(Circular) (Six-base) MAPSORT of: pFN21AB7054.seq Check: 6116 from: 1 to: 6427 pFN21AB7054 6427 bp With 182 enzymes: SgfI * July 6, 2012 17:34 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3431 3431 Size: 6427 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4237 278 Size: 53 83 186 936 2701 2468 Fragments arranged by size: 2701 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3770 1937 Size: 1833 4594 Acc65I G'GTAC_C Cuts at: 3748 3748 Size: 6427 AclI AA'CG_TT Cuts at: 1563 4554 4927 1563 Size: 2991 373 3063 Fragments arranged by size: 3063 2991 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3066 4490 5874 6142 1632 Size: 1434 1424 1384 268 1917 Fragments arranged by size: 1917 1434 1424 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6230 AflIII A'CryG_T Cuts at: 1203 3431 3488 5326 6303 1203 Size: 2228 57 1838 977 1327 Fragments arranged by size: 2228 1838 1327 977 57 AgeI A'CCGG_T Cuts at: 5258 5258 Size: 6427 AhdI GACnn_n'nnGTC Cuts at: 5156 5156 Size: 6427 AleI CACnn'nnGTG Cuts at: 1166 3489 1166 Size: 2323 4104 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6427 AlwNI CAG_nnn'CTG Cuts at: 2182 2185 2798 3005 4170 5742 2182 Size: 3 613 207 1165 1572 2867 Fragments arranged by size: 2867 1572 1165 613 207 3 ApaI G_GGCC'C Cuts at: 2134 2524 2134 Size: 390 6037 ApaLI G'TGCA_C Cuts at: 3482 4484 5640 3482 Size: 1002 1156 4269 Fragments arranged by size: 4269 1156 1002 ApoI r'AATT_y Cuts at: 1493 3736 3962 4114 6223 1493 Size: 2243 226 152 2109 1697 Fragments arranged by size: 2243 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4981 160 Size: 872 3949 1606 Fragments arranged by size: 3949 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6427 AvaI C'yCGr_G Cuts at: 1870 1943 2517 3752 1870 Size: 73 574 1235 4545 Fragments arranged by size: 4545 1235 574 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2424 2457 2424 Size: 33 6394 BamHI G'GATC_C Cuts at: 2941 3757 4124 2941 Size: 816 367 5244 Fragments arranged by size: 5244 816 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2609 2779 3748 5203 618 Size: 325 217 627 822 170 969 1455 1842 Fragments arranged by size: 1842 1455 969 822 627 325 217 170 BanII G_rGCy'C Cuts at: 1391 2134 2524 3746 1391 Size: 743 390 1222 4072 Fragments arranged by size: 4072 1222 743 390 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3204 3357 3446 928 Size: 971 1305 153 89 3909 Fragments arranged by size: 3909 1305 971 153 89 BbvCI CC'TCA_GC Cuts at: 2980 2980 Size: 6427 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3284 3318 4618 4652 1903 Size: 34 50 34 1263 34 1300 34 3678 Fragments arranged by size: 3678 1300 1263 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2425 4319 5529 2425 Size: 1894 1210 3323 Fragments arranged by size: 3323 1894 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6427 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2270 2652 5038 Size: 107 122 71 631 605 598 382 2386 Cuts at: 5038 136 Size: 1525 Fragments arranged by size: 2386 1525 631 605 598 382 122 107 71 BglII A'GATC_T Cuts at: 2069 6422 2069 Size: 4353 2074 BlpI GC'TnA_GC Cuts at: 3051 3844 3051 Size: 793 5634 Bme1580I G_kGCm'C Cuts at: 1790 2134 2524 2782 3172 3486 4488 5644 1790 Size: 344 390 258 390 314 1002 1156 2573 Fragments arranged by size: 2573 1156 1002 390 390 344 314 258 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6427 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2248 2659 4158 Size: 416 53 83 399 24 831 411 1499 Cuts at: 4158 5116 442 Size: 958 1753 Fragments arranged by size: 1753 1499 958 831 416 411 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6427 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2688 2967 3000 3018 3171 3239 3369 5087 Size: 1449 279 33 18 153 68 130 1718 Cuts at: 5087 1239 Size: 2579 Fragments arranged by size: 2579 1718 1449 279 153 130 68 33 18 Bpu10I CC'TnA_GC Cuts at: 2009 2980 2009 Size: 971 5456 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4552 5417 5715 4552 Size: 865 298 5264 Fragments arranged by size: 5264 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2828 5090 882 Size: 617 1329 2262 2219 Fragments arranged by size: 2262 2219 1329 617 BsaAI yAC'GTr Cuts at: 493 1204 3491 4218 493 Size: 711 2287 727 2702 Fragments arranged by size: 2702 2287 727 711 BsaBI GATnn'nnATC Cuts at: 6421 6421 Size: 6427 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4234 4616 5251 275 Size: 53 83 186 936 2701 382 635 1451 Fragments arranged by size: 2701 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4860 5258 5532 5679 6090 4860 Size: 398 274 147 411 5197 Fragments arranged by size: 5197 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2192 2222 2576 2606 3144 Size: 30 165 30 789 30 354 30 538 Cuts at: 3144 3174 1178 Size: 30 4431 Fragments arranged by size: 4431 789 538 354 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2567 3168 3236 1186 Size: 1381 601 68 4377 Fragments arranged by size: 4377 1381 601 68 BseYI C'CCAG_C Cuts at: 2374 2974 2998 3040 3180 5630 6126 2374 Size: 600 24 42 140 2450 496 2675 Fragments arranged by size: 2675 2450 600 496 140 42 24 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2215 3309 3315 3653 1163 Size: 573 479 1094 6 338 3937 Fragments arranged by size: 3937 1094 573 479 338 6 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3877 4638 5666 1540 Size: 275 186 1876 761 1028 2301 Fragments arranged by size: 2301 1876 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3486 4488 4573 5644 3486 Size: 1002 85 1071 4269 Fragments arranged by size: 4269 1071 1002 85 BsmI GAATG_Cn' Cuts at: 3938 4031 3938 Size: 93 6334 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2134 2524 2782 3172 3486 3746 4488 Size: 399 344 390 258 390 314 260 742 Cuts at: 4488 4573 5644 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 390 390 344 314 260 258 85 BspEI T'CCGG_A Cuts at: 6090 6090 Size: 6427 BspHI T'CATG_A Cuts at: 4316 6046 4316 Size: 1730 4697 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3431 3782 844 Size: 2587 351 3489 Fragments arranged by size: 3489 2587 351 BsrBI CCG'CTC Cuts at: 1394 1823 2320 4314 1394 Size: 429 497 1994 3507 Fragments arranged by size: 3507 1994 497 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4922 5096 65 Size: 1183 3674 174 1396 Fragments arranged by size: 3674 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5071 5258 1700 Size: 110 3261 187 2869 Fragments arranged by size: 3261 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6427 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6427 BssSI C'ACGA_G Cuts at: 4487 5499 4487 Size: 1012 5415 BstAPI GCAn_nnn'nTGC Cuts at: 794 2185 794 Size: 1391 5036 BstBI TT'CG_AA Cuts at: 5231 5231 Size: 6427 BstEII G'GTnAC_C Cuts at: 1184 3313 1184 Size: 2129 4298 BstYI r'GATC_y Cuts at: 1972 2069 2338 2941 3604 3757 4124 4509 4526 Size: 97 269 603 663 153 367 385 17 Cuts at: 4526 5978 6064 6076 6422 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 663 603 385 367 346 269 153 97 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3123 3123 Size: 6427 BtgI C'CryG_G Cuts at: 513 1065 2605 3477 513 Size: 552 1540 872 3463 Fragments arranged by size: 3463 1540 872 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2025 487 Size: 683 56 799 4889 Fragments arranged by size: 4889 799 683 56 BtsI GCAGTG_nn' Cuts at: 2888 2954 3165 3682 3943 4737 4757 2888 Size: 66 211 517 261 794 20 4558 Fragments arranged by size: 4558 794 517 261 211 66 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6427 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3415 3450 529 Size: 35 2851 35 3506 Fragments arranged by size: 3506 2851 35 35 DraI TTT'AAA Cuts at: 3731 3731 Size: 6427 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3106 5434 809 Size: 507 1790 2328 1802 Fragments arranged by size: 2328 1802 1790 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2410 3585 3874 Size: 54 1425 50 275 18 580 1175 289 Cuts at: 3874 4763 6392 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1175 889 580 289 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3874 1537 Size: 275 2062 4090 Fragments arranged by size: 4090 2062 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2863 3090 4357 1353 Size: 1510 227 1267 3423 Fragments arranged by size: 3423 1510 1267 227 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5014 5388 5534 387 Size: 1064 138 3425 374 146 1280 Fragments arranged by size: 3425 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2688 2967 3000 3018 3066 3171 3239 Size: 393 1056 279 33 18 48 105 68 Cuts at: 3239 3369 4490 5087 5874 6142 1239 Size: 130 1121 597 787 268 1524 Fragments arranged by size: 1524 1121 1056 787 597 393 279 268 130 105 68 48 33 18 EcoNI CCTnn'n_nnAGG Cuts at: 2528 2528 Size: 6427 EcoO109I rG'GnC_Cy Cuts at: 2119 2131 2521 2909 3128 3544 3580 3617 2119 Size: 12 390 388 219 416 36 37 4929 Fragments arranged by size: 4929 416 390 388 219 37 36 12 EcoRI G'AATT_C Cuts at: 3736 4114 3736 Size: 378 6049 FspI TGC'GCA Cuts at: 4933 4933 Size: 6427 HaeII r_GCGC'y Cuts at: 1678 5574 1678 Size: 3896 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2192 2224 2576 2608 3307 Size: 32 135 32 620 32 352 32 699 Cuts at: 3307 3339 5068 5100 5142 5174 1373 Size: 32 1729 32 42 32 2626 Fragments arranged by size: 2626 1729 699 620 352 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2761 3771 4017 669 Size: 988 281 823 1010 246 3079 Fragments arranged by size: 3079 1010 988 823 281 246 HindIII A'AGCT_T Cuts at: 748 3391 748 Size: 2643 3784 HpaI GTT'AAC Cuts at: 4017 4017 Size: 6427 KpnI G_GTAC'C Cuts at: 3752 3752 Size: 6427 MfeI C'AATT_G Cuts at: 4026 4026 Size: 6427 MluI A'CGCG_T Cuts at: 6303 6303 Size: 6427 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2071 5541 5725 1305 Size: 766 3470 184 2007 Fragments arranged by size: 3470 2007 766 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2412 3587 10 Size: 54 1425 343 580 1175 2850 Fragments arranged by size: 2850 1425 1175 580 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2237 2478 3489 4385 4744 4903 518 Size: 648 1071 241 1011 896 359 159 2042 Fragments arranged by size: 2042 1071 1011 896 648 359 241 159 MspA1I CmG'CkG Cuts at: 1606 2182 2206 2416 2459 3044 3843 4520 5668 Size: 576 24 210 43 585 799 677 1148 Cuts at: 5668 5913 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 799 677 585 576 245 210 43 24 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5875 NdeI CA'TA_TG Cuts at: 387 387 Size: 6427 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6427 NotI GC'GGCC_GC Cuts at: 3874 3874 Size: 6427 NspI r_CATG'y Cuts at: 1132 1229 3435 3476 3785 5330 1132 Size: 97 2206 41 309 1545 2229 Fragments arranged by size: 2229 2206 1545 309 97 41 PasI CC'CwG_GG Cuts at: 1348 2212 3203 1348 Size: 864 991 4572 Fragments arranged by size: 4572 991 864 PciI A'CATG_T Cuts at: 3431 5326 3431 Size: 1895 4532 PflMI CCAn_nnn'nTGG Cuts at: 1108 2260 2381 1108 Size: 1152 121 5154 Fragments arranged by size: 5154 1152 121 PfoI T'CCnGG_A Cuts at: 2196 2944 3385 2196 Size: 748 441 5238 Fragments arranged by size: 5238 748 441 PmeI GTTT'AAAC Cuts at: 3731 3731 Size: 6427 PmlI CAC'GTG Cuts at: 3491 3491 Size: 6427 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4496 4528 6009 6041 4496 Size: 32 1481 32 4882 Fragments arranged by size: 4882 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2119 2909 3128 3544 3580 3617 2119 Size: 790 219 416 36 37 4929 Fragments arranged by size: 4929 790 416 219 37 36 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6427 PsiI TTA'TAA Cuts at: 3997 3997 Size: 6427 PspOMI G'GGCC_C Cuts at: 2130 2520 2130 Size: 390 6037 PstI C_TGCA'G Cuts at: 830 2190 2291 3779 830 Size: 1360 101 1488 3478 Fragments arranged by size: 3478 1488 1360 101 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6427 PvuII CAG'CTG Cuts at: 2182 2416 2459 3044 2182 Size: 234 43 585 5565 Fragments arranged by size: 5565 585 234 43 SalI G'TCGA_C Cuts at: 1936 3769 1936 Size: 1833 4594 SanDI GG'GwC_CC Cuts at: 3617 3617 Size: 6427 SbfI CC_TGCA'GG Cuts at: 3779 3779 Size: 6427 ScaI AGT'ACT Cuts at: 2599 4675 6387 2599 Size: 2076 1712 2639 Fragments arranged by size: 2639 2076 1712 SfcI C'TryA_G Cuts at: 826 1045 2186 2287 3775 4910 5591 5782 826 Size: 219 1141 101 1488 1135 681 191 1471 Fragments arranged by size: 1488 1471 1141 1135 681 219 191 101 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6427 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6427 SmaI CCC'GGG Cuts at: 1872 2519 3754 1872 Size: 647 1235 4545 Fragments arranged by size: 4545 1235 647 SmlI C'TyrA_G Cuts at: 820 1017 1943 4531 5432 5694 820 Size: 197 926 2588 901 262 1553 Fragments arranged by size: 2588 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4218 493 Size: 3725 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6427 SphI G_CATG'C Cuts at: 1132 3785 1132 Size: 2653 3774 SrfI GCCC'GGGC Cuts at: 2519 2519 Size: 6427 SspI AAT'ATT Cuts at: 5 52 4351 5 Size: 47 4299 2081 Fragments arranged by size: 4299 2081 47 StuI AGG'CCT Cuts at: 1517 2054 1517 Size: 537 5890 StyI C'CwwG_G Cuts at: 513 1065 3866 513 Size: 552 2801 3074 Fragments arranged by size: 3074 2801 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2627 4454 4471 4624 4809 5254 325 Size: 1255 1047 1827 17 153 185 445 1498 Fragments arranged by size: 1827 1498 1255 1047 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2597 4673 6385 Size: 274 80 33 51 1443 620 2076 1712 Cuts at: 6385 96 Size: 138 Fragments arranged by size: 2076 1712 1443 620 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2330 2362 2679 2711 3144 3176 6387 6419 2330 Size: 32 317 32 433 32 3211 32 2338 Fragments arranged by size: 3211 2338 433 317 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2486 1316 Size: 1170 5257 XbaI T'CTAG_A Cuts at: 3763 3763 Size: 6427 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6427 XmaI C'CCGG_G Cuts at: 1870 2517 3752 1870 Size: 647 1235 4545 Fragments arranged by size: 4545 1235 647 XmnI GAAnn'nnTTC Cuts at: 3443 4556 3443 Size: 1113 5314 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4235 276 Size: 53 83 186 936 2701 2468 Fragments arranged by size: 2701 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspXI PsrI RsrII SacI SacII SapI SexAI SfiI SfoI SwaI XcmI