################################## # NO CUT ENZYMES ( 64 enzymes) # ################################## AatII Acc65I AclI AflII AgeI Aor51HI AscI AseI AsnI Asp718I AspI AvrII BamHI BcgI BciVI BclI BfrI BlnI BsaBI BsiWI BspDI BspHI Bst1107I BstBI BstZ17I ClaI CpoI DraI Eco47III EcoRV EcoT22I FbaI FseI HpaI KpnI MamI MfeI MluI MunI NcoI NdeI NruI NsiI NspV PacI PflFI PinAI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SalI ScaI SfiI SfuI SgrAI SpeI SspI SwaI Tth111I XbaI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AccI 3610 GTCTAC GT^[AC][GT]AC AccIII 280 TCCGGA T^CCGGA AhdI 2335 GACTGTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 929 CCTCAGG CC^T[ACGT]AGG Asp700I 3423 GAACTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 2335 GACTGTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AviII 1703 TGCGCA TGC^GCA BbeI 3148 GGCGCC GGCGC^C BglII 1631 AGATCT A^GATCT BsaI 2572 GGTCTC GGTCTC(1/5) BseAI 280 TCCGGA T^CCGGA BsmBI 3493 CGTCTC CGTCTC(1/5) BsmI 2285 GAATGC GAATGC(1/-1) BspEI 280 TCCGGA T^CCGGA BspLU11I 3614 ACATGT A^CATGT BssSI 535 CTCGTG CACGAG(-5/-1) BstEII 1056 GGTGACC G^GT[ACGT]ACC BstPI 1056 GGTGACC G^GT[ACGT]ACC Bsu36I 929 CCTCAGG CC^T[ACGT]AGG DrdI 2794 GACTGCTACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 2335 GACTGTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 929 CCTCAGG CC^T[ACGT]AGG EcoNI 141 CCTGCCCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 1056 GGTGACC G^GT[ACGT]ACC FspI 1703 TGCGCA TGC^GCA HindIII 1673 AAGCTT A^AGCTT KasI 3148 GGCGCC G^GCGCC MroI 280 TCCGGA T^CCGGA MseI 2036 TTAA T^TAA NaeI 3719 GCCGGC GCC^GGC NarI 3148 GGCGCC GG^CGCC NgoMIV 3719 GCCGGC G^CCGGC NheI 1203 GCTAGC G^CTAGC NotI 3711 GCGGCCGC GC^GGCCGC PaeR7I 2467 CTCGAG C^TCGAG PflMI 1431 CCAGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG SacI 2092 GAGCTC GAGCT^C SapI 435 GCTCTTC GCTCTTC(1/4) SbfI 1770 CCTGCAGG CCTGCA^GG SfoI 3148 GGCGCC GGC^GCC SnaBI 1969 TACGTA TAC^GTA SphI 561 GCATGC GCATG^C Sse8387I 1770 CCTGCAGG CCTGCA^GG StuI 3665 AGGCCT AGG^CCT TfiI 1303 GATTC G^A[AT]TC Tru9I 2036 TTAA T^TAA Van91I 1431 CCAGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 2467 CTCGAG C^TCGAG XmnI 3423 GAACTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 35 enzymes) # ################################### AcsI 276 GAATTC [GA]^AATT[CT] 897 GAATTC AcyI 2596 GACGCC G[GA]^CG[CT]C 3148 GGCGCC Alw44I 520 GTGCAC G^TGCAC 805 GTGCAC AlwI 3174 GATCC GGATC(4/5) 684 GGATC ApaLI 520 GTGCAC G^TGCAC 805 GTGCAC ApoI 276 GAATTC [GA]^AATT[CT] 897 GAATTC BalI 2775 TGGCCA TGG^CCA 3600 TGGCCA BbrPI 808 CACGTG CAC^GTG 2146 CACGTG BlpI 870 GCTGAGC GC^T[ACGT]AGC 3654 GCTGAGC Bpu1102I 870 GCTGAGC GC^T[ACGT]AGC 3654 GCTGAGC BsaHI 2596 GACGCC G[GA]^CG[CT]C 3148 GGCGCC BsaWI 280 TCCGGA [AT]^CCGG[AT] 3500 TCCGGT BsiEI 2364 CGGCCG CG[GA][CT]^CG 3712 CGGCCG BsrBI 2047 GAGCGG CCGCTC(-3/-3) 3709 GAGCGG BsrFI 2435 ACCGGC [GA]^CCGG[CT] 3719 GCCGGC BssHII 243 GCGCGC G^CGCGC 2388 GCGCGC CelII 870 GCTGAGC GC^T[ACGT]AGC 3654 GCTGAGC Cfr10I 2435 ACCGGC [GA]^CCGG[CT] 3719 GCCGGC EagI 2364 CGGCCG C^GGCCG 3712 CGGCCG EclXI 2364 CGGCCG C^GGCCG 3712 CGGCCG Eco52I 2364 CGGCCG C^GGCCG 3712 CGGCCG EcoRI 276 GAATTC G^AATTC 897 GAATTC Hin1I 2596 GACGCC G[GA]^CG[CT]C 3148 GGCGCC HincII 1696 GTCAAC GT[CT]^[GA]AC 2523 GTTGAC HindII 1696 GTCAAC GT[CT]^[GA]AC 2523 GTTGAC KspI 358 CCGCGG CCGC^GG 2647 CCGCGG MluNI 2775 TGGCCA TGG^CCA 3600 TGGCCA MscI 2775 TGGCCA TGG^CCA 3600 TGGCCA PmaCI 808 CACGTG CAC^GTG 2146 CACGTG PmlI 808 CACGTG CAC^GTG 2146 CACGTG SacII 358 CCGCGG CCGC^GG 2647 CCGCGG SexAI 1052 ACCTGGT A^CC[AT]GGT 1124 ACCTGGT SmaI 1397 CCCGGG CCC^GGG 2568 CCCGGG XmaCI 1397 CCCGGG C^CCGGG 2568 CCCGGG XmaI 1397 CCCGGG C^CCGGG 2568 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 110 enzymes) # ######################################### AccII AciI AfaI AflIII AluI AlwNI ApaI AspHI AvaI AvaII BanI BanII BbsI BbvCI BbvI BcnI BfaI BglI BmyI BpmI BpuAI BsaAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I Bsp1407I BspMI BsrDI BsrGI BsrI BssKI BstAPI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI StyI TaqI TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII