################################## # NO CUT ENZYMES ( 122 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AflII AflIII AgeI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AvaI AviII AvrII BalI BamHI BanII BbrPI BbsI BcgI BciVI BclI BfrI BglI BglII BlnI BpuAI BsaAI BsaBI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I ClaI CpoI DraI DraIII DrdI EagI EclXI Eco47III Eco52I EcoNI EcoO65I EcoRV EcoT22I FbaI FspI HgaI HindIII HpaI KpnI KspI MamI MfeI MluI MluNI MroI MscI MunI MvnI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SbfI ScaI SexAI SfaNI SfiI SfuI SmaI SnaBI SpeI Sse8387I SspBI SspI SwaI XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AcsI 1435 GAATTC [GA]^AATT[CT] AcyI 404 GGCGCC G[GA]^CG[CT]C AfaI 1015 GTAC GT^AC AhdI 89 GACCTCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 91 CCTCAGG CC^T[ACGT]AGG ApoI 1435 GAATTC [GA]^AATT[CT] AspEI 89 GACCTCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 958 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC BanI 404 GGCGCC G^G[CT][GA]CC BbeI 404 GGCGCC GGCGC^C BbvCI 499 GCTGAGG CCTCAGC(-5/-2) BsaHI 404 GGCGCC G[GA]^CG[CT]C BsmI 744 GAATGC GAATGC(1/-1) BspMI 1055 GCAGGT ACCTGC(4/8) BsrBI 258 GAGCGG CCGCTC(-3/-3) Bsu36I 91 CCTCAGG CC^T[ACGT]AGG Eam1105I 89 GACCTCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 91 CCTCAGG CC^T[ACGT]AGG EcoRI 1435 GAATTC G^AATTC FseI 313 GGCCGGCC GGCCGG^CC Hin1I 404 GGCGCC G[GA]^CG[CT]C KasI 404 GGCGCC G^GCGCC MslI 240 CACACCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MwoI 1065 GCGGCCCCGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NarI 404 GGCGCC GG^CGCC NcoI 127 CCATGG C^CATGG PflFI 958 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 373 CCAGACTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 376 GACTC GAGTC(4/5) PshAI 582 GACCAATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 220 CTGCAG CTGCA^G RsaI 1015 GTAC GT^AC SapI 478 GAAGAGC GCTCTTC(1/4) SfoI 404 GGCGCC GGC^GCC SgrAI 400 CGCCGGCG C[GA]^CCGG[CT]G SmlI 84 CTCAAG C^T[CT][GA]AG SphI 1497 GCATGC GCATG^C TaqI 1316 TCGA T^CGA TfiI 114 GAATC G^A[AT]TC Tth111I 958 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 1316 TCGA T^CGA Van91I 373 CCAGACTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### BcnI 834 CCGGG CC^[GC]GG 1070 CCCGG BfaI 649 CTAG C^TAG 1039 CTAG BlpI 255 GCTGAGC GC^T[ACGT]AGC 642 GCTTAGC BpmI 981 CTGGAG CTGGAG(16/14) 1117 CTGGAG Bpu1102I 255 GCTGAGC GC^T[ACGT]AGC 642 GCTTAGC BsaI 1120 GAGACC GGTCTC(1/5) 1378 GAGACC BseRI 456 CTCCTC GAGGAG(10/8) 1033 GAGGAG BsrDI 438 CATTGC GCAATG(2/0) 1383 CATTGC CelII 255 GCTGAGC GC^T[ACGT]AGC 642 GCTTAGC EaeI 23 CGGCCA [CT]^GGCC[GA] 609 TGGCCG EcoT14I 127 CCATGG C^C[AT][AT]GG 1205 CCAAGG HaeII 404 GGCGCC [GA]GCGC^[CT] 614 GGCGCT HincII 652 GTCAAC GT[CT]^[GA]AC 1264 GTCAAC HindII 652 GTCAAC GT[CT]^[GA]AC 1264 GTCAAC HinfI 114 GAATC G^A[ACGT]TC 376 GACTC MaeI 649 CTAG C^TAG 1039 CTAG MaeII 215 ACGT A^CGT 517 ACGT NciI 834 CCGGG CC^[GC]GG 1070 CCCGG StuI 105 AGGCCT AGG^CCT 1106 AGGCCT StyI 127 CCATGG C^C[AT][AT]GG 1205 CCAAGG XcmI 118 CCACCAGGGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 239 CCACACCCGTGGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 73 enzymes) # ######################################### AciI AluI AlwI AlwNI Asp700I AspHI AvaII BbvI BmyI BsaJI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrFI BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EarI EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HpaII HphI ItaI Ksp632I MaeIII MboI MboII MflI MnlI MseI MspA1I MspI MvaI NaeI NdeII NgoMIV NlaIII NlaIV NspI PpuMI PvuII Sau3AI Sau96I ScrFI SfcI Tru9I TseI Tsp45I Tsp509I TspRI XhoII XmnI