(Linear) (Six-base) MAPSORT of: KIEE4512.seq Check: 1224 from: 1 to: 1620 KIEE4512.orf With 182 enzymes: SgfI * March 22, 2012 18:14 .. AfeI AGC'GCT Cuts at: 0 1039 1620 Size: 1039 581 AgeI A'CCGG_T Cuts at: 0 461 1620 Size: 461 1159 AleI CACnn'nnGTG Cuts at: 0 1274 1620 Size: 1274 346 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 781 813 1015 1047 1620 Size: 781 32 202 32 573 Fragments arranged by size: 781 573 202 32 32 AlwNI CAG_nnn'CTG Cuts at: 0 424 982 1620 Size: 424 558 638 Fragments arranged by size: 638 558 424 AvaI C'yCGr_G Cuts at: 0 876 1172 1620 Size: 876 296 448 Fragments arranged by size: 876 448 296 BanI G'GyrC_C Cuts at: 0 906 1620 Size: 906 714 BanII G_rGCy'C Cuts at: 0 1200 1573 1610 1620 Size: 1200 373 37 10 Fragments arranged by size: 1200 373 37 10 BbsI GAAGACnn'nnnn_ Cuts at: 0 1135 1620 Size: 1135 485 BbvCI CC'TCA_GC Cuts at: 0 97 1620 Size: 97 1523 BciVI GTATCCnnnnn_n' Cuts at: 0 1083 1620 Size: 1083 537 BglI GCCn_nnn'nGGC Cuts at: 0 1195 1620 Size: 1195 425 Bme1580I G_kGCm'C Cuts at: 0 911 1620 Size: 911 709 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 369 1587 1620 Size: 369 1218 33 Fragments arranged by size: 1218 369 33 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 343 503 658 1042 1553 1598 1620 Size: 343 160 155 384 511 45 22 Fragments arranged by size: 511 384 343 160 155 45 22 Bpu10I CC'TnA_GC Cuts at: 0 97 1620 Size: 97 1523 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 904 1620 Size: 904 716 BsaI GGTCTCn'nnnn_ Cuts at: 0 616 1436 1620 Size: 616 820 184 Fragments arranged by size: 820 616 184 BsaWI w'CCGG_w Cuts at: 0 461 1620 Size: 461 1159 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 166 196 781 811 1015 1045 1620 Size: 166 30 585 30 204 30 575 Fragments arranged by size: 585 575 204 166 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 174 1399 1402 1620 Size: 174 1225 3 218 Fragments arranged by size: 1225 218 174 3 BseYI C'CCAG_C Cuts at: 0 443 476 824 853 1162 1620 Size: 443 33 348 29 309 458 Fragments arranged by size: 458 443 348 309 33 29 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 244 759 1400 1620 Size: 244 515 641 220 Fragments arranged by size: 641 515 244 220 BsiHKAI G_wGCw'C Cuts at: 0 110 1620 Size: 110 1510 BsmI GAATG_Cn' Cuts at: 0 392 441 1620 Size: 392 49 1179 Fragments arranged by size: 1179 392 49 Bsp1286I G_dGCh'C Cuts at: 0 110 911 1200 1573 1610 1620 Size: 110 801 289 373 37 10 Fragments arranged by size: 801 373 289 110 37 10 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 285 980 1361 1620 Size: 285 695 381 259 Fragments arranged by size: 695 381 285 259 BsrBI CCG'CTC Cuts at: 0 956 1620 Size: 956 664 BsrDI GCAATG_nn' Cuts at: 0 725 1620 Size: 725 895 BsrFI r'CCGG_y Cuts at: 0 461 485 1620 Size: 461 24 1135 Fragments arranged by size: 1135 461 24 BsrGI T'GTAC_A Cuts at: 0 1069 1620 Size: 1069 551 BstXI CCAn_nnnn'nTGG Cuts at: 0 1263 1276 1620 Size: 1263 13 344 Fragments arranged by size: 1263 344 13 Bsu36I CC'TnA_GG Cuts at: 0 320 943 1620 Size: 320 623 677 Fragments arranged by size: 677 623 320 BtgI C'CryG_G Cuts at: 0 167 1620 Size: 167 1453 BtsI GCAGTG_nn' Cuts at: 0 447 663 1620 Size: 447 216 957 Fragments arranged by size: 957 447 216 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1129 1164 1620 Size: 1129 35 456 Fragments arranged by size: 1129 456 35 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 343 503 658 1042 1553 1598 1620 Size: 343 160 155 384 511 45 22 Fragments arranged by size: 511 384 343 160 155 45 22 EcoO109I rG'GnC_Cy Cuts at: 0 307 1620 Size: 307 1313 HaeII r_GCGC'y Cuts at: 0 951 1041 1620 Size: 951 90 579 Fragments arranged by size: 951 579 90 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 859 891 1418 1450 1620 Size: 859 32 527 32 170 Fragments arranged by size: 859 527 170 32 32 HincII GTy'rAC Cuts at: 0 1488 1620 Size: 1488 132 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 282 1620 Size: 282 1338 MslI CAynn'nnrTG Cuts at: 0 368 1274 1620 Size: 368 906 346 Fragments arranged by size: 906 368 346 MspA1I CmG'CkG Cuts at: 0 96 656 1020 1343 1620 Size: 96 560 364 323 277 Fragments arranged by size: 560 364 323 277 96 NcoI C'CATG_G Cuts at: 0 167 1620 Size: 167 1453 NspI r_CATG'y Cuts at: 0 562 1620 Size: 562 1058 PasI CC'CwG_GG Cuts at: 0 505 1620 Size: 505 1115 PflMI CCAn_nnn'nTGG Cuts at: 0 489 1620 Size: 489 1131 PfoI T'CCnGG_A Cuts at: 0 430 635 809 1620 Size: 430 205 174 811 Fragments arranged by size: 811 430 205 174 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 727 759 781 813 1015 1047 1620 Size: 727 32 22 32 202 32 573 Fragments arranged by size: 727 573 202 32 32 32 22 PpuMI rG'GwC_Cy Cuts at: 0 307 1620 Size: 307 1313 PshAI GACnn'nnGTC Cuts at: 0 1241 1620 Size: 1241 379 PvuII CAG'CTG Cuts at: 0 96 1020 1620 Size: 96 924 600 Fragments arranged by size: 924 600 96 SanDI GG'GwC_CC Cuts at: 0 307 1620 Size: 307 1313 SfcI C'TryA_G Cuts at: 0 80 1620 Size: 80 1540 SmlI C'TyrA_G Cuts at: 0 883 1620 Size: 883 737 StuI AGG'CCT Cuts at: 0 1081 1372 1620 Size: 1081 291 248 Fragments arranged by size: 1081 291 248 StyI C'CwwG_G Cuts at: 0 23 167 468 1550 1610 1620 Size: 23 144 301 1082 60 10 Fragments arranged by size: 1082 301 144 60 23 10 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 738 1620 Size: 738 882 TatI w'GTAC_w Cuts at: 0 1069 1620 Size: 1069 551 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 166 198 1620 Size: 166 32 1422 Fragments arranged by size: 1422 166 32 Tth111I GACn'n_nGTC Cuts at: 0 215 1620 Size: 215 1405 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 21 490 1620 Size: 21 469 1130 Fragments arranged by size: 1130 469 21 Enzymes that do cut: AfeI AgeI AleI AloI AlwNI AvaI BanI BanII BbsI BbvCI BciVI BglI Bme1580I BplI BpmI Bpu10I BpuEI BsaI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrGI BstXI Bsu36I BtgI BtsI CspCI Eco57MI EcoO109I HaeII Hin4I HincII MmeI MslI MspA1I NcoI NspI PasI PflMI PfoI PpiI PpuMI PshAI PvuII SanDI SfcI SmlI StuI StyI TaqII TatI TstI Tth111I XcmI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AhdI AlfI ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI BcgI BclI BfrBI BglII BlpI BmgBI BmrI BmtI BsaAI BsaBI BsaHI BsiEI BsiWI BsmBI BspEI BspHI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgZI ClaI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HindIII HpaI KasI KpnI MfeI MluI MscI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI PacI PciI PmeI PmlI PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI XbaI XhoI XmaI XmnI ZraI