(Circular) (Six-base) MAPSORT of: pFN21AE5225.seq Check: 8585 from: 1 to: 5455 pFN21AE5225 5455 bp With 182 enzymes: SgfI * January 30, 2014 17:15 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3265 278 Size: 53 83 186 936 1729 2468 Fragments arranged by size: 2468 1729 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2798 1937 Size: 861 4594 Acc65I G'GTAC_C Cuts at: 2776 2776 Size: 5455 AclI AA'CG_TT Cuts at: 1563 3582 3955 1563 Size: 2019 373 3063 Fragments arranged by size: 3063 2019 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3518 4902 5170 1632 Size: 1886 1384 268 1917 Fragments arranged by size: 1917 1886 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5258 AflIII A'CryG_T Cuts at: 1203 2412 2634 4354 5331 1203 Size: 1209 222 1720 977 1327 Fragments arranged by size: 1720 1327 1209 977 222 AgeI A'CCGG_T Cuts at: 4286 4286 Size: 5455 AhdI GACnn_n'nnGTC Cuts at: 4184 4184 Size: 5455 AleI CACnn'nnGTG Cuts at: 1166 2635 1166 Size: 1469 3986 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5455 AlwNI CAG_nnn'CTG Cuts at: 3198 4770 3198 Size: 1572 3883 ApaI G_GGCC'C Cuts at: 2018 2100 2018 Size: 82 5373 ApaLI G'TGCA_C Cuts at: 3512 4668 3512 Size: 1156 4299 ApoI r'AATT_y Cuts at: 1493 2764 2990 3142 5251 1493 Size: 1271 226 152 2109 1697 Fragments arranged by size: 2109 1697 1271 226 152 AseI AT'TA_AT Cuts at: 160 1032 4009 160 Size: 872 2977 1606 Fragments arranged by size: 2977 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5455 AvaI C'yCGr_G Cuts at: 1870 1943 2072 2093 2129 2724 2780 1870 Size: 73 129 21 36 595 56 4545 Fragments arranged by size: 4545 595 129 73 56 36 21 BamHI G'GATC_C Cuts at: 2080 2713 2785 3152 2080 Size: 633 72 367 4383 Fragments arranged by size: 4383 633 367 72 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2026 2048 2776 4231 618 Size: 325 217 627 239 22 728 1455 1842 Fragments arranged by size: 1842 1455 728 627 325 239 217 22 BanII G_rGCy'C Cuts at: 1391 2018 2100 2246 2774 1391 Size: 627 82 146 528 4072 Fragments arranged by size: 4072 627 528 146 82 BbeI G_GCGC'C Cuts at: 2052 2052 Size: 5455 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2542 928 Size: 971 643 3841 Fragments arranged by size: 3841 971 643 BbvCI CC'TCA_GC Cuts at: 2498 2498 Size: 5455 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3646 3680 1903 Size: 34 1709 34 3678 Fragments arranged by size: 3678 1709 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3347 4557 3347 Size: 1210 4245 BclI T'GATC_A Cuts at: 1548 1548 Size: 5455 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2104 2125 4066 Size: 107 122 71 631 605 432 21 1941 Cuts at: 4066 136 Size: 1525 Fragments arranged by size: 1941 1525 631 605 432 122 107 71 21 BglII A'GATC_T Cuts at: 5450 5450 Size: 5455 BlpI GC'TnA_GC Cuts at: 2872 2872 Size: 5455 Bme1580I G_kGCm'C Cuts at: 1790 2018 2029 2100 3516 4672 1790 Size: 228 11 71 1416 1156 2573 Fragments arranged by size: 2573 1416 1156 228 71 11 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5455 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3186 4144 442 Size: 416 53 83 399 24 1769 958 1753 Fragments arranged by size: 1769 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5455 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2359 2359 Size: 5455 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2311 2625 4115 1239 Size: 1072 314 1490 2579 Fragments arranged by size: 2579 1490 1072 314 Bpu10I CC'TnA_GC Cuts at: 2240 2498 2240 Size: 258 5197 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2264 3580 4445 4743 2264 Size: 1316 865 298 2976 Fragments arranged by size: 2976 1316 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2330 4118 882 Size: 617 831 1788 2219 Fragments arranged by size: 2219 1788 831 617 BsaAI yAC'GTr Cuts at: 493 1204 3246 493 Size: 711 2042 2702 Fragments arranged by size: 2702 2042 711 BsaBI GATnn'nnATC Cuts at: 2376 5449 2376 Size: 3073 2382 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2049 2285 3262 3644 Size: 53 83 186 936 516 236 977 382 Cuts at: 3644 4279 275 Size: 635 1451 Fragments arranged by size: 1451 977 936 635 516 382 236 186 83 53 BsaWI w'CCGG_w Cuts at: 3888 4286 4560 4707 5118 3888 Size: 398 274 147 411 4225 Fragments arranged by size: 4225 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2598 2628 1178 Size: 30 165 30 1195 30 4005 Fragments arranged by size: 4005 1195 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2201 1186 Size: 1015 4440 BseYI C'CCAG_C Cuts at: 2099 2248 2262 4658 5154 2099 Size: 149 14 2396 496 2400 Fragments arranged by size: 2400 2396 496 149 14 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2077 1163 Size: 573 341 4541 Fragments arranged by size: 4541 573 341 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2905 3666 4694 1540 Size: 275 186 904 761 1028 2301 Fragments arranged by size: 2301 1028 904 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2246 3516 3601 4672 2246 Size: 1270 85 1071 3029 Fragments arranged by size: 3029 1270 1071 85 BsmI GAATG_Cn' Cuts at: 2966 3059 2966 Size: 93 5362 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2018 2029 2100 2246 2774 3516 3601 Size: 399 228 11 71 146 528 742 85 Cuts at: 3601 4672 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 528 399 228 146 85 71 11 BspEI T'CCGG_A Cuts at: 5118 5118 Size: 5455 BspHI T'CATG_A Cuts at: 2380 3344 5074 2380 Size: 964 1730 2761 Fragments arranged by size: 2761 1730 964 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2810 844 Size: 1966 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2077 2173 3342 1394 Size: 429 254 96 1169 3507 Fragments arranged by size: 3507 1169 429 254 96 BsrDI GCAATG_nn' Cuts at: 65 1248 2068 3950 4124 65 Size: 1183 820 1882 174 1396 Fragments arranged by size: 1882 1396 1183 820 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2029 2107 4099 4286 1700 Size: 110 219 78 1992 187 2869 Fragments arranged by size: 2869 1992 219 187 110 78 BsrGI T'GTAC_A Cuts at: 96 2656 96 Size: 2560 2895 BssHII G'CGCG_C Cuts at: 1925 2142 1925 Size: 217 5238 BssSI C'ACGA_G Cuts at: 3515 4527 3515 Size: 1012 4443 BstAPI GCAn_nnn'nTGC Cuts at: 794 2275 794 Size: 1481 3974 BstBI TT'CG_AA Cuts at: 4259 4259 Size: 5455 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5455 BstYI r'GATC_y Cuts at: 1972 2080 2713 2785 3152 3537 3554 5006 5092 Size: 108 633 72 367 385 17 1452 86 Cuts at: 5092 5104 5450 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 633 385 367 346 108 86 72 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2042 2087 2123 2151 513 Size: 552 939 38 45 36 28 3817 Fragments arranged by size: 3817 939 552 45 38 36 28 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4716 Fragments arranged by size: 4716 683 56 BtsI GCAGTG_nn' Cuts at: 2971 3765 3785 2971 Size: 794 20 4641 Fragments arranged by size: 4641 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5455 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5420 DraI TTT'AAA Cuts at: 2759 2759 Size: 5455 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4462 809 Size: 507 3146 1802 Fragments arranged by size: 3146 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2304 2902 3791 Size: 54 1425 50 275 18 474 598 889 Cuts at: 3791 5420 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 598 474 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2902 1537 Size: 275 1090 4090 Fragments arranged by size: 4090 1090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2724 3385 1353 Size: 1371 661 3423 Fragments arranged by size: 3423 1371 661 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2048 2073 2126 4042 4416 4562 Size: 1064 138 459 25 53 1916 374 146 Cuts at: 4562 387 Size: 1280 Fragments arranged by size: 1916 1280 1064 459 374 146 138 53 25 EcoICRI GAG'CTC Cuts at: 2244 2244 Size: 5455 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2311 2625 3518 4115 4902 5170 1239 Size: 393 679 314 893 597 787 268 1524 Fragments arranged by size: 1524 893 787 679 597 393 314 268 EcoO109I rG'GnC_Cy Cuts at: 2014 2015 2014 Size: 1 5454 EcoRI G'AATT_C Cuts at: 2764 3142 2764 Size: 378 5077 FspI TGC'GCA Cuts at: 3961 3961 Size: 5455 HaeII r_GCGC'y Cuts at: 1678 2052 2557 4602 1678 Size: 374 505 2045 2531 Fragments arranged by size: 2531 2045 505 374 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4096 4128 4170 4202 1373 Size: 32 135 32 2524 32 42 32 2626 Fragments arranged by size: 2626 2524 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2799 3045 669 Size: 988 281 861 246 3079 Fragments arranged by size: 3079 988 861 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5455 HpaI GTT'AAC Cuts at: 3045 3045 Size: 5455 KasI G'GCGC_C Cuts at: 2048 2048 Size: 5455 KpnI G_GTAC'C Cuts at: 2780 2780 Size: 5455 MfeI C'AATT_G Cuts at: 3054 3054 Size: 5455 MluI A'CGCG_T Cuts at: 5331 5331 Size: 5455 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2431 4569 4753 1305 Size: 1126 2138 184 2007 Fragments arranged by size: 2138 2007 1126 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3633 Fragments arranged by size: 3633 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2411 2635 2639 3413 3772 3931 518 Size: 648 1245 224 4 774 359 159 2042 Fragments arranged by size: 2042 1245 774 648 359 224 159 4 MspA1I CmG'CkG Cuts at: 1606 2044 2089 2125 2153 2266 2871 3548 4696 Size: 438 45 36 28 113 605 677 1148 Cuts at: 4696 4941 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 605 438 245 113 45 36 28 NaeI GCC'GGC Cuts at: 2109 2109 Size: 5455 NarI GG'CG_CC Cuts at: 2049 2049 Size: 5455 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3964 Fragments arranged by size: 3964 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5455 NgoMIV G'CCGG_C Cuts at: 2107 2107 Size: 5455 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5455 NotI GC'GGCC_GC Cuts at: 2902 2902 Size: 5455 NspI r_CATG'y Cuts at: 1132 1229 2416 2638 2813 4358 1132 Size: 97 1187 222 175 1545 2229 Fragments arranged by size: 2229 1545 1187 222 175 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5455 PciI A'CATG_T Cuts at: 2412 2634 4354 2412 Size: 222 1720 3513 Fragments arranged by size: 3513 1720 222 PflMI CCAn_nnn'nTGG Cuts at: 1108 2450 1108 Size: 1342 4113 PfoI T'CCnGG_A Cuts at: 2706 2706 Size: 5455 PmeI GTTT'AAAC Cuts at: 2759 2759 Size: 5455 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3524 3556 5037 5069 3524 Size: 32 1481 32 3910 Fragments arranged by size: 3910 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5455 PsiI TTA'TAA Cuts at: 3025 3025 Size: 5455 PspOMI G'GGCC_C Cuts at: 2014 2096 2014 Size: 82 5373 PstI C_TGCA'G Cuts at: 830 2807 830 Size: 1977 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5455 SacI G_AGCT'C Cuts at: 2246 2246 Size: 5455 SacII CC_GC'GG Cuts at: 2045 2090 2126 2154 2045 Size: 45 36 28 5346 Fragments arranged by size: 5346 45 36 28 SalI G'TCGA_C Cuts at: 1936 2797 1936 Size: 861 4594 SbfI CC_TGCA'GG Cuts at: 2807 2807 Size: 5455 ScaI AGT'ACT Cuts at: 3703 5415 3703 Size: 1712 3743 SfcI C'TryA_G Cuts at: 826 1045 2527 2574 2803 3938 4619 4810 826 Size: 219 1482 47 229 1135 681 191 1471 Fragments arranged by size: 1482 1471 1135 681 229 219 191 47 SfiI GGCCn_nnn'nGGCC Cuts at: 2104 2104 Size: 5455 SfoI GGC'GCC Cuts at: 2050 2050 Size: 5455 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5455 SgrAI Cr'CCGG_yG Cuts at: 1700 2029 1700 Size: 329 5126 SmaI CCC'GGG Cuts at: 1872 2095 2782 1872 Size: 223 687 4545 Fragments arranged by size: 4545 687 223 SmlI C'TyrA_G Cuts at: 820 1017 1943 2279 3559 4460 4722 820 Size: 197 926 336 1280 901 262 1553 Fragments arranged by size: 1553 1280 926 901 336 262 197 SnaBI TAC'GTA Cuts at: 493 3246 493 Size: 2753 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5455 SphI G_CATG'C Cuts at: 1132 2813 1132 Size: 1681 3774 SrfI GCCC'GGGC Cuts at: 2095 2095 Size: 5455 SspI AAT'ATT Cuts at: 5 52 3379 5 Size: 47 3327 2081 Fragments arranged by size: 3327 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5455 StyI C'CwwG_G Cuts at: 513 1065 2004 2322 2491 2894 513 Size: 552 939 318 169 403 3074 Fragments arranged by size: 3074 939 552 403 318 169 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2687 3482 3499 3652 3837 4282 325 Size: 1255 1107 795 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1107 795 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2656 3701 5413 Size: 274 80 33 51 1443 679 1045 1712 Cuts at: 5413 96 Size: 138 Fragments arranged by size: 1712 1443 1045 679 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5415 5447 5415 Size: 32 5423 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5455 XbaI T'CTAG_A Cuts at: 2791 2791 Size: 5455 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2692 2692 Size: 5455 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5455 XmaI C'CCGG_G Cuts at: 1870 2093 2780 1870 Size: 223 687 4545 Fragments arranged by size: 4545 687 223 XmnI GAAnn'nnTTC Cuts at: 3584 3584 Size: 5455 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3263 276 Size: 53 83 186 936 1729 2468 Fragments arranged by size: 2468 1729 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SacI SacII SalI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PpuMI PspXI PsrI PvuII RsrII SanDI SapI SexAI SwaI