################################## # NO CUT ENZYMES ( 124 enzymes) # ################################## Acc65I AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AviII AvrII BalI BbrPI BbsI BbvCI BcgI BciVI BclI BfaI BfrI BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BseAI BseRI BsiEI BsiWI BsmI BspDI BspEI BspHI BspLU11I BsrBI BssSI Bst1107I BstBI BstEII BstPI BstZ17I CelII ClaI CpoI DraI DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KpnI KspI MaeI MamI MfeI MluI MluNI MroI MscI MunI NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI ScaI SexAI SfaNI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI SwaI TaqI TthHB8I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AatII 288 GACGTC GACGT^C AccI 499 GTCTAC GT^[AC][GT]AC AlwNI 201 CAGCGACTG CAG[ACGT][ACGT][ACGT]^CTG AocI 558 CCTCAGG CC^T[ACGT]AGG AspI 847 GACGCGGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 314 CCCGAG C^[CT]CG[GA]G AvaII 321 GGACC G^G[AT]CC BamHI 485 GGATCC G^GATCC BglI 751 GCCCTGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 455 AGATCT A^GATCT BsaI 163 GAGACC GGTCTC(1/5) BsgI 391 CTGCAC GTGCAG(16/14) BsmBI 286 GAGACG CGTCTC(1/5) BsoBI 314 CCCGAG C^[CT]CG[GA]G BsrDI 732 CATTGC GCAATG(2/0) BssHII 96 GCGCGC G^CGCGC BstAPI 200 GCAGCGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Bsu36I 558 CCTCAGG CC^T[ACGT]AGG DraIII 371 CACTGCGTG CAC[ACGT][ACGT][ACGT]^GTG Eco81I 558 CCTCAGG CC^T[ACGT]AGG EcoNI 554 CCTGCCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FauI 18 GCGGG CCCGC(4/6) HinfI 699 GAATC G^A[ACGT]TC HphI 524 TCACC GGTGA(8/7) MaeII 289 ACGT A^CGT MseI 944 TTAA T^TAA NaeI 924 GCCGGC GCC^GGC NcoI 47 CCATGG C^CATGG NdeI 337 CATATG CA^TATG NgoMIV 924 GCCGGC G^CCGGC PflFI 847 GACGCGGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 238 CCACTGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG SacI 544 GAGCTC GAGCT^C SapI 546 GCTCTTC GCTCTTC(1/4) SbfI 753 CCTGCAGG CCTGCA^GG SfiI 750 GGCCCTGCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC Sse8387I 753 CCTGCAGG CCTGCA^GG TfiI 699 GAATC G^A[AT]TC Tru9I 944 TTAA T^TAA Tsp509I 219 AATT ^AATT Tth111I 847 GACGCGGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 238 CCACTGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AlwI 486 GATCC GGATC(4/5) 485 GGATC ApaI 20 GGGCCC GGGCC^C 913 GGGCCC AspHI 544 GAGCTC G[AT]GC[AT]^C 715 GAGCAC BsaWI 272 TCCGGT [AT]^CCGG[AT] 803 TCCGGT BsiHKAI 544 GAGCTC G[AT]GC[AT]^C 715 GAGCAC BspMI 332 ACCTGC ACCTGC(4/8) 531 GCAGGT BsrFI 616 ACCGGC [GA]^CCGG[CT] 924 GCCGGC BsrI 707 ACTGG ACTGG(1/-1) 156 CCAGT BstXI 32 CCACGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 47 CCATGGAGATGG BstYI 455 AGATCT [GA]^GATC[CT] 485 GGATCC Cfr10I 616 ACCGGC [GA]^CCGG[CT] 924 GCCGGC DdeI 559 CTCAG C^T[ACGT]AG 574 CTGAG DpnII 456 GATC ^GATC 486 GATC MaeIII 91 GTCAC ^GT[ACGT]AC 291 GTCAC MboI 456 GATC ^GATC 486 GATC MflI 455 AGATCT [GA]^GATC[CT] 485 GGATCC MslI 48 CATGGAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 354 CACCCCGGTG NdeII 456 GATC ^GATC 486 GATC NspI 509 GCATGT [GA]CATG^[CT] 863 GCATGT Sau3AI 456 GATC ^GATC 486 GATC Tsp45I 91 GTCAC ^GT[GC]AC 291 GTCAC XhoII 455 AGATCT [GA]^GATC[CT] 485 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 70 enzymes) # ######################################### AccII AciI AcyI AfaI AluI BanI BanII BbeI BbvI BcnI BmyI BpmI BsaHI BsaJI BsiYI BslI BsmAI BsmFI Bsp1286I Bsp1407I BsrGI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HpaII ItaI KasI Ksp632I MboII MnlI MspA1I MspI MvaI MvnI MwoI NarI NciI NlaIII NlaIV PstI PvuII RsaI Sau96I ScrFI SfcI SfoI SspBI StyI TseI TspRI