################################## # NO CUT ENZYMES ( 156 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AflII AflIII AhdI Aor51HI ApaI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BalI BamHI BanII BbeI BbrPI BbvCI BciVI BclI BfaI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BseAI BsiEI BsiWI BsmAI BsmBI BsmI BspEI BspHI BspLU11I BspMI BsrBI BsrI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I CelII CfoI CpoI DraI DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRI EcoT22I FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KpnI KspI MaeI MaeII MaeIII MamI MfeI MflI MluI MluNI MroI MscI MspA1I MunI MvnI NaeI NarI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfaNI SfcI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspI StuI SwaI Tsp45I TspRI Tth111I XbaI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### AfaI 393 GTAC GT^AC AgeI 212 ACCGGT A^CCGGT Alw44I 328 GTGCAC G^TGCAC AlwNI 704 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 7 CCTAAGG CC^T[ACGT]AGG ApaLI 328 GTGCAC G^TGCAC AspHI 328 GTGCAC G[AT]GC[AT]^C BanI 3 GGCACC G^G[CT][GA]CC BcgI 456 CGACCTGATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglI 717 GCCACCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaWI 212 ACCGGT [AT]^CCGG[AT] BsiHKAI 328 GTGCAC G[AT]GC[AT]^C BsmFI 681 GGGAC GGGAC(10/14) Bsp1407I 392 TGTACA T^GTACA BspDI 580 ATCGAT AT^CGAT BsrDI 301 GCAATG GCAATG(2/0) BsrFI 212 ACCGGT [GA]^CCGG[CT] BsrGI 392 TGTACA T^GTACA BssSI 189 CACGAG CACGAG(-5/-1) BstF5I 426 CATCC GGATG(2/0) Bsu36I 7 CCTAAGG CC^T[ACGT]AGG Cfr10I 212 ACCGGT [GA]^CCGG[CT] ClaI 580 ATCGAT AT^CGAT DraII 663 AGGACCC [GA]G^G[ACGT]CC[CT] DraIII 178 CACGAAGTG CAC[ACGT][ACGT][ACGT]^GTG EarI 448 CTCTTC CTCTTC(1/4) Eco81I 7 CCTAAGG CC^T[ACGT]AGG EcoNI 643 CCTCCTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 663 AGGACCC [GA]G^G[ACGT]CC[CT] EcoRV 363 GATATC GAT^ATC EcoT14I 356 CCATGG C^C[AT][AT]GG FauI 691 CCCGC CCCGC(4/6) FokI 426 CATCC GGATG(9/13) Ksp632I 448 CTCTTC CTCTTC(1/4) MseI 223 TTAA T^TAA MslI 351 CATCACCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NcoI 356 CCATGG C^CATGG NdeI 631 CATATG CA^TATG PflMI 703 CCAGAACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 212 ACCGGT A^CCGGT PpuMI 663 AGGACCC [GA]G^G[AT]CC[CT] RsaI 393 GTAC GT^AC SmaI 58 CCCGGG CCC^GGG SmlI 339 CTTGAG C^T[CT][GA]AG SspBI 392 TGTACA T^GTACA StyI 356 CCATGG C^C[AT][AT]GG TaqI 581 TCGA T^CGA Tru9I 223 TTAA T^TAA Tsp509I 221 AATT ^AATT TthHB8I 581 TCGA T^CGA Van91I 703 CCAGAACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 173 CCAAGCACGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 58 CCCGGG C^CCGGG XmaI 58 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AlwI 515 GATCC GGATC(4/5) 546 GATCC AvaI 58 CCCGGG C^[CT]CG[GA]G 70 CCCGAG BbsI 76 GAAGAC GAAGAC(2/6) 590 GTCTTC BcnI 58 CCCGG CC^[GC]GG 59 CCGGG BpmI 647 CTCCAG CTGGAG(16/14) 563 CTGGAG BpuAI 76 GAAGAC GAAGAC(2/6) 590 GTCTTC BseRI 644 CTCCTC GAGGAG(10/8) 192 GAGGAG BsgI 124 GTGCAG GTGCAG(16/14) 166 GTGCAG BsoBI 58 CCCGGG C^[CT]CG[GA]G 70 CCCGAG DdeI 8 CTAAG C^T[ACGT]AG 263 CTGAG DsaI 356 CCATGG C^C[GA][CT]GG 488 CCGTGG HapII 59 CCGG C^CGG 213 CCGG HinfI 273 GATTC G^A[ACGT]TC 522 GAATC HpaII 59 CCGG C^CGG 213 CCGG HphI 323 TCACC GGTGA(8/7) 353 TCACC MspI 59 CCGG C^CGG 213 CCGG NciI 58 CCCGG CC^[GC]GG 59 CCGGG NlaIII 357 CATG CATG^ 519 CATG TfiI 273 GATTC G^A[AT]TC 522 GAATC ######################################### # 3 OR MORE CUT ENZYMES ( 29 enzymes) # ######################################### AciI AluI AvaII BbvI BmyI BsaJI BsiYI BslI Bsp1286I BssKI BstNI Cac8I Cfr13I DpnII EcoRII Fnu4HI HaeIII ItaI MboI MboII MnlI MvaI MwoI NdeII NlaIV Sau3AI Sau96I ScrFI TseI