################################## # NO CUT ENZYMES ( 111 enzymes) # ################################## AatII AccIII AclI AflII AgeI AhdI AocI AscI AseI AsnI Asp700I AspEI AspI AvaI AviII AvrII BbeI BbvCI BcnI BfrI BlnI BlpI Bpu1102I BseAI BsgI BsiEI BsiWI BsoBI Bsp1407I BspDI BspEI BspHI BsrDI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco52I Eco81I EcoNI EcoO65I EcoRV EcoT22I FseI FspI HincII HindII HindIII HpaI KasI MfeI MroI MunI NarI NciI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmeI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tth111I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### Acc65I 520 GGTACC G^GTACC AccI 379 GTCTAC GT^[AC][GT]AC AcsI 782 GAATTC [GA]^AATT[CT] AcyI 298 GGCGTC G[GA]^CG[CT]C Alw44I 1509 GTGCAC G^TGCAC ApaLI 1509 GTGCAC G^TGCAC ApoI 782 GAATTC [GA]^AATT[CT] Asp718I 520 GGTACC G^GTACC AspHI 1509 GTGCAC G[AT]GC[AT]^C BalI 1376 TGGCCA TGG^CCA BbrPI 1222 CACGTG CAC^GTG BcgI 1738 GCAGGACCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 51 GTATCC GTATCC(6/5) BclI 1295 TGATCA T^GATCA BglI 290 GCCAGGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaBI 360 GATCGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 298 GGCGTC G[GA]^CG[CT]C BsaI 12 GGTCTC GGTCTC(1/5) BsaWI 1241 ACCGGA [AT]^CCGG[AT] BsiHKAI 1509 GTGCAC G[AT]GC[AT]^C BsmBI 1275 CGTCTC CGTCTC(1/5) BspLU11I 1211 ACATGT A^CATGT BspMI 440 ACCTGC ACCTGC(4/8) BsrFI 982 GCCGGC [GA]^CCGG[CT] BstAPI 1026 GCAGGGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 971 CCAGCTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr10I 982 GCCGGC [GA]^CCGG[CT] EcoRI 782 GAATTC G^AATTC FbaI 1295 TGATCA T^GATCA Hin1I 298 GGCGTC G[GA]^CG[CT]C KpnI 520 GGTACC GGTAC^C KspI 1087 CCGCGG CCGC^GG MamI 360 GATCGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluI 811 ACGCGT A^CGCGT MluNI 1376 TGGCCA TGG^CCA MscI 1376 TGGCCA TGG^CCA MseI 745 TTAA T^TAA NaeI 982 GCCGGC GCC^GGC NgoMIV 982 GCCGGC G^CCGGC PflMI 594 CCAGCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 1222 CACGTG CAC^GTG PmlI 1222 CACGTG CAC^GTG PvuII 1502 CAGCTG CAG^CTG SacII 1087 CCGCGG CCGC^GG SapI 1488 GCTCTTC GCTCTTC(1/4) SmlI 1666 CTCAAG C^T[CT][GA]AG TfiI 1688 GAATC G^A[AT]TC Tru9I 745 TTAA T^TAA Van91I 594 CCAGCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AccII 812 CGCG CG^CG 1088 CGCG Aor51HI 1200 AGCGCT AGC^GCT 1421 AGCGCT BamHI 590 GGATCC G^GATCC 822 GGATCC BglII 317 AGATCT A^GATCT 578 AGATCT BseRI 370 CTCCTC GAGGAG(10/8) 1115 CTCCTC BsrBI 91 GAGCGG CCGCTC(-3/-3) 562 GAGCGG BstUI 812 CGCG CG^CG 1088 CGCG Eco47III 1200 AGCGCT AGC^GCT 1421 AGCGCT HaeII 1200 AGCGCT [GA]GCGC^[CT] 1421 AGCGCT HapII 983 CCGG C^CGG 1242 CCGG HgaI 299 GCGTC GACGC(5/10) 813 GCGTC HpaII 983 CCGG C^CGG 1242 CCGG MslI 387 CATCATTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 729 CATGCTGGTG MspI 983 CCGG C^CGG 1242 CCGG MvnI 812 CGCG CG^CG 1088 CGCG NcoI 1462 CCATGG C^CATGG 1622 CCATGG NspI 1211 ACATGT [GA]CATG^[CT] 1358 GCATGT PpuMI 1740 AGGACCC [GA]G^G[AT]CC[CT] 1765 AGGACCT SfcI 930 CTACAG C^T[GA][CT]AG 1650 CTACAG Tsp509I 747 AATT ^AATT 783 AATT XcmI 686 CCACAGGCAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1037 CCATTTCTGAGGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 77 enzymes) # ######################################### AciI AfaI AflIII AluI AlwI AlwNI ApaI AvaII BanI BanII BbsI BbvI BfaI BmyI BpmI BpuAI BsaAI BsaJI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HhaI HinP1I HinfI HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MspA1I MvaI MwoI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SfaNI StyI TaqI TseI Tsp45I TspRI TthHB8I XhoII