(Circular) (Six-base) MAPSORT of: pFN21AE6412.seq Check: 9695 from: 1 to: 6511 pFN21AE6412 6511 bp With 182 enzymes: SgfI * March 18, 2015 14:51 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4321 278 Size: 53 83 186 936 2785 2468 Fragments arranged by size: 2785 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2385 3854 1937 Size: 448 1469 4594 Fragments arranged by size: 4594 1469 448 Acc65I G'GTAC_C Cuts at: 2525 3832 2525 Size: 1307 5204 AclI AA'CG_TT Cuts at: 1563 4638 5011 1563 Size: 3075 373 3063 Fragments arranged by size: 3075 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2410 3667 4574 5958 6226 1632 Size: 778 1257 907 1384 268 1917 Fragments arranged by size: 1917 1384 1257 907 778 268 AfeI AGC'GCT Cuts at: 3207 3428 3207 Size: 221 6290 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6314 AflIII A'CryG_T Cuts at: 1203 2816 3216 3228 3327 3576 5410 6387 1203 Size: 1613 400 12 99 249 1834 977 1327 Fragments arranged by size: 1834 1613 1327 977 400 249 99 12 AgeI A'CCGG_T Cuts at: 5342 5342 Size: 6511 AhdI GACnn_n'nnGTC Cuts at: 5240 5240 Size: 6511 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6511 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6511 AlwNI CAG_nnn'CTG Cuts at: 2141 2605 2943 3068 4254 5826 2141 Size: 464 338 125 1186 1572 2826 Fragments arranged by size: 2826 1572 1186 464 338 125 ApaI G_GGCC'C Cuts at: 2413 2614 3680 2413 Size: 201 1066 5244 Fragments arranged by size: 5244 1066 201 ApaLI G'TGCA_C Cuts at: 3514 4568 5724 3514 Size: 1054 1156 4301 Fragments arranged by size: 4301 1156 1054 ApoI r'AATT_y Cuts at: 1493 2787 3820 4046 4198 6307 1493 Size: 1294 1033 226 152 2109 1697 Fragments arranged by size: 2109 1697 1294 1033 226 152 AseI AT'TA_AT Cuts at: 160 1032 5065 160 Size: 872 4033 1606 Fragments arranged by size: 4033 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6511 AvaI C'yCGr_G Cuts at: 1870 1943 3836 1870 Size: 73 1893 4545 Fragments arranged by size: 4545 1893 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2039 2072 2039 Size: 33 6478 BamHI G'GATC_C Cuts at: 2595 2827 3841 4208 2595 Size: 232 1014 367 4898 Fragments arranged by size: 4898 1014 367 232 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2087 2207 2525 2852 2889 Size: 325 217 627 300 120 318 327 37 Cuts at: 2889 2972 3037 3832 5287 618 Size: 83 65 795 1455 1842 Fragments arranged by size: 1842 1455 795 627 327 325 318 300 217 120 83 65 37 BanII G_rGCy'C Cuts at: 1391 2413 2614 3680 3830 1391 Size: 1022 201 1066 150 4072 Fragments arranged by size: 4072 1066 1022 201 150 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2224 2251 2510 3453 928 Size: 971 325 27 259 943 3986 Fragments arranged by size: 3986 971 943 325 259 27 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3732 3766 4702 4736 1903 Size: 34 1795 34 936 34 3678 Fragments arranged by size: 3678 1795 936 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2067 4403 5613 2067 Size: 2336 1210 2965 Fragments arranged by size: 2965 2336 1210 BclI T'GATC_A Cuts at: 1548 3300 1548 Size: 1752 4759 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2301 5122 136 Size: 107 122 71 631 605 629 2821 1525 Fragments arranged by size: 2821 1525 631 629 605 122 107 71 BglII A'GATC_T Cuts at: 2322 2583 6506 2322 Size: 261 3923 2327 Fragments arranged by size: 3923 2327 261 BlpI GC'TnA_GC Cuts at: 3928 3928 Size: 6511 Bme1580I G_kGCm'C Cuts at: 1790 2106 2413 2614 3042 3518 3680 3713 4572 Size: 316 307 201 428 476 162 33 859 Cuts at: 4572 5728 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 859 476 428 316 307 201 162 33 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6511 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4242 5200 442 Size: 416 53 83 399 24 2825 958 1753 Fragments arranged by size: 2825 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6511 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2114 2191 2288 2335 3667 5171 1239 Size: 875 77 97 47 1332 1504 2579 Fragments arranged by size: 2579 1504 1332 875 97 77 47 Bpu10I CC'TnA_GC Cuts at: 2158 2158 Size: 6511 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3656 4636 5501 5799 3656 Size: 980 865 298 4368 Fragments arranged by size: 4368 980 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2023 5174 882 Size: 617 524 3151 2219 Fragments arranged by size: 3151 2219 617 524 BsaAI yAC'GTr Cuts at: 493 1204 3229 3328 3579 4302 493 Size: 711 2025 99 251 723 2702 Fragments arranged by size: 2702 2025 723 711 251 99 BsaBI GATnn'nnATC Cuts at: 2369 6505 2369 Size: 4136 2375 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2304 4318 4700 5335 Size: 53 83 186 936 771 2014 382 635 Cuts at: 5335 275 Size: 1451 Fragments arranged by size: 2014 1451 936 771 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3246 4944 5342 5616 5763 6174 3246 Size: 1698 398 274 147 411 3583 Fragments arranged by size: 3583 1698 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3261 3291 1178 Size: 30 165 30 1858 30 4398 Fragments arranged by size: 4398 1858 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2366 3111 1186 Size: 1180 745 4586 Fragments arranged by size: 4586 1180 745 BseYI C'CCAG_C Cuts at: 2141 2619 3712 3729 3739 5714 6210 2141 Size: 478 1093 17 10 1975 496 2442 Fragments arranged by size: 2442 1975 1093 496 478 17 10 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5938 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3961 4722 5750 1540 Size: 275 186 1960 761 1028 2301 Fragments arranged by size: 2301 1960 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3518 4572 4657 5728 3518 Size: 1054 85 1071 4301 Fragments arranged by size: 4301 1071 1054 85 BsmI GAATG_Cn' Cuts at: 2250 2542 3636 4022 4115 2250 Size: 292 1094 386 93 4646 Fragments arranged by size: 4646 1094 386 292 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3286 3286 Size: 6511 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2106 2413 2614 3042 3518 3680 3713 Size: 399 316 307 201 428 476 162 33 Cuts at: 3713 3830 4572 4657 5728 1391 Size: 117 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 476 428 399 316 307 201 162 117 85 33 BspEI T'CCGG_A Cuts at: 6174 6174 Size: 6511 BspHI T'CATG_A Cuts at: 4400 6130 4400 Size: 1730 4781 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2454 3866 844 Size: 1610 1412 3489 Fragments arranged by size: 3489 1610 1412 BsrBI CCG'CTC Cuts at: 1394 1823 2098 2569 4398 1394 Size: 429 275 471 1829 3507 Fragments arranged by size: 3507 1829 471 429 275 BsrDI GCAATG_nn' Cuts at: 65 1248 5006 5180 65 Size: 1183 3758 174 1396 Fragments arranged by size: 3758 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2987 5155 5342 1700 Size: 110 1177 2168 187 2869 Fragments arranged by size: 2869 2168 1177 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6511 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6511 BssSI C'ACGA_G Cuts at: 4571 5583 4571 Size: 1012 5499 BstAPI GCAn_nnn'nTGC Cuts at: 794 3037 794 Size: 2243 4268 BstBI TT'CG_AA Cuts at: 5315 5315 Size: 6511 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6511 BstXI CCAn_nnnn'nTGG Cuts at: 2983 2983 Size: 6511 BstYI r'GATC_y Cuts at: 1972 2322 2583 2595 2827 3841 4208 4593 4610 Size: 350 261 12 232 1014 367 385 17 Cuts at: 4610 6062 6148 6160 6506 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1014 385 367 350 346 261 232 86 17 12 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3092 3467 3584 3627 513 Size: 552 939 1088 375 117 43 3397 Fragments arranged by size: 3397 1088 939 552 375 117 43 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2932 3067 3609 487 Size: 683 56 1706 135 542 3389 Fragments arranged by size: 3389 1706 683 542 135 56 BtsI GCAGTG_nn' Cuts at: 3776 3805 4027 4821 4841 3776 Size: 29 222 794 20 5446 Fragments arranged by size: 5446 794 222 29 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6511 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6476 DraI TTT'AAA Cuts at: 3815 3815 Size: 6511 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5518 809 Size: 507 4202 1802 Fragments arranged by size: 4202 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2715 2985 3054 Size: 54 1425 50 275 18 885 270 69 Cuts at: 3054 3354 3381 3958 4847 6476 8 Size: 300 27 577 889 1629 43 Fragments arranged by size: 1629 1425 889 885 577 300 275 270 69 54 50 43 27 18 EagI C'GGCC_G Cuts at: 1537 1812 3958 1537 Size: 275 2146 4090 Fragments arranged by size: 4090 2146 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2429 3500 3536 4441 1353 Size: 1076 1071 36 905 3423 Fragments arranged by size: 3423 1076 1071 905 36 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3333 5098 5472 5618 387 Size: 1064 138 1744 1765 374 146 1280 Fragments arranged by size: 1765 1744 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2114 2191 2288 2335 2410 3667 4574 Size: 393 482 77 97 47 75 1257 907 Cuts at: 4574 5171 5958 6226 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1257 907 787 597 482 393 268 97 77 75 47 EcoO109I rG'GnC_Cy Cuts at: 2410 2610 2611 3065 3676 3677 3746 3771 2410 Size: 200 1 454 611 1 69 25 5150 Fragments arranged by size: 5150 611 454 200 69 25 1 1 EcoRI G'AATT_C Cuts at: 2787 3820 4198 2787 Size: 1033 378 5100 Fragments arranged by size: 5100 1033 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3697 3697 Size: 6511 FspI TGC'GCA Cuts at: 5017 5017 Size: 6511 HaeII r_GCGC'y Cuts at: 1678 3209 3430 5658 1678 Size: 1531 221 2228 2531 Fragments arranged by size: 2531 2228 1531 221 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3261 3293 3678 3682 3710 Size: 32 135 32 1689 32 385 4 28 Cuts at: 3710 3714 3785 3817 5152 5184 5226 5258 1373 Size: 4 71 32 1335 32 42 32 2626 Fragments arranged by size: 2626 1689 1335 385 135 71 42 32 32 32 32 32 32 28 4 4 HincII GTy'rAC Cuts at: 669 1657 1938 3855 4101 669 Size: 988 281 1917 246 3079 Fragments arranged by size: 3079 1917 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6511 HpaI GTT'AAC Cuts at: 4101 4101 Size: 6511 KpnI G_GTAC'C Cuts at: 2529 3836 2529 Size: 1307 5204 MfeI C'AATT_G Cuts at: 4110 4110 Size: 6511 MluI A'CGCG_T Cuts at: 2816 6387 2816 Size: 3571 2940 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5625 5809 1305 Size: 4320 184 2007 Fragments arranged by size: 4320 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3383 10 Size: 54 1425 343 1551 3138 Fragments arranged by size: 3138 1551 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2396 2738 4469 4828 4987 518 Size: 648 1230 342 1731 359 159 2042 Fragments arranged by size: 2042 1731 1230 648 359 342 159 MspA1I CmG'CkG Cuts at: 1606 2970 3094 3509 3556 3733 3927 4604 5752 Size: 1364 124 415 47 177 194 677 1148 Cuts at: 5752 5997 1606 Size: 245 2120 Fragments arranged by size: 2120 1364 1148 677 415 245 194 177 124 47 NaeI GCC'GGC Cuts at: 2989 2989 Size: 6511 NcoI C'CATG_G Cuts at: 513 1065 2004 3467 3627 513 Size: 552 939 1463 160 3397 Fragments arranged by size: 3397 1463 939 552 160 NdeI CA'TA_TG Cuts at: 387 387 Size: 6511 NgoMIV G'CCGG_C Cuts at: 2987 2987 Size: 6511 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6511 NotI GC'GGCC_GC Cuts at: 3958 3958 Size: 6511 NspI r_CATG'y Cuts at: 1132 1229 3220 3367 3869 5414 1132 Size: 97 1991 147 502 1545 2229 Fragments arranged by size: 2229 1991 1545 502 147 97 PasI CC'CwG_GG Cuts at: 1348 2451 2902 3601 1348 Size: 1103 451 699 4258 Fragments arranged by size: 4258 1103 699 451 PciI A'CATG_T Cuts at: 3216 5410 3216 Size: 2194 4317 PflMI CCAn_nnn'nTGG Cuts at: 1108 2605 1108 Size: 1497 5014 PmeI GTTT'AAAC Cuts at: 3815 3815 Size: 6511 PmlI CAC'GTG Cuts at: 3229 3229 Size: 6511 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4580 4612 6093 6125 4580 Size: 32 1481 32 4966 Fragments arranged by size: 4966 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3746 3771 3746 Size: 25 6486 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6511 PsiI TTA'TAA Cuts at: 4081 4081 Size: 6511 PspOMI G'GGCC_C Cuts at: 2409 2610 3676 2409 Size: 201 1066 5244 Fragments arranged by size: 5244 1066 201 PstI C_TGCA'G Cuts at: 830 3863 830 Size: 3033 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6511 PvuII CAG'CTG Cuts at: 3509 3509 Size: 6511 SacII CC_GC'GG Cuts at: 3095 3095 Size: 6511 SalI G'TCGA_C Cuts at: 1936 3853 1936 Size: 1917 4594 SapI GCTCTTCn'nnn_ Cuts at: 3500 3500 Size: 6511 SbfI CC_TGCA'GG Cuts at: 3863 3863 Size: 6511 ScaI AGT'ACT Cuts at: 4759 6471 4759 Size: 1712 4799 SfcI C'TryA_G Cuts at: 826 1045 2935 3655 3859 4994 5675 5866 826 Size: 219 1890 720 204 1135 681 191 1471 Fragments arranged by size: 1890 1471 1135 720 681 219 204 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6511 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6511 SmaI CCC'GGG Cuts at: 1872 3838 1872 Size: 1966 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3671 4615 5516 5778 820 Size: 197 926 1728 944 901 262 1553 Fragments arranged by size: 1728 1553 944 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4302 493 Size: 3809 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6511 SphI G_CATG'C Cuts at: 1132 3869 1132 Size: 2737 3774 SspI AAT'ATT Cuts at: 5 52 4435 5 Size: 47 4383 2081 Fragments arranged by size: 4383 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6511 StyI C'CwwG_G Cuts at: 513 1065 2004 2782 3265 3467 3627 3950 513 Size: 552 939 778 483 202 160 323 3074 Fragments arranged by size: 3074 939 778 552 483 323 202 160 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2870 2877 4538 4555 4708 4893 5338 Size: 1255 1290 7 1661 17 153 185 445 Cuts at: 5338 325 Size: 1498 Fragments arranged by size: 1661 1498 1290 1255 445 185 153 17 7 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2286 2706 3546 Size: 274 80 33 51 1443 309 420 840 Cuts at: 3546 3573 4757 6469 96 Size: 27 1184 1712 138 Fragments arranged by size: 1712 1443 1184 840 420 309 274 138 80 51 33 27 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3312 3344 6471 6503 3312 Size: 32 3127 32 3320 Fragments arranged by size: 3320 3127 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6511 XbaI T'CTAG_A Cuts at: 3847 3847 Size: 6511 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2698 3049 2698 Size: 351 6160 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6511 XmaI C'CCGG_G Cuts at: 1870 3836 1870 Size: 1966 4545 XmnI GAAnn'nnTTC Cuts at: 4640 4640 Size: 6511 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4319 276 Size: 53 83 186 936 2785 2468 Fragments arranged by size: 2785 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BbeI BbvCI BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NarI NruI NsiI PacI PfoI PspXI PsrI RsrII SacI SanDI SexAI SfiI SfoI SrfI SwaI