(Circular) (Six-base) MAPSORT of: pFN21AB5793.seq Check: 7747 from: 1 to: 7198 pFN21AB5793 7198 bp With 182 enzymes: SgfI * August 16, 2010 14:53 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5008 278 Size: 53 83 186 936 3472 2468 Fragments arranged by size: 3472 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2093 3243 4541 1937 Size: 156 1150 1298 4594 Fragments arranged by size: 4594 1298 1150 156 Acc65I G'GTAC_C Cuts at: 4146 4519 4146 Size: 373 6825 AclI AA'CG_TT Cuts at: 1563 5325 5698 1563 Size: 3762 373 3063 Fragments arranged by size: 3762 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2581 2856 3708 3906 4106 5261 6645 6913 Size: 949 275 852 198 200 1155 1384 268 Cuts at: 6913 1632 Size: 1917 Fragments arranged by size: 1917 1384 1155 949 852 275 268 200 198 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7001 AflIII A'CryG_T Cuts at: 1203 3684 6097 7074 1203 Size: 2481 2413 977 1327 Fragments arranged by size: 2481 2413 1327 977 AgeI A'CCGG_T Cuts at: 6029 6029 Size: 7198 AhdI GACnn_n'nnGTC Cuts at: 5927 5927 Size: 7198 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7198 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4413 1184 Size: 3229 3969 AlwNI CAG_nnn'CTG Cuts at: 2083 3418 3731 3884 3931 4941 6513 2083 Size: 1335 313 153 47 1010 1572 2768 Fragments arranged by size: 2768 1572 1335 1010 313 153 47 ApaLI G'TGCA_C Cuts at: 5255 6411 5255 Size: 1156 6042 ApoI r'AATT_y Cuts at: 1493 2510 2620 2680 2734 4155 4255 4267 4507 Size: 1017 110 60 54 1421 100 12 240 Cuts at: 4507 4733 4885 6994 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 1421 1017 240 226 152 110 100 60 54 12 AseI AT'TA_AT Cuts at: 160 1032 5752 160 Size: 872 4720 1606 Fragments arranged by size: 4720 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7198 AvaI C'yCGr_G Cuts at: 1870 1943 4523 1870 Size: 73 2580 4545 Fragments arranged by size: 4545 2580 73 BamHI G'GATC_C Cuts at: 2982 4528 4895 2982 Size: 1546 367 5285 Fragments arranged by size: 5285 1546 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4146 4519 5974 618 Size: 325 217 627 2359 373 1455 1842 Fragments arranged by size: 2359 1842 1455 627 373 325 217 BanII G_rGCy'C Cuts at: 1391 2854 4517 1391 Size: 1463 1663 4072 Fragments arranged by size: 4072 1663 1463 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 6227 BbvCI CC'TCA_GC Cuts at: 2099 2099 Size: 7198 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5389 5423 1903 Size: 34 3452 34 3678 Fragments arranged by size: 3678 3452 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2350 2962 5090 6300 2350 Size: 612 2128 1210 3248 Fragments arranged by size: 3248 2128 1210 612 BclI T'GATC_A Cuts at: 1548 3288 1548 Size: 1740 5458 BfrBI ATG'CAT Cuts at: 3094 3094 Size: 7198 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2291 5809 136 Size: 107 122 71 631 605 619 3518 1525 Fragments arranged by size: 3518 1525 631 619 605 122 107 71 BglII A'GATC_T Cuts at: 2479 2635 7193 2479 Size: 156 4558 2484 Fragments arranged by size: 4558 2484 156 BlpI GC'TnA_GC Cuts at: 4615 4615 Size: 7198 Bme1580I G_kGCm'C Cuts at: 1790 5259 6415 1790 Size: 3469 1156 2573 Fragments arranged by size: 3469 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7198 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2376 3126 4929 Size: 416 53 83 399 24 959 750 1803 Cuts at: 4929 5887 442 Size: 958 1753 Fragments arranged by size: 1803 1753 959 958 750 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7198 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2592 3129 3615 3753 4034 4121 5858 1239 Size: 1353 537 486 138 281 87 1737 2579 Fragments arranged by size: 2579 1737 1353 537 486 281 138 87 Bpu10I CC'TnA_GC Cuts at: 2099 2099 Size: 7198 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5323 6188 6486 5323 Size: 865 298 6035 Fragments arranged by size: 6035 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3802 5861 882 Size: 617 2303 2059 2219 Fragments arranged by size: 2303 2219 2059 617 BsaAI yAC'GTr Cuts at: 493 1204 4989 493 Size: 711 3785 2702 Fragments arranged by size: 3785 2702 711 BsaBI GATnn'nnATC Cuts at: 7192 7192 Size: 7198 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2230 3240 5005 5387 Size: 53 83 186 936 697 1010 1765 382 Cuts at: 5387 6022 275 Size: 635 1451 Fragments arranged by size: 1765 1451 1010 936 697 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4149 4243 5631 6029 6303 6450 6861 4149 Size: 94 1388 398 274 147 411 4486 Fragments arranged by size: 4486 1388 411 398 274 147 94 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3212 3242 3392 3422 4427 Size: 30 165 30 1809 30 150 30 1005 Cuts at: 4427 4457 1178 Size: 30 3919 Fragments arranged by size: 3919 1809 1005 165 150 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2054 2057 2060 2270 1186 Size: 868 3 3 210 6114 Fragments arranged by size: 6114 868 210 3 3 BseYI C'CCAG_C Cuts at: 2175 3024 6401 6897 2175 Size: 849 3377 496 2476 Fragments arranged by size: 3377 2476 849 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3397 1163 Size: 573 1661 4964 Fragments arranged by size: 4964 1661 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2193 2224 2266 4648 5409 6437 Size: 275 186 192 31 42 2382 761 1028 Cuts at: 6437 1540 Size: 2301 Fragments arranged by size: 2382 2301 1028 761 275 192 186 42 31 BsiHKAI G_wGCw'C Cuts at: 3261 3621 4332 5259 5344 6415 3261 Size: 360 711 927 85 1071 4044 Fragments arranged by size: 4044 1071 927 711 360 85 BsmI GAATG_Cn' Cuts at: 3457 4709 4802 3457 Size: 1252 93 5853 Fragments arranged by size: 5853 1252 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3200 3200 Size: 7198 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2854 3261 3621 4332 4517 5259 5344 Size: 399 1064 407 360 711 185 742 85 Cuts at: 5344 6415 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 1064 742 711 407 399 360 185 85 BspEI T'CCGG_A Cuts at: 4243 6861 4243 Size: 2618 4580 BspHI T'CATG_A Cuts at: 4468 5087 6817 4468 Size: 619 1730 4849 Fragments arranged by size: 4849 1730 619 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4553 844 Size: 3709 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2011 5085 1394 Size: 429 188 3074 3507 Fragments arranged by size: 3507 3074 429 188 BsrDI GCAATG_nn' Cuts at: 65 1248 3468 5693 5867 65 Size: 1183 2220 2225 174 1396 Fragments arranged by size: 2225 2220 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2109 5842 6029 1700 Size: 110 299 3733 187 2869 Fragments arranged by size: 3733 2869 299 187 110 BsrGI T'GTAC_A Cuts at: 96 3519 96 Size: 3423 3775 BssHII G'CGCG_C Cuts at: 1925 2238 1925 Size: 313 6885 BssSI C'ACGA_G Cuts at: 5258 6270 5258 Size: 1012 6186 BstAPI GCAn_nnn'nTGC Cuts at: 794 2083 794 Size: 1289 5909 BstBI TT'CG_AA Cuts at: 6002 6002 Size: 7198 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7198 BstXI CCAn_nnnn'nTGG Cuts at: 3172 3865 4069 4414 3172 Size: 693 204 345 5956 Fragments arranged by size: 5956 693 345 204 BstYI r'GATC_y Cuts at: 1972 2479 2635 2982 4528 4895 5280 5297 6749 Size: 507 156 347 1546 367 385 17 1452 Cuts at: 6749 6835 6847 7193 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1546 1452 507 385 367 347 346 156 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3323 3323 Size: 7198 BtgI C'CryG_G Cuts at: 513 1065 2004 2050 2215 3036 3235 513 Size: 552 939 46 165 821 199 4476 Fragments arranged by size: 4476 939 821 552 199 165 46 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6459 Fragments arranged by size: 6459 683 56 BtsI GCAGTG_nn' Cuts at: 4714 5508 5528 4714 Size: 794 20 6384 Fragments arranged by size: 6384 794 20 ClaI AT'CG_AT Cuts at: 1554 4375 1554 Size: 2821 4377 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7163 DraI TTT'AAA Cuts at: 3817 4502 3817 Size: 685 6513 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6205 809 Size: 507 4889 1802 Fragments arranged by size: 4889 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2190 2221 2263 Size: 54 1425 50 275 18 360 31 42 Cuts at: 2263 2292 2778 3609 4287 4645 5534 7163 8 Size: 29 486 831 678 358 889 1629 43 Fragments arranged by size: 1629 1425 889 831 678 486 360 358 275 54 50 43 42 31 29 18 EagI C'GGCC_G Cuts at: 1537 1812 2190 2221 2263 4645 1537 Size: 275 378 31 42 2382 4090 Fragments arranged by size: 4090 2382 378 275 42 31 EarI CTCTTCn'nnn_ Cuts at: 1353 3837 4114 5128 1353 Size: 2484 277 1014 3423 Fragments arranged by size: 3423 2484 1014 277 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3216 5785 6159 6305 387 Size: 1064 138 1627 2569 374 146 1280 Fragments arranged by size: 2569 1627 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2581 2592 2856 3129 3615 3708 3753 Size: 393 949 11 264 273 486 93 45 Cuts at: 3753 3906 4034 4106 4121 5261 5858 6645 6913 Size: 153 128 72 15 1140 597 787 268 Cuts at: 6913 1239 Size: 1524 Fragments arranged by size: 1524 1140 949 787 597 486 393 273 268 264 153 128 93 72 45 15 11 EcoNI CCTnn'n_nnAGG Cuts at: 2102 2102 Size: 7198 EcoO109I rG'GnC_Cy Cuts at: 3566 3697 4486 3566 Size: 131 789 6278 Fragments arranged by size: 6278 789 131 EcoRI G'AATT_C Cuts at: 2680 4255 4507 4885 2680 Size: 1575 252 378 4993 Fragments arranged by size: 4993 1575 378 252 EcoRV GAT'ATC Cuts at: 3823 3823 Size: 7198 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2861 3842 2861 Size: 981 6217 FspI TGC'GCA Cuts at: 5704 5704 Size: 7198 HaeII r_GCGC'y Cuts at: 1678 6345 1678 Size: 4667 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2021 2053 2577 2609 3141 Size: 32 135 32 449 32 524 32 532 Cuts at: 3141 3173 3482 3514 4427 4459 5839 5871 5913 Size: 32 309 32 913 32 1380 32 42 Cuts at: 5913 5945 1373 Size: 32 2626 Fragments arranged by size: 2626 1380 913 532 524 449 309 135 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2094 2556 4542 4788 669 Size: 988 281 156 462 1986 246 3079 Fragments arranged by size: 3079 1986 988 462 281 246 156 HindIII A'AGCT_T Cuts at: 748 2887 748 Size: 2139 5059 HpaI GTT'AAC Cuts at: 2556 4788 2556 Size: 2232 4966 KpnI G_GTAC'C Cuts at: 4150 4523 4150 Size: 373 6825 MfeI C'AATT_G Cuts at: 4797 4797 Size: 7198 MluI A'CGCG_T Cuts at: 7074 7074 Size: 7198 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2335 6312 6496 1305 Size: 1030 3977 184 2007 Fragments arranged by size: 3977 2007 1030 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2294 2780 3611 4289 10 Size: 54 1425 343 462 486 831 678 2919 Fragments arranged by size: 2919 1425 831 678 486 462 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2168 2310 2660 3099 3390 3500 3605 Size: 648 1002 142 350 439 291 110 105 Cuts at: 3605 3952 5156 5515 5674 518 Size: 347 1204 359 159 2042 Fragments arranged by size: 2042 1204 1002 648 439 359 350 347 291 159 142 110 105 MspA1I CmG'CkG Cuts at: 1606 2080 2135 2143 2217 3237 4614 5291 6439 Size: 474 55 8 74 1020 1377 677 1148 Cuts at: 6439 6684 1606 Size: 245 2120 Fragments arranged by size: 2120 1377 1148 1020 677 474 245 74 55 8 NcoI C'CATG_G Cuts at: 513 1065 2004 2050 513 Size: 552 939 46 5661 Fragments arranged by size: 5661 939 552 46 NdeI CA'TA_TG Cuts at: 387 3860 387 Size: 3473 3725 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7198 NotI GC'GGCC_GC Cuts at: 2190 4645 2190 Size: 2455 4743 NsiI A_TGCA'T Cuts at: 3096 3096 Size: 7198 NspI r_CATG'y Cuts at: 1132 1229 2315 3098 4400 4556 6101 1132 Size: 97 1086 783 1302 156 1545 2229 Fragments arranged by size: 2229 1545 1302 1086 783 156 97 PasI CC'CwG_GG Cuts at: 1348 2016 1348 Size: 668 6530 PciI A'CATG_T Cuts at: 6097 6097 Size: 7198 PflMI CCAn_nnn'nTGG Cuts at: 1108 3792 4099 1108 Size: 2684 307 4207 Fragments arranged by size: 4207 2684 307 PfoI T'CCnGG_A Cuts at: 2271 3545 3585 2271 Size: 1274 40 5884 Fragments arranged by size: 5884 1274 40 PmeI GTTT'AAAC Cuts at: 4502 4502 Size: 7198 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5267 5299 6780 6812 5267 Size: 32 1481 32 5653 Fragments arranged by size: 5653 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3566 3697 4486 3566 Size: 131 789 6278 Fragments arranged by size: 6278 789 131 PshAI GACnn'nnGTC Cuts at: 1613 3663 1613 Size: 2050 5148 PsiI TTA'TAA Cuts at: 4768 4768 Size: 7198 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 4176 4208 4176 Size: 32 7166 PstI C_TGCA'G Cuts at: 830 2079 4392 4550 830 Size: 1249 2313 158 3478 Fragments arranged by size: 3478 2313 1249 158 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7198 PvuII CAG'CTG Cuts at: 2080 2080 Size: 7198 SacII CC_GC'GG Cuts at: 2218 3238 2218 Size: 1020 6178 SalI G'TCGA_C Cuts at: 1936 2092 4540 1936 Size: 156 2448 4594 Fragments arranged by size: 4594 2448 156 SanDI GG'GwC_CC Cuts at: 3697 3697 Size: 7198 SbfI CC_TGCA'GG Cuts at: 4550 4550 Size: 7198 ScaI AGT'ACT Cuts at: 5446 7158 5446 Size: 1712 5486 SfcI C'TryA_G Cuts at: 826 1045 2075 3354 3400 4388 4546 5681 6362 Size: 219 1030 1279 46 988 158 1135 681 Cuts at: 6362 6553 826 Size: 191 1471 Fragments arranged by size: 1471 1279 1135 1030 988 681 219 191 158 46 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7198 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7198 SmaI CCC'GGG Cuts at: 1872 4525 1872 Size: 2653 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 5302 6203 6465 820 Size: 197 926 3359 901 262 1553 Fragments arranged by size: 3359 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4989 493 Size: 4496 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7198 SphI G_CATG'C Cuts at: 1132 4400 4556 1132 Size: 3268 156 3774 Fragments arranged by size: 3774 3268 156 SspI AAT'ATT Cuts at: 5 52 5122 5 Size: 47 5070 2081 Fragments arranged by size: 5070 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7198 StyI C'CwwG_G Cuts at: 513 1065 2004 2050 2845 3301 4637 513 Size: 552 939 46 795 456 1336 3074 Fragments arranged by size: 3074 1336 939 795 552 456 46 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2713 3192 5225 5242 5395 5580 6025 Size: 1255 1133 479 2033 17 153 185 445 Cuts at: 6025 325 Size: 1498 Fragments arranged by size: 2033 1498 1255 1133 479 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3519 5444 7156 Size: 274 80 33 51 1443 1542 1925 1712 Cuts at: 7156 96 Size: 138 Fragments arranged by size: 1925 1712 1542 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2243 2275 2535 2567 7158 7190 2243 Size: 32 260 32 4591 32 2251 Fragments arranged by size: 4591 2251 260 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2279 1316 Size: 963 6235 XbaI T'CTAG_A Cuts at: 4534 4534 Size: 7198 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3502 3502 Size: 7198 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7198 XmaI C'CCGG_G Cuts at: 1870 4523 1870 Size: 2653 4545 XmnI GAAnn'nnTTC Cuts at: 3927 5327 3927 Size: 1400 5798 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5006 276 Size: 53 83 186 936 3472 2468 Fragments arranged by size: 3472 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SacII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BplI BsiWI BstZ17I DraIII EcoICRI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI RsrII SacI SapI SexAI SfiI SfoI SrfI SwaI