################################## # NO CUT ENZYMES ( 119 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI Asp718I AspEI AspI AvaI AviII AvrII BamHI BanI BanII BbeI BbrPI BbvCI BcgI BclI BfrI BglII BlnI BsaAI BsaHI BsaWI BseAI BsiEI BsiWI BsmBI BsoBI BspDI BspEI BspLU11I BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI Bsu36I Cfr10I ClaI CpoI DraII DraIII DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO109I EcoRV EcoT22I FbaI FseI FspI HgaI Hin1I HindIII KasI KpnI KspI MaeII MluI MroI NaeI NarI NcoI NgoMIV NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PvuI RsrII SacI SacII SalI SapI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspI SwaI Tth111I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AccI 1608 GTATAC GT^[AC][GT]AC AlwNI 851 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG BalI 35 TGGCCA TGG^CCA BciVI 892 GGATAC GTATCC(6/5) BcnI 1131 CCGGG CC^[GC]GG BglI 649 GCCCAGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 932 GCTCAGC GC^T[ACGT]AGC Bpu1102I 932 GCTCAGC GC^T[ACGT]AGC BsaBI 1029 GATGCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsmI 503 GAATGC GAATGC(1/-1) BspHI 1185 TCATGA T^CATGA BssHII 43 GCGCGC G^CGCGC Bst1107I 1608 GTATAC GTA^TAC BstYI 1546 GGATCT [GA]^GATC[CT] BstZ17I 1608 GTATAC GTA^TAC CelII 932 GCTCAGC GC^T[ACGT]AGC DrdI 1105 GACAGATATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 35 TGGCCA [CT]^GGCC[GA] EcoRI 1311 GAATTC G^AATTC EcoT14I 2072 CCAAGG C^C[AT][AT]GG HaeII 72 GGCGCT [GA]GCGC^[CT] HapII 1131 CCGG C^CGG HincII 529 GTTAAC GT[CT]^[GA]AC HindII 529 GTTAAC GT[CT]^[GA]AC HpaI 529 GTTAAC GTT^AAC HpaII 1131 CCGG C^CGG MamI 1029 GATGCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 1546 GGATCT [GA]^GATC[CT] MluNI 35 TGGCCA TGG^CCA MscI 35 TGGCCA TGG^CCA MslI 334 CATTATGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 1131 CCGG C^CGG NciI 1131 CCGGG CC^[GC]GG NdeI 1673 CATATG CA^TATG PflMI 2072 CCAAGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG RcaI 1185 TCATGA T^CATGA SexAI 807 ACCAGGT A^CC[AT]GGT SmlI 497 CTCAAG C^T[CT][GA]AG SphI 1523 GCATGC GCATG^C StuI 2075 AGGCCT AGG^CCT StyI 2072 CCAAGG C^C[AT][AT]GG Van91I 2072 CCAAGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 943 CCAGATAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 1546 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### AlwI 1699 GATCC GGATC(4/5) 1546 GGATC AseI 391 ATTAAT AT^TAAT 1348 ATTAAT AsnI 391 ATTAAT AT^TAAT 1348 ATTAAT AspHI 1601 GAGCAC G[AT]GC[AT]^C 1774 GTGCTC AvaII 988 GGACC G^G[AT]CC 2064 GGACC BsaI 794 GAGACC GGTCTC(1/5) 1230 GAGACC BsgI 788 GTGCAG GTGCAG(16/14) 2122 GTGCAG BsiHKAI 1601 GAGCAC G[AT]GC[AT]^C 1774 GTGCTC BsmFI 569 GGGAC GGGAC(10/14) 1133 GGGAC Bsp1407I 1396 TGTACA T^GTACA 1409 TGTACA BsrGI 1396 TGTACA T^GTACA 1409 TGTACA BsrI 1000 ACTGG ACTGG(1/-1) 294 CCAGT BstEII 811 GGTTACC G^GT[ACGT]ACC 1126 GGTCACC BstPI 811 GGTTACC G^GT[ACGT]ACC 1126 GGTCACC BstXI 727 CCAGATGGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1575 CCAACGACTTGG DraI 556 TTTAAA TTT^AAA 1708 TTTAAA EarI 112 CTCTTC CTCTTC(1/4) 2091 GAAGAG EcoNI 439 CCTCCTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1450 CCTGATGAAGG EcoO65I 811 GGTTACC G^GT[ACGT]ACC 1126 GGTCACC FauI 51 CCCGC CCCGC(4/6) 85 GCGGG Ksp632I 112 CTCTTC CTCTTC(1/4) 2091 GAAGAG MfeI 582 CAATTG C^AATTG 887 CAATTG MunI 582 CAATTG C^AATTG 887 CAATTG NheI 327 GCTAGC G^CTAGC 1758 GCTAGC NspI 1523 GCATGC [GA]CATG^[CT] 1971 GCATGT PshBI 391 ATTAAT AT^TAAT 1348 ATTAAT PstI 1901 CTGCAG CTGCA^G 2132 CTGCAG SspBI 1396 TGTACA T^GTACA 1409 TGTACA TaqI 1737 TCGA T^CGA 1791 TCGA Tsp45I 1054 GTGAC ^GT[GC]AC 1127 GTCAC TthHB8I 1737 TCGA T^CGA 1791 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 64 enzymes) # ######################################### AccII AciI AcsI AfaI AluI ApoI Asp700I BbsI BbvI BfaI BmyI BpmI BpuAI BsaJI BseRI BsiYI BslI BsmAI Bsp1286I BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII EcoRII Fnu4HI FokI HaeIII HhaI HinP1I HinfI HphI ItaI MaeI MaeIII MboI MboII MnlI MseI MspA1I MvaI MvnI MwoI NdeII NlaIII NlaIV PleI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI TfiI Tru9I TseI Tsp509I TspRI XmnI