(Circular) (Six-base) MAPSORT of: pFN21AE3592.seq Check: 547 from: 1 to: 5449 pFN21AE3592 5449 bp With 182 enzymes: SgfI * August 8, 2011 18:07 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3259 278 Size: 53 83 186 936 1723 2468 Fragments arranged by size: 2468 1723 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2792 1937 Size: 855 4594 Acc65I G'GTAC_C Cuts at: 2770 2770 Size: 5449 AclI AA'CG_TT Cuts at: 1563 3576 3949 1563 Size: 2013 373 3063 Fragments arranged by size: 3063 2013 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2362 2596 3512 4896 5164 1632 Size: 730 234 916 1384 268 1917 Fragments arranged by size: 1917 1384 916 730 268 234 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5252 AflIII A'CryG_T Cuts at: 1203 4348 5325 1203 Size: 3145 977 1327 Fragments arranged by size: 3145 1327 977 AgeI A'CCGG_T Cuts at: 2217 4280 2217 Size: 2063 3386 AhdI GACnn_n'nnGTC Cuts at: 4178 4178 Size: 5449 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5449 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5449 AlwNI CAG_nnn'CTG Cuts at: 2714 3192 4764 2714 Size: 478 1572 3399 Fragments arranged by size: 3399 1572 478 ApaLI G'TGCA_C Cuts at: 2333 3506 4662 2333 Size: 1173 1156 3120 Fragments arranged by size: 3120 1173 1156 ApoI r'AATT_y Cuts at: 1493 2758 2984 3136 5245 1493 Size: 1265 226 152 2109 1697 Fragments arranged by size: 2109 1697 1265 226 152 AseI AT'TA_AT Cuts at: 160 1032 4003 160 Size: 872 2971 1606 Fragments arranged by size: 2971 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5449 AvaI C'yCGr_G Cuts at: 1870 1943 2063 2075 2774 1870 Size: 73 120 12 699 4545 Fragments arranged by size: 4545 699 120 73 12 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2303 2336 2303 Size: 33 5416 BamHI G'GATC_C Cuts at: 2779 3146 2779 Size: 367 5082 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2008 2770 4225 618 Size: 325 217 627 221 762 1455 1842 Fragments arranged by size: 1842 1455 762 627 325 221 217 BanII G_rGCy'C Cuts at: 1391 2768 1391 Size: 1377 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2088 2588 928 Size: 971 189 500 3789 Fragments arranged by size: 3789 971 500 189 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2450 2484 3640 3674 1903 Size: 34 513 34 1156 34 3678 Fragments arranged by size: 3678 1156 513 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3341 4551 3341 Size: 1210 4239 BclI T'GATC_A Cuts at: 1548 1548 Size: 5449 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2728 4060 136 Size: 107 122 71 631 605 1056 1332 1525 Fragments arranged by size: 1525 1332 1056 631 605 122 107 71 BglII A'GATC_T Cuts at: 5444 5444 Size: 5449 BlpI GC'TnA_GC Cuts at: 2866 2866 Size: 5449 Bme1580I G_kGCm'C Cuts at: 1790 2195 2265 2337 3510 4666 1790 Size: 405 70 72 1173 1156 2573 Fragments arranged by size: 2573 1173 1156 405 72 70 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5449 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3180 4138 442 Size: 416 53 83 399 24 1763 958 1753 Fragments arranged by size: 1763 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5449 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2589 2637 4109 1239 Size: 1350 48 1472 2579 Fragments arranged by size: 2579 1472 1350 48 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2365 3574 4439 4737 2365 Size: 1209 865 298 3077 Fragments arranged by size: 3077 1209 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4112 882 Size: 617 2613 2219 Fragments arranged by size: 2613 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3240 493 Size: 711 2036 2702 Fragments arranged by size: 2702 2036 711 BsaBI GATnn'nnATC Cuts at: 5443 5443 Size: 5449 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3256 3638 4273 275 Size: 53 83 186 936 1723 382 635 1451 Fragments arranged by size: 1723 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2217 3882 4280 4554 4701 5112 2217 Size: 1665 398 274 147 411 2554 Fragments arranged by size: 2554 1665 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2191 2221 2481 2511 1178 Size: 30 165 30 788 30 260 30 4116 Fragments arranged by size: 4116 788 260 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2212 2640 1186 Size: 1026 428 3995 Fragments arranged by size: 3995 1026 428 BseYI C'CCAG_C Cuts at: 2673 4652 5148 2673 Size: 1979 496 2974 Fragments arranged by size: 2974 1979 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2150 2192 1163 Size: 573 414 42 4420 Fragments arranged by size: 4420 573 414 42 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2899 3660 4688 1540 Size: 275 186 898 761 1028 2301 Fragments arranged by size: 2301 1028 898 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2337 3510 3595 4666 2337 Size: 1173 85 1071 3120 Fragments arranged by size: 3120 1173 1071 85 BsmI GAATG_Cn' Cuts at: 2960 3053 2960 Size: 93 5356 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2195 2265 2337 2768 3510 3595 4666 Size: 399 405 70 72 431 742 85 1071 Cuts at: 4666 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 431 405 399 85 72 70 BspEI T'CCGG_A Cuts at: 5112 5112 Size: 5449 BspHI T'CATG_A Cuts at: 3338 5068 3338 Size: 1730 3719 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2804 844 Size: 1960 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3336 1394 Size: 429 1513 3507 Fragments arranged by size: 3507 1513 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2313 3944 4118 65 Size: 1183 1065 1631 174 1396 Fragments arranged by size: 1631 1396 1183 1065 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2217 4093 4280 1700 Size: 110 407 1876 187 2869 Fragments arranged by size: 2869 1876 407 187 110 BsrGI T'GTAC_A Cuts at: 96 2397 96 Size: 2301 3148 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5449 BssSI C'ACGA_G Cuts at: 2194 3509 4521 2194 Size: 1315 1012 3122 Fragments arranged by size: 3122 1315 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5449 BstBI TT'CG_AA Cuts at: 4253 4253 Size: 5449 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5449 BstYI r'GATC_y Cuts at: 1972 2779 3146 3531 3548 5000 5086 5098 5444 Size: 807 367 385 17 1452 86 12 346 Cuts at: 5444 1972 Size: 1977 Fragments arranged by size: 1977 1452 807 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2013 2013 Size: 5449 BtgI C'CryG_G Cuts at: 513 1065 2004 2361 2493 513 Size: 552 939 357 132 3469 Fragments arranged by size: 3469 939 552 357 132 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4710 Fragments arranged by size: 4710 683 56 BtsI GCAGTG_nn' Cuts at: 2965 3759 3779 2965 Size: 794 20 4635 Fragments arranged by size: 4635 794 20 ClaI AT'CG_AT Cuts at: 1554 2586 1554 Size: 1032 4417 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5414 DraI TTT'AAA Cuts at: 2753 2753 Size: 5449 DraIII CAC_nnn'GTG Cuts at: 2188 2188 Size: 5449 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4456 809 Size: 507 3140 1802 Fragments arranged by size: 3140 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2896 3785 5414 Size: 54 1425 50 275 18 1066 889 1629 Cuts at: 5414 8 Size: 43 Fragments arranged by size: 1629 1425 1066 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2896 1537 Size: 275 1084 4090 Fragments arranged by size: 4090 1084 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2459 3379 1353 Size: 1106 920 3423 Fragments arranged by size: 3423 1106 920 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4036 4410 4556 387 Size: 1064 138 2447 374 146 1280 Fragments arranged by size: 2447 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2362 2589 2596 2637 3512 4109 4896 Size: 393 730 227 7 41 875 597 787 Cuts at: 4896 5164 1239 Size: 268 1524 Fragments arranged by size: 1524 875 787 730 597 393 268 227 41 7 EcoNI CCTnn'n_nnAGG Cuts at: 2652 2652 Size: 5449 EcoO109I rG'GnC_Cy Cuts at: 2669 2669 Size: 5449 EcoRI G'AATT_C Cuts at: 2758 3136 2758 Size: 378 5071 EcoRV GAT'ATC Cuts at: 2370 2370 Size: 5449 FspI TGC'GCA Cuts at: 3955 3955 Size: 5449 HaeII r_GCGC'y Cuts at: 1678 4596 1678 Size: 2918 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2191 2223 2481 2513 4090 Size: 32 135 32 619 32 258 32 1577 Cuts at: 4090 4122 4164 4196 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1577 619 258 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2793 3039 669 Size: 988 281 855 246 3079 Fragments arranged by size: 3079 988 855 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5449 HpaI GTT'AAC Cuts at: 3039 3039 Size: 5449 KpnI G_GTAC'C Cuts at: 2774 2774 Size: 5449 MfeI C'AATT_G Cuts at: 3048 3048 Size: 5449 MluI A'CGCG_T Cuts at: 5325 5325 Size: 5449 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4563 4747 1305 Size: 3258 184 2007 Fragments arranged by size: 3258 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3627 Fragments arranged by size: 3627 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2360 3407 3766 3925 518 Size: 648 1194 1047 359 159 2042 Fragments arranged by size: 2042 1194 1047 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2865 3542 4690 4935 1606 Size: 1259 677 1148 245 2120 Fragments arranged by size: 2120 1259 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2361 513 Size: 552 939 357 3601 Fragments arranged by size: 3601 939 552 357 NdeI CA'TA_TG Cuts at: 387 2637 387 Size: 2250 3199 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5449 NotI GC'GGCC_GC Cuts at: 2896 2896 Size: 5449 NspI r_CATG'y Cuts at: 1132 1229 2807 4352 1132 Size: 97 1578 1545 2229 Fragments arranged by size: 2229 1578 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5449 PciI A'CATG_T Cuts at: 4348 4348 Size: 5449 PflMI CCAn_nnn'nTGG Cuts at: 1108 2714 1108 Size: 1606 3843 PfoI T'CCnGG_A Cuts at: 2653 2653 Size: 5449 PmeI GTTT'AAAC Cuts at: 2753 2753 Size: 5449 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3518 3550 5031 5063 3518 Size: 32 1481 32 3904 Fragments arranged by size: 3904 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2669 2669 Size: 5449 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5449 PsiI TTA'TAA Cuts at: 3019 3019 Size: 5449 PstI C_TGCA'G Cuts at: 830 2801 830 Size: 1971 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5449 SalI G'TCGA_C Cuts at: 1936 2791 1936 Size: 855 4594 SbfI CC_TGCA'GG Cuts at: 2801 2801 Size: 5449 ScaI AGT'ACT Cuts at: 3697 5409 3697 Size: 1712 3737 SfcI C'TryA_G Cuts at: 826 1045 2797 3932 4613 4804 826 Size: 219 1752 1135 681 191 1471 Fragments arranged by size: 1752 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5449 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5449 SmaI CCC'GGG Cuts at: 1872 2065 2776 1872 Size: 193 711 4545 Fragments arranged by size: 4545 711 193 SmlI C'TyrA_G Cuts at: 820 1017 1943 2344 3553 4454 4716 820 Size: 197 926 401 1209 901 262 1553 Fragments arranged by size: 1553 1209 926 901 401 262 197 SnaBI TAC'GTA Cuts at: 493 3240 493 Size: 2747 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5449 SphI G_CATG'C Cuts at: 1132 2807 1132 Size: 1675 3774 SspI AAT'ATT Cuts at: 5 52 3373 5 Size: 47 3321 2081 Fragments arranged by size: 3321 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5449 StyI C'CwwG_G Cuts at: 513 1065 2004 2361 2888 513 Size: 552 939 357 527 3074 Fragments arranged by size: 3074 939 552 527 357 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3476 3493 3646 3831 4276 325 Size: 1255 1896 17 153 185 445 1498 Fragments arranged by size: 1896 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2397 3695 5407 Size: 274 80 33 51 1443 420 1298 1712 Cuts at: 5407 96 Size: 138 Fragments arranged by size: 1712 1443 1298 420 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5409 5441 5409 Size: 32 5417 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5449 XbaI T'CTAG_A Cuts at: 2785 2785 Size: 5449 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2185 2185 Size: 5449 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5449 XmaI C'CCGG_G Cuts at: 1870 2063 2774 1870 Size: 193 711 4545 Fragments arranged by size: 4545 711 193 XmnI GAAnn'nnTTC Cuts at: 3578 3578 Size: 5449 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3257 276 Size: 53 83 186 936 1723 2468 Fragments arranged by size: 2468 1723 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I EcoICRI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI