(Linear) (Six-base) MAPSORT of: KIEE5108.seq Check: 25 from: 1 to: 414 KIEE5108.orf With 182 enzymes: SgfI * January 29, 2014 11:46 .. AfeI AGC'GCT Cuts at: 0 402 414 Size: 402 12 AlwNI CAG_nnn'CTG Cuts at: 0 27 150 414 Size: 27 123 264 Fragments arranged by size: 264 123 27 ApaLI G'TGCA_C Cuts at: 0 156 414 Size: 156 258 ApoI r'AATT_y Cuts at: 0 87 414 Size: 87 327 AvaI C'yCGr_G Cuts at: 0 136 223 414 Size: 136 87 191 Fragments arranged by size: 191 136 87 BanI G'GyrC_C Cuts at: 0 378 414 Size: 378 36 BanII G_rGCy'C Cuts at: 0 232 241 414 Size: 232 9 173 Fragments arranged by size: 232 173 9 BlpI GC'TnA_GC Cuts at: 0 190 414 Size: 190 224 Bme1580I G_kGCm'C Cuts at: 0 160 414 Size: 160 254 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 328 358 414 Size: 328 30 56 Fragments arranged by size: 328 56 30 BseYI C'CCAG_C Cuts at: 0 242 403 414 Size: 242 161 11 Fragments arranged by size: 242 161 11 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 328 414 Size: 328 86 BsiHKAI G_wGCw'C Cuts at: 0 160 232 414 Size: 160 72 182 Fragments arranged by size: 182 160 72 Bsp1286I G_dGCh'C Cuts at: 0 160 232 241 414 Size: 160 72 9 173 Fragments arranged by size: 173 160 72 9 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 332 414 Size: 332 82 BstAPI GCAn_nnn'nTGC Cuts at: 0 27 150 414 Size: 27 123 264 Fragments arranged by size: 264 123 27 BstYI r'GATC_y Cuts at: 0 278 414 Size: 278 136 BtgI C'CryG_G Cuts at: 0 390 414 Size: 390 24 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 33 68 414 Size: 33 35 346 Fragments arranged by size: 346 35 33 DrdI GACnn_nn'nnGTC Cuts at: 0 210 414 Size: 210 204 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 249 414 Size: 249 165 EcoICRI GAG'CTC Cuts at: 0 230 414 Size: 230 184 EcoO109I rG'GnC_Cy Cuts at: 0 203 321 414 Size: 203 118 93 Fragments arranged by size: 203 118 93 EcoRI G'AATT_C Cuts at: 0 87 414 Size: 87 327 HaeII r_GCGC'y Cuts at: 0 404 414 Size: 404 10 MspA1I CmG'CkG Cuts at: 0 175 312 414 Size: 175 137 102 Fragments arranged by size: 175 137 102 PasI CC'CwG_GG Cuts at: 0 120 291 414 Size: 120 171 123 Fragments arranged by size: 171 123 120 PfoI T'CCnGG_A Cuts at: 0 232 414 Size: 232 182 PpuMI rG'GwC_Cy Cuts at: 0 203 321 414 Size: 203 118 93 Fragments arranged by size: 203 118 93 PvuII CAG'CTG Cuts at: 0 175 414 Size: 175 239 SacI G_AGCT'C Cuts at: 0 232 414 Size: 232 182 SmaI CCC'GGG Cuts at: 0 138 414 Size: 138 276 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 61 414 Size: 61 353 XbaI T'CTAG_A Cuts at: 0 91 414 Size: 91 323 XmaI C'CCGG_G Cuts at: 0 136 414 Size: 136 278 XmnI GAAnn'nnTTC Cuts at: 0 346 414 Size: 346 68 Enzymes that do cut: AfeI AlwNI ApaLI ApoI AvaI BanI BanII BlpI Bme1580I BsaXI BseYI BsgI BsiHKAI Bsp1286I BspMI BstAPI BstYI BtgI CspCI DrdI EciI EcoICRI EcoO109I EcoRI HaeII MspA1I PasI PfoI PpuMI PvuII SacI SmaI TaqII XbaI XmaI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI ApaI AscI AseI AsiSI AvrII BaeI BamHI BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI BtsI ClaI DraI DraIII EaeI EagI EarI Eco57MI EcoNI EcoRV FalI FseI FspI FspAI Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI SwaI TatI TstI Tth111I XcmI XhoI ZraI