(Linear) (Six-base) MAPSORT of: KIEE4567.seq Check: 26 from: 1 to: 2157 KIEE4567.orf With 182 enzymes: SgfI * July 3, 2012 19:01 .. AccI GT'mk_AC Cuts at: 0 1609 2157 Size: 1609 548 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 566 1577 2157 Size: 566 1011 580 Fragments arranged by size: 1011 580 566 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 2147 2157 Size: 2147 10 AlwNI CAG_nnn'CTG Cuts at: 0 856 2157 Size: 856 1301 ApoI r'AATT_y Cuts at: 0 284 304 1061 1273 1311 1633 1911 2027 Size: 284 20 757 212 38 322 278 116 Cuts at: 2027 2157 Size: 130 Fragments arranged by size: 757 322 284 278 212 130 116 38 20 AseI AT'TA_AT Cuts at: 0 392 1349 2157 Size: 392 957 808 Fragments arranged by size: 957 808 392 BbsI GAAGACnn'nnnn_ Cuts at: 0 554 856 1273 1873 2157 Size: 554 302 417 600 284 Fragments arranged by size: 600 554 417 302 284 BciVI GTATCCnnnnn_n' Cuts at: 0 886 2157 Size: 886 1271 BglI GCCn_nnn'nGGC Cuts at: 0 655 2157 Size: 655 1502 BlpI GC'TnA_GC Cuts at: 0 933 2157 Size: 933 1224 Bme1580I G_kGCm'C Cuts at: 0 977 2157 Size: 977 1180 BmrI ACTGGGnnnn_n' Cuts at: 0 288 2157 Size: 288 1869 BmtI G_CTAG'C Cuts at: 0 331 1762 2157 Size: 331 1431 395 Fragments arranged by size: 1431 395 331 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 694 817 982 2157 Size: 694 123 165 1175 Fragments arranged by size: 1175 694 165 123 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 428 767 975 1933 2157 Size: 428 339 208 958 224 Fragments arranged by size: 958 428 339 224 208 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 482 2157 Size: 482 1675 BsaI GGTCTCn'nnnn_ Cuts at: 0 788 1224 2157 Size: 788 436 933 Fragments arranged by size: 933 788 436 BsaBI GATnn'nnATC Cuts at: 0 1033 2157 Size: 1033 1124 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 740 770 896 926 948 978 1163 1193 Size: 740 30 126 30 22 30 185 30 Cuts at: 1193 1733 1763 2157 Size: 540 30 394 Fragments arranged by size: 740 540 394 185 126 30 30 30 30 30 22 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 91 431 485 1103 1212 1321 1754 2157 Size: 91 340 54 618 109 109 433 403 Fragments arranged by size: 618 433 403 340 109 109 91 54 BseYI C'CCAG_C Cuts at: 0 67 650 816 1259 2157 Size: 67 583 166 443 898 Fragments arranged by size: 898 583 443 166 67 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 809 2143 2157 Size: 809 1334 14 Fragments arranged by size: 1334 809 14 BsiHKAI G_wGCw'C Cuts at: 0 1605 1778 2157 Size: 1605 173 379 Fragments arranged by size: 1605 379 173 BsmI GAATG_Cn' Cuts at: 0 509 2157 Size: 509 1648 Bsp1286I G_dGCh'C Cuts at: 0 977 1605 1778 2157 Size: 977 628 173 379 Fragments arranged by size: 977 628 379 173 BspHI T'CATG_A Cuts at: 0 1185 2157 Size: 1185 972 BsrGI T'GTAC_A Cuts at: 0 1396 1409 2157 Size: 1396 13 748 Fragments arranged by size: 1396 748 13 BssHII G'CGCG_C Cuts at: 0 43 2157 Size: 43 2114 BstEII G'GTnAC_C Cuts at: 0 811 1126 2157 Size: 811 315 1031 Fragments arranged by size: 1031 811 315 BstXI CCAn_nnnn'nTGG Cuts at: 0 734 1582 2157 Size: 734 848 575 Fragments arranged by size: 848 734 575 BstYI r'GATC_y Cuts at: 0 1546 2157 Size: 1546 611 BstZ17I GTA'TAC Cuts at: 0 1610 2157 Size: 1610 547 BtsI GCAGTG_nn' Cuts at: 0 1100 2157 Size: 1100 1057 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 277 312 2157 Size: 277 35 1845 Fragments arranged by size: 1845 277 35 DraI TTT'AAA Cuts at: 0 558 1710 2157 Size: 558 1152 447 Fragments arranged by size: 1152 558 447 DrdI GACnn_nn'nnGTC Cuts at: 0 1111 2157 Size: 1111 1046 EaeI y'GGCC_r Cuts at: 0 35 2157 Size: 35 2122 EarI CTCTTCn'nnn_ Cuts at: 0 118 2086 2157 Size: 118 1968 71 Fragments arranged by size: 1968 118 71 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 428 566 767 975 1577 1933 2157 Size: 428 138 201 208 602 356 224 Fragments arranged by size: 602 428 356 224 208 201 138 EcoNI CCTnn'n_nnAGG Cuts at: 0 443 1454 2157 Size: 443 1011 703 Fragments arranged by size: 1011 703 443 EcoRI G'AATT_C Cuts at: 0 1311 2157 Size: 1311 846 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 234 2157 Size: 234 1923 HaeII r_GCGC'y Cuts at: 0 76 2157 Size: 76 2081 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 740 772 948 980 1065 1097 2157 Size: 740 32 176 32 85 32 1060 Fragments arranged by size: 1060 740 176 85 32 32 32 HincII GTy'rAC Cuts at: 0 531 2157 Size: 531 1626 HpaI GTT'AAC Cuts at: 0 531 2157 Size: 531 1626 MfeI C'AATT_G Cuts at: 0 582 887 2157 Size: 582 305 1270 Fragments arranged by size: 1270 582 305 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 710 715 966 1468 1599 2157 Size: 710 5 251 502 131 558 Fragments arranged by size: 710 558 502 251 131 5 MscI TGG'CCA Cuts at: 0 37 2157 Size: 37 2120 MslI CAynn'nnrTG Cuts at: 0 338 2157 Size: 338 1819 MspA1I CmG'CkG Cuts at: 0 654 953 1177 1404 1640 2131 2157 Size: 654 299 224 227 236 491 26 Fragments arranged by size: 654 491 299 236 227 224 26 NdeI CA'TA_TG Cuts at: 0 1674 2157 Size: 1674 483 NheI G'CTAG_C Cuts at: 0 327 1758 2157 Size: 327 1431 399 Fragments arranged by size: 1431 399 327 NspI r_CATG'y Cuts at: 0 1527 1975 2157 Size: 1527 448 182 Fragments arranged by size: 1527 448 182 PflMI CCAn_nnn'nTGG Cuts at: 0 2078 2157 Size: 2078 79 PfoI T'CCnGG_A Cuts at: 0 444 744 1949 2157 Size: 444 300 1205 208 Fragments arranged by size: 1205 444 300 208 PsiI TTA'TAA Cuts at: 0 127 2157 Size: 127 2030 PstI C_TGCA'G Cuts at: 0 1905 2136 2157 Size: 1905 231 21 Fragments arranged by size: 1905 231 21 PvuII CAG'CTG Cuts at: 0 654 953 1177 2131 2157 Size: 654 299 224 954 26 Fragments arranged by size: 954 654 299 224 26 SexAI A'CCwGG_T Cuts at: 0 807 2157 Size: 807 1350 SfcI C'TryA_G Cuts at: 0 1364 1802 1833 1901 2132 2157 Size: 1364 438 31 68 231 25 Fragments arranged by size: 1364 438 231 68 31 25 SmlI C'TyrA_G Cuts at: 0 497 2157 Size: 497 1660 SphI G_CATG'C Cuts at: 0 1527 2157 Size: 1527 630 StuI AGG'CCT Cuts at: 0 2077 2157 Size: 2077 80 StyI C'CwwG_G Cuts at: 0 2072 2157 Size: 2072 85 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 916 1131 2157 Size: 916 215 1026 Fragments arranged by size: 1026 916 215 TatI w'GTAC_w Cuts at: 0 1396 1409 2157 Size: 1396 13 748 Fragments arranged by size: 1396 748 13 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 896 928 1733 1765 2157 Size: 896 32 805 32 392 Fragments arranged by size: 896 805 392 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 950 2157 Size: 950 1207 XmnI GAAnn'nnTTC Cuts at: 0 622 1008 1590 2157 Size: 622 386 582 567 Fragments arranged by size: 622 582 567 386 Enzymes that do cut: AccI AcuI AlfI AlwNI ApoI AseI BbsI BciVI BglI BlpI Bme1580I BmrI BmtI BplI BpmI BpuEI BsaI BsaBI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI BsrGI BssHII BstEII BstXI BstYI BstZ17I BtsI CspCI DraI DrdI EaeI EarI Eco57MI EcoNI EcoRI FalI HaeII Hin4I HincII HpaI MfeI MmeI MscI MslI MspA1I NdeI NheI NspI PflMI PfoI PsiI PstI PvuII SexAI SfcI SmlI SphI StuI StyI TaqII TatI TstI XcmI XmnI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AloI ApaI ApaLI AscI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI BbvCI BcgI BclI BfrBI BglII BmgBI Bpu10I BsaAI BsaHI BsaWI BsiEI BsiWI BsmBI BspEI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI Bsu36I BtgI BtgZI ClaI DraIII EagI EciI EcoICRI EcoO109I EcoRV FseI FspI FspAI HindIII KasI KpnI MluI NaeI NarI NcoI NgoMIV NotI NruI NsiI PacI PasI PciI PmeI PmlI PpiI PpuMI PshAI PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI SwaI Tth111I XbaI XhoI XmaI ZraI