(Circular) (Six-base) MAPSORT of: pFN21AE5402.seq Check: 6614 from: 1 to: 5644 pFN21AE5402 5644 bp With 182 enzymes: SgfI * January 30, 2014 17:19 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3454 278 Size: 53 83 186 936 1918 2468 Fragments arranged by size: 2468 1918 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2376 2987 1937 Size: 439 611 4594 Fragments arranged by size: 4594 611 439 Acc65I G'GTAC_C Cuts at: 2965 2965 Size: 5644 AclI AA'CG_TT Cuts at: 1563 3771 4144 1563 Size: 2208 373 3063 Fragments arranged by size: 3063 2208 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3707 5091 5359 1632 Size: 2075 1384 268 1917 Fragments arranged by size: 2075 1917 1384 268 AfeI AGC'GCT Cuts at: 2402 2402 Size: 5644 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5447 AflIII A'CryG_T Cuts at: 1203 2685 2850 4543 5520 1203 Size: 1482 165 1693 977 1327 Fragments arranged by size: 1693 1482 1327 977 165 AgeI A'CCGG_T Cuts at: 4475 4475 Size: 5644 AhdI GACnn_n'nnGTC Cuts at: 4373 4373 Size: 5644 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5644 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5644 AlwNI CAG_nnn'CTG Cuts at: 2294 2461 2884 3387 4959 2294 Size: 167 423 503 1572 2979 Fragments arranged by size: 2979 1572 503 423 167 ApaI G_GGCC'C Cuts at: 2601 2601 Size: 5644 ApaLI G'TGCA_C Cuts at: 3701 4857 3701 Size: 1156 4488 ApoI r'AATT_y Cuts at: 1493 2703 2953 3179 3331 5440 1493 Size: 1210 250 226 152 2109 1697 Fragments arranged by size: 2109 1697 1210 250 226 152 AseI AT'TA_AT Cuts at: 160 1032 4198 160 Size: 872 3166 1606 Fragments arranged by size: 3166 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5644 AvaI C'yCGr_G Cuts at: 1870 1943 2969 1870 Size: 73 1026 4545 Fragments arranged by size: 4545 1026 73 BamHI G'GATC_C Cuts at: 2346 2932 2974 3341 2346 Size: 586 42 367 4649 Fragments arranged by size: 4649 586 367 42 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2965 4420 618 Size: 325 217 627 1178 1455 1842 Fragments arranged by size: 1842 1455 1178 627 325 217 BanII G_rGCy'C Cuts at: 1391 2601 2963 1391 Size: 1210 362 4072 Fragments arranged by size: 4072 1210 362 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2500 2903 928 Size: 971 601 403 3669 Fragments arranged by size: 3669 971 601 403 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3835 3869 1903 Size: 34 50 34 1814 34 3678 Fragments arranged by size: 3678 1814 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2584 3536 4746 2584 Size: 952 1210 3482 Fragments arranged by size: 3482 1210 952 BclI T'GATC_A Cuts at: 1548 2145 1548 Size: 597 5047 BfrBI ATG'CAT Cuts at: 2468 2791 2468 Size: 323 5321 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4255 136 Size: 107 122 71 631 605 2583 1525 Fragments arranged by size: 2583 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2311 5639 2311 Size: 3328 2316 BlpI GC'TnA_GC Cuts at: 3061 3061 Size: 5644 Bme1580I G_kGCm'C Cuts at: 1790 2601 3705 4861 1790 Size: 811 1104 1156 2573 Fragments arranged by size: 2573 1156 1104 811 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5644 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3375 4333 442 Size: 416 53 83 399 24 1958 958 1753 Fragments arranged by size: 1958 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5644 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2422 2422 Size: 5644 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2056 2228 2276 4304 1239 Size: 817 172 48 2028 2579 Fragments arranged by size: 2579 2028 817 172 48 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2307 3769 4634 4932 2307 Size: 1462 865 298 3019 Fragments arranged by size: 3019 1462 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4307 882 Size: 617 2808 2219 Fragments arranged by size: 2808 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2415 3435 493 Size: 711 1211 1020 2702 Fragments arranged by size: 2702 1211 1020 711 BsaBI GATnn'nnATC Cuts at: 5638 5638 Size: 5644 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3451 3833 4468 275 Size: 53 83 186 936 1918 382 635 1451 Fragments arranged by size: 1918 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2427 2637 4077 4475 4749 4896 5307 2427 Size: 210 1440 398 274 147 411 2764 Fragments arranged by size: 2764 1440 411 398 274 210 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5419 Fragments arranged by size: 5419 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5644 BseYI C'CCAG_C Cuts at: 4847 5343 4847 Size: 496 5148 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2615 1163 Size: 573 879 4192 Fragments arranged by size: 4192 879 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3094 3855 4883 1540 Size: 275 186 1093 761 1028 2301 Fragments arranged by size: 2301 1093 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3705 3790 4861 3705 Size: 85 1071 4488 Fragments arranged by size: 4488 1071 85 BsmI GAATG_Cn' Cuts at: 3155 3248 3155 Size: 93 5551 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2601 2963 3705 3790 4861 1391 Size: 399 811 362 742 85 1071 2174 Fragments arranged by size: 2174 1071 811 742 399 362 85 BspEI T'CCGG_A Cuts at: 2427 2637 5307 2427 Size: 210 2670 2764 Fragments arranged by size: 2764 2670 210 BspHI T'CATG_A Cuts at: 3533 5263 3533 Size: 1730 3914 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2999 844 Size: 2155 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2938 3531 1394 Size: 429 1115 593 3507 Fragments arranged by size: 3507 1115 593 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4139 4313 65 Size: 1183 2891 174 1396 Fragments arranged by size: 2891 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4288 4475 1700 Size: 110 2478 187 2869 Fragments arranged by size: 2869 2478 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5644 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5644 BssSI C'ACGA_G Cuts at: 3704 4716 3704 Size: 1012 4632 BstAPI GCAn_nnn'nTGC Cuts at: 794 2615 2809 794 Size: 1821 194 3629 Fragments arranged by size: 3629 1821 194 BstBI TT'CG_AA Cuts at: 4448 4448 Size: 5644 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5644 BstXI CCAn_nnnn'nTGG Cuts at: 2717 2717 Size: 5644 BstYI r'GATC_y Cuts at: 1972 2311 2346 2932 2974 3341 3726 3743 5195 Size: 339 35 586 42 367 385 17 1452 Cuts at: 5195 5281 5293 5639 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 586 385 367 346 339 86 42 35 17 12 Bsu36I CC'TnA_GG Cuts at: 2454 2454 Size: 5644 BtgI C'CryG_G Cuts at: 513 1065 2586 513 Size: 552 1521 3571 Fragments arranged by size: 3571 1521 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2628 487 Size: 683 56 1402 3503 Fragments arranged by size: 3503 1402 683 56 BtsI GCAGTG_nn' Cuts at: 2148 3160 3954 3974 2148 Size: 1012 794 20 3818 Fragments arranged by size: 3818 1012 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5644 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5609 DraI TTT'AAA Cuts at: 2948 2948 Size: 5644 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4651 809 Size: 507 3335 1802 Fragments arranged by size: 3335 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3091 3980 5609 Size: 54 1425 50 275 18 1261 889 1629 Cuts at: 5609 8 Size: 43 Fragments arranged by size: 1629 1425 1261 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3091 1537 Size: 275 1279 4090 Fragments arranged by size: 4090 1279 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3574 1353 Size: 2221 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4231 4605 4751 387 Size: 1064 138 2642 374 146 1280 Fragments arranged by size: 2642 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2056 2228 2276 3707 4304 5091 5359 Size: 393 424 172 48 1431 597 787 268 Cuts at: 5359 1239 Size: 1524 Fragments arranged by size: 1524 1431 787 597 424 393 268 172 48 EcoNI CCTnn'n_nnAGG Cuts at: 2054 2812 2054 Size: 758 4886 EcoO109I rG'GnC_Cy Cuts at: 2597 2598 2817 2597 Size: 1 219 5424 Fragments arranged by size: 5424 219 1 EcoRI G'AATT_C Cuts at: 2953 3331 2953 Size: 378 5266 FspI TGC'GCA Cuts at: 4150 4150 Size: 5644 HaeII r_GCGC'y Cuts at: 1678 2404 4791 1678 Size: 726 2387 2531 Fragments arranged by size: 2531 2387 726 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2276 2308 2901 2933 4285 Size: 32 135 32 704 32 593 32 1352 Cuts at: 4285 4317 4359 4391 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1352 704 593 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2988 3234 669 Size: 988 281 1050 246 3079 Fragments arranged by size: 3079 1050 988 281 246 HindIII A'AGCT_T Cuts at: 748 2624 748 Size: 1876 3768 HpaI GTT'AAC Cuts at: 3234 3234 Size: 5644 KpnI G_GTAC'C Cuts at: 2969 2969 Size: 5644 MfeI C'AATT_G Cuts at: 3243 3243 Size: 5644 MluI A'CGCG_T Cuts at: 5520 5520 Size: 5644 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4758 4942 1305 Size: 3453 184 2007 Fragments arranged by size: 3453 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3822 Fragments arranged by size: 3822 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2447 2577 3602 3961 4120 518 Size: 648 1281 130 1025 359 159 2042 Fragments arranged by size: 2042 1281 1025 648 359 159 130 MspA1I CmG'CkG Cuts at: 1606 2881 3060 3737 4885 5130 1606 Size: 1275 179 677 1148 245 2120 Fragments arranged by size: 2120 1275 1148 677 245 179 NcoI C'CATG_G Cuts at: 513 1065 2586 513 Size: 552 1521 3571 Fragments arranged by size: 3571 1521 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5644 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5644 NotI GC'GGCC_GC Cuts at: 3091 3091 Size: 5644 NsiI A_TGCA'T Cuts at: 2470 2793 2470 Size: 323 5321 NspI r_CATG'y Cuts at: 1132 1229 2689 2854 3002 4547 1132 Size: 97 1460 165 148 1545 2229 Fragments arranged by size: 2229 1545 1460 165 148 97 PasI CC'CwG_GG Cuts at: 1348 2746 1348 Size: 1398 4246 PciI A'CATG_T Cuts at: 2685 2850 4543 2685 Size: 165 1693 3786 Fragments arranged by size: 3786 1693 165 PflMI CCAn_nnn'nTGG Cuts at: 1108 2884 1108 Size: 1776 3868 PfoI T'CCnGG_A Cuts at: 2190 2190 Size: 5644 PmeI GTTT'AAAC Cuts at: 2948 2948 Size: 5644 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3713 3745 5226 5258 3713 Size: 32 1481 32 4099 Fragments arranged by size: 4099 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2817 2817 Size: 5644 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5644 PsiI TTA'TAA Cuts at: 3214 3214 Size: 5644 PspOMI G'GGCC_C Cuts at: 2597 2597 Size: 5644 PstI C_TGCA'G Cuts at: 830 2996 830 Size: 2166 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5644 PvuII CAG'CTG Cuts at: 2881 2881 Size: 5644 SalI G'TCGA_C Cuts at: 1936 2986 1936 Size: 1050 4594 SbfI CC_TGCA'GG Cuts at: 2996 2996 Size: 5644 ScaI AGT'ACT Cuts at: 2222 3892 5604 2222 Size: 1670 1712 2262 Fragments arranged by size: 2262 1712 1670 SfcI C'TryA_G Cuts at: 826 1045 2992 4127 4808 4999 826 Size: 219 1947 1135 681 191 1471 Fragments arranged by size: 1947 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5644 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5644 SmaI CCC'GGG Cuts at: 1872 2971 1872 Size: 1099 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2322 3748 4649 4911 820 Size: 197 926 379 1426 901 262 1553 Fragments arranged by size: 1553 1426 926 901 379 262 197 SnaBI TAC'GTA Cuts at: 493 3435 493 Size: 2942 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5644 SphI G_CATG'C Cuts at: 1132 3002 1132 Size: 1870 3774 SspI AAT'ATT Cuts at: 5 52 3568 5 Size: 47 3516 2081 Fragments arranged by size: 3516 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5644 StyI C'CwwG_G Cuts at: 513 1065 2586 2716 2905 3083 513 Size: 552 1521 130 189 178 3074 Fragments arranged by size: 3074 1521 552 189 178 130 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2378 2759 3671 3688 3841 4026 4471 Size: 1255 798 381 912 17 153 185 445 Cuts at: 4471 325 Size: 1498 Fragments arranged by size: 1498 1255 912 798 445 381 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2220 3890 5602 Size: 274 80 33 51 1443 243 1670 1712 Cuts at: 5602 96 Size: 138 Fragments arranged by size: 1712 1670 1443 274 243 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2195 2227 5604 5636 2195 Size: 32 3377 32 2203 Fragments arranged by size: 3377 2203 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5644 XbaI T'CTAG_A Cuts at: 2980 2980 Size: 5644 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5644 XmaI C'CCGG_G Cuts at: 1870 2969 1870 Size: 1099 4545 XmnI GAAnn'nnTTC Cuts at: 2184 2507 3773 2184 Size: 323 1266 4055 Fragments arranged by size: 4055 1266 323 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3452 276 Size: 53 83 186 936 1918 2468 Fragments arranged by size: 2468 1918 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbeI BbvCI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI