(Circular) (Six-base) MAPSORT of: pFN21AE1457.seq Check: 4187 from: 1 to: 8512 pFN21AE1457 8512 bp With 182 enzymes: SgfI * December 8, 2014 13:44 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2538 2625 3006 3325 3769 4480 2538 Size: 87 381 319 444 711 6570 Fragments arranged by size: 6570 711 444 381 319 87 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6322 278 Size: 53 83 186 936 4786 2468 Fragments arranged by size: 4786 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5616 5855 1937 Size: 3679 239 4594 Fragments arranged by size: 4594 3679 239 Acc65I G'GTAC_C Cuts at: 5833 5833 Size: 8512 AclI AA'CG_TT Cuts at: 1563 6639 7012 1563 Size: 5076 373 3063 Fragments arranged by size: 5076 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2138 2297 2906 3362 4179 4993 5687 6575 Size: 506 159 609 456 817 814 694 888 Cuts at: 6575 7959 8227 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1384 888 817 814 694 609 506 456 268 159 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8315 AflIII A'CryG_T Cuts at: 1203 4353 5495 5619 7411 8388 1203 Size: 3150 1142 124 1792 977 1327 Fragments arranged by size: 3150 1792 1327 1142 977 124 AgeI A'CCGG_T Cuts at: 7343 7343 Size: 8512 AhdI GACnn_n'nnGTC Cuts at: 4345 7241 4345 Size: 2896 5616 AleI CACnn'nnGTG Cuts at: 1166 2018 1166 Size: 852 7660 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 8512 AlwNI CAG_nnn'CTG Cuts at: 2176 2611 3340 3442 4309 4423 4824 5336 5540 Size: 435 729 102 867 114 401 512 204 Cuts at: 5540 6255 7827 2176 Size: 715 1572 2861 Fragments arranged by size: 2861 1572 867 729 715 512 435 401 204 114 102 ApaI G_GGCC'C Cuts at: 3264 3409 3991 3264 Size: 145 582 7785 Fragments arranged by size: 7785 582 145 ApaLI G'TGCA_C Cuts at: 2525 2810 6569 7725 2525 Size: 285 3759 1156 3312 Fragments arranged by size: 3759 3312 1156 285 ApoI r'AATT_y Cuts at: 1493 2281 2902 5821 6047 6199 8308 1493 Size: 788 621 2919 226 152 2109 1697 Fragments arranged by size: 2919 2109 1697 788 621 226 152 AseI AT'TA_AT Cuts at: 160 1032 7066 160 Size: 872 6034 1606 Fragments arranged by size: 6034 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8512 AvaI C'yCGr_G Cuts at: 1870 1943 2150 2262 2720 3402 4472 4573 5837 Size: 73 207 112 458 682 1070 101 1264 Cuts at: 5837 1870 Size: 4545 Fragments arranged by size: 4545 1264 1070 682 458 207 112 101 73 BamHI G'GATC_C Cuts at: 5842 6209 5842 Size: 367 8145 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2716 3018 3215 4478 4828 Size: 325 217 627 929 302 197 1263 350 Cuts at: 4828 5153 5833 7288 618 Size: 325 680 1455 1842 Fragments arranged by size: 1842 1455 1263 929 680 627 350 325 325 302 217 197 BanII G_rGCy'C Cuts at: 1391 3264 3409 3991 4101 4134 4574 5831 1391 Size: 1873 145 582 110 33 440 1257 4072 Fragments arranged by size: 4072 1873 1257 582 440 145 110 33 BbeI G_GCGC'C Cuts at: 5157 5157 Size: 8512 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3849 4167 4716 4801 4869 5364 928 Size: 971 1950 318 549 85 68 495 4076 Fragments arranged by size: 4076 1950 971 549 495 318 85 68 BbvCI CC'TCA_GC Cuts at: 2048 2405 2477 2762 2860 3029 4911 2048 Size: 357 72 285 98 169 1882 5649 Fragments arranged by size: 5649 1882 357 285 169 98 72 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6703 6737 1903 Size: 34 4766 34 3678 Fragments arranged by size: 4766 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6404 7614 6404 Size: 1210 7302 BclI T'GATC_A Cuts at: 1548 1548 Size: 8512 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2258 4476 Size: 107 122 71 631 605 337 249 2218 Cuts at: 4476 5351 5725 7123 136 Size: 875 374 1398 1525 Fragments arranged by size: 2218 1525 1398 875 631 605 374 337 249 122 107 71 BglII A'GATC_T Cuts at: 3636 8507 3636 Size: 4871 3641 BlpI GC'TnA_GC Cuts at: 2876 5660 5929 2876 Size: 2784 269 5459 Fragments arranged by size: 5459 2784 269 Bme1580I G_kGCm'C Cuts at: 1790 2363 2529 2721 2814 3023 3264 3409 3912 Size: 573 166 192 93 209 241 145 503 Cuts at: 3912 3991 4481 5455 6573 7729 1790 Size: 79 490 974 1118 1156 2573 Fragments arranged by size: 2573 1156 1118 974 573 503 490 241 209 192 166 145 93 79 BmgBI CAC'GTC Cuts at: 1318 2686 3487 4354 5498 1318 Size: 1368 801 867 1144 4332 Fragments arranged by size: 4332 1368 1144 867 801 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3640 4130 4834 Size: 416 53 83 399 24 2223 490 704 Cuts at: 4834 6243 7201 442 Size: 1409 958 1753 Fragments arranged by size: 2223 1753 1409 958 704 490 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3212 1056 Size: 2156 6356 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5566 5566 Size: 8512 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2393 2513 2681 3011 3464 4238 4760 5072 Size: 1154 120 168 330 453 774 522 312 Cuts at: 5072 5218 5806 7172 1239 Size: 146 588 1366 2579 Fragments arranged by size: 2579 1366 1154 774 588 522 453 330 312 168 146 120 Bpu10I CC'TnA_GC Cuts at: 2048 2405 2477 2762 2860 3029 4911 2048 Size: 357 72 285 98 169 1882 5649 Fragments arranged by size: 5649 1882 357 285 169 98 72 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2356 3581 3899 4391 5252 6637 7502 7800 2356 Size: 1225 318 492 861 1385 865 298 3068 Fragments arranged by size: 3068 1385 1225 865 861 492 318 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4583 7175 882 Size: 617 3084 2592 2219 Fragments arranged by size: 3084 2592 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2815 3976 4153 6303 493 Size: 711 1611 1161 177 2150 2702 Fragments arranged by size: 2702 2150 1611 1161 711 177 BsaBI GATnn'nnATC Cuts at: 8506 8506 Size: 8512 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4602 5154 6319 6701 Size: 53 83 186 936 3069 552 1165 382 Cuts at: 6701 7336 275 Size: 635 1451 Fragments arranged by size: 3069 1451 1165 936 635 552 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2285 5505 6945 7343 7617 7764 8175 2285 Size: 3220 1440 398 274 147 411 2622 Fragments arranged by size: 3220 2622 1440 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3605 3635 3782 3812 4229 Size: 30 165 30 2202 30 147 30 417 Cuts at: 4229 4259 5216 5246 1178 Size: 30 957 30 4444 Fragments arranged by size: 4444 2202 957 417 165 147 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2168 3401 3761 3782 4712 4787 4859 4862 Size: 982 1233 360 21 930 75 72 3 Cuts at: 4862 4886 4934 4949 5231 5432 1186 Size: 24 48 15 282 201 4266 Fragments arranged by size: 4266 1233 982 930 360 282 201 75 72 48 24 21 15 3 BseYI C'CCAG_C Cuts at: 2587 2628 2741 3410 4411 5165 7715 8211 2587 Size: 41 113 669 1001 754 2550 496 2888 Fragments arranged by size: 2888 2550 1001 754 669 496 113 41 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2597 4106 4421 5188 1163 Size: 573 861 1509 315 767 4487 Fragments arranged by size: 4487 1509 861 767 573 315 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4372 5720 5962 6723 7751 1540 Size: 275 186 2371 1348 242 761 1028 2301 Fragments arranged by size: 2371 2301 1348 1028 761 275 242 186 BsiHKAI G_wGCw'C Cuts at: 2529 2547 2627 2814 3597 4101 5513 6573 6658 Size: 18 80 187 783 504 1412 1060 85 Cuts at: 6658 7729 2529 Size: 1071 3312 Fragments arranged by size: 3312 1412 1071 1060 783 504 187 85 80 18 BsmI GAATG_Cn' Cuts at: 4296 6023 6116 4296 Size: 1727 93 6692 Fragments arranged by size: 6692 1727 93 BsmBI CGTCTCn'nnnn_ Cuts at: 5504 5504 Size: 8512 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2363 2529 2547 2627 2721 2814 3023 Size: 399 573 166 18 80 94 93 209 Cuts at: 3023 3264 3409 3597 3912 3991 4101 4134 4481 Size: 241 145 188 315 79 110 33 347 Cuts at: 4481 4574 5455 5513 5831 6573 6658 7729 1391 Size: 93 881 58 318 742 85 1071 2174 Fragments arranged by size: 2174 1071 881 742 573 399 347 318 315 241 209 188 166 145 110 94 93 93 85 80 79 58 33 18 BspEI T'CCGG_A Cuts at: 2285 8175 2285 Size: 5890 2622 BspHI T'CATG_A Cuts at: 6401 8131 6401 Size: 1730 6782 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2154 2538 2625 2655 3006 3325 3769 4480 Size: 1310 384 87 30 351 319 444 711 Cuts at: 4480 4809 5389 5867 844 Size: 329 580 478 3489 Fragments arranged by size: 3489 1310 711 580 478 444 384 351 329 319 87 30 BsrBI CCG'CTC Cuts at: 1394 1823 4054 5716 6399 1394 Size: 429 2231 1662 683 3507 Fragments arranged by size: 3507 2231 1662 683 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2707 4555 5755 7007 7181 65 Size: 1183 1459 1848 1200 1252 174 1396 Fragments arranged by size: 1848 1459 1396 1252 1200 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4440 5724 7156 7343 1700 Size: 110 2630 1284 1432 187 2869 Fragments arranged by size: 2869 2630 1432 1284 187 110 BsrGI T'GTAC_A Cuts at: 96 2730 4188 4329 96 Size: 2634 1458 141 4279 Fragments arranged by size: 4279 2634 1458 141 BssHII G'CGCG_C Cuts at: 1925 2248 4393 1925 Size: 323 2145 6044 Fragments arranged by size: 6044 2145 323 BssSI C'ACGA_G Cuts at: 2540 6572 7584 2540 Size: 4032 1012 3468 Fragments arranged by size: 4032 3468 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2611 3158 4309 5211 794 Size: 1817 547 1151 902 4095 Fragments arranged by size: 4095 1817 1151 902 547 BstBI TT'CG_AA Cuts at: 7316 7316 Size: 8512 BstEII G'GTnAC_C Cuts at: 1184 3061 1184 Size: 1877 6635 BstXI CCAn_nnnn'nTGG Cuts at: 2659 2914 4110 4672 2659 Size: 255 1196 562 6499 Fragments arranged by size: 6499 1196 562 255 BstYI r'GATC_y Cuts at: 1972 2689 3636 5178 5842 6209 6594 6611 8063 Size: 717 947 1542 664 367 385 17 1452 Cuts at: 8063 8149 8161 8507 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1542 1452 947 717 664 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2935 2935 Size: 8512 BtgI C'CryG_G Cuts at: 513 1065 2004 2363 3231 3559 4248 4455 4652 Size: 552 939 359 868 328 689 207 197 Cuts at: 4652 513 Size: 4373 Fragments arranged by size: 4373 939 868 689 552 359 328 207 197 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2226 2606 3771 487 Size: 683 56 1000 380 1165 5228 Fragments arranged by size: 5228 1165 1000 683 380 56 BtsI GCAGTG_nn' Cuts at: 2852 4076 4635 5541 6028 6822 6842 2852 Size: 1224 559 906 487 794 20 4522 Fragments arranged by size: 4522 1224 906 794 559 487 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8512 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8477 DraI TTT'AAA Cuts at: 5816 5816 Size: 8512 DraIII CAC_nnn'GTG Cuts at: 3061 4124 5211 3061 Size: 1063 1087 6362 Fragments arranged by size: 6362 1087 1063 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4805 7519 809 Size: 507 3489 2714 1802 Fragments arranged by size: 3489 2714 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2160 4169 4369 Size: 54 1425 50 275 18 330 2009 200 Cuts at: 4369 4442 4674 4780 5605 5717 5808 5959 6848 Size: 73 232 106 825 112 91 151 889 Cuts at: 6848 8477 8 Size: 1629 43 Fragments arranged by size: 2009 1629 1425 889 825 330 275 232 200 151 112 106 91 73 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4369 5717 5959 1537 Size: 275 2557 1348 242 4090 Fragments arranged by size: 4090 2557 1348 275 242 EarI CTCTTCn'nnn_ Cuts at: 1353 2447 2958 4764 5042 5507 6442 1353 Size: 1094 511 1806 278 465 935 3423 Fragments arranged by size: 3423 1806 1094 935 511 465 278 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4697 7099 7473 7619 387 Size: 1064 138 3108 2402 374 146 1280 Fragments arranged by size: 3108 2402 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4099 4099 Size: 8512 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2138 2297 2393 2513 2681 2906 3011 Size: 393 506 159 96 120 168 225 105 Cuts at: 3011 3362 3464 4179 4238 4760 4993 5072 5218 Size: 351 102 715 59 522 233 79 146 Cuts at: 5218 5687 5806 6575 7172 7959 8227 1239 Size: 469 119 769 597 787 268 1524 Fragments arranged by size: 1524 787 769 715 597 522 506 469 393 351 268 233 225 168 159 146 120 119 105 102 96 79 59 EcoNI CCTnn'n_nnAGG Cuts at: 2150 2150 Size: 8512 EcoO109I rG'GnC_Cy Cuts at: 3103 3268 3406 3439 3921 3987 5631 5803 3103 Size: 165 138 33 482 66 1644 172 5812 Fragments arranged by size: 5812 1644 482 172 165 138 66 33 EcoRI G'AATT_C Cuts at: 2281 2902 5821 6199 2281 Size: 621 2919 378 4594 Fragments arranged by size: 4594 2919 621 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2935 5293 5545 2935 Size: 2358 252 5902 Fragments arranged by size: 5902 2358 252 FspI TGC'GCA Cuts at: 3710 7018 3710 Size: 3308 5204 HaeII r_GCGC'y Cuts at: 1678 5157 5199 5793 7659 1678 Size: 3479 42 594 1866 2531 Fragments arranged by size: 3479 2531 1866 594 42 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2306 2338 3495 3527 3605 Size: 32 135 32 734 32 1157 32 78 Cuts at: 3605 3637 4331 4363 4561 4593 7153 7185 7227 Size: 32 694 32 198 32 2560 32 42 Cuts at: 7227 7259 1373 Size: 32 2626 Fragments arranged by size: 2626 2560 1157 734 694 198 135 78 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3703 4530 5856 6102 669 Size: 988 281 1765 827 1326 246 3079 Fragments arranged by size: 3079 1765 1326 988 827 281 246 HindIII A'AGCT_T Cuts at: 748 3678 748 Size: 2930 5582 HpaI GTT'AAC Cuts at: 6102 6102 Size: 8512 KasI G'GCGC_C Cuts at: 5153 5153 Size: 8512 KpnI G_GTAC'C Cuts at: 5837 5837 Size: 8512 MfeI C'AATT_G Cuts at: 6111 6111 Size: 8512 MluI A'CGCG_T Cuts at: 8388 8388 Size: 8512 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2931 3879 4374 5538 5577 7626 7810 1305 Size: 1626 948 495 1164 39 2049 184 2007 Fragments arranged by size: 2049 2007 1626 1164 948 495 184 39 MscI TGG'CCA Cuts at: 10 64 1489 1832 4782 5607 10 Size: 54 1425 343 2950 825 2915 Fragments arranged by size: 2950 2915 1425 825 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2018 2393 3863 5624 6470 6829 6988 Size: 648 852 375 1470 1761 846 359 159 Cuts at: 6988 518 Size: 2042 Fragments arranged by size: 2042 1761 1470 852 846 648 375 359 159 MspA1I CmG'CkG Cuts at: 1606 2052 2350 2365 3164 3202 3242 3248 3414 Size: 446 298 15 799 38 40 6 166 Cuts at: 3414 3496 3618 4654 4690 4910 5772 5928 6605 Size: 82 122 1036 36 220 862 156 677 Cuts at: 6605 7753 7998 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 1036 862 799 677 446 298 245 220 166 156 122 82 40 38 36 15 6 NaeI GCC'GGC Cuts at: 5726 5726 Size: 8512 NarI GG'CG_CC Cuts at: 5154 5154 Size: 8512 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 7021 Fragments arranged by size: 7021 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 8512 NgoMIV G'CCGG_C Cuts at: 5724 5724 Size: 8512 NheI G'CTAG_C Cuts at: 1052 3208 1052 Size: 2156 6356 NotI GC'GGCC_GC Cuts at: 5717 5959 5717 Size: 242 8270 NspI r_CATG'y Cuts at: 1132 1229 2392 2570 3352 5623 5698 5870 7415 Size: 97 1163 178 782 2271 75 172 1545 Cuts at: 7415 1132 Size: 2229 Fragments arranged by size: 2271 2229 1545 1163 782 178 172 97 75 PasI CC'CwG_GG Cuts at: 1348 5042 1348 Size: 3694 4818 PciI A'CATG_T Cuts at: 5619 7411 5619 Size: 1792 6720 PflMI CCAn_nnn'nTGG Cuts at: 1108 3442 1108 Size: 2334 6178 PfoI T'CCnGG_A Cuts at: 2328 3105 3741 4583 2328 Size: 777 636 842 6257 Fragments arranged by size: 6257 842 777 636 PmeI GTTT'AAAC Cuts at: 5816 5816 Size: 8512 PmlI CAC'GTG Cuts at: 2815 4153 2815 Size: 1338 7174 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6581 6613 8094 8126 6581 Size: 32 1481 32 6967 Fragments arranged by size: 6967 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3103 3268 3921 5631 3103 Size: 165 653 1710 5984 Fragments arranged by size: 5984 1710 653 165 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8512 PsiI TTA'TAA Cuts at: 6082 6082 Size: 8512 PspOMI G'GGCC_C Cuts at: 3260 3405 3987 3260 Size: 145 582 7785 Fragments arranged by size: 7785 582 145 PspXI vC'TCGA_Gb Cuts at: 4472 4472 Size: 8512 PstI C_TGCA'G Cuts at: 830 2172 2535 2607 3017 3195 3241 3247 3253 Size: 1342 363 72 410 178 46 6 6 Cuts at: 3253 3780 4305 4569 4820 5864 830 Size: 527 525 264 251 1044 3478 Fragments arranged by size: 3478 1342 1044 527 525 410 363 264 251 178 72 46 6 6 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8512 PvuII CAG'CTG Cuts at: 2350 3202 3242 3248 3496 4690 2350 Size: 852 40 6 248 1194 6172 Fragments arranged by size: 6172 1194 852 248 40 6 SacI G_AGCT'C Cuts at: 4101 4101 Size: 8512 SacII CC_GC'GG Cuts at: 2366 4655 2366 Size: 2289 6223 SalI G'TCGA_C Cuts at: 1936 5854 1936 Size: 3918 4594 SanDI GG'GwC_CC Cuts at: 5631 5631 Size: 8512 SapI GCTCTTCn'nnn_ Cuts at: 2447 2447 Size: 8512 SbfI CC_TGCA'GG Cuts at: 3780 5864 3780 Size: 2084 6428 ScaI AGT'ACT Cuts at: 6760 8472 6760 Size: 1712 6800 SexAI A'CCwGG_T Cuts at: 3057 3129 3057 Size: 72 8440 SfcI C'TryA_G Cuts at: 826 1045 2168 2531 2603 3013 3191 3237 3243 Size: 219 1123 363 72 410 178 46 6 Cuts at: 3243 3249 3776 4301 4565 4816 5389 5860 6995 Size: 6 527 525 264 251 573 471 1135 Cuts at: 6995 7676 7867 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 1123 681 573 527 525 471 410 363 264 251 219 191 178 72 46 6 6 SfoI GGC'GCC Cuts at: 5155 5155 Size: 8512 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8512 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8512 SmaI CCC'GGG Cuts at: 1872 3404 4575 5839 1872 Size: 1532 1171 1264 4545 Fragments arranged by size: 4545 1532 1264 1171 SmlI C'TyrA_G Cuts at: 820 1017 1943 2335 3596 3914 4406 4472 5267 Size: 197 926 392 1261 318 492 66 795 Cuts at: 5267 6616 7517 7779 820 Size: 1349 901 262 1553 Fragments arranged by size: 1553 1349 1261 926 901 795 492 392 318 262 197 66 SnaBI TAC'GTA Cuts at: 493 3976 6303 493 Size: 3483 2327 2702 Fragments arranged by size: 3483 2702 2327 SpeI A'CTAG_T Cuts at: 152 152 Size: 8512 SphI G_CATG'C Cuts at: 1132 2570 5870 1132 Size: 1438 3300 3774 Fragments arranged by size: 3774 3300 1438 SrfI GCCC'GGGC Cuts at: 3404 3404 Size: 8512 SspI AAT'ATT Cuts at: 5 52 6436 5 Size: 47 6384 2081 Fragments arranged by size: 6384 2081 47 StuI AGG'CCT Cuts at: 1517 5672 1517 Size: 4155 4357 StyI C'CwwG_G Cuts at: 513 1065 2004 3264 3935 4014 4269 5703 5951 Size: 552 939 1260 671 79 255 1434 248 Cuts at: 5951 513 Size: 3074 Fragments arranged by size: 3074 1434 1260 939 671 552 255 248 79 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2642 2651 2797 3710 3972 4112 4816 Size: 1255 1062 9 146 913 262 140 704 Cuts at: 4816 5305 5428 5614 6539 6556 6709 6894 7339 Size: 489 123 186 925 17 153 185 445 Cuts at: 7339 325 Size: 1498 Fragments arranged by size: 1498 1255 1062 925 913 704 489 445 262 186 185 153 146 140 123 17 9 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2730 4188 4329 Size: 274 80 33 51 1443 753 1458 141 Cuts at: 4329 4956 5481 6758 8470 96 Size: 627 525 1277 1712 138 Fragments arranged by size: 1712 1458 1443 1277 753 627 525 274 141 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3856 3888 4931 4963 5487 5519 5684 5716 8472 Size: 32 1043 32 524 32 165 32 2756 Cuts at: 8472 8504 3856 Size: 32 3864 Fragments arranged by size: 3864 2756 1043 524 165 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8512 XbaI T'CTAG_A Cuts at: 5848 5848 Size: 8512 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2389 2986 3271 2389 Size: 597 285 7630 Fragments arranged by size: 7630 597 285 XhoI C'TCGA_G Cuts at: 1943 4472 1943 Size: 2529 5983 XmaI C'CCGG_G Cuts at: 1870 3402 4573 5837 1870 Size: 1532 1171 1264 4545 Fragments arranged by size: 4545 1532 1264 1171 XmnI GAAnn'nnTTC Cuts at: 5432 6641 5432 Size: 1209 7303 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6320 276 Size: 53 83 186 936 4786 2468 Fragments arranged by size: 4786 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I EcoRV FseI FspAI NruI NsiI PacI PsrI RsrII SfiI SwaI