################################## # NO CUT ENZYMES ( 170 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AfaI AflII AflIII AgeI AhdI AocI ApaI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BalI BamHI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfrI BglI BglII BlnI BpmI BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsiEI BsiWI BsmAI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstF5I BstPI BstXI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI DraIII EaeI EagI Eam1105I EarI EclXI Eco52I Eco81I EcoNI EcoO65I EcoRV EcoT14I EcoT22I FbaI FokI FseI FspI HgaI Hin1I HincII HindII HindIII HpaI HphI KasI KpnI Ksp632I KspI MaeII MaeIII MamI MboII MfeI MluI MluNI MroI MscI MseI MslI MunI NaeI NarI NcoI NdeI NgoMIV NheI NlaIII NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RcaI RsaI RsrII SacII SalI SapI SbfI ScaI SexAI SfaNI SfcI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI Tru9I Tsp45I Tth111I TthHB8I Van91I XcmI XhoI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AccII 297 CGCG CG^CG AcsI 87 GAATTC [GA]^AATT[CT] Alw44I 156 GTGCAC G^TGCAC AlwI 279 GATCC GGATC(4/5) Aor51HI 400 AGCGCT AGC^GCT ApaLI 156 GTGCAC G^TGCAC ApoI 87 GAATTC [GA]^AATT[CT] Asp700I 342 GAACTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BanI 378 GGCACC G^G[CT][GA]CC BlpI 189 GCTGAGC GC^T[ACGT]AGC Bpu1102I 189 GCTGAGC GC^T[ACGT]AGC BsgI 307 GTGCAG GTGCAG(16/14) BspMI 323 ACCTGC ACCTGC(4/8) BstUI 297 CGCG CG^CG BstYI 278 AGATCC [GA]^GATC[CT] CelII 189 GCTGAGC GC^T[ACGT]AGC DdeI 190 CTGAG C^T[ACGT]AG DpnII 279 GATC ^GATC DrdI 204 GACCTCGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 390 CCACGG C^C[GA][CT]GG Eco47III 400 AGCGCT AGC^GCT EcoRI 87 GAATTC G^AATTC HaeII 400 AGCGCT [GA]GCGC^[CT] HinfI 115 GAATC G^A[ACGT]TC MboI 279 GATC ^GATC MflI 278 AGATCC [GA]^GATC[CT] MvnI 297 CGCG CG^CG NdeII 279 GATC ^GATC PvuII 173 CAGCTG CAG^CTG SacI 228 GAGCTC GAGCT^C Sau3AI 279 GATC ^GATC SmaI 136 CCCGGG CCC^GGG TfiI 115 GAATC G^A[AT]TC Tsp509I 88 AATT ^AATT TspRI 8 CACTG CA[GC]TG[ACGT][ACGT]^ XbaI 91 TCTAGA T^CTAGA XhoII 278 AGATCC [GA]^GATC[CT] XmaCI 136 CCCGGG C^CCGGG XmaI 136 CCCGGG C^CCGGG XmnI 342 GAACTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AlwNI 22 CAGCTACTG CAG[ACGT][ACGT][ACGT]^CTG 145 CAGAGCCTG AspHI 156 GTGCAC G[AT]GC[AT]^C 228 GAGCTC AvaI 136 CCCGGG C^[CT]CG[GA]G 223 CCCGAG AvaII 203 GGACC G^G[AT]CC 321 GGACC BanII 228 GAGCTC G[GA]GC[CT]^C 237 GAGCCC BfaI 92 CTAG C^TAG 112 CTAG BsiHKAI 156 GTGCAC G[AT]GC[AT]^C 228 GAGCTC BsmFI 202 GGGAC GGGAC(10/14) 213 GTCCC BsoBI 136 CCCGGG C^[CT]CG[GA]G 223 CCCGAG BsrI 9 ACTGG ACTGG(1/-1) 338 ACTGG BstAPI 21 GCAGCTACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 144 GCAGAGCCTGC DraII 202 GGGACCT [GA]G^G[ACGT]CC[CT] 320 AGGACCT EcoO109I 202 GGGACCT [GA]G^G[ACGT]CC[CT] 320 AGGACCT MaeI 92 CTAG C^TAG 112 CTAG MspA1I 173 CAGCTG C[AC]G^C[GT]G 310 CAGCGG PpuMI 202 GGGACCT [GA]G^G[AT]CC[CT] 320 AGGACCT ######################################### # 3 OR MORE CUT ENZYMES ( 32 enzymes) # ######################################### AciI AluI BbvI BcnI BmyI BsaJI BsiYI BslI Bsp1286I BssKI BstNI Cac8I CfoI Cfr13I EcoRII FauI Fnu4HI HaeIII HapII HhaI HinP1I HpaII ItaI MnlI MspI MvaI MwoI NciI NlaIV Sau96I ScrFI TseI