(Linear) (Six-base) MAPSORT of: KIEE4466.seq Check: 8248 from: 1 to: 1464 KIEE4466.orf With 182 enzymes: SgfI * March 22, 2012 18:07 .. AatII G_ACGT'C Cuts at: 0 641 1464 Size: 641 823 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 228 394 991 1464 Size: 228 166 597 473 Fragments arranged by size: 597 473 228 166 AfeI AGC'GCT Cuts at: 0 826 1464 Size: 826 638 AleI CACnn'nnGTG Cuts at: 0 571 1464 Size: 571 893 AlwNI CAG_nnn'CTG Cuts at: 0 541 1464 Size: 541 923 ApaI G_GGCC'C Cuts at: 0 112 1464 Size: 112 1352 ApaLI G'TGCA_C Cuts at: 0 1141 1464 Size: 1141 323 AvaI C'yCGr_G Cuts at: 0 48 1464 Size: 48 1416 BamHI G'GATC_C Cuts at: 0 378 842 1464 Size: 378 464 622 Fragments arranged by size: 622 464 378 BanI G'GyrC_C Cuts at: 0 23 200 469 650 1464 Size: 23 177 269 181 814 Fragments arranged by size: 814 269 181 177 23 BanII G_rGCy'C Cuts at: 0 112 580 794 1055 1464 Size: 112 468 214 261 409 Fragments arranged by size: 468 409 261 214 112 BbeI G_GCGC'C Cuts at: 0 27 1464 Size: 27 1437 BbvCI CC'TCA_GC Cuts at: 0 196 1464 Size: 196 1268 BglI GCCn_nnn'nGGC Cuts at: 0 59 514 1453 1464 Size: 59 455 939 11 Fragments arranged by size: 939 455 59 11 BlpI GC'TnA_GC Cuts at: 0 1049 1464 Size: 1049 415 Bme1580I G_kGCm'C Cuts at: 0 112 474 1145 1464 Size: 112 362 671 319 Fragments arranged by size: 671 362 319 112 BmgBI CAC'GTC Cuts at: 0 117 1464 Size: 117 1347 BmrI ACTGGGnnnn_n' Cuts at: 0 198 1464 Size: 198 1266 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1280 1464 Size: 1280 184 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 334 554 856 1464 Size: 334 220 302 608 Fragments arranged by size: 608 334 302 220 Bpu10I CC'TnA_GC Cuts at: 0 196 1464 Size: 196 1268 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 312 601 868 1464 Size: 312 289 267 596 Fragments arranged by size: 596 312 289 267 BsaI GGTCTCn'nnnn_ Cuts at: 0 1061 1464 Size: 1061 403 BsaAI yAC'GTr Cuts at: 0 504 1431 1464 Size: 504 927 33 Fragments arranged by size: 927 504 33 BsaBI GATnn'nnATC Cuts at: 0 286 1464 Size: 286 1178 BsaHI Gr'CG_yC Cuts at: 0 24 98 167 638 1464 Size: 24 74 69 471 826 Fragments arranged by size: 826 471 74 69 24 BsaWI w'CCGG_w Cuts at: 0 818 1464 Size: 818 646 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 184 214 1464 Size: 184 30 1250 Fragments arranged by size: 1250 184 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 100 1464 Size: 100 1364 BseYI C'CCAG_C Cuts at: 0 534 866 1449 1464 Size: 534 332 583 15 Fragments arranged by size: 583 534 332 15 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 484 1464 Size: 484 980 BsiEI CG_ry'CG Cuts at: 0 9 1464 Size: 9 1455 BsiHKAI G_wGCw'C Cuts at: 0 794 1055 1145 1436 1464 Size: 794 261 90 291 28 Fragments arranged by size: 794 291 261 90 28 BsmI GAATG_Cn' Cuts at: 0 439 1044 1464 Size: 439 605 420 Fragments arranged by size: 605 439 420 BsmBI CGTCTCn'nnnn_ Cuts at: 0 158 1464 Size: 158 1306 Bsp1286I G_dGCh'C Cuts at: 0 112 474 580 794 1055 1145 1436 1464 Size: 112 362 106 214 261 90 291 28 Fragments arranged by size: 362 291 261 214 112 106 90 28 BspEI T'CCGG_A Cuts at: 0 818 1464 Size: 818 646 BsrBI CCG'CTC Cuts at: 0 806 1464 Size: 806 658 BsrDI GCAATG_nn' Cuts at: 0 303 1464 Size: 303 1161 BsrFI r'CCGG_y Cuts at: 0 169 1464 Size: 169 1295 BssSI C'ACGA_G Cuts at: 0 1243 1464 Size: 1243 221 BstXI CCAn_nnnn'nTGG Cuts at: 0 357 1322 1464 Size: 357 965 142 Fragments arranged by size: 965 357 142 BstYI r'GATC_y Cuts at: 0 378 842 1310 1464 Size: 378 464 468 154 Fragments arranged by size: 468 464 378 154 BtgI C'CryG_G Cuts at: 0 9 54 125 255 1043 1464 Size: 9 45 71 130 788 421 Fragments arranged by size: 788 421 130 71 45 9 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 190 1464 Size: 190 1274 BtsI GCAGTG_nn' Cuts at: 0 705 898 1464 Size: 705 193 566 Fragments arranged by size: 705 566 193 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 237 272 1464 Size: 237 35 1192 Fragments arranged by size: 1192 237 35 DraIII CAC_nnn'GTG Cuts at: 0 720 782 1464 Size: 720 62 682 Fragments arranged by size: 720 682 62 EaeI y'GGCC_r Cuts at: 0 183 416 506 515 1205 1464 Size: 183 233 90 9 690 259 Fragments arranged by size: 690 259 233 183 90 9 EarI CTCTTCn'nnn_ Cuts at: 0 77 616 628 772 1027 1464 Size: 77 539 12 144 255 437 Fragments arranged by size: 539 437 255 144 77 12 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 94 1267 1464 Size: 94 1173 197 Fragments arranged by size: 1173 197 94 EcoICRI GAG'CTC Cuts at: 0 792 1053 1464 Size: 792 261 411 Fragments arranged by size: 792 411 261 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 228 334 394 554 856 991 1464 Size: 228 106 60 160 302 135 473 Fragments arranged by size: 473 302 228 160 135 106 60 EcoO109I rG'GnC_Cy Cuts at: 0 109 1446 1464 Size: 109 1337 18 Fragments arranged by size: 1337 109 18 HaeII r_GCGC'y Cuts at: 0 27 459 592 828 966 1464 Size: 27 432 133 236 138 498 Fragments arranged by size: 498 432 236 138 133 27 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 189 221 628 660 1464 Size: 189 32 407 32 804 Fragments arranged by size: 804 407 189 32 32 HincII GTy'rAC Cuts at: 0 642 1464 Size: 642 822 KasI G'GCGC_C Cuts at: 0 23 1464 Size: 23 1441 MscI TGG'CCA Cuts at: 0 508 517 1207 1464 Size: 508 9 690 257 Fragments arranged by size: 690 508 257 9 MslI CAynn'nnrTG Cuts at: 0 340 355 571 1138 1297 1464 Size: 340 15 216 567 159 167 Fragments arranged by size: 567 340 216 167 159 15 MspA1I CmG'CkG Cuts at: 0 11 103 127 182 538 1366 1453 1464 Size: 11 92 24 55 356 828 87 11 Fragments arranged by size: 828 356 92 87 55 24 11 11 NaeI GCC'GGC Cuts at: 0 171 1464 Size: 171 1293 NarI GG'CG_CC Cuts at: 0 24 1464 Size: 24 1440 NgoMIV G'CCGG_C Cuts at: 0 169 1464 Size: 169 1295 PmlI CAC'GTG Cuts at: 0 504 1464 Size: 504 960 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 841 873 1464 Size: 841 32 591 Fragments arranged by size: 841 591 32 PspOMI G'GGCC_C Cuts at: 0 108 1464 Size: 108 1356 PspXI vC'TCGA_Gb Cuts at: 0 48 1464 Size: 48 1416 PstI C_TGCA'G Cuts at: 0 215 684 1464 Size: 215 469 780 Fragments arranged by size: 780 469 215 PvuII CAG'CTG Cuts at: 0 1366 1464 Size: 1366 98 SacI G_AGCT'C Cuts at: 0 794 1055 1464 Size: 794 261 409 Fragments arranged by size: 794 409 261 SacII CC_GC'GG Cuts at: 0 12 128 1464 Size: 12 116 1336 Fragments arranged by size: 1336 116 12 SapI GCTCTTCn'nnn_ Cuts at: 0 628 1027 1464 Size: 628 399 437 Fragments arranged by size: 628 437 399 SbfI CC_TGCA'GG Cuts at: 0 215 1464 Size: 215 1249 SexAI A'CCwGG_T Cuts at: 0 1250 1464 Size: 1250 214 SfcI C'TryA_G Cuts at: 0 211 384 680 1464 Size: 211 173 296 784 Fragments arranged by size: 784 296 211 173 SfiI GGCCn_nnn'nGGCC Cuts at: 0 514 1464 Size: 514 950 SfoI GGC'GCC Cuts at: 0 25 1464 Size: 25 1439 SgrAI Cr'CCGG_yG Cuts at: 0 169 1464 Size: 169 1295 SmlI C'TyrA_G Cuts at: 0 48 291 616 847 1464 Size: 48 243 325 231 617 Fragments arranged by size: 617 325 243 231 48 StyI C'CwwG_G Cuts at: 0 668 1464 Size: 668 796 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 457 880 1464 Size: 457 423 584 Fragments arranged by size: 584 457 423 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 184 216 1464 Size: 184 32 1248 Fragments arranged by size: 1248 184 32 Tth111I GACn'n_nGTC Cuts at: 0 36 1464 Size: 36 1428 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1351 1464 Size: 1351 113 XhoI C'TCGA_G Cuts at: 0 48 1464 Size: 48 1416 XmnI GAAnn'nnTTC Cuts at: 0 928 1464 Size: 928 536 ZraI GAC'GTC Cuts at: 0 639 1464 Size: 639 825 Enzymes that do cut: AatII AcuI AfeI AleI AlwNI ApaI ApaLI AvaI BamHI BanI BanII BbeI BbvCI BglI BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BsrBI BsrDI BsrFI BssSI BstXI BstYI BtgI BtgZI BtsI CspCI DraIII EaeI EarI EciI EcoICRI Eco57MI EcoO109I HaeII Hin4I HincII KasI MscI MslI MspA1I NaeI NarI NgoMIV PmlI PpiI PspOMI PspXI PstI PvuII SacI SacII SapI SbfI SexAI SfcI SfiI SfoI SgrAI SmlI StyI TaqII TstI Tth111I XcmI XhoI XmnI ZraI Enzymes that do not cut: AarI AccI Acc65I AclI AflII AflIII AgeI AhdI AlfI AloI ApoI AscI AseI AsiSI AvrII BaeI BbsI BcgI BciVI BclI BfrBI BglII BmtI BsiWI BspHI BspMI BsrGI BssHII BstAPI BstBI BstEII BstZ17I Bsu36I ClaI DraI DrdI EagI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HindIII HpaI KpnI MfeI MluI MmeI NcoI NdeI NheI NotI NruI NsiI NspI PacI PasI PciI PflMI PfoI PmeI PpuMI PshAI PsiI PsrI PvuI RsrII SalI SanDI ScaI SgfI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI TatI XbaI XmaI