################################## # NO CUT ENZYMES ( 165 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BcgI BciVI BclI BfrI BglI BglII BlnI BsaAI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsmBI BsmFI BsmI BsoBI BspDI BspEI BspHI BspMI BsrBI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstNI BstUI BstYI BstZ17I Bsu36I CfoI Cfr10I ClaI CpoI DraI DraII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoRI EcoRII EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HaeII HaeIII HgaI HhaI Hin1I HinP1I HincII HindII HpaI KasI KpnI KspI MaeII MfeI MflI MluI MluNI MroI MscI MunI MvaI MvnI NaeI NarI NcoI NgoMIV NheI NlaIV NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I StuI StyI SwaI TaqI Tth111I TthHB8I Van91I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 57 enzymes) # ################################### AcsI 883 AAATTT [GA]^AATT[CT] AflIII 692 ACATGT A^C[GA][CT]GT AlwI 714 GGATC GGATC(4/5) ApoI 883 AAATTT [GA]^AATT[CT] AseI 13 ATTAAT AT^TAAT AsnI 13 ATTAAT AT^TAAT AspHI 688 GAGCAC G[AT]GC[AT]^C AvaII 63 GGTCC G^G[AT]CC BbsI 700 GAAGAC GAAGAC(2/6) BbvCI 799 GCTGAGG CCTCAGC(-5/-2) BcnI 60 CCCGG CC^[GC]GG BlpI 416 GCTCAGC GC^T[ACGT]AGC BmyI 688 GAGCAC G[AGT]GC[ACT]^C BpmI 125 CTGGAG CTGGAG(16/14) Bpu1102I 416 GCTCAGC GC^T[ACGT]AGC BpuAI 700 GAAGAC GAAGAC(2/6) BsaBI 325 GATACATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaJI 59 CCCCGG C^C[ACGT][ACGT]GG BsiHKAI 688 GAGCAC G[AT]GC[AT]^C BsiYI 54 CCTGACCCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 54 CCTGACCCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmAI 823 GAGAC GTCTC(1/5) Bsp1286I 688 GAGCAC G[AGT]GC[ACT]^C Bsp1407I 888 TGTACA T^GTACA BspLU11I 692 ACATGT A^CATGT BsrDI 159 GCAATG GCAATG(2/0) BsrGI 888 TGTACA T^GTACA BssKI 60 CCCGG ^CC[ACGT]GG BstEII 222 GGTAACC G^GT[ACGT]ACC BstF5I 316 GGATG GGATG(2/0) BstPI 222 GGTAACC G^GT[ACGT]ACC BstXI 637 CCACGATTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 416 GCTCAGC GC^T[ACGT]AGC Cfr13I 63 GGTCC G^G[ACGT]CC DraIII 422 CACTATGTG CAC[ACGT][ACGT][ACGT]^GTG EcoO65I 222 GGTAACC G^GT[ACGT]ACC FokI 316 GGATG GGATG(9/13) HapII 61 CCGG C^CGG HindIII 539 AAGCTT A^AGCTT HpaII 61 CCGG C^CGG MamI 325 GATACATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MspA1I 122 CAGCTG C[AC]G^C[GT]G MspI 61 CCGG C^CGG NciI 60 CCCGG CC^[GC]GG NdeI 859 CATATG CA^TATG PleI 633 GACTC GAGTC(4/5) PshBI 13 ATTAAT AT^TAAT PstI 461 CTGCAG CTGCA^G PvuII 122 CAGCTG CAG^CTG Sau96I 63 GGTCC G^G[ACGT]CC ScrFI 60 CCCGG CC^[ACGT]GG SmlI 67 CTCAAG C^T[CT][GA]AG SphI 795 GCATGC GCATG^C SspBI 888 TGTACA T^GTACA TfiI 73 GAATC G^A[AT]TC Tsp45I 733 GTGAC ^GT[GC]AC XcmI 151 CCAACATGGCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 13 enzymes) # ################################### AfaI 51 GTAC GT^AC 889 GTAC BfaI 136 CTAG C^TAG 517 CTAG BsrI 36 ACTGG ACTGG(1/-1) 381 ACTGG HinfI 73 GAATC G^A[ACGT]TC 633 GACTC MaeI 136 CTAG C^TAG 517 CTAG MaeIII 223 GTAAC ^GT[ACGT]AC 733 GTGAC MwoI 541 GCTTGTAAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 666 GCAACGGCAGC NspI 692 ACATGT [GA]CATG^[CT] 795 GCATGC PshAI 57 GACCCCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 703 GACATCAGTC RsaI 51 GTAC GT^AC 889 GTAC SfaNI 559 GATGC GCATC(5/9) 574 GATGC SfcI 167 CTACAG C^T[GA][CT]AG 461 CTGCAG SspI 266 AATATT AAT^ATT 623 AATATT ######################################### # 3 OR MORE CUT ENZYMES ( 23 enzymes) # ######################################### AciI AluI BbvI Cac8I DdeI DpnII EarI Fnu4HI HphI ItaI Ksp632I MboI MboII MnlI MseI MslI NdeII NlaIII Sau3AI Tru9I TseI Tsp509I TspRI