(Circular) (Six-base) MAPSORT of: pFN21AB5786.seq Check: 3348 from: 1 to: 6892 pFN21AB5786 6892 bp With 182 enzymes: SgfI * April 15, 2010 09:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4702 278 Size: 53 83 186 936 3166 2468 Fragments arranged by size: 3166 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2293 2834 4235 1937 Size: 356 541 1401 4594 Fragments arranged by size: 4594 1401 541 356 Acc65I G'GTAC_C Cuts at: 4213 4213 Size: 6892 AclI AA'CG_TT Cuts at: 1563 2860 5019 5392 1563 Size: 1297 2159 373 3063 Fragments arranged by size: 3063 2159 1297 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2290 3093 4955 6339 6607 1632 Size: 658 803 1862 1384 268 1917 Fragments arranged by size: 1917 1862 1384 803 658 268 AfeI AGC'GCT Cuts at: 4016 4016 Size: 6892 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6695 AflIII A'CryG_T Cuts at: 1203 2058 2634 2829 5791 6768 1203 Size: 855 576 195 2962 977 1327 Fragments arranged by size: 2962 1327 977 855 576 195 AgeI A'CCGG_T Cuts at: 5723 5723 Size: 6892 AhdI GACnn_n'nnGTC Cuts at: 5621 5621 Size: 6892 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6892 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6892 AlwNI CAG_nnn'CTG Cuts at: 2666 3500 4635 6207 2666 Size: 834 1135 1572 3351 Fragments arranged by size: 3351 1572 1135 834 ApaLI G'TGCA_C Cuts at: 4949 6105 4949 Size: 1156 5736 ApoI r'AATT_y Cuts at: 1493 2200 2477 2609 2817 3036 3351 4201 4427 Size: 707 277 132 208 219 315 850 226 Cuts at: 4427 4579 6688 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 850 707 315 277 226 219 208 152 132 AseI AT'TA_AT Cuts at: 160 1032 5446 160 Size: 872 4414 1606 Fragments arranged by size: 4414 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6892 AvaI C'yCGr_G Cuts at: 1870 1943 3711 3947 4217 1870 Size: 73 1768 236 270 4545 Fragments arranged by size: 4545 1768 270 236 73 BamHI G'GATC_C Cuts at: 3943 4222 4589 3943 Size: 279 367 6246 Fragments arranged by size: 6246 367 279 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4213 5668 618 Size: 325 217 627 2426 1455 1842 Fragments arranged by size: 2426 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2116 2673 4211 1391 Size: 725 557 1538 4072 Fragments arranged by size: 4072 1538 725 557 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5921 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5083 5117 1903 Size: 34 3146 34 3678 Fragments arranged by size: 3678 3146 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3309 4784 5994 3309 Size: 1475 1210 4207 Fragments arranged by size: 4207 1475 1210 BclI T'GATC_A Cuts at: 1548 3601 1548 Size: 2053 4839 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5503 136 Size: 107 122 71 631 605 3831 1525 Fragments arranged by size: 3831 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2471 6887 2471 Size: 4416 2476 BlpI GC'TnA_GC Cuts at: 3864 4309 3864 Size: 445 6447 Bme1580I G_kGCm'C Cuts at: 1790 4050 4953 6109 1790 Size: 2260 903 1156 2573 Fragments arranged by size: 2573 2260 1156 903 BmgBI CAC'GTC Cuts at: 1318 2061 1318 Size: 743 6149 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4623 5581 442 Size: 416 53 83 399 24 3206 958 1753 Fragments arranged by size: 3206 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6892 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3387 3597 3937 5552 1239 Size: 2148 210 340 1615 2579 Fragments arranged by size: 2579 2148 1615 340 210 Bpu10I CC'TnA_GC Cuts at: 2107 2667 3159 2107 Size: 560 492 5840 Fragments arranged by size: 5840 560 492 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2525 5017 5882 6180 2525 Size: 2492 865 298 3237 Fragments arranged by size: 3237 2492 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2529 5555 882 Size: 617 1030 3026 2219 Fragments arranged by size: 3026 2219 1030 617 BsaAI yAC'GTr Cuts at: 493 1204 4683 493 Size: 711 3479 2702 Fragments arranged by size: 3479 2702 711 BsaBI GATnn'nnATC Cuts at: 6886 6886 Size: 6892 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2077 3984 4699 5081 Size: 53 83 186 936 544 1907 715 382 Cuts at: 5081 5716 275 Size: 635 1451 Fragments arranged by size: 1907 1451 936 715 635 544 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4116 5325 5723 5997 6144 6555 4116 Size: 1209 398 274 147 411 4453 Fragments arranged by size: 4453 1209 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2406 2436 1178 Size: 30 165 30 1003 30 5634 Fragments arranged by size: 5634 1003 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3195 1186 Size: 2009 4883 BseYI C'CCAG_C Cuts at: 3755 6095 6591 3755 Size: 2340 496 4056 Fragments arranged by size: 4056 2340 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2163 3239 3663 4085 1163 Size: 573 427 1076 424 422 3970 Fragments arranged by size: 3970 1076 573 427 424 422 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4342 5103 6131 1540 Size: 275 186 2341 761 1028 2301 Fragments arranged by size: 2341 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2116 2673 4953 5038 6109 2116 Size: 557 2280 85 1071 2899 Fragments arranged by size: 2899 2280 1071 557 85 BsmI GAATG_Cn' Cuts at: 3430 4403 4496 3430 Size: 973 93 5826 Fragments arranged by size: 5826 973 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2116 2673 4050 4211 4953 5038 6109 Size: 399 326 557 1377 161 742 85 1071 Cuts at: 6109 1391 Size: 2174 Fragments arranged by size: 2174 1377 1071 742 557 399 326 161 85 BspEI T'CCGG_A Cuts at: 6555 6555 Size: 6892 BspHI T'CATG_A Cuts at: 2907 4781 6511 2907 Size: 1874 1730 3288 Fragments arranged by size: 3288 1874 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4247 844 Size: 3403 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2106 4779 1394 Size: 429 283 2673 3507 Fragments arranged by size: 3507 2673 429 283 BsrDI GCAATG_nn' Cuts at: 65 1248 2021 2611 3859 5387 5561 65 Size: 1183 773 590 1248 1528 174 1396 Fragments arranged by size: 1528 1396 1248 1183 773 590 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5536 5723 1700 Size: 110 3726 187 2869 Fragments arranged by size: 3726 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6892 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6892 BssSI C'ACGA_G Cuts at: 4061 4952 5964 4061 Size: 891 1012 4989 Fragments arranged by size: 4989 1012 891 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6892 BstBI TT'CG_AA Cuts at: 2361 5696 2361 Size: 3335 3557 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6892 BstXI CCAn_nnnn'nTGG Cuts at: 4043 4043 Size: 6892 BstYI r'GATC_y Cuts at: 1972 2471 3943 4222 4589 4974 4991 6443 6529 Size: 499 1472 279 367 385 17 1452 86 Cuts at: 6529 6541 6887 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1472 1452 499 385 367 346 279 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 3700 4036 513 Size: 552 2635 336 3369 Fragments arranged by size: 3369 2635 552 336 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6153 Fragments arranged by size: 6153 683 56 BtsI GCAGTG_nn' Cuts at: 3181 3321 3382 3541 3678 4073 4408 5202 5222 Size: 140 61 159 137 395 335 794 20 Cuts at: 5222 3181 Size: 4851 Fragments arranged by size: 4851 794 395 335 159 140 137 61 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6892 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6857 DraI TTT'AAA Cuts at: 2334 4196 2334 Size: 1862 5030 DraIII CAC_nnn'GTG Cuts at: 3217 3565 3217 Size: 348 6544 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5899 809 Size: 507 4583 1802 Fragments arranged by size: 4583 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3196 3703 4339 Size: 54 1425 50 275 18 1366 507 636 Cuts at: 4339 5228 6857 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1366 889 636 507 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4339 1537 Size: 275 2527 4090 Fragments arranged by size: 4090 2527 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2309 2678 4031 4822 1353 Size: 956 369 1353 791 3423 Fragments arranged by size: 3423 1353 956 791 369 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3461 5479 5853 5999 387 Size: 1064 138 1872 2018 374 146 1280 Fragments arranged by size: 2018 1872 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2114 2671 2114 Size: 557 6335 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2290 3093 3387 3597 3937 4955 5552 Size: 393 658 803 294 210 340 1018 597 Cuts at: 5552 6339 6607 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1018 803 787 658 597 393 340 294 268 210 EcoO109I rG'GnC_Cy Cuts at: 2016 2016 Size: 6892 EcoRI G'AATT_C Cuts at: 2817 4201 4579 2817 Size: 1384 378 5130 Fragments arranged by size: 5130 1384 378 EcoRV GAT'ATC Cuts at: 4131 4131 Size: 6892 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3065 3249 3065 Size: 184 6708 FspI TGC'GCA Cuts at: 5398 5398 Size: 6892 HaeII r_GCGC'y Cuts at: 1678 4018 6039 1678 Size: 2340 2021 2531 Fragments arranged by size: 2531 2340 2021 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2320 2352 2515 2547 3163 Size: 32 135 32 748 32 163 32 616 Cuts at: 3163 3195 3545 3577 5533 5565 5607 5639 1373 Size: 32 350 32 1956 32 42 32 2626 Fragments arranged by size: 2626 1956 748 616 350 163 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4236 4482 669 Size: 988 281 2298 246 3079 Fragments arranged by size: 3079 2298 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6892 HpaI GTT'AAC Cuts at: 4482 4482 Size: 6892 KpnI G_GTAC'C Cuts at: 4217 4217 Size: 6892 MfeI C'AATT_G Cuts at: 4491 4491 Size: 6892 MluI A'CGCG_T Cuts at: 6768 6768 Size: 6892 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2309 3364 3960 6006 6190 1305 Size: 1004 1055 596 2046 184 2007 Fragments arranged by size: 2046 2007 1055 1004 596 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3198 10 Size: 54 1425 343 1366 3704 Fragments arranged by size: 3704 1425 1366 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2070 4041 4850 5209 5368 518 Size: 648 904 1971 809 359 159 2042 Fragments arranged by size: 2042 1971 904 809 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3551 4308 4985 6133 6378 1606 Size: 1945 757 677 1148 245 2120 Fragments arranged by size: 2120 1945 1148 757 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6340 NdeI CA'TA_TG Cuts at: 387 387 Size: 6892 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6892 NotI GC'GGCC_GC Cuts at: 4339 4339 Size: 6892 NspI r_CATG'y Cuts at: 1132 1229 2833 4250 5795 1132 Size: 97 1604 1417 1545 2229 Fragments arranged by size: 2229 1604 1545 1417 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6892 PciI A'CATG_T Cuts at: 2829 5791 2829 Size: 2962 3930 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6892 PfoI T'CCnGG_A Cuts at: 3359 3359 Size: 6892 PmeI GTTT'AAAC Cuts at: 4196 4196 Size: 6892 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4961 4993 6474 6506 4961 Size: 32 1481 32 5347 Fragments arranged by size: 5347 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2016 2016 Size: 6892 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6892 PsiI TTA'TAA Cuts at: 4462 4462 Size: 6892 PstI C_TGCA'G Cuts at: 830 2500 3328 4244 830 Size: 1670 828 916 3478 Fragments arranged by size: 3478 1670 916 828 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6892 PvuII CAG'CTG Cuts at: 3551 3551 Size: 6892 SacI G_AGCT'C Cuts at: 2116 2673 2116 Size: 557 6335 SalI G'TCGA_C Cuts at: 1936 4234 1936 Size: 2298 4594 SapI GCTCTTCn'nnn_ Cuts at: 2309 2678 4031 2309 Size: 369 1353 5170 Fragments arranged by size: 5170 1353 369 SbfI CC_TGCA'GG Cuts at: 4244 4244 Size: 6892 ScaI AGT'ACT Cuts at: 3193 5140 6852 3193 Size: 1947 1712 3233 Fragments arranged by size: 3233 1947 1712 SfcI C'TryA_G Cuts at: 826 1045 2130 2170 2496 2835 3256 3324 3546 Size: 219 1085 40 326 339 421 68 222 Cuts at: 3546 4240 5375 6056 6247 826 Size: 694 1135 681 191 1471 Fragments arranged by size: 1471 1135 1085 694 681 421 339 326 222 219 191 68 40 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6892 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6892 SmaI CCC'GGG Cuts at: 1872 3713 3949 4219 1872 Size: 1841 236 270 4545 Fragments arranged by size: 4545 1841 270 236 SmlI C'TyrA_G Cuts at: 820 1017 1943 2540 4996 5897 6159 820 Size: 197 926 597 2456 901 262 1553 Fragments arranged by size: 2456 1553 926 901 597 262 197 SnaBI TAC'GTA Cuts at: 493 4683 493 Size: 4190 2702 SpeI A'CTAG_T Cuts at: 152 2740 3932 152 Size: 2588 1192 3112 Fragments arranged by size: 3112 2588 1192 SphI G_CATG'C Cuts at: 1132 4250 1132 Size: 3118 3774 SspI AAT'ATT Cuts at: 5 52 4159 4816 5 Size: 47 4107 657 2081 Fragments arranged by size: 4107 2081 657 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6892 StyI C'CwwG_G Cuts at: 513 1065 4331 513 Size: 552 3266 3074 Fragments arranged by size: 3266 3074 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4919 4936 5089 5274 5719 325 Size: 1255 3339 17 153 185 445 1498 Fragments arranged by size: 3339 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3191 5138 6850 Size: 274 80 33 51 1443 1214 1947 1712 Cuts at: 6850 96 Size: 138 Fragments arranged by size: 1947 1712 1443 1214 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3355 3387 6852 6884 3355 Size: 32 3465 32 3363 Fragments arranged by size: 3465 3363 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6892 XbaI T'CTAG_A Cuts at: 2896 4228 2896 Size: 1332 5560 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2063 3937 2063 Size: 1874 5018 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6892 XmaI C'CCGG_G Cuts at: 1870 3711 3947 4217 1870 Size: 1841 236 270 4545 Fragments arranged by size: 4545 1841 270 236 XmnI GAAnn'nnTTC Cuts at: 2357 2850 3036 3106 3229 5021 2357 Size: 493 186 70 123 1792 4228 Fragments arranged by size: 4228 1792 493 186 123 70 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4700 276 Size: 53 83 186 936 3166 2468 Fragments arranged by size: 3166 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI