(Circular) (Six-base) MAPSORT of: pFN21AE4567.seq Check: 6237 from: 1 to: 6862 pFN21AE4567 6862 bp With 182 enzymes: SgfI * July 6, 2012 17:34 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4672 278 Size: 53 83 186 936 3136 2468 Fragments arranged by size: 3136 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3614 4205 1937 Size: 1677 591 4594 Fragments arranged by size: 4594 1677 591 Acc65I G'GTAC_C Cuts at: 4183 4183 Size: 6862 AclI AA'CG_TT Cuts at: 1563 4989 5362 1563 Size: 3426 373 3063 Fragments arranged by size: 3426 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2571 3582 4925 6309 6577 1632 Size: 939 1011 1343 1384 268 1917 Fragments arranged by size: 1917 1384 1343 1011 939 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6665 AflIII A'CryG_T Cuts at: 1203 5761 6738 1203 Size: 4558 977 1327 Fragments arranged by size: 4558 1327 977 AgeI A'CCGG_T Cuts at: 5693 5693 Size: 6862 AhdI GACnn_n'nnGTC Cuts at: 5591 5591 Size: 6862 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6862 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4152 1184 Size: 2968 3894 AlwNI CAG_nnn'CTG Cuts at: 2861 4605 6177 2861 Size: 1744 1572 3546 Fragments arranged by size: 3546 1744 1572 ApaLI G'TGCA_C Cuts at: 4919 6075 4919 Size: 1156 5706 ApoI r'AATT_y Cuts at: 1493 2289 2309 3066 3278 3316 3638 3916 4032 Size: 796 20 757 212 38 322 278 116 Cuts at: 4032 4171 4397 4549 6658 1493 Size: 139 226 152 2109 1697 Fragments arranged by size: 2109 1697 796 757 322 278 226 212 152 139 116 38 20 AseI AT'TA_AT Cuts at: 160 1032 2397 3354 5416 160 Size: 872 1365 957 2062 1606 Fragments arranged by size: 2062 1606 1365 957 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6862 AvaI C'yCGr_G Cuts at: 1870 1943 4187 1870 Size: 73 2244 4545 Fragments arranged by size: 4545 2244 73 BamHI G'GATC_C Cuts at: 4192 4559 4192 Size: 367 6495 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4183 5638 618 Size: 325 217 627 2396 1455 1842 Fragments arranged by size: 2396 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 4181 1391 Size: 2790 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2559 2861 3278 3878 928 Size: 971 660 302 417 600 3912 Fragments arranged by size: 3912 971 660 600 417 302 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5053 5087 1903 Size: 34 3116 34 3678 Fragments arranged by size: 3678 3116 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2891 4754 5964 2891 Size: 1863 1210 3789 Fragments arranged by size: 3789 1863 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6862 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2660 5473 136 Size: 107 122 71 631 605 988 2813 1525 Fragments arranged by size: 2813 1525 988 631 605 122 107 71 BglII A'GATC_T Cuts at: 6857 6857 Size: 6862 BlpI GC'TnA_GC Cuts at: 2938 4279 2938 Size: 1341 5521 Bme1580I G_kGCm'C Cuts at: 1790 2982 4923 6079 1790 Size: 1192 1941 1156 2573 Fragments arranged by size: 2573 1941 1192 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6862 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2293 4593 5551 Size: 416 53 83 399 24 876 2300 958 Cuts at: 5551 442 Size: 1753 Fragments arranged by size: 2300 1753 958 876 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2336 3767 1056 Size: 1280 1431 4151 Fragments arranged by size: 4151 1431 1280 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2699 2822 2987 2699 Size: 123 165 6574 Fragments arranged by size: 6574 165 123 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2433 2772 2980 3938 5522 1239 Size: 1194 339 208 958 1584 2579 Fragments arranged by size: 2579 1584 1194 958 339 208 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2487 4987 5852 6150 2487 Size: 2500 865 298 3199 Fragments arranged by size: 3199 2500 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2793 3229 5525 882 Size: 617 1294 436 2296 2219 Fragments arranged by size: 2296 2219 1294 617 436 BsaAI yAC'GTr Cuts at: 493 1204 4653 493 Size: 711 3449 2702 Fragments arranged by size: 3449 2702 711 BsaBI GATnn'nnATC Cuts at: 3038 6856 3038 Size: 3818 3044 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4669 5051 5686 275 Size: 53 83 186 936 3136 382 635 1451 Fragments arranged by size: 3136 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5295 5693 5967 6114 6525 5295 Size: 398 274 147 411 5632 Fragments arranged by size: 5632 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2745 2775 2901 2931 2953 Size: 30 165 30 1342 30 126 30 22 Cuts at: 2953 2983 3168 3198 3738 3768 1178 Size: 30 185 30 540 30 4272 Fragments arranged by size: 4272 1342 540 185 165 126 30 30 30 30 30 30 30 22 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2096 2436 2490 3108 3217 3326 3759 1186 Size: 910 340 54 618 109 109 433 4289 Fragments arranged by size: 4289 910 618 433 340 109 109 54 BseYI C'CCAG_C Cuts at: 2072 2655 2821 3264 6065 6561 2072 Size: 583 166 443 2801 496 2373 Fragments arranged by size: 2801 2373 583 496 443 166 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2814 4148 1163 Size: 573 1078 1334 3877 Fragments arranged by size: 3877 1334 1078 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4312 5073 6101 1540 Size: 275 186 2311 761 1028 2301 Fragments arranged by size: 2311 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3610 3783 4923 5008 6079 3610 Size: 173 1140 85 1071 4393 Fragments arranged by size: 4393 1140 1071 173 85 BsmI GAATG_Cn' Cuts at: 2514 4373 4466 2514 Size: 1859 93 4910 Fragments arranged by size: 4910 1859 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2982 3610 3783 4181 4923 5008 6079 Size: 399 1192 628 173 398 742 85 1071 Cuts at: 6079 1391 Size: 2174 Fragments arranged by size: 2174 1192 1071 742 628 399 398 173 85 BspEI T'CCGG_A Cuts at: 6525 6525 Size: 6862 BspHI T'CATG_A Cuts at: 3190 4751 6481 3190 Size: 1561 1730 3571 Fragments arranged by size: 3571 1730 1561 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4217 844 Size: 3373 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4749 1394 Size: 429 2926 3507 Fragments arranged by size: 3507 2926 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5357 5531 65 Size: 1183 4109 174 1396 Fragments arranged by size: 4109 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5506 5693 1700 Size: 110 3696 187 2869 Fragments arranged by size: 3696 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3401 3414 96 Size: 3305 13 3544 Fragments arranged by size: 3544 3305 13 BssHII G'CGCG_C Cuts at: 1925 2048 1925 Size: 123 6739 BssSI C'ACGA_G Cuts at: 4922 5934 4922 Size: 1012 5850 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6862 BstBI TT'CG_AA Cuts at: 5666 5666 Size: 6862 BstEII G'GTnAC_C Cuts at: 1184 2816 3131 1184 Size: 1632 315 4915 Fragments arranged by size: 4915 1632 315 BstXI CCAn_nnnn'nTGG Cuts at: 2739 3587 2739 Size: 848 6014 BstYI r'GATC_y Cuts at: 1972 3551 4192 4559 4944 4961 6413 6499 6511 Size: 1579 641 367 385 17 1452 86 12 Cuts at: 6511 6857 1972 Size: 346 1977 Fragments arranged by size: 1977 1579 1452 641 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 3615 3615 Size: 6862 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6310 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6123 Fragments arranged by size: 6123 683 56 BtsI GCAGTG_nn' Cuts at: 3105 4378 5172 5192 3105 Size: 1273 794 20 4775 Fragments arranged by size: 4775 1273 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6862 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2282 2317 529 Size: 35 1718 35 5074 Fragments arranged by size: 5074 1718 35 35 DraI TTT'AAA Cuts at: 2563 3715 4166 2563 Size: 1152 451 5259 Fragments arranged by size: 5259 1152 451 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3116 5869 809 Size: 507 1800 2753 1802 Fragments arranged by size: 2753 1802 1800 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2040 4309 5198 Size: 54 1425 50 275 18 210 2269 889 Cuts at: 5198 6827 8 Size: 1629 43 Fragments arranged by size: 2269 1629 1425 889 275 210 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4309 1537 Size: 275 2497 4090 Fragments arranged by size: 4090 2497 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2123 4091 4792 1353 Size: 770 1968 701 3423 Fragments arranged by size: 3423 1968 770 701 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5449 5823 5969 387 Size: 1064 138 3860 374 146 1280 Fragments arranged by size: 3860 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2433 2571 2772 2980 3582 3938 4925 Size: 393 801 138 201 208 602 356 987 Cuts at: 4925 5522 6309 6577 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 987 801 787 602 597 393 356 268 208 201 138 EcoNI CCTnn'n_nnAGG Cuts at: 2448 3459 2448 Size: 1011 5851 EcoRI G'AATT_C Cuts at: 3316 4171 4549 3316 Size: 855 378 5629 Fragments arranged by size: 5629 855 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2239 2239 Size: 6862 FspI TGC'GCA Cuts at: 5368 5368 Size: 6862 HaeII r_GCGC'y Cuts at: 1678 2081 6009 1678 Size: 403 3928 2531 Fragments arranged by size: 3928 2531 403 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2745 2777 2953 2985 3070 Size: 32 135 32 1173 32 176 32 85 Cuts at: 3070 3102 5503 5535 5577 5609 1373 Size: 32 2401 32 42 32 2626 Fragments arranged by size: 2626 2401 1173 176 135 85 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2536 4206 4452 669 Size: 988 281 598 1670 246 3079 Fragments arranged by size: 3079 1670 988 598 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6862 HpaI GTT'AAC Cuts at: 2536 4452 2536 Size: 1916 4946 KpnI G_GTAC'C Cuts at: 4187 4187 Size: 6862 MfeI C'AATT_G Cuts at: 2587 2892 4461 2587 Size: 305 1569 4988 Fragments arranged by size: 4988 1569 305 MluI A'CGCG_T Cuts at: 6738 6738 Size: 6862 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2715 2720 2971 3473 3604 5976 6160 1305 Size: 1410 5 251 502 131 2372 184 2007 Fragments arranged by size: 2372 2007 1410 502 251 184 131 5 MscI TGG'CCA Cuts at: 10 64 1489 1832 2042 10 Size: 54 1425 343 210 4830 Fragments arranged by size: 4830 1425 343 210 54 MslI CAynn'nnrTG Cuts at: 518 1166 2343 4820 5179 5338 518 Size: 648 1177 2477 359 159 2042 Fragments arranged by size: 2477 2042 1177 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2659 2958 3182 3409 3645 4136 4278 4955 Size: 1053 299 224 227 236 491 142 677 Cuts at: 4955 6103 6348 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 1053 677 491 299 245 236 227 224 142 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6310 NdeI CA'TA_TG Cuts at: 387 3679 387 Size: 3292 3570 NheI G'CTAG_C Cuts at: 1052 2332 3763 1052 Size: 1280 1431 4151 Fragments arranged by size: 4151 1431 1280 NotI GC'GGCC_GC Cuts at: 4309 4309 Size: 6862 NspI r_CATG'y Cuts at: 1132 1229 3532 3980 4220 5765 1132 Size: 97 2303 448 240 1545 2229 Fragments arranged by size: 2303 2229 1545 448 240 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6862 PciI A'CATG_T Cuts at: 5761 5761 Size: 6862 PflMI CCAn_nnn'nTGG Cuts at: 1108 4083 1108 Size: 2975 3887 PfoI T'CCnGG_A Cuts at: 2449 2749 3954 2449 Size: 300 1205 5357 Fragments arranged by size: 5357 1205 300 PmeI GTTT'AAAC Cuts at: 4166 4166 Size: 6862 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4931 4963 6444 6476 4931 Size: 32 1481 32 5317 Fragments arranged by size: 5317 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6862 PsiI TTA'TAA Cuts at: 2132 4432 2132 Size: 2300 4562 PstI C_TGCA'G Cuts at: 830 3910 4141 4214 830 Size: 3080 231 73 3478 Fragments arranged by size: 3478 3080 231 73 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6862 PvuII CAG'CTG Cuts at: 2659 2958 3182 4136 2659 Size: 299 224 954 5385 Fragments arranged by size: 5385 954 299 224 SalI G'TCGA_C Cuts at: 1936 4204 1936 Size: 2268 4594 SbfI CC_TGCA'GG Cuts at: 4214 4214 Size: 6862 ScaI AGT'ACT Cuts at: 5110 6822 5110 Size: 1712 5150 SexAI A'CCwGG_T Cuts at: 2812 2812 Size: 6862 SfcI C'TryA_G Cuts at: 826 1045 3369 3807 3838 3906 4137 4210 5345 Size: 219 2324 438 31 68 231 73 1135 Cuts at: 5345 6026 6217 826 Size: 681 191 1471 Fragments arranged by size: 2324 1471 1135 681 438 231 219 191 73 68 31 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6862 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6862 SmaI CCC'GGG Cuts at: 1872 4189 1872 Size: 2317 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2502 4966 5867 6129 820 Size: 197 926 559 2464 901 262 1553 Fragments arranged by size: 2464 1553 926 901 559 262 197 SnaBI TAC'GTA Cuts at: 493 4653 493 Size: 4160 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6862 SphI G_CATG'C Cuts at: 1132 3532 4220 1132 Size: 2400 688 3774 Fragments arranged by size: 3774 2400 688 SspI AAT'ATT Cuts at: 5 52 4786 5 Size: 47 4734 2081 Fragments arranged by size: 4734 2081 47 StuI AGG'CCT Cuts at: 1517 4082 1517 Size: 2565 4297 StyI C'CwwG_G Cuts at: 513 1065 4077 4301 513 Size: 552 3012 224 3074 Fragments arranged by size: 3074 3012 552 224 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2921 3136 4889 4906 5059 5244 5689 Size: 1255 1341 215 1753 17 153 185 445 Cuts at: 5689 325 Size: 1498 Fragments arranged by size: 1753 1498 1341 1255 445 215 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3401 3414 5108 Size: 274 80 33 51 1443 1424 13 1694 Cuts at: 5108 6820 96 Size: 1712 138 Fragments arranged by size: 1712 1694 1443 1424 274 138 80 51 33 13 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2901 2933 3738 3770 6822 6854 2901 Size: 32 805 32 3052 32 2909 Fragments arranged by size: 3052 2909 805 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6862 XbaI T'CTAG_A Cuts at: 4198 4198 Size: 6862 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2955 2955 Size: 6862 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6862 XmaI C'CCGG_G Cuts at: 1870 4187 1870 Size: 2317 4545 XmnI GAAnn'nnTTC Cuts at: 2627 3013 3595 4991 2627 Size: 386 582 1396 4498 Fragments arranged by size: 4498 1396 582 386 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4670 276 Size: 53 83 186 936 3136 2468 Fragments arranged by size: 3136 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI Bsu36I DraIII EcoICRI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI