################################## # NO CUT ENZYMES ( 214 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcsI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BalI BanII BbeI BbrPI BbsI BbvCI BbvI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaJI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsmAI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BsrI BssHII BssKI BssSI Bst1107I BstAPI BstBI BstF5I BstNI BstUI BstXI BstZ17I Bsu36I Cac8I CelII Cfr10I ClaI CpoI DraI DraII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoO109I EcoRI EcoRII EcoRV EcoT14I EcoT22I FauI FbaI Fnu4HI FokI FseI FspI HaeIII HapII HgaI Hin1I HinfI HpaI HpaII ItaI KasI KpnI Ksp632I KspI MaeI MaeII MamI MfeI MluI MluNI MroI MscI MslI MspA1I MspI MunI MvaI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NlaIII NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI ScrFI SexAI SfaNI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI TfiI TseI Tsp509I TspRI Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 34 enzymes) # ################################### AspHI 181 GAGCAC G[AT]GC[AT]^C AvaII 223 GGACC G^G[AT]CC BamHI 138 GGATCC G^GATCC BanI 102 GGCACC G^G[CT][GA]CC BpmI 256 CTGGAG CTGGAG(16/14) BsiHKAI 181 GAGCAC G[AT]GC[AT]^C BsiYI 186 CCTTGTGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 186 CCTTGTGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmFI 222 GGGAC GGGAC(10/14) BstEII 27 GGTGACC G^GT[ACGT]ACC BstPI 27 GGTGACC G^GT[ACGT]ACC BstYI 138 GGATCC [GA]^GATC[CT] CfoI 262 GCGC GCG^C Cfr13I 223 GGACC G^G[ACGT]CC DdeI 199 CTAAG C^T[ACGT]AG DpnII 139 GATC ^GATC EcoNI 186 CCTTGTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 27 GGTGACC G^GT[ACGT]ACC HaeII 261 GGCGCT [GA]GCGC^[CT] HhaI 262 GCGC GCG^C HinP1I 262 GCGC G^CGC HincII 91 GTTGAC GT[CT]^[GA]AC HindII 91 GTTGAC GT[CT]^[GA]AC HindIII 148 AAGCTT A^AGCTT HphI 27 GGTGA GGTGA(8/7) MboI 139 GATC ^GATC MflI 138 GGATCC [GA]^GATC[CT] MseI 145 TTAA T^TAA NdeII 139 GATC ^GATC Sau3AI 139 GATC ^GATC Sau96I 223 GGACC G^G[ACGT]CC Tru9I 145 TTAA T^TAA Tsp45I 28 GTGAC ^GT[GC]AC XhoII 138 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 5 enzymes) # ################################### AlwI 139 GATCC GGATC(4/5) 138 GGATC BmyI 79 GTGCCC G[AGT]GC[ACT]^C 181 GAGCAC Bsp1286I 79 GTGCCC G[AGT]GC[ACT]^C 181 GAGCAC DraIII 184 CACCTTGTG CAC[ACGT][ACGT][ACGT]^GTG 271 CACTTGGTG MwoI 232 GCTTCTGAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 255 GCTGGAGGCGC ######################################### # 3 OR MORE CUT ENZYMES ( 5 enzymes) # ######################################### AluI MaeIII MboII MnlI NlaIV