################################## # NO CUT ENZYMES ( 134 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AcyI AflII AflIII AhdI Alw44I ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AviII AvrII BalI BamHI BbeI BbrPI BcgI BclI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BseAI BsiEI BsiWI BsmBI BspDI BspEI BspHI BspLU11I BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstYI BstZ17I CelII ClaI CpoI DraI DraIII DrdI EaeI EagI Eam1105I EarI EclXI Eco52I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HgaI Hin1I HindIII HpaI KasI KpnI Ksp632I KspI MamI MfeI MflI MluI MluNI MroI MscI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PmaCI PmeI PmlI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI SwaI XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AccII 779 CGCG CG^CG AfaI 1070 GTAC GT^AC AgeI 461 ACCGGT A^CCGGT Aor51HI 1037 AGCGCT AGC^GCT AspHI 106 GAGCAC G[AT]GC[AT]^C AspI 212 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC BanI 906 GGTGCC G^G[CT][GA]CC BbsI 1128 GAAGAC GAAGAC(2/6) BbvCI 96 GCTGAGG CCTCAGC(-5/-2) BciVI 1089 GGATAC GTATCC(6/5) BglI 1189 GCCCGTCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpuAI 1128 GAAGAC GAAGAC(2/6) BsaWI 461 ACCGGT [AT]^CCGG[AT] BsiHKAI 106 GAGCAC G[AT]GC[AT]^C Bsp1407I 1069 TGTACA T^GTACA BsrBI 954 CCGCTC CCGCTC(-3/-3) BsrDI 718 GCAATG GCAATG(2/0) BsrGI 1069 TGTACA T^GTACA BstUI 779 CGCG CG^CG DraII 306 GGGTCCC [GA]G^G[ACGT]CC[CT] DsaI 167 CCATGG C^C[GA][CT]GG Eco47III 1037 AGCGCT AGC^GCT EcoO109I 306 GGGTCCC [GA]G^G[ACGT]CC[CT] HincII 1486 GTCAAC GT[CT]^[GA]AC HindII 1486 GTCAAC GT[CT]^[GA]AC MseI 1396 TTAA T^TAA MvnI 779 CGCG CG^CG NcoI 167 CCATGG C^CATGG NspI 558 GCATGT [GA]CATG^[CT] PflFI 212 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 483 CCACCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 461 ACCGGT A^CCGGT PpuMI 306 GGGTCCC [GA]G^G[AT]CC[CT] PshAI 1237 GACATCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RsaI 1070 GTAC GT^AC SfcI 80 CTACAG C^T[GA][CT]AG SmlI 883 CTTGAG C^T[CT][GA]AG SspBI 1069 TGTACA T^GTACA TaqI 1245 TCGA T^CGA TfiI 728 GATTC G^A[AT]TC Tru9I 1396 TTAA T^TAA Tth111I 212 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 1245 TCGA T^CGA Van91I 483 CCACCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AlwNI 419 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG 977 CAGCTACTG AocI 319 CCTCAGG CC^T[ACGT]AGG 942 CCTGAGG AvaI 876 CTCGGG C^[CT]CG[GA]G 1172 CTCGGG BfaI 688 CTAG C^TAG 1525 CTAG BsaI 622 GAGACC GGTCTC(1/5) 1442 GAGACC BsmI 386 GAATGC GAATGC(1/-1) 435 GAATGC BsoBI 876 CTCGGG C^[CT]CG[GA]G 1172 CTCGGG BsrFI 461 ACCGGT [GA]^CCGG[CT] 485 ACCGGC BsrI 767 ACTGG ACTGG(1/-1) 1576 ACTGG BstXI 1256 CCAACCTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1269 CCACCTCTGTGG Bsu36I 319 CCTCAGG CC^T[ACGT]AGG 942 CCTGAGG CfoI 948 GCGC GCG^C 1038 GCGC Cfr10I 461 ACCGGT [GA]^CCGG[CT] 485 ACCGGC Eco81I 319 CCTCAGG CC^T[ACGT]AGG 942 CCTGAGG FauI 653 CCCGC CCCGC(4/6) 1340 CCCGC HaeII 947 GGCGCT [GA]GCGC^[CT] 1037 AGCGCT HhaI 948 GCGC GCG^C 1038 GCGC HinP1I 948 GCGC G^CGC 1038 GCGC MaeI 688 CTAG C^TAG 1525 CTAG MaeII 1428 ACGT A^CGT 1517 ACGT MboII 1128 GAAGA GAAGA(8/7) 1615 GAAGA MslI 364 CACCCCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1270 CACCTCTGTG PvuII 94 CAGCTG CAG^CTG 1018 CAGCTG StuI 1079 AGGCCT AGG^CCT 1370 AGGCCT XcmI 14 CCAGAGCAGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 483 CCACCGGCTGGATGG ######################################### # 3 OR MORE CUT ENZYMES ( 55 enzymes) # ######################################### AciI AluI AlwI AvaII BanII BbvI BcnI BmyI BpmI BsaJI BseRI BsgI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI MaeIII MboI MnlI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI Sau3AI Sau96I ScrFI StyI TseI Tsp45I Tsp509I TspRI