(Circular) (Six-base) MAPSORT of: pFN21AB7990.seq Check: 706 from: 1 to: 5683 pFN21AB7990 5683 bp With 182 enzymes: SgfI * April 15, 2010 09:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2824 2824 Size: 5683 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3493 278 Size: 53 83 186 936 1957 2468 Fragments arranged by size: 2468 1957 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3026 1937 Size: 1089 4594 Acc65I G'GTAC_C Cuts at: 3004 3004 Size: 5683 AclI AA'CG_TT Cuts at: 1563 3810 4183 1563 Size: 2247 373 3063 Fragments arranged by size: 3063 2247 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2388 3746 5130 5398 1632 Size: 756 1358 1384 268 1917 Fragments arranged by size: 1917 1384 1358 756 268 AfeI AGC'GCT Cuts at: 2732 2732 Size: 5683 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5486 AflIII A'CryG_T Cuts at: 1203 4582 5559 1203 Size: 3379 977 1327 Fragments arranged by size: 3379 1327 977 AgeI A'CCGG_T Cuts at: 4514 4514 Size: 5683 AhdI GACnn_n'nnGTC Cuts at: 2038 4412 2038 Size: 2374 3309 AleI CACnn'nnGTG Cuts at: 1166 2330 1166 Size: 1164 4519 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2440 1184 Size: 1256 4427 AlwNI CAG_nnn'CTG Cuts at: 2182 2615 3426 4998 2182 Size: 433 811 1572 2867 Fragments arranged by size: 2867 1572 811 433 ApaLI G'TGCA_C Cuts at: 3740 4896 3740 Size: 1156 4527 ApoI r'AATT_y Cuts at: 1493 2108 2992 3218 3370 5479 1493 Size: 615 884 226 152 2109 1697 Fragments arranged by size: 2109 1697 884 615 226 152 AseI AT'TA_AT Cuts at: 160 1032 4237 160 Size: 872 3205 1606 Fragments arranged by size: 3205 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5683 AvaI C'yCGr_G Cuts at: 1870 1943 2021 2043 2588 3008 1870 Size: 73 78 22 545 420 4545 Fragments arranged by size: 4545 545 420 78 73 22 BamHI G'GATC_C Cuts at: 3013 3380 3013 Size: 367 5316 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2324 2831 3004 4459 618 Size: 325 217 627 537 507 173 1455 1842 Fragments arranged by size: 1842 1455 627 537 507 325 217 173 BanII G_rGCy'C Cuts at: 1391 2679 2784 3002 1391 Size: 1288 105 218 4072 Fragments arranged by size: 4072 1288 218 105 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2839 928 Size: 971 940 3772 Fragments arranged by size: 3772 971 940 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3874 3908 1903 Size: 34 1937 34 3678 Fragments arranged by size: 3678 1937 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3575 4785 3575 Size: 1210 4473 BclI T'GATC_A Cuts at: 1548 2208 2310 1548 Size: 660 102 4921 Fragments arranged by size: 4921 660 102 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4294 136 Size: 107 122 71 631 605 2622 1525 Fragments arranged by size: 2622 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5678 5678 Size: 5683 BlpI GC'TnA_GC Cuts at: 2517 3100 2517 Size: 583 5100 Bme1580I G_kGCm'C Cuts at: 1790 2834 3744 4900 1790 Size: 1044 910 1156 2573 Fragments arranged by size: 2573 1156 1044 910 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5683 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2564 3414 4372 Size: 416 53 83 399 24 1147 850 958 Cuts at: 4372 442 Size: 1753 Fragments arranged by size: 1753 1147 958 850 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5683 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2098 2098 Size: 5683 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2068 2501 2564 2888 4343 1239 Size: 829 433 63 324 1455 2579 Fragments arranged by size: 2579 1455 829 433 324 63 Bpu10I CC'TnA_GC Cuts at: 2131 2131 Size: 5683 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3808 4673 4971 3808 Size: 865 298 4520 Fragments arranged by size: 4520 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4346 882 Size: 617 2847 2219 Fragments arranged by size: 2847 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3474 493 Size: 711 2270 2702 Fragments arranged by size: 2702 2270 711 BsaBI GATnn'nnATC Cuts at: 5677 5677 Size: 5683 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2437 3490 3872 4507 Size: 53 83 186 936 904 1053 382 635 Cuts at: 4507 275 Size: 1451 Fragments arranged by size: 1451 1053 936 904 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4116 4514 4788 4935 5346 4116 Size: 398 274 147 411 4453 Fragments arranged by size: 4453 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2477 2507 2537 2567 2861 Size: 30 165 30 1074 30 30 30 294 Cuts at: 2861 2891 1178 Size: 30 3970 Fragments arranged by size: 3970 1074 294 165 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2359 2387 2390 2489 2498 2501 2606 2792 Size: 1173 28 3 99 9 3 105 186 Cuts at: 2792 1186 Size: 4077 Fragments arranged by size: 4077 1173 186 105 99 28 9 3 3 BseYI C'CCAG_C Cuts at: 2608 2727 2759 4886 5382 2608 Size: 119 32 2127 496 2909 Fragments arranged by size: 2909 2127 496 119 32 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2216 2771 1163 Size: 573 480 555 4075 Fragments arranged by size: 4075 573 555 480 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2603 3133 3894 4922 1540 Size: 275 186 602 530 761 1028 2301 Fragments arranged by size: 2301 1028 761 602 530 275 186 BsiHKAI G_wGCw'C Cuts at: 3744 3829 4900 3744 Size: 85 1071 4527 Fragments arranged by size: 4527 1071 85 BsmI GAATG_Cn' Cuts at: 2141 3194 3287 2141 Size: 1053 93 4537 Fragments arranged by size: 4537 1053 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2679 2784 2834 3002 3744 3829 4900 Size: 399 889 105 50 168 742 85 1071 Cuts at: 4900 1391 Size: 2174 Fragments arranged by size: 2174 1071 889 742 399 168 105 85 50 BspEI T'CCGG_A Cuts at: 5346 5346 Size: 5683 BspHI T'CATG_A Cuts at: 3572 5302 3572 Size: 1730 3953 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2190 2824 3038 844 Size: 1346 634 214 3489 Fragments arranged by size: 3489 1346 634 214 BsrBI CCG'CTC Cuts at: 1394 1823 3570 1394 Size: 429 1747 3507 Fragments arranged by size: 3507 1747 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2754 4178 4352 65 Size: 1183 1506 1424 174 1396 Fragments arranged by size: 1506 1424 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4327 4514 1700 Size: 110 2517 187 2869 Fragments arranged by size: 2869 2517 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5683 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5683 BssSI C'ACGA_G Cuts at: 3743 4755 3743 Size: 1012 4671 BstAPI GCAn_nnn'nTGC Cuts at: 794 2629 794 Size: 1835 3848 BstBI TT'CG_AA Cuts at: 4487 4487 Size: 5683 BstEII G'GTnAC_C Cuts at: 1184 2803 1184 Size: 1619 4064 BstXI CCAn_nnnn'nTGG Cuts at: 2974 2974 Size: 5683 BstYI r'GATC_y Cuts at: 1972 3013 3380 3765 3782 5234 5320 5332 5678 Size: 1041 367 385 17 1452 86 12 346 Cuts at: 5678 1972 Size: 1977 Fragments arranged by size: 1977 1452 1041 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2331 513 Size: 552 1266 3865 Fragments arranged by size: 3865 1266 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4944 Fragments arranged by size: 4944 683 56 BtsI GCAGTG_nn' Cuts at: 2143 2192 2641 3199 3993 4013 2143 Size: 49 449 558 794 20 3813 Fragments arranged by size: 3813 794 558 449 49 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5683 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5648 DraI TTT'AAA Cuts at: 2987 2987 Size: 5683 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4690 809 Size: 507 3374 1802 Fragments arranged by size: 3374 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2098 3130 4019 Size: 54 1425 50 275 18 268 1032 889 Cuts at: 4019 5648 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1032 889 275 268 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3130 1537 Size: 275 1318 4090 Fragments arranged by size: 4090 1318 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2364 2679 3613 1353 Size: 1011 315 934 3423 Fragments arranged by size: 3423 1011 934 315 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2480 4270 4644 4790 387 Size: 1064 138 891 1790 374 146 1280 Fragments arranged by size: 1790 1280 1064 891 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2068 2388 2501 2564 2888 3746 4343 Size: 393 436 320 113 63 324 858 597 Cuts at: 4343 5130 5398 1239 Size: 787 268 1524 Fragments arranged by size: 1524 858 787 597 436 393 324 320 268 113 63 EcoNI CCTnn'n_nnAGG Cuts at: 2130 2504 2684 2870 2130 Size: 374 180 186 4943 Fragments arranged by size: 4943 374 186 180 EcoO109I rG'GnC_Cy Cuts at: 2179 2179 Size: 5683 EcoRI G'AATT_C Cuts at: 2992 3370 2992 Size: 378 5305 FspI TGC'GCA Cuts at: 2067 2633 4189 2067 Size: 566 1556 3561 Fragments arranged by size: 3561 1556 566 HaeII r_GCGC'y Cuts at: 1678 2599 2658 2734 4830 1678 Size: 921 59 76 2096 2531 Fragments arranged by size: 2531 2096 921 76 59 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2024 2056 2345 2377 2477 Size: 32 135 32 452 32 289 32 100 Cuts at: 2477 2509 2585 2617 2681 2713 2861 2893 2930 Size: 32 76 32 64 32 148 32 37 Cuts at: 2930 2962 4324 4356 4398 4430 1373 Size: 32 1362 32 42 32 2626 Fragments arranged by size: 2626 1362 452 289 148 135 100 76 64 42 37 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3027 3273 669 Size: 988 281 1089 246 3079 Fragments arranged by size: 3079 1089 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5683 HpaI GTT'AAC Cuts at: 3273 3273 Size: 5683 KpnI G_GTAC'C Cuts at: 3008 3008 Size: 5683 MfeI C'AATT_G Cuts at: 3282 3282 Size: 5683 MluI A'CGCG_T Cuts at: 5559 5559 Size: 5683 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2247 4797 4981 1305 Size: 942 2550 184 2007 Fragments arranged by size: 2550 2007 942 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3861 Fragments arranged by size: 3861 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2216 2330 2555 2801 3641 4000 4159 Size: 648 1050 114 225 246 840 359 159 Cuts at: 4159 518 Size: 2042 Fragments arranged by size: 2042 1050 840 648 359 246 225 159 114 MspA1I CmG'CkG Cuts at: 1606 2055 2088 2359 2934 3099 3776 4924 5169 Size: 449 33 271 575 165 677 1148 245 Cuts at: 5169 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 575 449 271 245 165 33 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5131 NdeI CA'TA_TG Cuts at: 387 387 Size: 5683 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5683 NotI GC'GGCC_GC Cuts at: 3130 3130 Size: 5683 NspI r_CATG'y Cuts at: 1132 1229 3041 4586 1132 Size: 97 1812 1545 2229 Fragments arranged by size: 2229 1812 1545 97 PasI CC'CwG_GG Cuts at: 1348 2615 1348 Size: 1267 4416 PciI A'CATG_T Cuts at: 4582 4582 Size: 5683 PflMI CCAn_nnn'nTGG Cuts at: 1108 2303 2615 1108 Size: 1195 312 4176 Fragments arranged by size: 4176 1195 312 PfoI T'CCnGG_A Cuts at: 2865 2865 Size: 5683 PmeI GTTT'AAAC Cuts at: 2987 2987 Size: 5683 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3752 3784 5265 5297 3752 Size: 32 1481 32 4138 Fragments arranged by size: 4138 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2179 2179 Size: 5683 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5683 PsiI TTA'TAA Cuts at: 3253 3253 Size: 5683 PstI C_TGCA'G Cuts at: 830 2187 2419 2463 2505 2526 2625 3035 830 Size: 1357 232 44 42 21 99 410 3478 Fragments arranged by size: 3478 1357 410 232 99 44 42 21 PvuI CG_AT'CG Cuts at: 2001 2603 2001 Size: 602 5081 PvuII CAG'CTG Cuts at: 2359 2359 Size: 5683 SalI G'TCGA_C Cuts at: 1936 3025 1936 Size: 1089 4594 SbfI CC_TGCA'GG Cuts at: 3035 3035 Size: 5683 ScaI AGT'ACT Cuts at: 3931 5643 3931 Size: 1712 3971 SfcI C'TryA_G Cuts at: 826 1045 2183 2248 2415 2459 2501 2522 2621 Size: 219 1138 65 167 44 42 21 99 Cuts at: 2621 2658 3031 4166 4847 5038 826 Size: 37 373 1135 681 191 1471 Fragments arranged by size: 1471 1138 1135 681 373 219 191 167 99 65 44 42 37 21 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5683 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5683 SmaI CCC'GGG Cuts at: 1872 2023 2045 3010 1872 Size: 151 22 965 4545 Fragments arranged by size: 4545 965 151 22 SmlI C'TyrA_G Cuts at: 820 1017 1943 3787 4688 4950 820 Size: 197 926 1844 901 262 1553 Fragments arranged by size: 1844 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3474 493 Size: 2981 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5683 SphI G_CATG'C Cuts at: 1132 3041 1132 Size: 1909 3774 SspI AAT'ATT Cuts at: 5 52 3607 5 Size: 47 3555 2081 Fragments arranged by size: 3555 2081 47 StuI AGG'CCT Cuts at: 1517 2499 1517 Size: 982 4701 StyI C'CwwG_G Cuts at: 513 1065 2907 3122 513 Size: 552 1842 215 3074 Fragments arranged by size: 3074 1842 552 215 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3710 3727 3880 4065 4510 325 Size: 1255 2130 17 153 185 445 1498 Fragments arranged by size: 2130 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3929 5641 96 Size: 274 80 33 51 1443 1952 1712 138 Fragments arranged by size: 1952 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2060 2092 2171 2203 5643 5675 2060 Size: 32 79 32 3440 32 2068 Fragments arranged by size: 3440 2068 79 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5683 XbaI T'CTAG_A Cuts at: 3019 3019 Size: 5683 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5683 XmaI C'CCGG_G Cuts at: 1870 2021 2043 3008 1870 Size: 151 22 965 4545 Fragments arranged by size: 4545 965 151 22 XmnI GAAnn'nnTTC Cuts at: 3812 3812 Size: 5683 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3491 276 Size: 53 83 186 936 1957 2468 Fragments arranged by size: 2468 1957 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI