################################## # NO CUT ENZYMES ( 102 enzymes) # ################################## Acc65I AccI AclI AcsI AflII AflIII AgeI AhdI AocI ApoI AscI AseI AsnI Asp718I AspEI AviII AvrII BbsI BcgI BciVI BclI BfaI BfrI BglII BlnI BpuAI BsiWI Bsp1407I BspDI BspHI BspLU11I BspMI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I ClaI CpoI DraI DrdI EagI Eam1105I EclXI Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HindIII HpaI KpnI MaeI MfeI MluI MseI MunI NcoI NdeI NheI NotI NruI NsiI NspI NspV PacI PflMI PinAI PmeI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SalI ScaI SfuI SmaI SnaBI SpeI SphI SspBI SspI StuI SwaI Tru9I Tsp509I Van91I XbaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 60 enzymes) # ################################### AatII 637 GACGTC GACGT^C AccIII 818 TCCGGA T^CCGGA Alw44I 1141 GTGCAC G^TGCAC AlwNI 536 CAGCGGCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 824 AGCGCT AGC^GCT ApaI 108 GGGCCC GGGCC^C ApaLI 1141 GTGCAC G^TGCAC Asp700I 924 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 33 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 48 CTCGAG C^[CT]CG[GA]G BbeI 23 GGCGCC GGCGC^C BbrPI 502 CACGTG CAC^GTG BbvCI 195 GCTGAGG CCTCAGC(-5/-2) BlpI 1048 GCTGAGC GC^T[ACGT]AGC Bpu1102I 1048 GCTGAGC GC^T[ACGT]AGC BsaBI 282 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 1067 GAGACC GGTCTC(1/5) BsaWI 818 TCCGGA [AT]^CCGG[AT] BseAI 818 TCCGGA T^CCGGA BseRI 85 GAGGAG GAGGAG(10/8) BsgI 499 CTGCAC GTGCAG(16/14) BsiEI 6 CGACCG CG[GA][CT]^CG BsmBI 164 GAGACG CGTCTC(1/5) BsoBI 48 CTCGAG C^[CT]CG[GA]G BspEI 818 TCCGGA T^CCGGA BsrBI 804 CCGCTC CCGCTC(-3/-3) BsrDI 296 GCAATG GCAATG(2/0) BsrFI 169 GCCGGC [GA]^CCGG[CT] BssSI 1243 CACGAG CACGAG(-5/-1) CelII 1048 GCTGAGC GC^T[ACGT]AGC Cfr10I 169 GCCGGC [GA]^CCGG[CT] Eco47III 824 AGCGCT AGC^GCT EcoT14I 668 CCAAGG C^C[AT][AT]GG HincII 640 GTCAAC GT[CT]^[GA]AC HindII 640 GTCAAC GT[CT]^[GA]AC KasI 23 GGCGCC G^GCGCC MaeIII 1108 GTCAC ^GT[ACGT]AC MamI 282 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 818 TCCGGA T^CCGGA NaeI 169 GCCGGC GCC^GGC NarI 23 GGCGCC GG^CGCC NgoMIV 169 GCCGGC G^CCGGC PaeR7I 48 CTCGAG C^TCGAG PflFI 33 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 502 CACGTG CAC^GTG PmlI 502 CACGTG CAC^GTG PvuII 1364 CAGCTG CAG^CTG SbfI 210 CCTGCAGG CCTGCA^GG SexAI 1250 ACCTGGT A^CC[AT]GGT SfiI 507 GGCCAACCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 23 GGCGCC GGC^GCC SgrAI 168 CGCCGGCG C[GA]^CCGG[CT]G Sse8387I 210 CCTGCAGG CCTGCA^GG StyI 668 CCAAGG C^C[AT][AT]GG TfiI 217 GATTC G^A[AT]TC Tsp45I 1108 GTCAC ^GT[GC]AC Tth111I 33 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 1344 CCAGGGCAACTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 48 CTCGAG C^TCGAG XmnI 924 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AfaI 1248 GTAC GT^AC 1413 GTAC BamHI 378 GGATCC G^GATCC 842 GGATCC BsaAI 502 CACGTG [CT]AC^GT[GA] 1429 TACGTG BsmAI 164 GAGAC GTCTC(1/5) 1067 GAGAC BsmI 1038 GAATGC GAATGC(1/-1) 439 GCATTC BstXI 350 CCATCCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1315 CCACTGGCCTGG DraII 108 GGGCCCC [GA]G^G[ACGT]CC[CT] 1445 AGGCCCC DraIII 715 CACACCGTG CAC[ACGT][ACGT][ACGT]^GTG 777 CACCTTGTG EcoO109I 108 GGGCCCC [GA]G^G[ACGT]CC[CT] 1445 AGGCCCC KspI 9 CCGCGG CCGC^GG 125 CCGCGG PleI 559 GAGTC GAGTC(4/5) 1102 GAGTC PstI 211 CTGCAG CTGCA^G 680 CTGCAG RsaI 1248 GTAC GT^AC 1413 GTAC SacI 790 GAGCTC GAGCT^C 1051 GAGCTC SacII 9 CCGCGG CCGC^GG 125 CCGCGG SapI 621 GCTCTTC GCTCTTC(1/4) 1020 GCTCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 80 enzymes) # ######################################### AccII AciI AcyI AluI AlwI AspHI AvaII BalI BanI BanII BbvI BcnI BglI BmyI BpmI BsaHI BsaJI BsiHKAI BsiYI BslI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DsaI EaeI EarI EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeII MboI MboII MflI MluNI MnlI MscI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI SfaNI SfcI SmlI TaqI TseI TspRI TthHB8I XhoII