################################## # NO CUT ENZYMES ( 179 enzymes) # ################################## AatII AccI AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI AluI AlwNI AocI Aor51HI ApaI ApoI AscI AseI AsnI Asp700I AspEI AspI AvaI AvaII AviII AvrII BalI BamHI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DdeI DraI DraIII DrdI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HphI KasI Ksp632I KspI MaeII MamI MfeI MluI MluNI MroI MscI MseI MslI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfaNI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tru9I Tsp45I Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### Acc65I 278 GGTACC G^GTACC AccII 14 CGCG CG^CG AfaI 279 GTAC GT^AC Alw44I 147 GTGCAC G^TGCAC AlwI 198 GATCC GGATC(4/5) ApaLI 147 GTGCAC G^TGCAC Asp718I 278 GGTACC G^GTACC BclI 62 TGATCA T^GATCA BfaI 37 CTAG C^TAG BglI 179 GCCACCCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpmI 258 CTCCAG CTGGAG(16/14) BsmAI 19 GTCTC GTCTC(1/5) BsrBI 79 GAGCGG CCGCTC(-3/-3) BsrI 90 CCAGT ACTGG(1/-1) BstNI 260 CCAGG CC^[AT]GG BstUI 14 CGCG CG^CG BstYI 197 AGATCC [GA]^GATC[CT] Cfr13I 71 GGGCC G^G[ACGT]CC DraII 70 GGGGCCT [GA]G^G[ACGT]CC[CT] DsaI 290 CCATGG C^C[GA][CT]GG EcoNI 203 CCTTCTTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 70 GGGGCCT [GA]G^G[ACGT]CC[CT] EcoRII 260 CCAGG ^CC[AT]GG EcoT14I 290 CCATGG C^C[AT][AT]GG FbaI 62 TGATCA T^GATCA HaeIII 72 GGCC GG^CC HinfI 17 GAGTC G^A[ACGT]TC KpnI 278 GGTACC GGTAC^C MaeI 37 CTAG C^TAG MaeIII 40 GTAAC ^GT[ACGT]AC MflI 197 AGATCC [GA]^GATC[CT] MvaI 260 CCAGG CC^[AT]GG MvnI 14 CGCG CG^CG NcoI 290 CCATGG C^CATGG PleI 17 GAGTC GAGTC(4/5) RsaI 279 GTAC GT^AC Sau96I 71 GGGCC G^G[ACGT]CC StyI 290 CCATGG C^C[AT][AT]GG TaqI 132 TCGA T^CGA Tsp509I 310 AATT ^AATT TspRI 91 CAGTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 132 TCGA T^CGA XhoII 197 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AspHI 147 GTGCAC G[AT]GC[AT]^C 255 GTGCTC BcnI 184 CCCGG CC^[GC]GG 267 CCCGG BmyI 147 GTGCAC G[AGT]GC[ACT]^C 255 GTGCTC BsaJI 183 CCCCGG C^C[ACGT][ACGT]GG 290 CCATGG BseRI 31 CTCCTC GAGGAG(10/8) 172 GAGGAG BsiHKAI 147 GTGCAC G[AT]GC[AT]^C 255 GTGCTC Bsp1286I 147 GTGCAC G[AGT]GC[ACT]^C 255 GTGCTC BstF5I 84 GGATG GGATG(2/0) 143 GGATG DpnII 63 GATC ^GATC 198 GATC FokI 84 GGATG GGATG(9/13) 143 GGATG HapII 185 CCGG C^CGG 268 CCGG HpaII 185 CCGG C^CGG 268 CCGG MboI 63 GATC ^GATC 198 GATC MboII 300 GAAGA GAAGA(8/7) 206 TCTTC MspA1I 154 CCGCTG C[AC]G^C[GT]G 282 CCGCTG MspI 185 CCGG C^CGG 268 CCGG NciI 184 CCCGG CC^[GC]GG 267 CCCGG NdeII 63 GATC ^GATC 198 GATC NlaIII 27 CATG CATG^ 291 CATG Sau3AI 63 GATC ^GATC 198 GATC ######################################### # 3 OR MORE CUT ENZYMES ( 16 enzymes) # ######################################### AciI BanI BbvI BsiYI BslI BspMI BssKI Cac8I FauI Fnu4HI ItaI MnlI MwoI NlaIV ScrFI TseI