(Circular) (Six-base) MAPSORT of: pFN21AE6348.seq Check: 2236 from: 1 to: 5698 pFN21AE6348 5698 bp With 182 enzymes: SgfI * March 18, 2015 14:51 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2527 2527 Size: 5698 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2297 3508 278 Size: 53 83 186 936 761 1211 2468 Fragments arranged by size: 2468 1211 936 761 186 83 53 AccI GT'mk_AC Cuts at: 1937 2505 3041 1937 Size: 568 536 4594 Fragments arranged by size: 4594 568 536 Acc65I G'GTAC_C Cuts at: 3019 3019 Size: 5698 AclI AA'CG_TT Cuts at: 1563 3825 4198 1563 Size: 2262 373 3063 Fragments arranged by size: 3063 2262 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2282 2321 3761 5145 5413 1632 Size: 650 39 1440 1384 268 1917 Fragments arranged by size: 1917 1440 1384 650 268 39 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5501 AflIII A'CryG_T Cuts at: 1203 4597 5574 1203 Size: 3394 977 1327 Fragments arranged by size: 3394 1327 977 AgeI A'CCGG_T Cuts at: 4529 4529 Size: 5698 AhdI GACnn_n'nnGTC Cuts at: 4427 4427 Size: 5698 AleI CACnn'nnGTG Cuts at: 1166 2363 1166 Size: 1197 4501 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2985 1184 Size: 1801 3897 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2396 2428 2396 Size: 32 5666 AlwNI CAG_nnn'CTG Cuts at: 2211 3441 5013 2211 Size: 1230 1572 2896 Fragments arranged by size: 2896 1572 1230 ApaI G_GGCC'C Cuts at: 2029 2922 2029 Size: 893 4805 ApaLI G'TGCA_C Cuts at: 3755 4911 3755 Size: 1156 4542 ApoI r'AATT_y Cuts at: 1493 3007 3233 3385 5494 1493 Size: 1514 226 152 2109 1697 Fragments arranged by size: 2109 1697 1514 226 152 AseI AT'TA_AT Cuts at: 160 1032 4252 160 Size: 872 3220 1606 Fragments arranged by size: 3220 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5698 AvaI C'yCGr_G Cuts at: 1870 1943 2319 3023 1870 Size: 73 376 704 4545 Fragments arranged by size: 4545 704 376 73 BamHI G'GATC_C Cuts at: 2490 3028 3395 2490 Size: 538 367 4793 Fragments arranged by size: 4793 538 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2048 2064 2774 2840 3019 Size: 325 217 627 261 16 710 66 179 Cuts at: 3019 4474 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 710 627 325 261 217 179 66 16 BanII G_rGCy'C Cuts at: 1391 2029 2193 2553 2922 3017 1391 Size: 638 164 360 369 95 4072 Fragments arranged by size: 4072 638 369 360 164 95 BbeI G_GCGC'C Cuts at: 2052 2068 2778 2052 Size: 16 710 4972 Fragments arranged by size: 4972 710 16 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4727 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3889 3923 1903 Size: 34 1952 34 3678 Fragments arranged by size: 3678 1952 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3590 4800 3590 Size: 1210 4488 BclI T'GATC_A Cuts at: 1548 1548 Size: 5698 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2762 4309 136 Size: 107 122 71 631 605 1090 1547 1525 Fragments arranged by size: 1547 1525 1090 631 605 122 107 71 BglII A'GATC_T Cuts at: 2460 5693 2460 Size: 3233 2465 BlpI GC'TnA_GC Cuts at: 3115 3115 Size: 5698 Bme1580I G_kGCm'C Cuts at: 1790 2029 2845 2922 3759 4915 1790 Size: 239 816 77 837 1156 2573 Fragments arranged by size: 2573 1156 837 816 239 77 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5698 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3429 4387 442 Size: 416 53 83 399 24 2012 958 1753 Fragments arranged by size: 2012 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5698 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2345 2345 Size: 5698 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2132 2309 2900 2909 2945 4358 1239 Size: 893 177 591 9 36 1413 2579 Fragments arranged by size: 2579 1413 893 591 177 36 9 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3823 4688 4986 3823 Size: 865 298 4535 Fragments arranged by size: 4535 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2162 4361 882 Size: 617 663 2199 2219 Fragments arranged by size: 2219 2199 663 617 BsaAI yAC'GTr Cuts at: 493 1204 3489 493 Size: 711 2285 2702 Fragments arranged by size: 2702 2285 711 BsaBI GATnn'nnATC Cuts at: 5692 5692 Size: 5698 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2049 2065 2294 2775 Size: 53 83 186 936 516 16 229 481 Cuts at: 2775 2943 3505 3887 4522 275 Size: 168 562 382 635 1451 Fragments arranged by size: 1451 936 635 562 516 481 382 229 186 168 83 53 16 BsaWI w'CCGG_w Cuts at: 2277 2808 4131 4529 4803 4950 5361 2277 Size: 531 1323 398 274 147 411 2614 Fragments arranged by size: 2614 1323 531 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2396 2426 1178 Size: 30 165 30 993 30 4450 Fragments arranged by size: 4450 993 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5698 BseYI C'CCAG_C Cuts at: 2044 2197 2606 2786 4901 5397 2044 Size: 153 409 180 2115 496 2345 Fragments arranged by size: 2345 2115 496 409 180 153 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2381 1163 Size: 573 645 4480 Fragments arranged by size: 4480 645 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3148 3909 4937 1540 Size: 275 186 1147 761 1028 2301 Fragments arranged by size: 2301 1147 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2553 2724 3759 3844 4915 2553 Size: 171 1035 85 1071 3336 Fragments arranged by size: 3336 1071 1035 171 85 BsmI GAATG_Cn' Cuts at: 3209 3302 3209 Size: 93 5605 BsmBI CGTCTCn'nnnn_ Cuts at: 2285 2285 Size: 5698 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2029 2193 2553 2724 2845 2922 3017 Size: 399 239 164 360 171 121 77 95 Cuts at: 3017 3759 3844 4915 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 360 239 171 164 121 95 85 77 BspEI T'CCGG_A Cuts at: 5361 5361 Size: 5698 BspHI T'CATG_A Cuts at: 3587 5317 3587 Size: 1730 3968 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2346 2527 3053 844 Size: 1502 181 526 3489 Fragments arranged by size: 3489 1502 526 181 BsrBI CCG'CTC Cuts at: 1394 1823 3585 1394 Size: 429 1762 3507 Fragments arranged by size: 3507 1762 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2736 4193 4367 65 Size: 1183 1488 1457 174 1396 Fragments arranged by size: 1488 1457 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2621 2929 4342 4529 1700 Size: 110 811 308 1413 187 2869 Fragments arranged by size: 2869 1413 811 308 187 110 BsrGI T'GTAC_A Cuts at: 96 2148 2517 2697 96 Size: 2052 369 180 3097 Fragments arranged by size: 3097 2052 369 180 BssHII G'CGCG_C Cuts at: 1925 2101 1925 Size: 176 5522 BssSI C'ACGA_G Cuts at: 3758 4770 3758 Size: 1012 4686 BstAPI GCAn_nnn'nTGC Cuts at: 794 2211 794 Size: 1417 4281 BstBI TT'CG_AA Cuts at: 4502 4502 Size: 5698 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5698 BstXI CCAn_nnnn'nTGG Cuts at: 2044 2059 2044 Size: 15 5683 BstYI r'GATC_y Cuts at: 1972 2460 2490 3028 3395 3780 3797 5249 5335 Size: 488 30 538 367 385 17 1452 86 Cuts at: 5335 5347 5693 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 538 488 385 367 346 86 30 17 12 Bsu36I CC'TnA_GG Cuts at: 2564 2564 Size: 5698 BtgI C'CryG_G Cuts at: 513 1065 2037 2052 2661 2845 513 Size: 552 972 15 609 184 3366 Fragments arranged by size: 3366 972 609 552 184 15 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4959 Fragments arranged by size: 4959 683 56 BtsI GCAGTG_nn' Cuts at: 2318 2375 3214 4008 4028 2318 Size: 57 839 794 20 3988 Fragments arranged by size: 3988 839 794 57 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5698 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5663 DraI TTT'AAA Cuts at: 3002 3002 Size: 5698 DraIII CAC_nnn'GTG Cuts at: 2381 2381 Size: 5698 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4705 809 Size: 507 3389 1802 Fragments arranged by size: 3389 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2030 2119 2454 Size: 54 1425 50 275 18 200 89 335 Cuts at: 2454 2799 3145 4034 5663 8 Size: 345 346 889 1629 43 Fragments arranged by size: 1629 1425 889 346 345 335 275 200 89 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3145 1537 Size: 275 1333 4090 Fragments arranged by size: 4090 1333 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2336 2364 2558 3628 1353 Size: 983 28 194 1070 3423 Fragments arranged by size: 3423 1070 983 194 28 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4285 4659 4805 387 Size: 1064 138 2696 374 146 1280 Fragments arranged by size: 2696 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2551 2551 Size: 5698 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2132 2282 2309 2321 2900 2909 2945 Size: 393 500 150 27 12 579 9 36 Cuts at: 2945 3761 4358 5145 5413 1239 Size: 816 597 787 268 1524 Fragments arranged by size: 1524 816 787 597 579 500 393 268 150 36 27 12 9 EcoNI CCTnn'n_nnAGG Cuts at: 2563 2563 Size: 5698 EcoO109I rG'GnC_Cy Cuts at: 2611 2755 2918 2919 2611 Size: 144 163 1 5390 Fragments arranged by size: 5390 163 144 1 EcoRI G'AATT_C Cuts at: 3007 3385 3007 Size: 378 5320 FspI TGC'GCA Cuts at: 4204 4204 Size: 5698 HaeII r_GCGC'y Cuts at: 1678 2052 2068 2091 2778 2784 4845 1678 Size: 374 16 23 687 6 2061 2531 Fragments arranged by size: 2531 2061 687 374 23 16 6 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2147 2179 4339 4371 4413 Size: 32 135 32 575 32 2160 32 42 Cuts at: 4413 4445 1373 Size: 32 2626 Fragments arranged by size: 2626 2160 575 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3042 3288 669 Size: 988 281 1104 246 3079 Fragments arranged by size: 3079 1104 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5698 HpaI GTT'AAC Cuts at: 3288 3288 Size: 5698 KasI G'GCGC_C Cuts at: 2048 2064 2774 2048 Size: 16 710 4972 Fragments arranged by size: 4972 710 16 KpnI G_GTAC'C Cuts at: 3023 3023 Size: 5698 MfeI C'AATT_G Cuts at: 3297 3297 Size: 5698 MluI A'CGCG_T Cuts at: 5574 5574 Size: 5698 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2457 4812 4996 1305 Size: 1152 2355 184 2007 Fragments arranged by size: 2355 2007 1152 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3876 Fragments arranged by size: 3876 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2057 2363 3656 4015 4174 518 Size: 648 891 306 1293 359 159 2042 Fragments arranged by size: 2042 1293 891 648 359 306 159 MspA1I CmG'CkG Cuts at: 1606 2017 2287 2395 2833 2887 2906 3114 3791 Size: 411 270 108 438 54 19 208 677 Cuts at: 3791 4939 5184 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 438 411 270 245 208 108 54 19 NaeI GCC'GGC Cuts at: 2931 2931 Size: 5698 NarI GG'CG_CC Cuts at: 2049 2065 2775 2049 Size: 16 710 4972 Fragments arranged by size: 4972 710 16 NcoI C'CATG_G Cuts at: 513 1065 2052 513 Size: 552 987 4159 Fragments arranged by size: 4159 987 552 NdeI CA'TA_TG Cuts at: 387 2343 387 Size: 1956 3742 NgoMIV G'CCGG_C Cuts at: 2929 2929 Size: 5698 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5698 NotI GC'GGCC_GC Cuts at: 3145 3145 Size: 5698 NspI r_CATG'y Cuts at: 1132 1229 2518 2872 3056 4601 1132 Size: 97 1289 354 184 1545 2229 Fragments arranged by size: 2229 1545 1289 354 184 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5698 PciI A'CATG_T Cuts at: 4597 4597 Size: 5698 PflMI CCAn_nnn'nTGG Cuts at: 1108 2249 1108 Size: 1141 4557 PfoI T'CCnGG_A Cuts at: 2109 2109 Size: 5698 PmeI GTTT'AAAC Cuts at: 3002 3002 Size: 5698 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2396 2428 3767 3799 5280 5312 2396 Size: 32 1339 32 1481 32 2782 Fragments arranged by size: 2782 1481 1339 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5698 PsiI TTA'TAA Cuts at: 3268 3268 Size: 5698 PspOMI G'GGCC_C Cuts at: 2025 2918 2025 Size: 893 4805 PstI C_TGCA'G Cuts at: 830 2216 2313 2763 2838 3050 830 Size: 1386 97 450 75 212 3478 Fragments arranged by size: 3478 1386 450 212 97 75 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5698 PvuII CAG'CTG Cuts at: 2017 2287 2395 2833 2887 2017 Size: 270 108 438 54 4828 Fragments arranged by size: 4828 438 270 108 54 SacI G_AGCT'C Cuts at: 2553 2553 Size: 5698 SalI G'TCGA_C Cuts at: 1936 3040 1936 Size: 1104 4594 SapI GCTCTTCn'nnn_ Cuts at: 2558 2558 Size: 5698 SbfI CC_TGCA'GG Cuts at: 2763 3050 2763 Size: 287 5411 ScaI AGT'ACT Cuts at: 3946 5658 3946 Size: 1712 3986 SfcI C'TryA_G Cuts at: 826 1045 2212 2309 2759 2834 3046 4181 4862 Size: 219 1167 97 450 75 212 1135 681 Cuts at: 4862 5053 826 Size: 191 1471 Fragments arranged by size: 1471 1167 1135 681 450 219 212 191 97 75 SfiI GGCCn_nnn'nGGCC Cuts at: 2762 2762 Size: 5698 SfoI GGC'GCC Cuts at: 2050 2066 2776 2050 Size: 16 710 4972 Fragments arranged by size: 4972 710 16 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5698 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5698 SmaI CCC'GGG Cuts at: 1872 3025 1872 Size: 1153 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3802 4703 4965 820 Size: 197 926 1859 901 262 1553 Fragments arranged by size: 1859 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3489 493 Size: 2996 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5698 SphI G_CATG'C Cuts at: 1132 3056 1132 Size: 1924 3774 SspI AAT'ATT Cuts at: 5 52 3622 5 Size: 47 3570 2081 Fragments arranged by size: 3570 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5698 StyI C'CwwG_G Cuts at: 513 1065 2052 2176 2232 3137 513 Size: 552 987 124 56 905 3074 Fragments arranged by size: 3074 987 905 552 124 56 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3725 3742 3895 4080 4525 325 Size: 1255 2145 17 153 185 445 1498 Fragments arranged by size: 2145 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2148 2517 2628 Size: 274 80 33 51 1443 171 369 111 Cuts at: 2628 2697 3944 5656 96 Size: 69 1247 1712 138 Fragments arranged by size: 1712 1443 1247 369 274 171 138 111 80 69 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5658 5690 5658 Size: 32 5666 Tth111I GACn'n_nGTC Cuts at: 1316 2855 1316 Size: 1539 4159 XbaI T'CTAG_A Cuts at: 3034 3034 Size: 5698 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5698 XmaI C'CCGG_G Cuts at: 1870 3023 1870 Size: 1153 4545 XmnI GAAnn'nnTTC Cuts at: 3827 3827 Size: 5698 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2295 3506 276 Size: 53 83 186 936 761 1211 2468 Fragments arranged by size: 2468 1211 936 761 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BstZ17I EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacII SanDI SexAI SrfI SwaI XcmI