(Circular) (Six-base) MAPSORT of: pFN21AB5867.seq Check: 5833 from: 1 to: 6091 pFN21AB5867 6091 bp With 182 enzymes: SgfI * April 15, 2010 09:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3901 278 Size: 53 83 186 936 2365 2468 Fragments arranged by size: 2468 2365 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2809 3434 1937 Size: 872 625 4594 Fragments arranged by size: 4594 872 625 Acc65I G'GTAC_C Cuts at: 3412 3412 Size: 6091 AclI AA'CG_TT Cuts at: 1563 4218 4591 1563 Size: 2655 373 3063 Fragments arranged by size: 3063 2655 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2502 3288 4154 5538 5806 1632 Size: 870 786 866 1384 268 1917 Fragments arranged by size: 1917 1384 870 866 786 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5894 AflIII A'CryG_T Cuts at: 1203 4990 5967 1203 Size: 3787 977 1327 Fragments arranged by size: 3787 1327 977 AgeI A'CCGG_T Cuts at: 4922 4922 Size: 6091 AhdI GACnn_n'nnGTC Cuts at: 4820 4820 Size: 6091 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6091 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3110 1184 Size: 1926 4165 AlwNI CAG_nnn'CTG Cuts at: 3834 5406 3834 Size: 1572 4519 ApaLI G'TGCA_C Cuts at: 4148 5304 4148 Size: 1156 4935 ApoI r'AATT_y Cuts at: 1493 2519 3230 3400 3626 3778 5887 1493 Size: 1026 711 170 226 152 2109 1697 Fragments arranged by size: 2109 1697 1026 711 226 170 152 AseI AT'TA_AT Cuts at: 160 1032 4645 160 Size: 872 3613 1606 Fragments arranged by size: 3613 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6091 AvaI C'yCGr_G Cuts at: 1870 1943 3416 1870 Size: 73 1473 4545 Fragments arranged by size: 4545 1473 73 BamHI G'GATC_C Cuts at: 3421 3788 3421 Size: 367 5724 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3263 3412 4867 618 Size: 325 217 627 1476 149 1455 1842 Fragments arranged by size: 1842 1476 1455 627 325 217 149 BanII G_rGCy'C Cuts at: 1391 3410 1391 Size: 2019 4072 BbeI G_GCGC'C Cuts at: 3267 3267 Size: 6091 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2102 2562 2844 2949 928 Size: 971 203 460 282 105 4070 Fragments arranged by size: 4070 971 460 282 203 105 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3101 3135 4282 4316 1903 Size: 34 1164 34 1147 34 3678 Fragments arranged by size: 3678 1164 1147 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3983 5193 3983 Size: 1210 4881 BclI T'GATC_A Cuts at: 1548 1548 Size: 6091 BfrBI ATG'CAT Cuts at: 2633 2633 Size: 6091 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4702 136 Size: 107 122 71 631 605 3030 1525 Fragments arranged by size: 3030 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2665 6086 2665 Size: 3421 2670 BlpI GC'TnA_GC Cuts at: 3508 3508 Size: 6091 Bme1580I G_kGCm'C Cuts at: 1790 2434 4152 5308 1790 Size: 644 1718 1156 2573 Fragments arranged by size: 2573 1718 1156 644 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6091 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3822 4780 442 Size: 416 53 83 399 24 2405 958 1753 Fragments arranged by size: 2405 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6091 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2125 4751 1239 Size: 886 2626 2579 Fragments arranged by size: 2626 2579 886 Bpu10I CC'TnA_GC Cuts at: 2028 2184 2028 Size: 156 5935 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4216 5081 5379 4216 Size: 865 298 4928 Fragments arranged by size: 4928 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2754 4754 882 Size: 617 1255 2000 2219 Fragments arranged by size: 2219 2000 1255 617 BsaAI yAC'GTr Cuts at: 493 1204 3355 3882 493 Size: 711 2151 527 2702 Fragments arranged by size: 2702 2151 711 527 BsaBI GATnn'nnATC Cuts at: 2709 6085 2709 Size: 3376 2715 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3264 3898 4280 4915 Size: 53 83 186 936 1731 634 382 635 Cuts at: 4915 275 Size: 1451 Fragments arranged by size: 1731 1451 936 635 634 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4524 4922 5196 5343 5754 4524 Size: 398 274 147 411 4861 Fragments arranged by size: 4861 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5866 Fragments arranged by size: 5866 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2706 1186 Size: 1520 4571 BseYI C'CCAG_C Cuts at: 2647 5294 5790 2647 Size: 2647 496 2948 Fragments arranged by size: 2948 2647 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5518 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3541 4302 5330 1540 Size: 275 186 1540 761 1028 2301 Fragments arranged by size: 2301 1540 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2930 4152 4237 5308 2930 Size: 1222 85 1071 3713 Fragments arranged by size: 3713 1222 1071 85 BsmI GAATG_Cn' Cuts at: 3138 3602 3695 3138 Size: 464 93 5534 Fragments arranged by size: 5534 464 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2434 2930 3410 4152 4237 5308 1391 Size: 399 644 496 480 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 644 496 480 399 85 BspEI T'CCGG_A Cuts at: 5754 5754 Size: 6091 BspHI T'CATG_A Cuts at: 3980 5710 3980 Size: 1730 4361 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3292 3446 844 Size: 2448 154 3489 Fragments arranged by size: 3489 2448 154 BsrBI CCG'CTC Cuts at: 1394 1823 3978 1394 Size: 429 2155 3507 Fragments arranged by size: 3507 2155 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4586 4760 65 Size: 1183 3338 174 1396 Fragments arranged by size: 3338 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4735 4922 1700 Size: 110 2925 187 2869 Fragments arranged by size: 2925 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6091 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6091 BssSI C'ACGA_G Cuts at: 4151 5163 4151 Size: 1012 5079 BstAPI GCAn_nnn'nTGC Cuts at: 794 2021 794 Size: 1227 4864 BstBI TT'CG_AA Cuts at: 4895 4895 Size: 6091 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6091 BstYI r'GATC_y Cuts at: 1972 2665 2704 3116 3340 3421 3788 4173 4190 Size: 693 39 412 224 81 367 385 17 Cuts at: 4190 5642 5728 5740 6086 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 693 412 385 367 346 224 86 81 39 17 12 BstZ17I GTA'TAC Cuts at: 2810 2810 Size: 6091 Bsu36I CC'TnA_GG Cuts at: 2847 2847 Size: 6091 BtgI C'CryG_G Cuts at: 513 1065 2079 2157 2608 513 Size: 552 1014 78 451 3996 Fragments arranged by size: 3996 1014 552 451 78 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5352 Fragments arranged by size: 5352 683 56 BtsI GCAGTG_nn' Cuts at: 2122 2239 3092 3607 4401 4421 2122 Size: 117 853 515 794 20 3792 Fragments arranged by size: 3792 853 794 515 117 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6091 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6056 DraI TTT'AAA Cuts at: 2125 2139 3395 2125 Size: 14 1256 4821 Fragments arranged by size: 4821 1256 14 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5098 809 Size: 507 3782 1802 Fragments arranged by size: 3782 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2071 2236 3349 Size: 54 1425 50 275 18 241 165 1113 Cuts at: 3349 3538 4427 6056 8 Size: 189 889 1629 43 Fragments arranged by size: 1629 1425 1113 889 275 241 189 165 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3538 1537 Size: 275 1726 4090 Fragments arranged by size: 4090 1726 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2182 2292 2319 2466 4021 1353 Size: 829 110 27 147 1555 3423 Fragments arranged by size: 3423 1555 829 147 110 27 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4678 5052 5198 387 Size: 1064 138 3089 374 146 1280 Fragments arranged by size: 3089 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2125 2502 3288 4154 4751 5538 5806 Size: 393 493 377 786 866 597 787 268 Cuts at: 5806 1239 Size: 1524 Fragments arranged by size: 1524 866 787 786 597 493 393 377 268 EcoRI G'AATT_C Cuts at: 2519 3400 3778 2519 Size: 881 378 4832 Fragments arranged by size: 4832 881 378 EcoRV GAT'ATC Cuts at: 2796 2796 Size: 6091 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2293 2991 2293 Size: 698 5393 FspI TGC'GCA Cuts at: 4597 4597 Size: 6091 HaeII r_GCGC'y Cuts at: 1678 2194 3267 5238 1678 Size: 516 1073 1971 2531 Fragments arranged by size: 2531 1971 1073 516 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4732 4764 4806 4838 1373 Size: 32 135 32 3160 32 42 32 2626 Fragments arranged by size: 3160 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3435 3681 669 Size: 988 281 1497 246 3079 Fragments arranged by size: 3079 1497 988 281 246 HindIII A'AGCT_T Cuts at: 748 2602 748 Size: 1854 4237 HpaI GTT'AAC Cuts at: 3681 3681 Size: 6091 KasI G'GCGC_C Cuts at: 3263 3263 Size: 6091 KpnI G_GTAC'C Cuts at: 3416 3416 Size: 6091 MfeI C'AATT_G Cuts at: 3690 3690 Size: 6091 MluI A'CGCG_T Cuts at: 5967 5967 Size: 6091 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2823 5205 5389 1305 Size: 1518 2382 184 2007 Fragments arranged by size: 2382 2007 1518 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2238 3351 10 Size: 54 1425 343 406 1113 2750 Fragments arranged by size: 2750 1425 1113 406 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4049 4408 4567 518 Size: 648 2883 359 159 2042 Fragments arranged by size: 2883 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2651 2765 3507 4184 5332 5577 1606 Size: 1045 114 742 677 1148 245 2120 Fragments arranged by size: 2120 1148 1045 742 677 245 114 NarI GG'CG_CC Cuts at: 3264 3264 Size: 6091 NcoI C'CATG_G Cuts at: 513 1065 2079 2157 513 Size: 552 1014 78 4447 Fragments arranged by size: 4447 1014 552 78 NdeI CA'TA_TG Cuts at: 387 387 Size: 6091 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6091 NotI GC'GGCC_GC Cuts at: 3538 3538 Size: 6091 NsiI A_TGCA'T Cuts at: 2635 2635 Size: 6091 NspI r_CATG'y Cuts at: 1132 1229 2213 3449 4994 1132 Size: 97 984 1236 1545 2229 Fragments arranged by size: 2229 1545 1236 984 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6091 PciI A'CATG_T Cuts at: 4990 4990 Size: 6091 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6091 PmeI GTTT'AAAC Cuts at: 3395 3395 Size: 6091 PmlI CAC'GTG Cuts at: 3355 3355 Size: 6091 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4160 4192 5673 5705 4160 Size: 32 1481 32 4546 Fragments arranged by size: 4546 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6091 PsiI TTA'TAA Cuts at: 3661 3661 Size: 6091 PstI C_TGCA'G Cuts at: 830 2662 3443 830 Size: 1832 781 3478 Fragments arranged by size: 3478 1832 781 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6091 PvuII CAG'CTG Cuts at: 2651 2765 2651 Size: 114 5977 SalI G'TCGA_C Cuts at: 1936 3433 1936 Size: 1497 4594 SbfI CC_TGCA'GG Cuts at: 3443 3443 Size: 6091 ScaI AGT'ACT Cuts at: 4339 6051 4339 Size: 1712 4379 SfcI C'TryA_G Cuts at: 826 1045 2164 2658 2932 3439 4574 5255 5446 Size: 219 1119 494 274 507 1135 681 191 Cuts at: 5446 826 Size: 1471 Fragments arranged by size: 1471 1135 1119 681 507 494 274 219 191 SfoI GGC'GCC Cuts at: 3265 3265 Size: 6091 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6091 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6091 SmaI CCC'GGG Cuts at: 1872 3418 1872 Size: 1546 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4195 5096 5358 820 Size: 197 926 2252 901 262 1553 Fragments arranged by size: 2252 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3882 493 Size: 3389 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6091 SphI G_CATG'C Cuts at: 1132 2213 3449 1132 Size: 1081 1236 3774 Fragments arranged by size: 3774 1236 1081 SspI AAT'ATT Cuts at: 5 52 2143 4015 5 Size: 47 2091 1872 2081 Fragments arranged by size: 2091 2081 1872 47 StuI AGG'CCT Cuts at: 1517 2458 2950 1517 Size: 941 492 4658 Fragments arranged by size: 4658 941 492 StyI C'CwwG_G Cuts at: 513 1065 2079 2157 3530 513 Size: 552 1014 78 1373 3074 Fragments arranged by size: 3074 1373 1014 552 78 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2413 2601 3335 4118 4135 4288 4473 Size: 1255 833 188 734 783 17 153 185 Cuts at: 4473 4918 325 Size: 445 1498 Fragments arranged by size: 1498 1255 833 783 734 445 188 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4337 6049 96 Size: 274 80 33 51 1443 2360 1712 138 Fragments arranged by size: 2360 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2989 3021 6051 6083 2989 Size: 32 3030 32 2997 Fragments arranged by size: 3030 2997 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6091 XbaI T'CTAG_A Cuts at: 3427 3427 Size: 6091 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6091 XmaI C'CCGG_G Cuts at: 1870 3416 1870 Size: 1546 4545 XmnI GAAnn'nnTTC Cuts at: 4220 4220 Size: 6091 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3899 276 Size: 53 83 186 936 2365 2468 Fragments arranged by size: 2468 2365 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BplI BsiWI BsmBI BstXI DraIII EcoICRI EcoNI EcoO109I FseI FspAI NaeI NgoMIV NruI PacI PfoI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI