Restriction map for
pFN21AB5793
##################################
# NO CUT ENZYMES ( 89 enzymes) #
##################################
AatII AclI AflII AgeI AhdI
Alw44I Aor51HI ApaI ApaLI AscI
AseI AsnI AspEI AvaI AviII
AvrII BbeI BbrPI BbsI BcgI
BfrI BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsiWI BsoBI BspLU11I
BspMI BssSI Bst1107I BstBI BstEII
BstPI BstZ17I CelII CpoI DraIII
DrdI Eam1105I Eco47III EcoO65I FseI
FspI HaeII KasI MamI MfeI
MluI MunI NaeI NarI NgoMIV
NheI NruI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RsrII SacI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI Sse8387I SspI StuI SwaI
XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
Acc65I 2141 GGTACC G^GTACC
AccIII 2238 TCCGGA T^CCGGA
AflIII 1679 ACACGT A^C[GA][CT]GT
AocI 1317 CCTGAGG CC^T[ACGT]AGG
Asp700I 1918 GAACAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2141 GGTACC G^GTACC
AspI 271 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 977 GGATCC G^GATCC
BanI 2141 GGTACC G^G[CT][GA]CC
BanII 845 GGGCTC G[GA]GC[CT]^C
BbvCI 93 CCTCAGC CCTCAGC(-5/-2)
BclI 1283 TGATCA T^GATCA
BglI 280 GCCAAGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaI 1791 GGTCTC GGTCTC(1/5)
BseAI 2238 TCCGGA T^CCGGA
BsgI 1371 GTGCAG GTGCAG(16/14)
BsmBI 1201 GAGACG CGTCTC(1/5)
BsmI 1446 GAATGC GAATGC(1/-1)
Bsp1407I 1514 TGTACA T^GTACA
BspDI 2369 ATCGAT AT^CGAT
BspEI 2238 TCCGGA T^CCGGA
BspHI 2463 TCATGA T^CATGA
BsrBI 4 GAGCGG CCGCTC(-3/-3)
BsrDI 1464 CATTGC GCAATG(2/0)
BsrFI 104 ACCGGC [GA]^CCGG[CT]
BsrGI 1514 TGTACA T^GTACA
BssHII 233 GCGCGC G^CGCGC
BstAPI 72 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 1317 CCTGAGG CC^T[ACGT]AGG
Cfr10I 104 ACCGGC [GA]^CCGG[CT]
ClaI 2369 ATCGAT AT^CGAT
DraI 1810 TTTAAA TTT^AAA
Eco81I 1317 CCTGAGG CC^T[ACGT]AGG
EcoNI 93 CCTCAGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 1816 GATATC GAT^ATC
EcoT22I 1087 ATGCAT ATGCA^T
FbaI 1283 TGATCA T^GATCA
HindIII 882 AAGCTT A^AGCTT
HpaI 549 GTTAAC GTT^AAC
KpnI 2141 GGTACC GGTAC^C
MroI 2238 TCCGGA T^CCGGA
NcoI 45 CCATGG C^CATGG
NdeI 1854 CATATG CA^TATG
NotI 184 GCGGCCGC GC^GGCCGC
NsiI 1087 ATGCAT ATGCA^T
PflFI 271 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 1654 GACGAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 73 CAGCTG CAG^CTG
RcaI 2463 TCATGA T^CATGA
SalI 87 GTCGAC G^TCGAC
SphI 2391 GCATGC GCATG^C
SspBI 1514 TGTACA T^GTACA
Tth111I 271 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 1490 CCATCTCAGTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1918 GAACAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AccI 87 GTCGAC GT^[AC][GT]AC
1237 GTCTAC
AcyI 224 GGCGTC G[GA]^CG[CT]C
1234 GGCGTC
BciVI 963 GGATAC GTATCC(6/5)
334 GTATCC
BglII 474 AGATCT A^GATCT
630 AGATCT
BsaHI 224 GGCGTC G[GA]^CG[CT]C
1234 GGCGTC
BsaWI 2144 ACCGGA [AT]^CCGG[AT]
2238 TCCGGA
EarI 1837 GAAGAG CTCTTC(1/4)
2114 GAAGAG
EcoRI 675 GAATTC G^AATTC
2250 GAATTC
Hin1I 224 GGCGTC G[GA]^CG[CT]C
1234 GGCGTC
HincII 87 GTCGAC GT[CT]^[GA]AC
549 GTTAAC
HindII 87 GTCGAC GT[CT]^[GA]AC
549 GTTAAC
HphI 165 GGTGA GGTGA(8/7)
1685 TCACC
Ksp632I 1837 GAAGAG CTCTTC(1/4)
2114 GAAGAG
KspI 210 CCGCGG CCGC^GG
1230 CCGCGG
PflMI 1781 CCAGCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2088 CCACGACCTGG
PstI 70 CTGCAG CTGCA^G
2383 CTGCAG
SacII 210 CCGCGG CCGC^GG
1230 CCGCGG
Van91I 1781 CCAGCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2088 CCACGACCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 96 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI AspHI AvaII
BalI BbvI BcnI BfaI BmyI
BpmI BsaJI BseRI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI BstUI
BstXI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EagI EclXI Eco52I EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII ItaI MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PpuMI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII