################################## # NO CUT ENZYMES ( 133 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BalI BbrPI BcgI BciVI BclI BfaI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaWI BseAI BsiEI BsiWI BsmBI BsmFI BsmI BspDI BspEI BspMI BsrI BssSI Bst1107I BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KpnI MaeI MaeII MfeI MluI MluNI MroI MscI MseI MunI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RsrII SalI SapI SbfI ScaI SexAI SfuI SnaBI SpeI SphI Sse8387I SspI StuI SwaI TaqI TfiI Tru9I TspRI Tth111I TthHB8I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AfaI 652 GTAC GT^AC AspHI 237 GAGCTC G[AT]GC[AT]^C AvaII 453 GGTCC G^G[AT]CC BbeI 43 GGCGCC GGCGC^C BbsI 530 GAAGAC GAAGAC(2/6) BbvCI 492 CCTCAGC CCTCAGC(-5/-2) BpuAI 530 GAAGAC GAAGAC(2/6) BsaBI 367 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 331 GAGACC GGTCTC(1/5) BseRI 181 GAGGAG GAGGAG(10/8) BsgI 51 GTGCAG GTGCAG(16/14) BsiHKAI 237 GAGCTC G[AT]GC[AT]^C BsmAI 331 GAGAC GTCTC(1/5) Bsp1407I 651 TGTACA T^GTACA BspHI 375 TCATGA T^CATGA BsrDI 56 GCAATG GCAATG(2/0) BsrGI 651 TGTACA T^GTACA BssHII 137 GCGCGC G^CGCGC BstAPI 264 GCAAGGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstF5I 231 CATCC GGATG(2/0) EaeI 299 CGGCCA [CT]^GGCC[GA] EarI 724 GAAGAG CTCTTC(1/4) FokI 231 CATCC GGATG(9/13) HgaI 279 GACGC GACGC(5/10) KasI 43 GGCGCC G^GCGCC Ksp632I 724 GAAGAG CTCTTC(1/4) MaeIII 676 GTGAC ^GT[ACGT]AC MamI 367 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NaeI 102 GCCGGC GCC^GGC NarI 43 GGCGCC GG^CGCC NgoMIV 102 GCCGGC G^CCGGC PflMI 439 CCATTTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG RcaI 375 TCATGA T^CATGA RsaI 652 GTAC GT^AC SacI 237 GAGCTC GAGCT^C SfiI 92 GGCCCAGCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 43 GGCGCC GGC^GCC SgrAI 23 CACCGGCG C[GA]^CCGG[CT]G SmaI 88 CCCGGG CCC^GGG SmlI 274 CTCAAG C^T[CT][GA]AG SspBI 651 TGTACA T^GTACA Tsp45I 676 GTGAC ^GT[GC]AC Tsp509I 564 AATT ^AATT Van91I 439 CCATTTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 680 CCAGCACAATGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 88 CCCGGG C^CCGGG XmaI 88 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AcyI 43 GGCGCC G[GA]^CG[CT]C 279 GACGCC AflIII 407 ACATGT A^C[GA][CT]GT 629 ACATGT AluI 194 AGCT AG^CT 238 AGCT ApaI 9 GGGCCC GGGCC^C 91 GGGCCC BamHI 75 GGATCC G^GATCC 708 GGATCC BanI 21 GGCACC G^G[CT][GA]CC 43 GGCGCC BglI 93 GCCCAGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 114 GCCCCCGCGGC BpmI 285 CTGGAG CTGGAG(16/14) 599 CTGGAG BsaHI 43 GGCGCC G[GA]^CG[CT]C 279 GACGCC BspLU11I 407 ACATGT A^CATGT 629 ACATGT BsrBI 70 GAGCGG CCGCTC(-3/-3) 166 GAGCGG BsrFI 24 ACCGGC [GA]^CCGG[CT] 102 GCCGGC BstYI 75 GGATCC [GA]^GATC[CT] 708 GGATCC Cfr10I 24 ACCGGC [GA]^CCGG[CT] 102 GCCGGC DraII 8 AGGGCCC [GA]G^G[ACGT]CC[CT] 9 GGGCCCT EcoO109I 8 AGGGCCC [GA]G^G[ACGT]CC[CT] 9 GGGCCCT EcoT14I 317 CCAAGG C^C[AT][AT]GG 486 CCTTGG HaeII 43 GGCGCC [GA]GCGC^[CT] 548 AGCGCC Hin1I 43 GGCGCC G[GA]^CG[CT]C 279 GACGCC HphI 387 GGTGA GGTGA(8/7) 314 TCACC MflI 75 GGATCC [GA]^GATC[CT] 708 GGATCC NspI 407 ACATGT [GA]CATG^[CT] 629 ACATGT SfaNI 355 GATGC GCATC(5/9) 426 GCATC SfcI 522 CTATAG C^T[GA][CT]AG 569 CTGTAG StyI 317 CCAAGG C^C[AT][AT]GG 486 CCTTGG XhoII 75 GGATCC [GA]^GATC[CT] 708 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 52 enzymes) # ######################################### AccII AciI AlwI AvaI BanII BbvI BcnI BmyI BsaJI BsiYI BslI BsoBI Bsp1286I BssKI BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DsaI EcoRII FauI Fnu4HI HaeIII HapII HhaI HinP1I HinfI HpaII ItaI KspI MboI MboII MnlI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI SacII Sau3AI Sau96I ScrFI TseI