(Linear) (Six-base) MAPSORT of: KIEE5225.seq Check: 3109 from: 1 to: 750 KIEE5225.orf With 182 enzymes: SgfI * January 29, 2014 11:51 .. AflIII A'CryG_T Cuts at: 0 407 629 750 Size: 407 222 121 Fragments arranged by size: 407 222 121 AleI CACnn'nnGTG Cuts at: 0 630 750 Size: 630 120 ApaI G_GGCC'C Cuts at: 0 13 95 750 Size: 13 82 655 Fragments arranged by size: 655 82 13 AvaI C'yCGr_G Cuts at: 0 67 88 124 719 750 Size: 67 21 36 595 31 Fragments arranged by size: 595 67 36 31 21 BamHI G'GATC_C Cuts at: 0 75 708 750 Size: 75 633 42 Fragments arranged by size: 633 75 42 BanI G'GyrC_C Cuts at: 0 21 43 750 Size: 21 22 707 Fragments arranged by size: 707 22 21 BanII G_rGCy'C Cuts at: 0 13 95 241 750 Size: 13 82 146 509 Fragments arranged by size: 509 146 82 13 BbeI G_GCGC'C Cuts at: 0 47 750 Size: 47 703 BbsI GAAGACnn'nnnn_ Cuts at: 0 537 750 Size: 537 213 BbvCI CC'TCA_GC Cuts at: 0 493 750 Size: 493 257 BglI GCCn_nnn'nGGC Cuts at: 0 99 120 750 Size: 99 21 630 Fragments arranged by size: 630 99 21 Bme1580I G_kGCm'C Cuts at: 0 13 24 95 750 Size: 13 11 71 655 Fragments arranged by size: 655 71 13 11 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 354 750 Size: 354 396 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 306 620 750 Size: 306 314 130 Fragments arranged by size: 314 306 130 Bpu10I CC'TnA_GC Cuts at: 0 235 493 750 Size: 235 258 257 Fragments arranged by size: 258 257 235 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 259 750 Size: 259 491 BsaI GGTCTCn'nnnn_ Cuts at: 0 325 750 Size: 325 425 BsaBI GATnn'nnATC Cuts at: 0 371 750 Size: 371 379 BsaHI Gr'CG_yC Cuts at: 0 44 280 750 Size: 44 236 470 Fragments arranged by size: 470 236 44 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 593 623 750 Size: 593 30 127 Fragments arranged by size: 593 127 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 196 750 Size: 196 554 BseYI C'CCAG_C Cuts at: 0 94 243 257 750 Size: 94 149 14 493 Fragments arranged by size: 493 149 94 14 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 72 750 Size: 72 678 BsiHKAI G_wGCw'C Cuts at: 0 241 750 Size: 241 509 Bsp1286I G_dGCh'C Cuts at: 0 13 24 95 241 750 Size: 13 11 71 146 509 Fragments arranged by size: 509 146 71 13 11 BspHI T'CATG_A Cuts at: 0 375 750 Size: 375 375 BsrBI CCG'CTC Cuts at: 0 72 168 750 Size: 72 96 582 Fragments arranged by size: 582 96 72 BsrDI GCAATG_nn' Cuts at: 0 63 750 Size: 63 687 BsrFI r'CCGG_y Cuts at: 0 24 102 750 Size: 24 78 648 Fragments arranged by size: 648 78 24 BsrGI T'GTAC_A Cuts at: 0 651 750 Size: 651 99 BssHII G'CGCG_C Cuts at: 0 137 750 Size: 137 613 BstAPI GCAn_nnn'nTGC Cuts at: 0 270 750 Size: 270 480 BstYI r'GATC_y Cuts at: 0 75 708 750 Size: 75 633 42 Fragments arranged by size: 633 75 42 BtgI C'CryG_G Cuts at: 0 37 82 118 146 750 Size: 37 45 36 28 604 Fragments arranged by size: 604 45 37 36 28 EaeI y'GGCC_r Cuts at: 0 299 750 Size: 299 451 EarI CTCTTCn'nnn_ Cuts at: 0 719 750 Size: 719 31 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 43 68 121 750 Size: 43 25 53 629 Fragments arranged by size: 629 53 43 25 EcoICRI GAG'CTC Cuts at: 0 239 750 Size: 239 511 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 306 620 750 Size: 306 314 130 Fragments arranged by size: 314 306 130 EcoO109I rG'GnC_Cy Cuts at: 0 9 10 750 Size: 9 1 740 Fragments arranged by size: 740 9 1 HaeII r_GCGC'y Cuts at: 0 47 552 750 Size: 47 505 198 Fragments arranged by size: 505 198 47 KasI G'GCGC_C Cuts at: 0 43 750 Size: 43 707 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 426 750 Size: 426 324 MslI CAynn'nnrTG Cuts at: 0 406 630 634 750 Size: 406 224 4 116 Fragments arranged by size: 406 224 116 4 MspA1I CmG'CkG Cuts at: 0 39 84 120 148 261 750 Size: 39 45 36 28 113 489 Fragments arranged by size: 489 113 45 39 36 28 NaeI GCC'GGC Cuts at: 0 104 750 Size: 104 646 NarI GG'CG_CC Cuts at: 0 44 750 Size: 44 706 NgoMIV G'CCGG_C Cuts at: 0 102 750 Size: 102 648 NspI r_CATG'y Cuts at: 0 411 633 750 Size: 411 222 117 Fragments arranged by size: 411 222 117 PciI A'CATG_T Cuts at: 0 407 629 750 Size: 407 222 121 Fragments arranged by size: 407 222 121 PflMI CCAn_nnn'nTGG Cuts at: 0 445 750 Size: 445 305 PfoI T'CCnGG_A Cuts at: 0 701 750 Size: 701 49 PspOMI G'GGCC_C Cuts at: 0 9 91 750 Size: 9 82 659 Fragments arranged by size: 659 82 9 SacI G_AGCT'C Cuts at: 0 241 750 Size: 241 509 SacII CC_GC'GG Cuts at: 0 40 85 121 149 750 Size: 40 45 36 28 601 Fragments arranged by size: 601 45 40 36 28 SfcI C'TryA_G Cuts at: 0 522 569 750 Size: 522 47 181 Fragments arranged by size: 522 181 47 SfiI GGCCn_nnn'nGGCC Cuts at: 0 99 750 Size: 99 651 SfoI GGC'GCC Cuts at: 0 45 750 Size: 45 705 SgrAI Cr'CCGG_yG Cuts at: 0 24 750 Size: 24 726 SmaI CCC'GGG Cuts at: 0 90 750 Size: 90 660 SmlI C'TyrA_G Cuts at: 0 274 750 Size: 274 476 SrfI GCCC'GGGC Cuts at: 0 90 750 Size: 90 660 StyI C'CwwG_G Cuts at: 0 317 486 750 Size: 317 169 264 Fragments arranged by size: 317 264 169 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 682 750 Size: 682 68 TatI w'GTAC_w Cuts at: 0 651 750 Size: 651 99 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 687 750 Size: 687 63 XmaI C'CCGG_G Cuts at: 0 88 750 Size: 88 662 Enzymes that do cut: AflIII AleI ApaI AvaI BamHI BanI BanII BbeI BbsI BbvCI BglI Bme1580I BplI BpmI Bpu10I BpuEI BsaI BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspHI BsrBI BsrDI BsrFI BsrGI BssHII BstAPI BstYI BtgI EaeI EarI EciI EcoICRI Eco57MI EcoO109I HaeII KasI MmeI MslI MspA1I NaeI NarI NgoMIV NspI PciI PflMI PfoI PspOMI SacI SacII SfcI SfiI SfoI SgrAI SmaI SmlI SrfI StyI TaqII TatI XcmI XmaI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AgeI AhdI AlfI AloI AlwNI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BcgI BciVI BclI BfrBI BglII BlpI BmgBI BmrI BmtI BsaAI BsaWI BsiEI BsiWI BsmI BsmBI BspEI BspMI BssSI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII DrdI EagI EcoNI EcoRI EcoRV FalI FseI FspI FspAI Hin4I HincII HindIII HpaI KpnI MfeI MluI MscI NcoI NdeI NheI NotI NruI NsiI PacI PasI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PstI PvuI PvuII RsrII SalI SanDI SapI SbfI ScaI SexAI SgfI SnaBI SpeI SphI SspI StuI SwaI TstI Tth111I XbaI XhoI XmnI ZraI