(Linear) (Six-base) MAPSORT of: KIEE5711.seq Check: 1193 from: 1 to: 2415 KIEE5711.orf With 182 enzymes: SgfI * December 5, 2014 13:07 .. AccI GT'mk_AC Cuts at: 0 345 677 920 2415 Size: 345 332 243 1495 Fragments arranged by size: 1495 345 332 243 AclI AA'CG_TT Cuts at: 0 2401 2415 Size: 2401 14 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 179 847 2415 Size: 179 668 1568 Fragments arranged by size: 1568 668 179 AflII C'TTAA_G Cuts at: 0 1872 2415 Size: 1872 543 AflIII A'CryG_T Cuts at: 0 1268 1661 2415 Size: 1268 393 754 Fragments arranged by size: 1268 754 393 AleI CACnn'nnGTG Cuts at: 0 391 895 2415 Size: 391 504 1520 Fragments arranged by size: 1520 504 391 AlwNI CAG_nnn'CTG Cuts at: 0 33 206 430 537 1040 1154 1992 2083 Size: 33 173 224 107 503 114 838 91 Cuts at: 2083 2415 Size: 332 Fragments arranged by size: 838 503 332 224 173 114 107 91 33 ApaI G_GGCC'C Cuts at: 0 490 716 861 1612 2415 Size: 490 226 145 751 803 Fragments arranged by size: 803 751 490 226 145 ApoI r'AATT_y Cuts at: 0 2116 2415 Size: 2116 299 AvaI C'yCGr_G Cuts at: 0 893 1225 1561 2415 Size: 893 332 336 854 Fragments arranged by size: 893 854 336 332 BamHI G'GATC_C Cuts at: 0 826 2415 Size: 826 1589 BanI G'GyrC_C Cuts at: 0 985 2415 Size: 985 1430 BanII G_rGCy'C Cuts at: 0 407 490 716 861 1121 1612 1913 2415 Size: 407 83 226 145 260 491 301 502 Fragments arranged by size: 502 491 407 301 260 226 145 83 BbsI GAAGACnn'nnnn_ Cuts at: 0 647 1074 1626 1635 2415 Size: 647 427 552 9 780 Fragments arranged by size: 780 647 552 427 9 BbvCI CC'TCA_GC Cuts at: 0 1144 2415 Size: 1144 1271 BglI GCCn_nnn'nGGC Cuts at: 0 2063 2415 Size: 2063 352 Bme1580I G_kGCm'C Cuts at: 0 490 716 861 892 1612 2415 Size: 490 226 145 31 720 803 Fragments arranged by size: 803 720 490 226 145 31 BmrI ACTGGGnnnn_n' Cuts at: 0 783 1050 1891 2415 Size: 783 267 841 524 Fragments arranged by size: 841 783 524 267 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 105 121 354 452 668 1135 2014 2170 Size: 105 16 233 98 216 467 879 156 Cuts at: 2170 2233 2415 Size: 63 182 Fragments arranged by size: 879 467 233 216 182 156 105 98 63 16 Bpu10I CC'TnA_GC Cuts at: 0 1144 2415 Size: 1144 1271 BsaAI yAC'GTr Cuts at: 0 1271 2415 Size: 1271 1144 BsaBI GATnn'nnATC Cuts at: 0 1946 2415 Size: 1946 469 BsaWI w'CCGG_w Cuts at: 0 1550 2415 Size: 1550 865 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 859 889 1834 1864 2087 2117 2415 Size: 859 30 945 30 223 30 298 Fragments arranged by size: 945 859 298 223 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1389 1720 2383 2388 2415 Size: 1389 331 663 5 27 Fragments arranged by size: 1389 663 331 27 5 BseYI C'CCAG_C Cuts at: 0 42 582 1391 2099 2286 2415 Size: 42 540 809 708 187 129 Fragments arranged by size: 809 708 540 187 129 42 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 96 863 1834 2415 Size: 96 767 971 581 Fragments arranged by size: 971 767 581 96 BsiHKAI G_wGCw'C Cuts at: 0 407 1121 1655 1913 2415 Size: 407 714 534 258 502 Fragments arranged by size: 714 534 502 407 258 BsmI GAATG_Cn' Cuts at: 0 9 1329 2415 Size: 9 1320 1086 Fragments arranged by size: 1320 1086 9 BsmBI CGTCTCn'nnnn_ Cuts at: 0 169 2415 Size: 169 2246 Bsp1286I G_dGCh'C Cuts at: 0 407 490 716 861 892 1121 1612 1655 Size: 407 83 226 145 31 229 491 43 Cuts at: 1655 1913 2415 Size: 258 502 Fragments arranged by size: 502 491 407 258 229 226 145 83 43 31 BspEI T'CCGG_A Cuts at: 0 1550 2415 Size: 1550 865 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1496 2415 Size: 1496 919 BsrDI GCAATG_nn' Cuts at: 0 624 2415 Size: 624 1791 BsrFI r'CCGG_y Cuts at: 0 1130 2415 Size: 1130 1285 BstAPI GCAn_nnn'nTGC Cuts at: 0 33 2415 Size: 33 2382 BstXI CCAn_nnnn'nTGG Cuts at: 0 2306 2415 Size: 2306 109 BstYI r'GATC_y Cuts at: 0 826 1109 1844 2415 Size: 826 283 735 571 Fragments arranged by size: 826 735 571 283 Bsu36I CC'TnA_GG Cuts at: 0 20 301 952 2415 Size: 20 281 651 1463 Fragments arranged by size: 1463 651 281 20 BtgI C'CryG_G Cuts at: 0 543 2385 2415 Size: 543 1842 30 Fragments arranged by size: 1842 543 30 DraIII CAC_nnn'GTG Cuts at: 0 731 2415 Size: 731 1684 DrdI GACnn_nn'nnGTC Cuts at: 0 195 433 916 1174 2415 Size: 195 238 483 258 1241 Fragments arranged by size: 1241 483 258 238 195 EaeI y'GGCC_r Cuts at: 0 1670 1916 2415 Size: 1670 246 499 Fragments arranged by size: 1670 499 246 EarI CTCTTCn'nnn_ Cuts at: 0 412 1100 1342 1994 2348 2415 Size: 412 688 242 652 354 67 Fragments arranged by size: 688 652 412 354 242 67 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 478 2415 Size: 478 1937 EcoICRI GAG'CTC Cuts at: 0 405 1119 1911 2415 Size: 405 714 792 504 Fragments arranged by size: 792 714 504 405 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 105 121 179 354 452 668 847 1135 Size: 105 16 58 175 98 216 179 288 Cuts at: 1135 2014 2170 2233 2415 Size: 879 156 63 182 Fragments arranged by size: 879 288 216 182 179 175 156 105 98 63 58 16 EcoNI CCTnn'n_nnAGG Cuts at: 0 865 1753 2415 Size: 865 888 662 Fragments arranged by size: 888 865 662 EcoO109I rG'GnC_Cy Cuts at: 0 130 188 486 713 858 1499 1609 1821 Size: 130 58 298 227 145 641 110 212 Cuts at: 1821 1989 2415 Size: 168 426 Fragments arranged by size: 641 426 298 227 212 168 145 130 110 58 EcoRI G'AATT_C Cuts at: 0 2116 2415 Size: 2116 299 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 664 696 974 1006 2415 Size: 664 32 278 32 1409 Fragments arranged by size: 1409 664 278 32 32 HincII GTy'rAC Cuts at: 0 1332 2415 Size: 1332 1083 MscI TGG'CCA Cuts at: 0 1672 2415 Size: 1672 743 MslI CAynn'nnrTG Cuts at: 0 391 895 2415 Size: 391 504 1520 Fragments arranged by size: 1520 504 391 MspA1I CmG'CkG Cuts at: 0 203 268 797 1415 2148 2290 2415 Size: 203 65 529 618 733 142 125 Fragments arranged by size: 733 618 529 203 142 125 65 NcoI C'CATG_G Cuts at: 0 543 2415 Size: 543 1872 NspI r_CATG'y Cuts at: 0 579 1665 2127 2415 Size: 579 1086 462 288 Fragments arranged by size: 1086 579 462 288 PasI CC'CwG_GG Cuts at: 0 483 2306 2415 Size: 483 1823 109 Fragments arranged by size: 1823 483 109 PciI A'CATG_T Cuts at: 0 1661 2415 Size: 1661 754 PflMI CCAn_nnn'nTGG Cuts at: 0 537 2415 Size: 537 1878 PfoI T'CCnGG_A Cuts at: 0 647 822 1112 2159 2415 Size: 647 175 290 1047 256 Fragments arranged by size: 1047 647 290 256 175 PpuMI rG'GwC_Cy Cuts at: 0 188 1499 1821 1989 2415 Size: 188 1311 322 168 426 Fragments arranged by size: 1311 426 322 188 168 PshAI GACnn'nnGTC Cuts at: 0 341 2415 Size: 341 2074 PspOMI G'GGCC_C Cuts at: 0 486 712 857 1608 2415 Size: 486 226 145 751 807 Fragments arranged by size: 807 751 486 226 145 PspXI vC'TCGA_Gb Cuts at: 0 893 1225 2415 Size: 893 332 1190 Fragments arranged by size: 1190 893 332 PstI C_TGCA'G Cuts at: 0 1259 1829 2147 2415 Size: 1259 570 318 268 Fragments arranged by size: 1259 570 318 268 PvuII CAG'CTG Cuts at: 0 203 268 2148 2290 2415 Size: 203 65 1880 142 125 Fragments arranged by size: 1880 203 142 125 65 SacI G_AGCT'C Cuts at: 0 407 1121 1913 2415 Size: 407 714 792 502 Fragments arranged by size: 792 714 502 407 SanDI GG'GwC_CC Cuts at: 0 1499 2415 Size: 1499 916 SapI GCTCTTCn'nnn_ Cuts at: 0 412 2415 Size: 412 2003 SbfI CC_TGCA'GG Cuts at: 0 1829 2415 Size: 1829 586 SexAI A'CCwGG_T Cuts at: 0 1169 2415 Size: 1169 1246 SfcI C'TryA_G Cuts at: 0 72 792 977 1255 1825 2143 2189 2415 Size: 72 720 185 278 570 318 46 226 Fragments arranged by size: 720 570 318 278 226 185 72 46 SmlI C'TyrA_G Cuts at: 0 893 1225 1872 2415 Size: 893 332 647 543 Fragments arranged by size: 893 647 543 332 StyI C'CwwG_G Cuts at: 0 543 1421 2415 Size: 543 878 994 Fragments arranged by size: 994 878 543 TatI w'GTAC_w Cuts at: 0 1310 1364 2415 Size: 1310 54 1051 Fragments arranged by size: 1310 1051 54 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 859 891 2415 Size: 859 32 1524 Fragments arranged by size: 1524 859 32 Tth111I GACn'n_nGTC Cuts at: 0 1784 2415 Size: 1784 631 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 456 1197 1428 1602 2415 Size: 456 741 231 174 813 Fragments arranged by size: 813 741 456 231 174 XhoI C'TCGA_G Cuts at: 0 893 1225 2415 Size: 893 332 1190 Fragments arranged by size: 1190 893 332 XmnI GAAnn'nnTTC Cuts at: 0 1663 2415 Size: 1663 752 Enzymes that do cut: AccI AclI AcuI AflII AflIII AleI AlwNI ApaI ApoI AvaI BamHI BanI BanII BbsI BbvCI BglI Bme1580I BmrI BpmI Bpu10I BsaAI BsaBI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspMI BsrDI BsrFI BstAPI BstXI BstYI Bsu36I BtgI DraIII DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI Hin4I HincII MscI MslI MspA1I NcoI NspI PasI PciI PflMI PfoI PpuMI PshAI PspOMI PspXI PstI PvuII SacI SanDI SapI SbfI SexAI SfcI SmlI StyI TatI TstI Tth111I XcmI XhoI XmnI Enzymes that do not cut: AarI AatII Acc65I AfeI AgeI AhdI AlfI AloI ApaLI AscI AseI AsiSI AvrII BaeI BbeI BcgI BciVI BclI BfrBI BglII BlpI BmgBI BmtI BplI BpuEI BsaI BsaHI BsiEI BsiWI BspHI BsrBI BsrGI BssHII BssSI BstBI BstEII BstZ17I BtgZI BtsI ClaI CspCI DraI EagI EcoRV FalI FseI FspI FspAI HaeII HindIII HpaI KasI KpnI MfeI MluI MmeI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI PacI PmeI PmlI PpiI PsiI PsrI PvuI RsrII SacII SalI ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII XbaI XmaI ZraI