################################## # NO CUT ENZYMES ( 141 enzymes) # ################################## AatII Acc65I AccII AclI AcyI AflII AgeI AhdI Alw44I ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BanI BbeI BbrPI BbvCI BcgI BfaI BfrI BglI BlnI BlpI Bpu1102I BsaBI BsaHI BsaI BseRI BsiEI BsiHKAI BsiWI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspHI BspMI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstZ17I CelII Cfr10I ClaI CpoI DraI DraIII DrdI EaeI EagI Eam1105I EarI EclXI Eco52I EcoO65I EcoRI EcoRV FseI FspI HgaI Hin1I HincII HindII HpaI KasI KpnI Ksp632I KspI MaeI MamI MfeI MluI MluNI MscI MunI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI SbfI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tth111I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AccI 370 GTCTAC GT^[AC][GT]AC AcsI 698 AAATTC [GA]^AATT[CT] AocI 448 CCTCAGG CC^T[ACGT]AGG Aor51HI 395 AGCGCT AGC^GCT ApaI 592 GGGCCC GGGCC^C ApoI 698 AAATTC [GA]^AATT[CT] BanII 592 GGGCCC G[GA]GC[CT]^C BciVI 585 GGATAC GTATCC(6/5) BclI 140 TGATCA T^GATCA BglII 306 AGATCT A^GATCT BmyI 592 GGGCCC G[AGT]GC[ACT]^C BsaAI 408 CACGTA [CT]AC^GT[GA] BsgI 625 CTGCAC GTGCAG(16/14) BsmAI 577 GTCTC GTCTC(1/5) Bsp1286I 592 GGGCCC G[AGT]GC[ACT]^C BsrBI 931 CCGCTC CCGCTC(-3/-3) BsrI 456 ACTGG ACTGG(1/-1) BstXI 705 CCATCACCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 448 CCTCAGG CC^T[ACGT]AGG CfoI 396 GCGC GCG^C DsaI 581 CCATGG C^C[GA][CT]GG Eco47III 395 AGCGCT AGC^GCT Eco81I 448 CCTCAGG CC^T[ACGT]AGG FauI 350 CCCGC CCCGC(4/6) FbaI 140 TGATCA T^GATCA HaeII 395 AGCGCT [GA]GCGC^[CT] HhaI 396 GCGC GCG^C HinP1I 396 GCGC G^CGC HindIII 619 AAGCTT A^AGCTT MaeII 409 ACGT A^CGT MspA1I 874 CAGCTG C[AC]G^C[GT]G NcoI 581 CCATGG C^CATGG PflMI 873 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 811 AGGTCCT [GA]G^G[AT]CC[CT] PvuII 874 CAGCTG CAG^CTG ScaI 215 AGTACT AGT^ACT SmlI 317 CTCAAG C^T[CT][GA]AG TaqI 153 TCGA T^CGA TspRI 144 CACTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 153 TCGA T^CGA Van91I 873 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AccIII 422 TCCGGA T^CCGGA 632 TCCGGA AfaI 216 GTAC GT^AC 411 GTAC AflIII 680 ACATGT A^C[GA][CT]GT 845 ACATGT Asp700I 175 GAATATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 498 GAAGGTCTTC BamHI 341 GGATCC G^GATCC 927 GGATCC BbsI 891 GAAGAC GAAGAC(2/6) 502 GTCTTC BcnI 186 CCCGG CC^[GC]GG 648 CCCGG BpuAI 891 GAAGAC GAAGAC(2/6) 502 GTCTTC BsaWI 422 TCCGGA [AT]^CCGG[AT] 632 TCCGGA BseAI 422 TCCGGA T^CCGGA 632 TCCGGA BspEI 422 TCCGGA T^CCGGA 632 TCCGGA BspLU11I 680 ACATGT A^CATGT 845 ACATGT BstAPI 604 GCAGGCGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 798 GCAGACCTTGC Cac8I 604 GCAGGC GC[ACGT]^[ACGT]GC 872 GCCAGC DdeI 402 CTCAG C^T[ACGT]AG 449 CTCAG EcoNI 45 CCTGAATCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 803 CCTTGCAGAGG EcoT22I 461 ATGCAT ATGCA^T 784 ATGCAT MroI 422 TCCGGA T^CCGGA 632 TCCGGA MseI 73 TTAA T^TAA 262 TTAA MslI 438 CATCTGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 568 CATGTCAATG NciI 186 CCCGG CC^[GC]GG 648 CCCGG NsiI 461 ATGCAT ATGCA^T 784 ATGCAT NspI 680 ACATGT [GA]CATG^[CT] 845 ACATGT PleI 286 GACTC GAGTC(4/5) 190 GAGTC RsaI 216 GTAC GT^AC 411 GTAC Tru9I 73 TTAA T^TAA 262 TTAA Tsp45I 530 GTGAC ^GT[GC]AC 686 GTCAC Tsp509I 514 AATT ^AATT 699 AATT XmnI 175 GAATATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 498 GAAGGTCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 47 enzymes) # ######################################### AciI AluI AlwI AlwNI AvaII BbvI BpmI BsaJI BsiYI BslI BssKI BstF5I BstNI BstYI Cfr13I DpnII DraII EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI MaeIII MboI MboII MflI MnlI MspI MvaI MwoI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI SfaNI StyI TfiI TseI XhoII