################################## # NO CUT ENZYMES ( 199 enzymes) # ################################## AatII AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I AspEI AspHI AspI AvaI AviII AvrII BamHI BanII BbeI BbrPI BbsI BbvCI BbvI BcgI BclI BcnI BfrI BglI BglII BlnI BlpI BmyI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsoBI Bsp1286I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrI BssHII BssKI BssSI Bst1107I BstAPI BstBI BstEII BstNI BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DdeI DraI DraII DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRII EcoRV EcoT22I FauI FbaI Fnu4HI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII ItaI KasI KspI MaeII MamI MfeI MflI MluI MroI MseI MspA1I MspI MunI MvaI MvnI MwoI NaeI NarI NciI NcoI NdeI NgoMIV NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI ScrFI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI StuI SwaI TaqI Tru9I TseI Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 34 enzymes) # ################################### Acc65I 85 GGTACC G^GTACC AciI 306 CCGC CCGC(-3/-1) AcsI 250 AAATTC [GA]^AATT[CT] ApoI 250 AAATTC [GA]^AATT[CT] Asp718I 85 GGTACC G^GTACC AvaII 98 GGACC G^G[AT]CC BanI 85 GGTACC G^G[CT][GA]CC BciVI 58 GGATAC GTATCC(6/5) BfaI 182 CTAG C^TAG BpmI 239 CTCCAG CTGGAG(16/14) BsmI 244 GAATGC GAATGC(1/-1) Bsp1407I 434 TGTACA T^GTACA BsrGI 434 TGTACA T^GTACA BstF5I 165 CATCC GGATG(2/0) DpnII 285 GATC ^GATC DsaI 143 CCGTGG C^C[GA][CT]GG EarI 127 CTCTTC CTCTTC(1/4) FokI 165 CATCC GGATG(9/13) HphI 205 GGTGA GGTGA(8/7) KpnI 85 GGTACC GGTAC^C Ksp632I 127 CTCTTC CTCTTC(1/4) MaeI 182 CTAG C^TAG MaeIII 34 GTGAC ^GT[ACGT]AC MboI 285 GATC ^GATC MslI 76 CACCCAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NdeII 285 GATC ^GATC NheI 181 GCTAGC G^CTAGC PleI 341 GACTC GAGTC(4/5) PstI 67 CTGCAG CTGCA^G Sau3AI 285 GATC ^GATC SfaNI 72 GATGC GCATC(5/9) SspBI 434 TGTACA T^GTACA Tsp45I 34 GTGAC ^GT[GC]AC TspRI 299 CACTG CA[GC]TG[ACGT][ACGT]^ ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### AluI 20 AGCT AG^CT 327 AGCT BalI 161 TGGCCA TGG^CCA 194 TGGCCA BsiYI 89 CCATTGATGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 186 CCTCTATGTGG BslI 89 CCATTGATGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 186 CCTCTATGTGG BsmFI 97 GGGAC GGGAC(10/14) 339 GGGAC Cfr13I 98 GGACC G^G[ACGT]CC 147 GGGCC EaeI 161 TGGCCA [CT]^GGCC[GA] 194 TGGCCA EcoT14I 101 CCAAGG C^C[AT][AT]GG 168 CCAAGG MluNI 161 TGGCCA TGG^CCA 194 TGGCCA MscI 161 TGGCCA TGG^CCA 194 TGGCCA NlaIV 85 GGTACC GG[ACGT]^[ACGT]CC 97 GGGACC Sau96I 98 GGACC G^G[ACGT]CC 147 GGGCC SfcI 67 CTGCAG C^T[GA][CT]AG 219 CTACAG StyI 101 CCAAGG C^C[AT][AT]GG 168 CCAAGG ######################################### # 3 OR MORE CUT ENZYMES ( 11 enzymes) # ######################################### AfaI BsaJI Cac8I HaeIII HinfI MboII MnlI NlaIII RsaI TfiI Tsp509I