(Circular) (Six-base) MAPSORT of: pFN21AE4512.seq Check: 1316 from: 1 to: 6325 pFN21AE4512 6325 bp With 182 enzymes: SgfI * March 28, 2012 14:06 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4135 278 Size: 53 83 186 936 2599 2468 Fragments arranged by size: 2599 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3668 1937 Size: 1731 4594 Acc65I G'GTAC_C Cuts at: 3646 3646 Size: 6325 AclI AA'CG_TT Cuts at: 1563 4452 4825 1563 Size: 2889 373 3063 Fragments arranged by size: 3063 2889 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4388 5772 6040 1632 Size: 2756 1384 268 1917 Fragments arranged by size: 2756 1917 1384 268 AfeI AGC'GCT Cuts at: 3044 3044 Size: 6325 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6128 AflIII A'CryG_T Cuts at: 1203 5224 6201 1203 Size: 4021 977 1327 Fragments arranged by size: 4021 1327 977 AgeI A'CCGG_T Cuts at: 2466 5156 2466 Size: 2690 3635 AhdI GACnn_n'nnGTC Cuts at: 5054 5054 Size: 6325 AleI CACnn'nnGTG Cuts at: 1166 3279 1166 Size: 2113 4212 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6325 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2786 2818 3020 3052 2786 Size: 32 202 32 6059 Fragments arranged by size: 6059 202 32 32 AlwNI CAG_nnn'CTG Cuts at: 2429 2987 4068 5640 2429 Size: 558 1081 1572 3114 Fragments arranged by size: 3114 1572 1081 558 ApaLI G'TGCA_C Cuts at: 4382 5538 4382 Size: 1156 5169 ApoI r'AATT_y Cuts at: 1493 3634 3860 4012 6121 1493 Size: 2141 226 152 2109 1697 Fragments arranged by size: 2141 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4879 160 Size: 872 3847 1606 Fragments arranged by size: 3847 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6325 AvaI C'yCGr_G Cuts at: 1870 1943 2881 3177 3650 1870 Size: 73 938 296 473 4545 Fragments arranged by size: 4545 938 473 296 73 BamHI G'GATC_C Cuts at: 3655 4022 3655 Size: 367 5958 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2911 3646 5101 618 Size: 325 217 627 1124 735 1455 1842 Fragments arranged by size: 1842 1455 1124 735 627 325 217 BanII G_rGCy'C Cuts at: 1391 3205 3578 3615 3644 1391 Size: 1814 373 37 29 4072 Fragments arranged by size: 4072 1814 373 37 29 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3140 928 Size: 971 1241 4113 Fragments arranged by size: 4113 1241 971 BbvCI CC'TCA_GC Cuts at: 2102 2102 Size: 6325 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4516 4550 1903 Size: 34 2579 34 3678 Fragments arranged by size: 3678 2579 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3088 4217 5427 3088 Size: 1129 1210 3986 Fragments arranged by size: 3986 1210 1129 BclI T'GATC_A Cuts at: 1548 1548 Size: 6325 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3200 4936 136 Size: 107 122 71 631 605 1528 1736 1525 Fragments arranged by size: 1736 1528 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6320 6320 Size: 6325 BlpI GC'TnA_GC Cuts at: 3742 3742 Size: 6325 Bme1580I G_kGCm'C Cuts at: 1790 2916 4386 5542 1790 Size: 1126 1470 1156 2573 Fragments arranged by size: 2573 1470 1156 1126 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6325 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4056 5014 442 Size: 416 53 83 399 24 2639 958 1753 Fragments arranged by size: 2639 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6325 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2374 3592 2374 Size: 1218 5107 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2348 2508 2663 3047 3558 3603 4985 1239 Size: 1109 160 155 384 511 45 1382 2579 Fragments arranged by size: 2579 1382 1109 511 384 160 155 45 Bpu10I CC'TnA_GC Cuts at: 2102 2102 Size: 6325 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2909 4450 5315 5613 2909 Size: 1541 865 298 3621 Fragments arranged by size: 3621 1541 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2621 3441 4988 882 Size: 617 1122 820 1547 2219 Fragments arranged by size: 2219 1547 1122 820 617 BsaAI yAC'GTr Cuts at: 493 1204 4116 493 Size: 711 2912 2702 Fragments arranged by size: 2912 2702 711 BsaBI GATnn'nnATC Cuts at: 6319 6319 Size: 6325 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4132 4514 5149 275 Size: 53 83 186 936 2599 382 635 1451 Fragments arranged by size: 2599 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2466 4758 5156 5430 5577 5988 2466 Size: 2292 398 274 147 411 2803 Fragments arranged by size: 2803 2292 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2171 2201 2786 2816 3020 Size: 30 165 30 768 30 585 30 204 Cuts at: 3020 3050 1178 Size: 30 4453 Fragments arranged by size: 4453 768 585 204 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2179 3404 3407 1186 Size: 993 1225 3 4104 Fragments arranged by size: 4104 1225 993 3 BseYI C'CCAG_C Cuts at: 2448 2481 2829 2858 3167 5528 6024 2448 Size: 33 348 29 309 2361 496 2749 Fragments arranged by size: 2749 2361 496 348 309 33 29 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2249 2764 3405 1163 Size: 573 513 515 641 4083 Fragments arranged by size: 4083 641 573 515 513 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3775 4536 5564 1540 Size: 275 186 1774 761 1028 2301 Fragments arranged by size: 2301 1774 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2115 4386 4471 5542 2115 Size: 2271 85 1071 2898 Fragments arranged by size: 2898 2271 1071 85 BsmI GAATG_Cn' Cuts at: 2397 2446 3836 3929 2397 Size: 49 1390 93 4793 Fragments arranged by size: 4793 1390 93 49 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2115 2916 3205 3578 3615 3644 4386 Size: 399 325 801 289 373 37 29 742 Cuts at: 4386 4471 5542 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 801 742 399 373 325 289 85 37 29 BspEI T'CCGG_A Cuts at: 5988 5988 Size: 6325 BspHI T'CATG_A Cuts at: 4214 5944 4214 Size: 1730 4595 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2290 2985 3366 3680 844 Size: 1446 695 381 314 3489 Fragments arranged by size: 3489 1446 695 381 314 BsrBI CCG'CTC Cuts at: 1394 1823 2961 4212 1394 Size: 429 1138 1251 3507 Fragments arranged by size: 3507 1251 1138 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2730 4820 4994 65 Size: 1183 1482 2090 174 1396 Fragments arranged by size: 2090 1482 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2466 2490 4969 5156 1700 Size: 110 656 24 2479 187 2869 Fragments arranged by size: 2869 2479 656 187 110 24 BsrGI T'GTAC_A Cuts at: 96 3074 96 Size: 2978 3347 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6325 BssSI C'ACGA_G Cuts at: 4385 5397 4385 Size: 1012 5313 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6325 BstBI TT'CG_AA Cuts at: 5129 5129 Size: 6325 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6325 BstXI CCAn_nnnn'nTGG Cuts at: 3268 3281 3268 Size: 13 6312 BstYI r'GATC_y Cuts at: 1972 3655 4022 4407 4424 5876 5962 5974 6320 Size: 1683 367 385 17 1452 86 12 346 Cuts at: 6320 1972 Size: 1977 Fragments arranged by size: 1977 1683 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2325 2948 2325 Size: 623 5702 BtgI C'CryG_G Cuts at: 513 1065 2004 2172 513 Size: 552 939 168 4666 Fragments arranged by size: 4666 939 552 168 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5586 Fragments arranged by size: 5586 683 56 BtsI GCAGTG_nn' Cuts at: 2452 2668 3841 4635 4655 2452 Size: 216 1173 794 20 4122 Fragments arranged by size: 4122 1173 794 216 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6325 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3134 3169 529 Size: 35 2570 35 3685 Fragments arranged by size: 3685 2570 35 35 DraI TTT'AAA Cuts at: 3629 3629 Size: 6325 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5332 809 Size: 507 4016 1802 Fragments arranged by size: 4016 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3772 4661 6290 Size: 54 1425 50 275 18 1942 889 1629 Cuts at: 6290 8 Size: 43 Fragments arranged by size: 1942 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3772 1537 Size: 275 1960 4090 Fragments arranged by size: 4090 1960 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4255 1353 Size: 2902 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4912 5286 5432 387 Size: 1064 138 3323 374 146 1280 Fragments arranged by size: 3323 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2348 2508 2663 3047 3558 3603 4388 Size: 393 716 160 155 384 511 45 785 Cuts at: 4388 4985 5772 6040 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 787 785 716 597 511 393 384 268 160 155 45 EcoO109I rG'GnC_Cy Cuts at: 2312 2312 Size: 6325 EcoRI G'AATT_C Cuts at: 3634 4012 3634 Size: 378 5947 FspI TGC'GCA Cuts at: 4831 4831 Size: 6325 HaeII r_GCGC'y Cuts at: 1678 2956 3046 5472 1678 Size: 1278 90 2426 2531 Fragments arranged by size: 2531 2426 1278 90 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2864 2896 3423 3455 4966 Size: 32 135 32 1292 32 527 32 1511 Cuts at: 4966 4998 5040 5072 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1511 1292 527 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3493 3669 3915 669 Size: 988 281 1555 176 246 3079 Fragments arranged by size: 3079 1555 988 281 246 176 HindIII A'AGCT_T Cuts at: 748 748 Size: 6325 HpaI GTT'AAC Cuts at: 3915 3915 Size: 6325 KpnI G_GTAC'C Cuts at: 3650 3650 Size: 6325 MfeI C'AATT_G Cuts at: 3924 3924 Size: 6325 MluI A'CGCG_T Cuts at: 6201 6201 Size: 6325 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2287 5439 5623 1305 Size: 982 3152 184 2007 Fragments arranged by size: 3152 2007 982 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4503 Fragments arranged by size: 4503 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2373 3279 4283 4642 4801 518 Size: 648 1207 906 1004 359 159 2042 Fragments arranged by size: 2042 1207 1004 906 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2101 2661 3025 3348 3741 4418 5566 5811 Size: 495 560 364 323 393 677 1148 245 Cuts at: 5811 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 560 495 393 364 323 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2172 513 Size: 552 939 168 4666 Fragments arranged by size: 4666 939 552 168 NdeI CA'TA_TG Cuts at: 387 387 Size: 6325 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6325 NotI GC'GGCC_GC Cuts at: 3772 3772 Size: 6325 NspI r_CATG'y Cuts at: 1132 1229 2567 3683 5228 1132 Size: 97 1338 1116 1545 2229 Fragments arranged by size: 2229 1545 1338 1116 97 PasI CC'CwG_GG Cuts at: 1348 2510 1348 Size: 1162 5163 PciI A'CATG_T Cuts at: 5224 5224 Size: 6325 PflMI CCAn_nnn'nTGG Cuts at: 1108 2494 1108 Size: 1386 4939 PfoI T'CCnGG_A Cuts at: 2435 2640 2814 2435 Size: 205 174 5946 Fragments arranged by size: 5946 205 174 PmeI GTTT'AAAC Cuts at: 3629 3629 Size: 6325 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2732 2764 2786 2818 3020 3052 4394 4426 5907 Size: 32 22 32 202 32 1342 32 1481 Cuts at: 5907 5939 2732 Size: 32 3118 Fragments arranged by size: 3118 1481 1342 202 32 32 32 32 32 22 PpuMI rG'GwC_Cy Cuts at: 2312 2312 Size: 6325 PshAI GACnn'nnGTC Cuts at: 1613 3246 1613 Size: 1633 4692 PsiI TTA'TAA Cuts at: 3895 3895 Size: 6325 PstI C_TGCA'G Cuts at: 830 3677 830 Size: 2847 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6325 PvuII CAG'CTG Cuts at: 2101 3025 2101 Size: 924 5401 SalI G'TCGA_C Cuts at: 1936 3667 1936 Size: 1731 4594 SanDI GG'GwC_CC Cuts at: 2312 2312 Size: 6325 SbfI CC_TGCA'GG Cuts at: 3677 3677 Size: 6325 ScaI AGT'ACT Cuts at: 4573 6285 4573 Size: 1712 4613 SfcI C'TryA_G Cuts at: 826 1045 2085 3673 4808 5489 5680 826 Size: 219 1040 1588 1135 681 191 1471 Fragments arranged by size: 1588 1471 1135 1040 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6325 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6325 SmaI CCC'GGG Cuts at: 1872 3652 1872 Size: 1780 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2888 4429 5330 5592 820 Size: 197 926 945 1541 901 262 1553 Fragments arranged by size: 1553 1541 945 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4116 493 Size: 3623 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6325 SphI G_CATG'C Cuts at: 1132 3683 1132 Size: 2551 3774 SspI AAT'ATT Cuts at: 5 52 4249 5 Size: 47 4197 2081 Fragments arranged by size: 4197 2081 47 StuI AGG'CCT Cuts at: 1517 3086 3377 1517 Size: 1569 291 4465 Fragments arranged by size: 4465 1569 291 StyI C'CwwG_G Cuts at: 513 1065 2004 2028 2172 2473 3555 3615 3764 Size: 552 939 24 144 301 1082 60 149 Cuts at: 3764 513 Size: 3074 Fragments arranged by size: 3074 1082 939 552 301 149 144 60 24 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2743 4352 4369 4522 4707 5152 325 Size: 1255 1163 1609 17 153 185 445 1498 Fragments arranged by size: 1609 1498 1255 1163 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3074 4571 6283 Size: 274 80 33 51 1443 1097 1497 1712 Cuts at: 6283 96 Size: 138 Fragments arranged by size: 1712 1497 1443 1097 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2171 2203 6285 6317 2171 Size: 32 4082 32 2179 Fragments arranged by size: 4082 2179 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2220 1316 Size: 904 5421 XbaI T'CTAG_A Cuts at: 3661 3661 Size: 6325 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2026 2495 2026 Size: 469 5856 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6325 XmaI C'CCGG_G Cuts at: 1870 3650 1870 Size: 1780 4545 XmnI GAAnn'nnTTC Cuts at: 4454 4454 Size: 6325 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4133 276 Size: 53 83 186 936 2599 2468 Fragments arranged by size: 2599 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI