(Circular) (Six-base) MAPSORT of: pFN21AE5711.seq Check: 3090 from: 1 to: 7120 pFN21AE5711 7120 bp With 182 enzymes: SgfI * December 8, 2014 13:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4930 278 Size: 53 83 186 936 3394 2468 Fragments arranged by size: 3394 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2350 2682 2925 4463 1937 Size: 413 332 243 1538 4594 Fragments arranged by size: 4594 1538 413 332 243 Acc65I G'GTAC_C Cuts at: 4441 4441 Size: 7120 AclI AA'CG_TT Cuts at: 1563 4406 5247 5620 1563 Size: 2843 841 373 3063 Fragments arranged by size: 3063 2843 841 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2184 2852 5183 6567 6835 1632 Size: 552 668 2331 1384 268 1917 Fragments arranged by size: 2331 1917 1384 668 552 268 AflII C'TTAA_G Cuts at: 820 1017 3877 820 Size: 197 2860 4063 Fragments arranged by size: 4063 2860 197 AflIII A'CryG_T Cuts at: 1203 3273 3666 6019 6996 1203 Size: 2070 393 2353 977 1327 Fragments arranged by size: 2353 2070 1327 977 393 AgeI A'CCGG_T Cuts at: 5951 5951 Size: 7120 AhdI GACnn_n'nnGTC Cuts at: 5849 5849 Size: 7120 AleI CACnn'nnGTG Cuts at: 1166 2396 2900 1166 Size: 1230 504 5386 Fragments arranged by size: 5386 1230 504 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7120 AlwNI CAG_nnn'CTG Cuts at: 2038 2211 2435 2542 3045 3159 3997 4088 4863 Size: 173 224 107 503 114 838 91 775 Cuts at: 4863 6435 2038 Size: 1572 2723 Fragments arranged by size: 2723 1572 838 775 503 224 173 114 107 91 ApaI G_GGCC'C Cuts at: 2495 2721 2866 3617 2495 Size: 226 145 751 5998 Fragments arranged by size: 5998 751 226 145 ApaLI G'TGCA_C Cuts at: 5177 6333 5177 Size: 1156 5964 ApoI r'AATT_y Cuts at: 1493 4121 4429 4655 4807 6916 1493 Size: 2628 308 226 152 2109 1697 Fragments arranged by size: 2628 2109 1697 308 226 152 AseI AT'TA_AT Cuts at: 160 1032 5674 160 Size: 872 4642 1606 Fragments arranged by size: 4642 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7120 AvaI C'yCGr_G Cuts at: 1870 1943 2898 3230 3566 4445 1870 Size: 73 955 332 336 879 4545 Fragments arranged by size: 4545 955 879 336 332 73 BamHI G'GATC_C Cuts at: 2831 4450 4817 2831 Size: 1619 367 5134 Fragments arranged by size: 5134 1619 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2990 4441 5896 618 Size: 325 217 627 1203 1451 1455 1842 Fragments arranged by size: 1842 1455 1451 1203 627 325 217 BanII G_rGCy'C Cuts at: 1391 2412 2495 2721 2866 3126 3617 3918 4439 Size: 1021 83 226 145 260 491 301 521 Cuts at: 4439 1391 Size: 4072 Fragments arranged by size: 4072 1021 521 491 301 260 226 145 83 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2652 3079 3631 3640 928 Size: 971 753 427 552 9 4408 Fragments arranged by size: 4408 971 753 552 427 9 BbvCI CC'TCA_GC Cuts at: 3149 3149 Size: 7120 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5311 5345 1903 Size: 34 3374 34 3678 Fragments arranged by size: 3678 3374 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5012 6222 5012 Size: 1210 5910 BclI T'GATC_A Cuts at: 1548 1548 Size: 7120 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4068 5731 136 Size: 107 122 71 631 605 2396 1663 1525 Fragments arranged by size: 2396 1663 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7115 7115 Size: 7120 BlpI GC'TnA_GC Cuts at: 4537 4537 Size: 7120 Bme1580I G_kGCm'C Cuts at: 1790 2495 2721 2866 2897 3617 5181 6337 1790 Size: 705 226 145 31 720 1564 1156 2573 Fragments arranged by size: 2573 1564 1156 720 705 226 145 31 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7120 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2788 3055 3896 Size: 416 53 83 399 24 1371 267 841 Cuts at: 3896 4851 5809 442 Size: 955 958 1753 Fragments arranged by size: 1753 1371 958 955 841 416 399 267 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7120 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2110 2126 2359 2457 2673 3140 4019 4175 Size: 871 16 233 98 216 467 879 156 Cuts at: 4175 4238 5780 1239 Size: 63 1542 2579 Fragments arranged by size: 2579 1542 879 871 467 233 216 156 98 63 16 Bpu10I CC'TnA_GC Cuts at: 3149 3149 Size: 7120 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5245 6110 6408 5245 Size: 865 298 5957 Fragments arranged by size: 5957 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5783 882 Size: 617 4284 2219 Fragments arranged by size: 4284 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3276 4911 493 Size: 711 2072 1635 2702 Fragments arranged by size: 2702 2072 1635 711 BsaBI GATnn'nnATC Cuts at: 3951 7114 3951 Size: 3163 3957 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4927 5309 5944 275 Size: 53 83 186 936 3394 382 635 1451 Fragments arranged by size: 3394 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3555 5553 5951 6225 6372 6783 3555 Size: 1998 398 274 147 411 3892 Fragments arranged by size: 3892 1998 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2864 2894 3839 3869 4092 Size: 30 165 30 1461 30 945 30 223 Cuts at: 4092 4122 1178 Size: 30 4176 Fragments arranged by size: 4176 1461 945 223 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3394 3725 4388 4393 4429 1186 Size: 2208 331 663 5 36 3877 Fragments arranged by size: 3877 2208 663 331 36 5 BseYI C'CCAG_C Cuts at: 2047 2587 3396 4104 4291 6323 6819 2047 Size: 540 809 708 187 2032 496 2348 Fragments arranged by size: 2348 2032 809 708 540 496 187 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2101 2868 3839 1163 Size: 573 365 767 971 4444 Fragments arranged by size: 4444 971 767 573 365 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4570 5331 6359 1540 Size: 275 186 2569 761 1028 2301 Fragments arranged by size: 2569 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2412 3126 3660 3918 5181 5266 6337 2412 Size: 714 534 258 1263 85 1071 3195 Fragments arranged by size: 3195 1263 1071 714 534 258 85 BsmI GAATG_Cn' Cuts at: 2014 3334 4631 4724 2014 Size: 1320 1297 93 4410 Fragments arranged by size: 4410 1320 1297 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2174 2174 Size: 7120 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2412 2495 2721 2866 2897 3126 3617 Size: 399 622 83 226 145 31 229 491 Cuts at: 3617 3660 3918 4439 5181 5266 6337 1391 Size: 43 258 521 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 622 521 491 399 258 229 226 145 85 83 43 31 BspEI T'CCGG_A Cuts at: 3555 6783 3555 Size: 3228 3892 BspHI T'CATG_A Cuts at: 5009 6739 5009 Size: 1730 5390 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3501 4475 844 Size: 2657 974 3489 Fragments arranged by size: 3489 2657 974 BsrBI CCG'CTC Cuts at: 1394 1823 5007 1394 Size: 429 3184 3507 Fragments arranged by size: 3507 3184 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2629 5615 5789 65 Size: 1183 1381 2986 174 1396 Fragments arranged by size: 2986 1396 1381 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3135 5764 5951 1700 Size: 110 1325 2629 187 2869 Fragments arranged by size: 2869 2629 1325 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7120 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7120 BssSI C'ACGA_G Cuts at: 5180 6192 5180 Size: 1012 6108 BstAPI GCAn_nnn'nTGC Cuts at: 794 2038 794 Size: 1244 5876 BstBI TT'CG_AA Cuts at: 5924 5924 Size: 7120 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7120 BstXI CCAn_nnnn'nTGG Cuts at: 4311 4311 Size: 7120 BstYI r'GATC_y Cuts at: 1972 2831 3114 3849 4450 4817 5202 5219 6671 Size: 859 283 735 601 367 385 17 1452 Cuts at: 6671 6757 6769 7115 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 859 735 601 385 367 346 283 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2025 2306 2957 2025 Size: 281 651 6188 Fragments arranged by size: 6188 651 281 BtgI C'CryG_G Cuts at: 513 1065 2548 4390 513 Size: 552 1483 1842 3243 Fragments arranged by size: 3243 1842 1483 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6381 Fragments arranged by size: 6381 683 56 BtsI GCAGTG_nn' Cuts at: 4636 5430 5450 4636 Size: 794 20 6306 Fragments arranged by size: 6306 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7120 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7085 DraI TTT'AAA Cuts at: 4424 4424 Size: 7120 DraIII CAC_nnn'GTG Cuts at: 2736 2736 Size: 7120 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2200 2438 2921 3179 6127 809 Size: 507 884 238 483 258 2948 1802 Fragments arranged by size: 2948 1802 884 507 483 258 238 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3675 3921 4567 Size: 54 1425 50 275 18 1845 246 646 Cuts at: 4567 5456 7085 8 Size: 889 1629 43 Fragments arranged by size: 1845 1629 1425 889 646 275 246 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4567 1537 Size: 275 2755 4090 Fragments arranged by size: 4090 2755 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2417 3105 3347 3999 4353 5050 1353 Size: 1064 688 242 652 354 697 3423 Fragments arranged by size: 3423 1064 697 688 652 354 242 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2483 5707 6081 6227 387 Size: 1064 138 894 3224 374 146 1280 Fragments arranged by size: 3224 1280 1064 894 374 146 138 EcoICRI GAG'CTC Cuts at: 2410 3124 3916 2410 Size: 714 792 5614 Fragments arranged by size: 5614 792 714 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2110 2126 2184 2359 2457 2673 2852 Size: 393 478 16 58 175 98 216 179 Cuts at: 2852 3140 4019 4175 4238 5183 5780 6567 6835 Size: 288 879 156 63 945 597 787 268 Cuts at: 6835 1239 Size: 1524 Fragments arranged by size: 1524 945 879 787 597 478 393 288 268 216 179 175 156 98 63 58 16 EcoNI CCTnn'n_nnAGG Cuts at: 2870 3758 2870 Size: 888 6232 EcoO109I rG'GnC_Cy Cuts at: 2135 2193 2491 2718 2863 3504 3614 3826 3994 Size: 58 298 227 145 641 110 212 168 Cuts at: 3994 2135 Size: 5261 Fragments arranged by size: 5261 641 298 227 212 168 145 110 58 EcoRI G'AATT_C Cuts at: 4121 4429 4807 4121 Size: 308 378 6434 Fragments arranged by size: 6434 378 308 FspI TGC'GCA Cuts at: 5626 5626 Size: 7120 HaeII r_GCGC'y Cuts at: 1678 6267 1678 Size: 4589 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2669 2701 2979 3011 5761 Size: 32 135 32 1097 32 278 32 2750 Cuts at: 5761 5793 5835 5867 1373 Size: 32 42 32 2626 Fragments arranged by size: 2750 2626 1097 278 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3337 4464 4710 669 Size: 988 281 1399 1127 246 3079 Fragments arranged by size: 3079 1399 1127 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7120 HpaI GTT'AAC Cuts at: 4710 4710 Size: 7120 KpnI G_GTAC'C Cuts at: 4445 4445 Size: 7120 MfeI C'AATT_G Cuts at: 4719 4719 Size: 7120 MluI A'CGCG_T Cuts at: 6996 6996 Size: 7120 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4427 6234 6418 1305 Size: 3122 1807 184 2007 Fragments arranged by size: 3122 2007 1807 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3677 10 Size: 54 1425 343 1845 3453 Fragments arranged by size: 3453 1845 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2396 2900 5078 5437 5596 518 Size: 648 1230 504 2178 359 159 2042 Fragments arranged by size: 2178 2042 1230 648 504 359 159 MspA1I CmG'CkG Cuts at: 1606 2208 2273 2802 3420 4153 4295 4536 5213 Size: 602 65 529 618 733 142 241 677 Cuts at: 5213 6361 6606 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 733 677 618 602 529 245 241 142 65 NcoI C'CATG_G Cuts at: 513 1065 2548 513 Size: 552 1483 5085 Fragments arranged by size: 5085 1483 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7120 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7120 NotI GC'GGCC_GC Cuts at: 4567 4567 Size: 7120 NspI r_CATG'y Cuts at: 1132 1229 2584 3670 4132 4478 6023 1132 Size: 97 1355 1086 462 346 1545 2229 Fragments arranged by size: 2229 1545 1355 1086 462 346 97 PasI CC'CwG_GG Cuts at: 1348 2488 4311 1348 Size: 1140 1823 4157 Fragments arranged by size: 4157 1823 1140 PciI A'CATG_T Cuts at: 3666 6019 3666 Size: 2353 4767 PflMI CCAn_nnn'nTGG Cuts at: 1108 2542 1108 Size: 1434 5686 PfoI T'CCnGG_A Cuts at: 2652 2827 3117 4164 2652 Size: 175 290 1047 5608 Fragments arranged by size: 5608 1047 290 175 PmeI GTTT'AAAC Cuts at: 4424 4424 Size: 7120 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5189 5221 6702 6734 5189 Size: 32 1481 32 5575 Fragments arranged by size: 5575 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2193 3504 3826 3994 2193 Size: 1311 322 168 5319 Fragments arranged by size: 5319 1311 322 168 PshAI GACnn'nnGTC Cuts at: 1613 2346 1613 Size: 733 6387 PsiI TTA'TAA Cuts at: 4690 4690 Size: 7120 PspOMI G'GGCC_C Cuts at: 2491 2717 2862 3613 2491 Size: 226 145 751 5998 Fragments arranged by size: 5998 751 226 145 PspXI vC'TCGA_Gb Cuts at: 2898 3230 2898 Size: 332 6788 PstI C_TGCA'G Cuts at: 830 3264 3834 4152 4472 830 Size: 2434 570 318 320 3478 Fragments arranged by size: 3478 2434 570 320 318 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7120 PvuII CAG'CTG Cuts at: 2208 2273 4153 4295 2208 Size: 65 1880 142 5033 Fragments arranged by size: 5033 1880 142 65 SacI G_AGCT'C Cuts at: 2412 3126 3918 2412 Size: 714 792 5614 Fragments arranged by size: 5614 792 714 SalI G'TCGA_C Cuts at: 1936 4462 1936 Size: 2526 4594 SanDI GG'GwC_CC Cuts at: 3504 3504 Size: 7120 SapI GCTCTTCn'nnn_ Cuts at: 2417 2417 Size: 7120 SbfI CC_TGCA'GG Cuts at: 3834 4472 3834 Size: 638 6482 ScaI AGT'ACT Cuts at: 5368 7080 5368 Size: 1712 5408 SexAI A'CCwGG_T Cuts at: 3174 3174 Size: 7120 SfcI C'TryA_G Cuts at: 826 1045 2077 2797 2982 3260 3830 4148 4194 Size: 219 1032 720 185 278 570 318 46 Cuts at: 4194 4468 5603 6284 6475 826 Size: 274 1135 681 191 1471 Fragments arranged by size: 1471 1135 1032 720 681 570 318 278 274 219 191 185 46 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7120 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7120 SmaI CCC'GGG Cuts at: 1872 4447 1872 Size: 2575 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2898 3230 3877 5224 6125 6387 Size: 197 926 955 332 647 1347 901 262 Cuts at: 6387 820 Size: 1553 Fragments arranged by size: 1553 1347 955 926 901 647 332 262 197 SnaBI TAC'GTA Cuts at: 493 4911 493 Size: 4418 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7120 SphI G_CATG'C Cuts at: 1132 4478 1132 Size: 3346 3774 SspI AAT'ATT Cuts at: 5 52 5044 5 Size: 47 4992 2081 Fragments arranged by size: 4992 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7120 StyI C'CwwG_G Cuts at: 513 1065 2548 3426 4559 513 Size: 552 1483 878 1133 3074 Fragments arranged by size: 3074 1483 1133 878 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5147 5164 5317 5502 5947 325 Size: 1255 3567 17 153 185 445 1498 Fragments arranged by size: 3567 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3315 3369 5366 Size: 274 80 33 51 1443 1338 54 1997 Cuts at: 5366 7078 96 Size: 1712 138 Fragments arranged by size: 1997 1712 1443 1338 274 138 80 54 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2864 2896 7080 7112 2864 Size: 32 4184 32 2872 Fragments arranged by size: 4184 2872 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3789 1316 Size: 2473 4647 XbaI T'CTAG_A Cuts at: 4456 4456 Size: 7120 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2461 3202 3433 3607 2011 Size: 450 741 231 174 5524 Fragments arranged by size: 5524 741 450 231 174 XhoI C'TCGA_G Cuts at: 1943 2898 3230 1943 Size: 955 332 5833 Fragments arranged by size: 5833 955 332 XmaI C'CCGG_G Cuts at: 1870 4445 1870 Size: 2575 4545 XmnI GAAnn'nnTTC Cuts at: 3668 5249 3668 Size: 1581 5539 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4928 276 Size: 53 83 186 936 3394 2468 Fragments arranged by size: 3394 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstZ17I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PsrI RsrII SacII SfiI SfoI SrfI SwaI