(Linear) (Six-base) MAPSORT of: KIEE5402.seq Check: 2316 from: 1 to: 939 KIEE5402.orf With 182 enzymes: SgfI * January 29, 2014 11:58 .. AccI GT'mk_AC Cuts at: 0 371 939 Size: 371 568 AfeI AGC'GCT Cuts at: 0 397 939 Size: 397 542 AflIII A'CryG_T Cuts at: 0 680 845 939 Size: 680 165 94 Fragments arranged by size: 680 165 94 AlwNI CAG_nnn'CTG Cuts at: 0 289 456 879 939 Size: 289 167 423 60 Fragments arranged by size: 423 289 167 60 ApaI G_GGCC'C Cuts at: 0 596 939 Size: 596 343 ApoI r'AATT_y Cuts at: 0 698 939 Size: 698 241 BamHI G'GATC_C Cuts at: 0 341 927 939 Size: 341 586 12 Fragments arranged by size: 586 341 12 BanII G_rGCy'C Cuts at: 0 596 939 Size: 596 343 BbsI GAAGACnn'nnnn_ Cuts at: 0 495 898 939 Size: 495 403 41 Fragments arranged by size: 495 403 41 BciVI GTATCCnnnnn_n' Cuts at: 0 579 939 Size: 579 360 BclI T'GATC_A Cuts at: 0 140 939 Size: 140 799 BfrBI ATG'CAT Cuts at: 0 463 786 939 Size: 463 323 153 Fragments arranged by size: 463 323 153 BglII A'GATC_T Cuts at: 0 306 939 Size: 306 633 Bme1580I G_kGCm'C Cuts at: 0 596 939 Size: 596 343 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 417 939 Size: 417 522 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 51 223 271 939 Size: 51 172 48 668 Fragments arranged by size: 668 172 51 48 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 302 939 Size: 302 637 BsaAI yAC'GTr Cuts at: 0 410 939 Size: 410 529 BsaWI w'CCGG_w Cuts at: 0 422 632 939 Size: 422 210 307 Fragments arranged by size: 422 307 210 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 610 939 Size: 610 329 Bsp1286I G_dGCh'C Cuts at: 0 596 939 Size: 596 343 BspEI T'CCGG_A Cuts at: 0 422 632 939 Size: 422 210 307 Fragments arranged by size: 422 307 210 BsrBI CCG'CTC Cuts at: 0 933 939 Size: 933 6 BstAPI GCAn_nnn'nTGC Cuts at: 0 610 804 939 Size: 610 194 135 Fragments arranged by size: 610 194 135 BstXI CCAn_nnnn'nTGG Cuts at: 0 712 939 Size: 712 227 BstYI r'GATC_y Cuts at: 0 306 341 927 939 Size: 306 35 586 12 Fragments arranged by size: 586 306 35 12 Bsu36I CC'TnA_GG Cuts at: 0 449 939 Size: 449 490 BtgI C'CryG_G Cuts at: 0 581 939 Size: 581 358 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 623 939 Size: 623 316 BtsI GCAGTG_nn' Cuts at: 0 143 939 Size: 143 796 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 51 223 271 939 Size: 51 172 48 668 Fragments arranged by size: 668 172 51 48 EcoNI CCTnn'n_nnAGG Cuts at: 0 49 807 939 Size: 49 758 132 Fragments arranged by size: 758 132 49 EcoO109I rG'GnC_Cy Cuts at: 0 592 593 812 939 Size: 592 1 219 127 Fragments arranged by size: 592 219 127 1 HaeII r_GCGC'y Cuts at: 0 399 939 Size: 399 540 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 271 303 896 928 939 Size: 271 32 593 32 11 Fragments arranged by size: 593 271 32 32 11 HindIII A'AGCT_T Cuts at: 0 619 939 Size: 619 320 MslI CAynn'nnrTG Cuts at: 0 442 572 939 Size: 442 130 367 Fragments arranged by size: 442 367 130 MspA1I CmG'CkG Cuts at: 0 876 939 Size: 876 63 NcoI C'CATG_G Cuts at: 0 581 939 Size: 581 358 NsiI A_TGCA'T Cuts at: 0 465 788 939 Size: 465 323 151 Fragments arranged by size: 465 323 151 NspI r_CATG'y Cuts at: 0 684 849 939 Size: 684 165 90 Fragments arranged by size: 684 165 90 PasI CC'CwG_GG Cuts at: 0 741 939 Size: 741 198 PciI A'CATG_T Cuts at: 0 680 845 939 Size: 680 165 94 Fragments arranged by size: 680 165 94 PflMI CCAn_nnn'nTGG Cuts at: 0 879 939 Size: 879 60 PfoI T'CCnGG_A Cuts at: 0 185 939 Size: 185 754 PpuMI rG'GwC_Cy Cuts at: 0 812 939 Size: 812 127 PspOMI G'GGCC_C Cuts at: 0 592 939 Size: 592 347 PvuII CAG'CTG Cuts at: 0 876 939 Size: 876 63 ScaI AGT'ACT Cuts at: 0 217 939 Size: 217 722 SmlI C'TyrA_G Cuts at: 0 317 939 Size: 317 622 StyI C'CwwG_G Cuts at: 0 581 711 900 939 Size: 581 130 189 39 Fragments arranged by size: 581 189 130 39 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 373 754 939 Size: 373 381 185 Fragments arranged by size: 381 373 185 TatI w'GTAC_w Cuts at: 0 215 939 Size: 215 724 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 190 222 939 Size: 190 32 717 Fragments arranged by size: 717 190 32 XmnI GAAnn'nnTTC Cuts at: 0 179 502 939 Size: 179 323 437 Fragments arranged by size: 437 323 179 Enzymes that do cut: AccI AfeI AflIII AlwNI ApaI ApoI BamHI BanII BbsI BciVI BclI BfrBI BglII Bme1580I BplI BpmI BpuEI BsaAI BsaWI BsgI Bsp1286I BspEI BsrBI BstAPI BstXI BstYI Bsu36I BtgI BtgZI BtsI Eco57MI EcoNI EcoO109I HaeII Hin4I HindIII MslI MspA1I NcoI NsiI NspI PasI PciI PflMI PfoI PpuMI PspOMI PvuII ScaI SmlI StyI TaqII TatI TstI XmnI Enzymes that do not cut: AarI AatII Acc65I AclI AcuI AflII AgeI AhdI AleI AlfI AloI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BanI BbeI BbvCI BcgI BglI BlpI BmgBI BmrI BmtI Bpu10I BsaI BsaBI BsaHI BsaXI BseRI BseYI BsiEI BsiHKAI BsiWI BsmI BsmBI BspHI BspMI BsrDI BsrFI BsrGI BssHII BssSI BstBI BstEII BstZ17I ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI HincII HpaI KasI KpnI MfeI MluI MmeI MscI NaeI NarI NdeI NgoMIV NheI NotI NruI PacI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PstI PvuI RsrII SacI SacII SalI SanDI SapI SbfI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI Tth111I XbaI XcmI XhoI XmaI ZraI