(Linear) (Six-base) MAPSORT of: KIEE6348.seq Check: 6302 from: 1 to: 993 KIEE6348.orf With 182 enzymes: SgfI * March 16, 2015 11:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 522 993 Size: 522 471 AatII G_ACGT'C Cuts at: 0 292 993 Size: 292 701 AccI GT'mk_AC Cuts at: 0 500 993 Size: 500 493 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 277 316 993 Size: 277 39 677 Fragments arranged by size: 677 277 39 AleI CACnn'nnGTG Cuts at: 0 358 993 Size: 358 635 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 980 993 Size: 980 13 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 391 423 993 Size: 391 32 570 Fragments arranged by size: 570 391 32 AlwNI CAG_nnn'CTG Cuts at: 0 206 993 Size: 206 787 ApaI G_GGCC'C Cuts at: 0 24 917 993 Size: 24 893 76 Fragments arranged by size: 893 76 24 AvaI C'yCGr_G Cuts at: 0 314 993 Size: 314 679 BamHI G'GATC_C Cuts at: 0 485 993 Size: 485 508 BanI G'GyrC_C Cuts at: 0 43 59 769 835 993 Size: 43 16 710 66 158 Fragments arranged by size: 710 158 66 43 16 BanII G_rGCy'C Cuts at: 0 24 188 548 917 993 Size: 24 164 360 369 76 Fragments arranged by size: 369 360 164 76 24 BbeI G_GCGC'C Cuts at: 0 47 63 773 993 Size: 47 16 710 220 Fragments arranged by size: 710 220 47 16 BglI GCCn_nnn'nGGC Cuts at: 0 757 993 Size: 757 236 BglII A'GATC_T Cuts at: 0 455 993 Size: 455 538 Bme1580I G_kGCm'C Cuts at: 0 24 840 917 993 Size: 24 816 77 76 Fragments arranged by size: 816 77 76 24 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 340 993 Size: 340 653 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 127 304 895 904 940 993 Size: 127 177 591 9 36 53 Fragments arranged by size: 591 177 127 53 36 9 BsaI GGTCTCn'nnnn_ Cuts at: 0 157 993 Size: 157 836 BsaHI Gr'CG_yC Cuts at: 0 44 60 289 770 938 993 Size: 44 16 229 481 168 55 Fragments arranged by size: 481 229 168 55 44 16 BsaWI w'CCGG_w Cuts at: 0 272 803 993 Size: 272 531 190 Fragments arranged by size: 531 272 190 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 391 421 993 Size: 391 30 572 Fragments arranged by size: 572 391 30 BseYI C'CCAG_C Cuts at: 0 39 192 601 781 993 Size: 39 153 409 180 212 Fragments arranged by size: 409 212 180 153 39 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 376 993 Size: 376 617 BsiHKAI G_wGCw'C Cuts at: 0 548 719 993 Size: 548 171 274 Fragments arranged by size: 548 274 171 BsmBI CGTCTCn'nnnn_ Cuts at: 0 280 993 Size: 280 713 Bsp1286I G_dGCh'C Cuts at: 0 24 188 548 719 840 917 993 Size: 24 164 360 171 121 77 76 Fragments arranged by size: 360 171 164 121 77 76 24 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 341 522 993 Size: 341 181 471 Fragments arranged by size: 471 341 181 BsrDI GCAATG_nn' Cuts at: 0 731 993 Size: 731 262 BsrFI r'CCGG_y Cuts at: 0 616 924 993 Size: 616 308 69 Fragments arranged by size: 616 308 69 BsrGI T'GTAC_A Cuts at: 0 143 512 692 993 Size: 143 369 180 301 Fragments arranged by size: 369 301 180 143 BssHII G'CGCG_C Cuts at: 0 96 993 Size: 96 897 BstAPI GCAn_nnn'nTGC Cuts at: 0 206 993 Size: 206 787 BstXI CCAn_nnnn'nTGG Cuts at: 0 39 54 993 Size: 39 15 939 Fragments arranged by size: 939 39 15 BstYI r'GATC_y Cuts at: 0 455 485 993 Size: 455 30 508 Fragments arranged by size: 508 455 30 Bsu36I CC'TnA_GG Cuts at: 0 559 993 Size: 559 434 BtgI C'CryG_G Cuts at: 0 32 47 656 840 993 Size: 32 15 609 184 153 Fragments arranged by size: 609 184 153 32 15 BtsI GCAGTG_nn' Cuts at: 0 313 370 993 Size: 313 57 623 Fragments arranged by size: 623 313 57 DraIII CAC_nnn'GTG Cuts at: 0 376 993 Size: 376 617 EaeI y'GGCC_r Cuts at: 0 25 114 449 794 993 Size: 25 89 335 345 199 Fragments arranged by size: 345 335 199 89 25 EarI CTCTTCn'nnn_ Cuts at: 0 331 359 553 993 Size: 331 28 194 440 Fragments arranged by size: 440 331 194 28 EcoICRI GAG'CTC Cuts at: 0 546 993 Size: 546 447 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 127 277 304 316 895 904 940 993 Size: 127 150 27 12 579 9 36 53 Fragments arranged by size: 579 150 127 53 36 27 12 9 EcoNI CCTnn'n_nnAGG Cuts at: 0 558 993 Size: 558 435 EcoO109I rG'GnC_Cy Cuts at: 0 606 750 913 914 993 Size: 606 144 163 1 79 Fragments arranged by size: 606 163 144 79 1 HaeII r_GCGC'y Cuts at: 0 47 63 86 773 779 993 Size: 47 16 23 687 6 214 Fragments arranged by size: 687 214 47 23 16 6 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 142 174 993 Size: 142 32 819 Fragments arranged by size: 819 142 32 KasI G'GCGC_C Cuts at: 0 43 59 769 993 Size: 43 16 710 224 Fragments arranged by size: 710 224 43 16 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 452 993 Size: 452 541 MslI CAynn'nnrTG Cuts at: 0 52 358 993 Size: 52 306 635 Fragments arranged by size: 635 306 52 MspA1I CmG'CkG Cuts at: 0 12 282 390 828 882 901 993 Size: 12 270 108 438 54 19 92 Fragments arranged by size: 438 270 108 92 54 19 12 NaeI GCC'GGC Cuts at: 0 926 993 Size: 926 67 NarI GG'CG_CC Cuts at: 0 44 60 770 993 Size: 44 16 710 223 Fragments arranged by size: 710 223 44 16 NcoI C'CATG_G Cuts at: 0 47 993 Size: 47 946 NdeI CA'TA_TG Cuts at: 0 338 993 Size: 338 655 NgoMIV G'CCGG_C Cuts at: 0 924 993 Size: 924 69 NspI r_CATG'y Cuts at: 0 513 867 993 Size: 513 354 126 Fragments arranged by size: 513 354 126 PflMI CCAn_nnn'nTGG Cuts at: 0 244 993 Size: 244 749 PfoI T'CCnGG_A Cuts at: 0 104 993 Size: 104 889 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 391 423 993 Size: 391 32 570 Fragments arranged by size: 570 391 32 PspOMI G'GGCC_C Cuts at: 0 20 913 993 Size: 20 893 80 Fragments arranged by size: 893 80 20 PstI C_TGCA'G Cuts at: 0 211 308 758 833 993 Size: 211 97 450 75 160 Fragments arranged by size: 450 211 160 97 75 PvuII CAG'CTG Cuts at: 0 12 282 390 828 882 993 Size: 12 270 108 438 54 111 Fragments arranged by size: 438 270 111 108 54 12 SacI G_AGCT'C Cuts at: 0 548 993 Size: 548 445 SapI GCTCTTCn'nnn_ Cuts at: 0 553 993 Size: 553 440 SbfI CC_TGCA'GG Cuts at: 0 758 993 Size: 758 235 SfcI C'TryA_G Cuts at: 0 207 304 754 829 993 Size: 207 97 450 75 164 Fragments arranged by size: 450 207 164 97 75 SfiI GGCCn_nnn'nGGCC Cuts at: 0 757 993 Size: 757 236 SfoI GGC'GCC Cuts at: 0 45 61 771 993 Size: 45 16 710 222 Fragments arranged by size: 710 222 45 16 StyI C'CwwG_G Cuts at: 0 47 171 227 993 Size: 47 124 56 766 Fragments arranged by size: 766 124 56 47 TatI w'GTAC_w Cuts at: 0 143 512 623 692 993 Size: 143 369 111 69 301 Fragments arranged by size: 369 301 143 111 69 Tth111I GACn'n_nGTC Cuts at: 0 850 993 Size: 850 143 ZraI GAC'GTC Cuts at: 0 290 993 Size: 290 703 Enzymes that do cut: AarI AatII AccI AcuI AleI AlfI AloI AlwNI ApaI AvaI BamHI BanI BanII BbeI BglI BglII Bme1580I BplI BpmI BsaI BsaHI BsaWI BsaXI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspMI BsrDI BsrFI BsrGI BssHII BstAPI BstXI BstYI Bsu36I BtgI BtsI DraIII EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I KasI MmeI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NspI PflMI PfoI PpiI PspOMI PstI PvuII SacI SapI SbfI SfcI SfiI SfoI StyI TatI Tth111I ZraI Enzymes that do not cut: Acc65I AclI AfeI AflII AflIII AgeI AhdI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BbsI BbvCI BcgI BciVI BclI BfrBI BlpI BmgBI BmrI BmtI Bpu10I BpuEI BsaAI BsaBI BseRI BsiEI BsiWI BsmI BspEI BspHI BsrBI BssSI BstBI BstEII BstZ17I BtgZI ClaI CspCI DraI DrdI EagI EciI EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KpnI MfeI MluI MscI NheI NotI NruI NsiI PacI PasI PciI PmeI PmlI PpuMI PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI ScaI SexAI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII TstI XbaI XcmI XhoI XmaI XmnI