Restriction map for
pFN21AB7990
(Linear) (Six-base) MAPSORT of: KIBB7990.seq Check: 5176 from: 1 to: 978
KIBB7990.orf
With 182 enzymes: SgfI *
October 7, 2009 14:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 819 978
Size: 819 159
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 383 978
Size: 383 595
AfeI AGC'GCT
Cuts at: 0 727 978
Size: 727 251
AhdI GACnn_n'nnGTC
Cuts at: 0 33 978
Size: 33 945
AleI CACnn'nnGTG
Cuts at: 0 325 978
Size: 325 653
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 435 978
Size: 435 543
AlwNI CAG_nnn'CTG
Cuts at: 0 177 610 978
Size: 177 433 368
Fragments arranged by size:
433 368 177
ApoI r'AATT_y
Cuts at: 0 103 978
Size: 103 875
AvaI C'yCGr_G
Cuts at: 0 16 38 583 978
Size: 16 22 545 395
Fragments arranged by size:
545 395 22 16
BanI G'GyrC_C
Cuts at: 0 319 826 978
Size: 319 507 152
Fragments arranged by size:
507 319 152
BanII G_rGCy'C
Cuts at: 0 674 779 978
Size: 674 105 199
Fragments arranged by size:
674 199 105
BbsI GAAGACnn'nnnn_
Cuts at: 0 834 978
Size: 834 144
BclI T'GATC_A
Cuts at: 0 203 305 978
Size: 203 102 673
Fragments arranged by size:
673 203 102
BlpI GC'TnA_GC
Cuts at: 0 512 978
Size: 512 466
Bme1580I G_kGCm'C
Cuts at: 0 829 978
Size: 829 149
BmrI ACTGGGnnnn_n'
Cuts at: 0 559 978
Size: 559 419
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 93 978
Size: 93 885
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 63 496 559 883 978
Size: 63 433 63 324 95
Fragments arranged by size:
433 324 95 63 63
Bpu10I CC'TnA_GC
Cuts at: 0 126 978
Size: 126 852
BsaHI Gr'CG_yC
Cuts at: 0 432 978
Size: 432 546
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 472 502 532 562 856 886 978
Size: 472 30 30 30 294 30 92
Fragments arranged by size:
472 294 92 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 354 382 385 484 493 496 601 787
Size: 354 28 3 99 9 3 105 186
Cuts at: 787 978
Size: 191
Fragments arranged by size:
354 191 186 105 99 28 9 3
3
BseYI C'CCAG_C
Cuts at: 0 603 722 754 978
Size: 603 119 32 224
Fragments arranged by size:
603 224 119 32
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 211 766 978
Size: 211 555 212
Fragments arranged by size:
555 212 211
BsiEI CG_ry'CG
Cuts at: 0 598 978
Size: 598 380
BsmI GAATG_Cn'
Cuts at: 0 136 978
Size: 136 842
Bsp1286I G_dGCh'C
Cuts at: 0 674 779 829 978
Size: 674 105 50 149
Fragments arranged by size:
674 149 105 50
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 185 819 978
Size: 185 634 159
Fragments arranged by size:
634 185 159
BsrDI GCAATG_nn'
Cuts at: 0 749 978
Size: 749 229
BstAPI GCAn_nnn'nTGC
Cuts at: 0 624 978
Size: 624 354
BstEII G'GTnAC_C
Cuts at: 0 798 978
Size: 798 180
BstXI CCAn_nnnn'nTGG
Cuts at: 0 969 978
Size: 969 9
BtgI C'CryG_G
Cuts at: 0 326 978
Size: 326 652
BtsI GCAGTG_nn'
Cuts at: 0 138 187 636 978
Size: 138 49 449 342
Fragments arranged by size:
449 342 138 49
EaeI y'GGCC_r
Cuts at: 0 93 978
Size: 93 885
EarI CTCTTCn'nnn_
Cuts at: 0 359 674 978
Size: 359 315 304
Fragments arranged by size:
359 315 304
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 475 978
Size: 475 503
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 63 383 496 559 883 978
Size: 63 320 113 63 324 95
Fragments arranged by size:
324 320 113 95 63 63
EcoNI CCTnn'n_nnAGG
Cuts at: 0 125 499 679 865 978
Size: 125 374 180 186 113
Fragments arranged by size:
374 186 180 125 113
EcoO109I rG'GnC_Cy
Cuts at: 0 174 978
Size: 174 804
FspI TGC'GCA
Cuts at: 0 62 628 978
Size: 62 566 350
Fragments arranged by size:
566 350 62
HaeII r_GCGC'y
Cuts at: 0 594 653 729 978
Size: 594 59 76 249
Fragments arranged by size:
594 249 76 59
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 19 51 340 372 472 504 580 612
Size: 19 32 289 32 100 32 76 32
Cuts at: 612 676 708 856 888 925 957 978
Size: 64 32 148 32 37 32 21
Fragments arranged by size:
289 148 100 76 64 37 32 32
32 32 32 32 32 21 19
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 242 978
Size: 242 736
MslI CAynn'nnrTG
Cuts at: 0 211 325 550 796 978
Size: 211 114 225 246 182
Fragments arranged by size:
246 225 211 182 114
MspA1I CmG'CkG
Cuts at: 0 50 83 354 929 978
Size: 50 33 271 575 49
Fragments arranged by size:
575 271 50 49 33
PasI CC'CwG_GG
Cuts at: 0 610 978
Size: 610 368
PflMI CCAn_nnn'nTGG
Cuts at: 0 298 610 978
Size: 298 312 368
Fragments arranged by size:
368 312 298
PfoI T'CCnGG_A
Cuts at: 0 860 978
Size: 860 118
PpuMI rG'GwC_Cy
Cuts at: 0 174 978
Size: 174 804
PstI C_TGCA'G
Cuts at: 0 182 414 458 500 521 620 978
Size: 182 232 44 42 21 99 358
Fragments arranged by size:
358 232 182 99 44 42 21
PvuI CG_AT'CG
Cuts at: 0 598 978
Size: 598 380
PvuII CAG'CTG
Cuts at: 0 354 978
Size: 354 624
SfcI C'TryA_G
Cuts at: 0 178 243 410 454 496 517 616 653
Size: 178 65 167 44 42 21 99 37
Cuts at: 653 978
Size: 325
Fragments arranged by size:
325 178 167 99 65 44 42 37
21
SmaI CCC'GGG
Cuts at: 0 18 40 978
Size: 18 22 938
Fragments arranged by size:
938 22 18
StuI AGG'CCT
Cuts at: 0 494 978
Size: 494 484
StyI C'CwwG_G
Cuts at: 0 902 978
Size: 902 76
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 55 87 166 198 978
Size: 55 32 79 32 780
Fragments arranged by size:
780 79 55 32 32
XmaI C'CCGG_G
Cuts at: 0 16 38 978
Size: 16 22 940
Fragments arranged by size:
940 22 16
Enzymes that do cut:
AarI AcuI AfeI AhdI AleI AlfI AlwNI ApoI
AvaI BanI BanII BbsI BclI BlpI Bme1580I BmrI
BplI BpmI Bpu10I BsaHI BsaXI BseRI BseYI BsgI
BsiEI BsmI Bsp1286I BspMI BsrDI BstAPI BstEII BstXI
BtgI BtsI EaeI EarI EciI Eco57MI EcoNI EcoO109I
FspI HaeII Hin4I MmeI MslI MspA1I PasI PflMI
PfoI PpuMI PstI PvuI PvuII SfcI SmaI StuI
StyI TstI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AflIII AgeI AloI
ApaI ApaLI AscI AseI AsiSI AvrII BaeI BamHI
BbeI BbvCI BcgI BciVI BfrBI BglI BglII BmgBI
BmtI BpuEI BsaI BsaAI BsaBI BsaWI BsiHKAI BsiWI
BsmBI BspEI BspHI BsrBI BsrFI BsrGI BssHII BssSI
BstBI BstYI BstZ17I Bsu36I BtgZI ClaI CspCI DraI
DraIII DrdI EagI EcoICRI EcoRI EcoRV FalI FseI
FspAI HincII HindIII HpaI KasI KpnI MfeI MluI
MscI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PciI PmeI PmlI PpiI
PshAI PsiI PspOMI PspXI PsrI RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI
SwaI TaqII TatI Tth111I XbaI XcmI XhoI XmnI
ZraI