Restriction map for
pFN21AE1464
(Circular) (Six-base) MAPSORT of: pFN21AE1464.seq Check: 318 from: 1 to: 5263
pFN21AE1464 5263 bp
With 182 enzymes: SgfI *
August 16, 2010 14:48 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3073 278
Size: 53 83 186 936 1537 2468
Fragments arranged by size:
2468 1537 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2463 2606 1937
Size: 526 143 4594
Fragments arranged by size:
4594 526 143
Acc65I G'GTAC_C
Cuts at: 2584 2584
Size: 5263
AclI AA'CG_TT
Cuts at: 1563 2562 3390 3763 1563
Size: 999 828 373 3063
Fragments arranged by size:
3063 999 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3326 4710 4978 1632
Size: 1694 1384 268 1917
Fragments arranged by size:
1917 1694 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5066
AflIII A'CryG_T
Cuts at: 1203 4162 5139 1203
Size: 2959 977 1327
Fragments arranged by size:
2959 1327 977
AgeI A'CCGG_T
Cuts at: 4094 4094
Size: 5263
AhdI GACnn_n'nnGTC
Cuts at: 3992 3992
Size: 5263
AleI CACnn'nnGTG
Cuts at: 1166 2326 1166
Size: 1160 4103
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2442 1184
Size: 1258 4005
AlwNI CAG_nnn'CTG
Cuts at: 3006 4578 3006
Size: 1572 3691
ApaLI G'TGCA_C
Cuts at: 3320 4476 3320
Size: 1156 4107
ApoI r'AATT_y
Cuts at: 1493 2572 2798 2950 5059 1493
Size: 1079 226 152 2109 1697
Fragments arranged by size:
2109 1697 1079 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3817 160
Size: 872 2785 1606
Fragments arranged by size:
2785 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5263
AvaI C'yCGr_G
Cuts at: 1870 1943 2588 1870
Size: 73 645 4545
Fragments arranged by size:
4545 645 73
BamHI G'GATC_C
Cuts at: 2593 2960 2593
Size: 367 4896
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2022 2584 4039 618
Size: 325 217 627 235 562 1455 1842
Fragments arranged by size:
1842 1455 627 562 325 235 217
BanII G_rGCy'C
Cuts at: 1391 2582 1391
Size: 1191 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2184 928
Size: 971 285 4007
Fragments arranged by size:
4007 971 285
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3454 3488 1903
Size: 34 1517 34 3678
Fragments arranged by size:
3678 1517 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3155 4365 3155
Size: 1210 4053
BclI T'GATC_A
Cuts at: 1548 2344 1548
Size: 796 4467
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3874 136
Size: 107 122 71 631 605 2202 1525
Fragments arranged by size:
2202 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5258 5258
Size: 5263
BlpI GC'TnA_GC
Cuts at: 2680 2680
Size: 5263
Bme1580I G_kGCm'C
Cuts at: 1790 2027 3324 4480 1790
Size: 237 1297 1156 2573
Fragments arranged by size:
2573 1297 1156 237
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5263
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2994 3952 442
Size: 416 53 83 399 24 1577 958 1753
Fragments arranged by size:
1753 1577 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5263
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2125 3923 1239
Size: 886 1798 2579
Fragments arranged by size:
2579 1798 886
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3388 4253 4551 3388
Size: 865 298 4100
Fragments arranged by size:
4100 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3926 882
Size: 617 2427 2219
Fragments arranged by size:
2427 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3054 493
Size: 711 1850 2702
Fragments arranged by size:
2702 1850 711
BsaBI GATnn'nnATC
Cuts at: 5257 5257
Size: 5263
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3070 3452 4087 275
Size: 53 83 186 936 1537 382 635 1451
Fragments arranged by size:
1537 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3696 4094 4368 4515 4926 3696
Size: 398 274 147 411 4033
Fragments arranged by size:
4033 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2123 2153 2456 2486 1178
Size: 30 165 30 720 30 303 30 3955
Fragments arranged by size:
3955 720 303 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5263
BseYI C'CCAG_C
Cuts at: 4466 4962 4466
Size: 496 4767
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2286 1163
Size: 573 550 4140
Fragments arranged by size:
4140 573 550
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2713 3474 4502 1540
Size: 275 186 712 761 1028 2301
Fragments arranged by size:
2301 1028 761 712 275 186
BsiHKAI G_wGCw'C
Cuts at: 3324 3409 4480 3324
Size: 85 1071 4107
Fragments arranged by size:
4107 1071 85
BsmI GAATG_Cn'
Cuts at: 2774 2867 2774
Size: 93 5170
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2027 2582 3324 3409 4480 1391
Size: 399 237 555 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 555 399 237 85
BspEI T'CCGG_A
Cuts at: 4926 4926
Size: 5263
BspHI T'CATG_A
Cuts at: 2506 3152 4882 2506
Size: 646 1730 2887
Fragments arranged by size:
2887 1730 646
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2618 844
Size: 1774 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3150 1394
Size: 429 1327 3507
Fragments arranged by size:
3507 1327 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3758 3932 65
Size: 1183 2510 174 1396
Fragments arranged by size:
2510 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3907 4094 1700
Size: 110 2097 187 2869
Fragments arranged by size:
2869 2097 187 110
BsrGI T'GTAC_A
Cuts at: 96 2130 96
Size: 2034 3229
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5263
BssSI C'ACGA_G
Cuts at: 3323 4335 3323
Size: 1012 4251
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2435 794
Size: 1641 3622
BstBI TT'CG_AA
Cuts at: 4067 4067
Size: 5263
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5263
BstYI r'GATC_y
Cuts at: 1972 2593 2960 3345 3362 4814 4900 4912 5258
Size: 621 367 385 17 1452 86 12 346
Cuts at: 5258 1972
Size: 1977
Fragments arranged by size:
1977 1452 621 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4711
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4524
Fragments arranged by size:
4524 683 56
BtsI GCAGTG_nn'
Cuts at: 2779 3573 3593 2779
Size: 794 20 4449
Fragments arranged by size:
4449 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5263
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5228
DraI TTT'AAA
Cuts at: 2567 2567
Size: 5263
DraIII CAC_nnn'GTG
Cuts at: 2104 2104
Size: 5263
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4270 809
Size: 507 2954 1802
Fragments arranged by size:
2954 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2710 3599 5228
Size: 54 1425 50 275 18 880 889 1629
Cuts at: 5228 8
Size: 43
Fragments arranged by size:
1629 1425 889 880 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2710 1537
Size: 275 898 4090
Fragments arranged by size:
4090 898 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3193 1353
Size: 1840 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3850 4224 4370 387
Size: 1064 138 2261 374 146 1280
Fragments arranged by size:
2261 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2125 3326 3923 4710 4978 1239
Size: 393 493 1201 597 787 268 1524
Fragments arranged by size:
1524 1201 787 597 493 393 268
EcoRI G'AATT_C
Cuts at: 2572 2950 2572
Size: 378 4885
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2473 2473
Size: 5263
FspI TGC'GCA
Cuts at: 3769 3769
Size: 5263
HaeII r_GCGC'y
Cuts at: 1678 4410 1678
Size: 2732 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2456 2488 3904 3936 3978
Size: 32 135 32 884 32 1416 32 42
Cuts at: 3978 4010 1373
Size: 32 2626
Fragments arranged by size:
2626 1416 884 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2607 2853 669
Size: 988 281 669 246 3079
Fragments arranged by size:
3079 988 669 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5263
HpaI GTT'AAC
Cuts at: 2853 2853
Size: 5263
KpnI G_GTAC'C
Cuts at: 2588 2588
Size: 5263
MfeI C'AATT_G
Cuts at: 2862 2862
Size: 5263
MluI A'CGCG_T
Cuts at: 5139 5139
Size: 5263
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4377 4561 1305
Size: 3072 184 2007
Fragments arranged by size:
3072 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3441
Fragments arranged by size:
3441 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2326 3221 3580 3739 518
Size: 648 1160 895 359 159 2042
Fragments arranged by size:
2042 1160 895 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2679 3356 4504 4749 1606
Size: 1073 677 1148 245 2120
Fragments arranged by size:
2120 1148 1073 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4711
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5263
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5263
NotI GC'GGCC_GC
Cuts at: 2710 2710
Size: 5263
NspI r_CATG'y
Cuts at: 1132 1229 2429 2621 4166 1132
Size: 97 1200 192 1545 2229
Fragments arranged by size:
2229 1545 1200 192 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5263
PciI A'CATG_T
Cuts at: 4162 4162
Size: 5263
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2104 2192 1108
Size: 996 88 4179
Fragments arranged by size:
4179 996 88
PmeI GTTT'AAAC
Cuts at: 2567 2567
Size: 5263
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3332 3364 4845 4877 3332
Size: 32 1481 32 3718
Fragments arranged by size:
3718 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5263
PsiI TTA'TAA
Cuts at: 2833 2833
Size: 5263
PstI C_TGCA'G
Cuts at: 830 2615 830
Size: 1785 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5263
SalI G'TCGA_C
Cuts at: 1936 2605 1936
Size: 669 4594
SbfI CC_TGCA'GG
Cuts at: 2615 2615
Size: 5263
ScaI AGT'ACT
Cuts at: 3511 5223 3511
Size: 1712 3551
SfcI C'TryA_G
Cuts at: 826 1045 2611 3746 4427 4618 826
Size: 219 1566 1135 681 191 1471
Fragments arranged by size:
1566 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5263
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5263
SmaI CCC'GGG
Cuts at: 1872 2590 1872
Size: 718 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3367 4268 4530 820
Size: 197 926 1424 901 262 1553
Fragments arranged by size:
1553 1424 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3054 493
Size: 2561 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5263
SphI G_CATG'C
Cuts at: 1132 2621 1132
Size: 1489 3774
SspI AAT'ATT
Cuts at: 5 52 2092 3187 5
Size: 47 2040 1095 2081
Fragments arranged by size:
2081 2040 1095 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5263
StyI C'CwwG_G
Cuts at: 513 1065 2702 513
Size: 552 1637 3074
Fragments arranged by size:
3074 1637 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2010 3290 3307 3460 3645 4090 325
Size: 1255 430 1280 17 153 185 445 1498
Fragments arranged by size:
1498 1280 1255 445 430 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2130 3509 5221
Size: 274 80 33 51 1443 153 1379 1712
Cuts at: 5221 96
Size: 138
Fragments arranged by size:
1712 1443 1379 274 153 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5223 5255 5223
Size: 32 5231
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5263
XbaI T'CTAG_A
Cuts at: 2599 2599
Size: 5263
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5263
XmaI C'CCGG_G
Cuts at: 1870 2588 1870
Size: 718 4545
XmnI GAAnn'nnTTC
Cuts at: 2502 3392 2502
Size: 890 4373
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3071 276
Size: 53 83 186 936 1537 2468
Fragments arranged by size:
2468 1537 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI