]TG[ACGT][ACGT]^ 138 CAGTG Van91I 93 CCACTATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 181 CCAATTTATGG ######################################### # 3 OR MORE CUT ENZYMES ( 13 enzymes) # ######################################### BsiYI BslI BssKI BstNI DdeI DpnII EcoRII MboI MnlI MvaI NdeII Sau3AI ScrFI