(Circular) (Six-base) MAPSORT of: pFN21AE4491.seq Check: 5956 from: 1 to: 6241 pFN21AE4491 6241 bp With 182 enzymes: SgfI * March 28, 2012 13:58 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4051 278 Size: 53 83 186 936 2515 2468 Fragments arranged by size: 2515 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3584 1937 Size: 1647 4594 Acc65I G'GTAC_C Cuts at: 3562 3562 Size: 6241 AclI AA'CG_TT Cuts at: 1563 4368 4741 1563 Size: 2805 373 3063 Fragments arranged by size: 3063 2805 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2135 2356 4304 5688 5956 1632 Size: 503 221 1948 1384 268 1917 Fragments arranged by size: 1948 1917 1384 503 268 221 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6044 AflIII A'CryG_T Cuts at: 1203 5140 6117 1203 Size: 3937 977 1327 Fragments arranged by size: 3937 1327 977 AgeI A'CCGG_T Cuts at: 5072 5072 Size: 6241 AhdI GACnn_n'nnGTC Cuts at: 2099 4970 2099 Size: 2871 3370 AleI CACnn'nnGTG Cuts at: 1166 2249 1166 Size: 1083 5158 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6241 AlwNI CAG_nnn'CTG Cuts at: 2290 2384 2533 2546 3984 5556 2290 Size: 94 149 13 1438 1572 2975 Fragments arranged by size: 2975 1572 1438 149 94 13 ApaLI G'TGCA_C Cuts at: 4298 5454 4298 Size: 1156 5085 ApoI r'AATT_y Cuts at: 1493 3440 3550 3776 3928 6037 1493 Size: 1947 110 226 152 2109 1697 Fragments arranged by size: 2109 1947 1697 226 152 110 AseI AT'TA_AT Cuts at: 160 1032 4795 160 Size: 872 3763 1606 Fragments arranged by size: 3763 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6241 AvaI C'yCGr_G Cuts at: 1870 1943 3566 1870 Size: 73 1623 4545 Fragments arranged by size: 4545 1623 73 BamHI G'GATC_C Cuts at: 3571 3938 3571 Size: 367 5874 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2409 3562 5017 618 Size: 325 217 627 622 1153 1455 1842 Fragments arranged by size: 1842 1455 1153 627 622 325 217 BanII G_rGCy'C Cuts at: 1391 3560 1391 Size: 2169 4072 BbeI G_GCGC'C Cuts at: 2413 2413 Size: 6241 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5270 BbvCI CC'TCA_GC Cuts at: 2505 2505 Size: 6241 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4432 4466 1903 Size: 34 50 34 2411 34 3678 Fragments arranged by size: 3678 2411 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4133 5343 4133 Size: 1210 5031 BclI T'GATC_A Cuts at: 1548 1548 Size: 6241 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4852 136 Size: 107 122 71 631 605 3180 1525 Fragments arranged by size: 3180 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6236 6236 Size: 6241 BlpI GC'TnA_GC Cuts at: 2261 2648 3658 2261 Size: 387 1010 4844 Fragments arranged by size: 4844 1010 387 Bme1580I G_kGCm'C Cuts at: 1790 2157 2553 4302 5458 1790 Size: 367 396 1749 1156 2573 Fragments arranged by size: 2573 1749 1156 396 367 BmgBI CAC'GTC Cuts at: 1318 2221 1318 Size: 903 5338 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3464 3972 4930 Size: 416 53 83 399 24 2047 508 958 Cuts at: 4930 442 Size: 1753 Fragments arranged by size: 2047 1753 958 508 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6241 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3007 3143 4901 1239 Size: 1768 136 1758 2579 Fragments arranged by size: 2579 1768 1758 136 Bpu10I CC'TnA_GC Cuts at: 2505 2505 Size: 6241 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2074 4366 5231 5529 2074 Size: 2292 865 298 2786 Fragments arranged by size: 2786 2292 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3119 3377 4904 882 Size: 617 1620 258 1527 2219 Fragments arranged by size: 2219 1620 1527 617 258 BsaAI yAC'GTr Cuts at: 493 1204 4032 493 Size: 711 2828 2702 Fragments arranged by size: 2828 2702 711 BsaBI GATnn'nnATC Cuts at: 6235 6235 Size: 6241 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2410 4048 4430 5065 Size: 53 83 186 936 877 1638 382 635 Cuts at: 5065 275 Size: 1451 Fragments arranged by size: 1638 1451 936 877 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4674 5072 5346 5493 5904 4674 Size: 398 274 147 411 5011 Fragments arranged by size: 5011 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2444 2474 1178 Size: 30 165 30 1041 30 4945 Fragments arranged by size: 4945 1041 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2452 3053 1186 Size: 1266 601 4374 Fragments arranged by size: 4374 1266 601 BseYI C'CCAG_C Cuts at: 2455 2701 2954 3396 5444 5940 2455 Size: 246 253 442 2048 496 2756 Fragments arranged by size: 2756 2048 496 442 253 246 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5668 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3691 4452 5480 1540 Size: 275 186 1690 761 1028 2301 Fragments arranged by size: 2301 1690 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2811 2868 3519 4302 4387 5458 2811 Size: 57 651 783 85 1071 3594 Fragments arranged by size: 3594 1071 783 651 85 57 BsmI GAATG_Cn' Cuts at: 2755 3752 3845 2755 Size: 997 93 5151 Fragments arranged by size: 5151 997 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2157 2553 2811 2868 3519 3560 4302 Size: 399 367 396 258 57 651 41 742 Cuts at: 4302 4387 5458 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 651 399 396 367 258 85 57 41 BspEI T'CCGG_A Cuts at: 5904 5904 Size: 6241 BspHI T'CATG_A Cuts at: 4130 5860 4130 Size: 1730 4511 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3051 3596 844 Size: 2207 545 3489 Fragments arranged by size: 3489 2207 545 BsrBI CCG'CTC Cuts at: 1394 1823 2265 4128 1394 Size: 429 442 1863 3507 Fragments arranged by size: 3507 1863 442 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2442 3387 4736 4910 65 Size: 1183 1194 945 1349 174 1396 Fragments arranged by size: 1396 1349 1194 1183 945 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2319 2406 2616 4885 5072 1700 Size: 110 509 87 210 2269 187 2869 Fragments arranged by size: 2869 2269 509 210 187 110 87 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6241 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6241 BssSI C'ACGA_G Cuts at: 4301 5313 4301 Size: 1012 5229 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6241 BstBI TT'CG_AA Cuts at: 5045 5045 Size: 6241 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6241 BstXI CCAn_nnnn'nTGG Cuts at: 2133 2139 2213 2251 2133 Size: 6 74 38 6123 Fragments arranged by size: 6123 74 38 6 BstYI r'GATC_y Cuts at: 1972 2229 3030 3089 3571 3938 4323 4340 5792 Size: 257 801 59 482 367 385 17 1452 Cuts at: 5792 5878 5890 6236 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 801 482 385 367 346 257 86 59 17 12 Bsu36I CC'TnA_GG Cuts at: 2097 2097 Size: 6241 BtgI C'CryG_G Cuts at: 513 1065 2031 2132 2250 513 Size: 552 966 101 118 4504 Fragments arranged by size: 4504 966 552 118 101 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2319 487 Size: 683 56 1093 4409 Fragments arranged by size: 4409 1093 683 56 BtsI GCAGTG_nn' Cuts at: 3757 4551 4571 3757 Size: 794 20 5427 Fragments arranged by size: 5427 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6241 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6206 DraI TTT'AAA Cuts at: 3545 3545 Size: 6241 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5248 809 Size: 507 3932 1802 Fragments arranged by size: 3932 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2028 2614 3688 Size: 54 1425 50 275 18 198 586 1074 Cuts at: 3688 4577 6206 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1074 889 586 275 198 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3688 1537 Size: 275 1876 4090 Fragments arranged by size: 4090 1876 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2478 3145 3416 4171 1353 Size: 1125 667 271 755 3423 Fragments arranged by size: 3423 1125 755 667 271 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4828 5202 5348 387 Size: 1064 138 3239 374 146 1280 Fragments arranged by size: 3239 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2135 2356 3007 3143 4304 4901 5688 Size: 393 503 221 651 136 1161 597 787 Cuts at: 5688 5956 1239 Size: 268 1524 Fragments arranged by size: 1524 1161 787 651 597 503 393 268 221 136 EcoO109I rG'GnC_Cy Cuts at: 2085 2101 2287 2509 2543 2761 2085 Size: 16 186 222 34 218 5565 Fragments arranged by size: 5565 222 218 186 34 16 EcoRI G'AATT_C Cuts at: 3440 3550 3928 3440 Size: 110 378 5753 Fragments arranged by size: 5753 378 110 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2663 2663 Size: 6241 FseI GG_CCGG'CC Cuts at: 2323 2323 Size: 6241 FspI TGC'GCA Cuts at: 4747 4747 Size: 6241 HaeII r_GCGC'y Cuts at: 1678 2413 2623 5388 1678 Size: 735 210 2765 2531 Fragments arranged by size: 2765 2531 735 210 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2085 2117 2444 2476 4882 Size: 32 135 32 513 32 327 32 2406 Cuts at: 4882 4914 4956 4988 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2406 513 327 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2659 3271 3585 3831 669 Size: 988 281 721 612 314 246 3079 Fragments arranged by size: 3079 988 721 612 314 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6241 HpaI GTT'AAC Cuts at: 3831 3831 Size: 6241 KasI G'GCGC_C Cuts at: 2409 2409 Size: 6241 KpnI G_GTAC'C Cuts at: 3566 3566 Size: 6241 MfeI C'AATT_G Cuts at: 3840 3840 Size: 6241 MluI A'CGCG_T Cuts at: 6117 6117 Size: 6241 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3157 5355 5539 1305 Size: 1852 2198 184 2007 Fragments arranged by size: 2198 2007 1852 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4419 Fragments arranged by size: 4419 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2249 4199 4558 4717 518 Size: 648 1083 1950 359 159 2042 Fragments arranged by size: 2042 1950 1083 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2705 2884 2908 3396 3532 3657 4334 5482 Size: 1099 179 24 488 136 125 677 1148 Cuts at: 5482 5727 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 1099 677 488 245 179 136 125 24 NaeI GCC'GGC Cuts at: 2321 2408 2618 2321 Size: 87 210 5944 Fragments arranged by size: 5944 210 87 NarI GG'CG_CC Cuts at: 2410 2410 Size: 6241 NcoI C'CATG_G Cuts at: 513 1065 2132 513 Size: 552 1067 4622 Fragments arranged by size: 4622 1067 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6241 NgoMIV G'CCGG_C Cuts at: 2319 2406 2616 2319 Size: 87 210 5944 Fragments arranged by size: 5944 210 87 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6241 NotI GC'GGCC_GC Cuts at: 3688 3688 Size: 6241 NspI r_CATG'y Cuts at: 1132 1229 2161 2368 2492 3506 3599 5144 1132 Size: 97 932 207 124 1014 93 1545 2229 Fragments arranged by size: 2229 1545 1014 932 207 124 97 93 PasI CC'CwG_GG Cuts at: 1348 2167 3015 1348 Size: 819 848 4574 Fragments arranged by size: 4574 848 819 PciI A'CATG_T Cuts at: 5140 5140 Size: 6241 PflMI CCAn_nnn'nTGG Cuts at: 1108 2384 1108 Size: 1276 4965 PfoI T'CCnGG_A Cuts at: 2838 2984 3120 2838 Size: 146 136 5959 Fragments arranged by size: 5959 146 136 PmeI GTTT'AAAC Cuts at: 3545 3545 Size: 6241 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3285 3317 4310 4342 5823 5855 3285 Size: 32 993 32 1481 32 3671 Fragments arranged by size: 3671 1481 993 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2101 2509 2761 2101 Size: 408 252 5581 Fragments arranged by size: 5581 408 252 PshAI GACnn'nnGTC Cuts at: 1613 2591 1613 Size: 978 5263 PsiI TTA'TAA Cuts at: 3811 3811 Size: 6241 PstI C_TGCA'G Cuts at: 830 2229 3593 830 Size: 1399 1364 3478 Fragments arranged by size: 3478 1399 1364 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6241 PvuII CAG'CTG Cuts at: 2705 2884 2908 3532 2705 Size: 179 24 624 5414 Fragments arranged by size: 5414 624 179 24 SalI G'TCGA_C Cuts at: 1936 3583 1936 Size: 1647 4594 SapI GCTCTTCn'nnn_ Cuts at: 2478 2478 Size: 6241 SbfI CC_TGCA'GG Cuts at: 3593 3593 Size: 6241 ScaI AGT'ACT Cuts at: 4489 6201 4489 Size: 1712 4529 SfcI C'TryA_G Cuts at: 826 1045 2225 2563 2779 3589 4724 5405 5596 Size: 219 1180 338 216 810 1135 681 191 Cuts at: 5596 826 Size: 1471 Fragments arranged by size: 1471 1180 1135 810 681 338 219 216 191 SfoI GGC'GCC Cuts at: 2411 2411 Size: 6241 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6241 SgrAI Cr'CCGG_yG Cuts at: 1700 2406 1700 Size: 706 5535 SmaI CCC'GGG Cuts at: 1872 3568 1872 Size: 1696 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2089 4345 5246 5508 820 Size: 197 926 146 2256 901 262 1553 Fragments arranged by size: 2256 1553 926 901 262 197 146 SnaBI TAC'GTA Cuts at: 493 4032 493 Size: 3539 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6241 SphI G_CATG'C Cuts at: 1132 3506 3599 1132 Size: 2374 93 3774 Fragments arranged by size: 3774 2374 93 SspI AAT'ATT Cuts at: 5 52 4165 5 Size: 47 4113 2081 Fragments arranged by size: 4113 2081 47 StuI AGG'CCT Cuts at: 1517 2112 3113 1517 Size: 595 1001 4645 Fragments arranged by size: 4645 1001 595 StyI C'CwwG_G Cuts at: 513 1065 2132 3210 3680 513 Size: 552 1067 1078 470 3074 Fragments arranged by size: 3074 1078 1067 552 470 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4268 4285 4438 4623 5068 325 Size: 1255 2688 17 153 185 445 1498 Fragments arranged by size: 2688 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4487 6199 96 Size: 274 80 33 51 1443 2510 1712 138 Fragments arranged by size: 2510 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6201 6233 6201 Size: 32 6209 Tth111I GACn'n_nGTC Cuts at: 1316 2966 1316 Size: 1650 4591 XbaI T'CTAG_A Cuts at: 3577 3577 Size: 6241 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2130 2251 2130 Size: 121 6120 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6241 XmaI C'CCGG_G Cuts at: 1870 3566 1870 Size: 1696 4545 XmnI GAAnn'nnTTC Cuts at: 2435 2753 3297 4370 2435 Size: 318 544 1073 4306 Fragments arranged by size: 4306 1073 544 318 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4049 276 Size: 53 83 186 936 2515 2468 Fragments arranged by size: 2515 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV FspAI NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SrfI SwaI