Restriction map for
pFN21AE5711
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# NO CUT ENZYMES ( 107 enzymes) #
##################################
AatII Acc65I AccII AcyI AgeI
AhdI Alw44I Aor51HI ApaLI AscI
AseI AsnI Asp718I AspEI AviII
AvrII BbeI BbrPI BcgI BciVI
BclI BglII BlnI BlpI Bpu1102I
BsaHI BsaI BsiEI BsiWI Bsp1407I
BspDI BspHI BsrBI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstUI BstZ17I CelII CfoI ClaI
CpoI DraI EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I EcoRV EcoT22I
FbaI FseI FspI HaeII HhaI
Hin1I HinP1I HindIII HpaI KasI
KpnI KspI MfeI MluI MunI
MvnI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PinAI PmaCI PmeI PmlI
PshBI PvuI RcaI RsrII SacII
SalI ScaI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
SspBI SspI StuI SwaI XbaI
XmaCI XmaI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccIII 1550 TCCGGA T^CCGGA
AclI 2400 AACGTT AA^CGTT
AcsI 2116 GAATTC [GA]^AATT[CT]
AflII 1872 CTTAAG C^TTAAG
ApoI 2116 GAATTC [GA]^AATT[CT]
Asp700I 1659 GAACATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1781 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 1670 TGGCCA TGG^CCA
BamHI 826 GGATCC G^GATCC
BanI 985 GGTGCC G^G[CT][GA]CC
BbvCI 1143 CCTCAGC CCTCAGC(-5/-2)
BfaI 2035 CTAG C^TAG
BfrI 1872 CTTAAG C^TTAAG
BglI 2057 GCCCACAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 1269 CACGTA [CT]AC^GT[GA]
BsaBI 1942 GATGGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1550 TCCGGA [AT]^CCGG[AT]
BseAI 1550 TCCGGA T^CCGGA
BsmBI 175 GAGACG CGTCTC(1/5)
BspEI 1550 TCCGGA T^CCGGA
BspLU11I 1661 ACATGT A^CATGT
BspMI 1487 ACCTGC ACCTGC(4/8)
BsrDI 617 GCAATG GCAATG(2/0)
BsrFI 1130 ACCGGC [GA]^CCGG[CT]
BstAPI 27 GCAGGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 2299 CCAGACCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 1130 ACCGGC [GA]^CCGG[CT]
DraIII 726 CACAGCGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoRI 2116 GAATTC G^AATTC
HincII 1330 GTCAAC GT[CT]^[GA]AC
HindII 1330 GTCAAC GT[CT]^[GA]AC
MaeI 2035 CTAG C^TAG
MamI 1942 GATGGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1670 TGGCCA TGG^CCA
MroI 1550 TCCGGA T^CCGGA
MscI 1670 TGGCCA TGG^CCA
MseI 1873 TTAA T^TAA
NcoI 543 CCATGG C^CATGG
PflFI 1781 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 531 CCAGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 337 GACATCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 2400 AACGTT AA^CGTT
SapI 405 GCTCTTC GCTCTTC(1/4)
SbfI 1824 CCTGCAGG CCTGCA^GG
SexAI 1169 ACCTGGT A^CC[AT]GGT
Sse8387I 1824 CCTGCAGG CCTGCA^GG
Tru9I 1873 TTAA T^TAA
Tsp509I 2117 AATT ^AATT
Tth111I 1781 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 531 CCAGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 1659 GAACATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 13 enzymes) #
###################################
AflIII 1268 ACACGT A^C[GA][CT]GT
1661 ACATGT
BsmI 3 GAATGC GAATGC(1/-1)
1323 GAATGC
DsaI 543 CCATGG C^C[GA][CT]GG
2385 CCGTGG
EaeI 1670 TGGCCA [CT]^GGCC[GA]
1916 CGGCCA
EcoNI 861 CCTTCAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1749 CCTGAAACAGG
EcoT14I 543 CCATGG C^C[AT][AT]GG
1421 CCAAGG
MaeII 1270 ACGT A^CGT
2401 ACGT
MaeIII 1624 GTCAC ^GT[ACGT]AC
2293 GTGAC
MslI 387 CACCAAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
891 CACTCGAGTG
PaeR7I 893 CTCGAG C^TCGAG
1225 CTCGAG
StyI 543 CCATGG C^C[AT][AT]GG
1421 CCAAGG
Tsp45I 1624 GTCAC ^GT[GC]AC
2293 GTGAC
XhoI 893 CTCGAG C^TCGAG
1225 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 87 enzymes) #
#########################################
AccI AciI AfaI AluI AlwI
AlwNI AocI ApaI AspHI AvaI
AvaII BanII BbsI BbvI BcnI
BmyI BpmI BpuAI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrI BssKI
BstF5I BstNI BstYI Bsu36I Cac8I
Cfr13I DdeI DpnII DraII DrdI
EarI Eco81I EcoO109I EcoRII FauI
Fnu4HI FokI HaeIII HapII HgaI
HinfI HpaII HphI ItaI Ksp632I
MboI MboII MflI MnlI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
TaqI TfiI TseI TspRI TthHB8I
XcmI XhoII