(Linear) (Six-base) MAPSORT of: KIEE6412.seq Check: 1984 from: 1 to: 1806 KIEE6412.orf With 182 enzymes: SgfI * March 16, 2015 11:31 .. AccI GT'mk_AC Cuts at: 0 380 1806 Size: 380 1426 Acc65I G'GTAC_C Cuts at: 0 520 1806 Size: 520 1286 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 405 1662 1806 Size: 405 1257 144 Fragments arranged by size: 1257 405 144 AfeI AGC'GCT Cuts at: 0 1202 1423 1806 Size: 1202 221 383 Fragments arranged by size: 1202 383 221 AflIII A'CryG_T Cuts at: 0 811 1211 1223 1322 1571 1806 Size: 811 400 12 99 249 235 Fragments arranged by size: 811 400 249 235 99 12 AlwNI CAG_nnn'CTG Cuts at: 0 136 600 938 1063 1806 Size: 136 464 338 125 743 Fragments arranged by size: 743 464 338 136 125 ApaI G_GGCC'C Cuts at: 0 408 609 1675 1806 Size: 408 201 1066 131 Fragments arranged by size: 1066 408 201 131 ApaLI G'TGCA_C Cuts at: 0 1509 1806 Size: 1509 297 ApoI r'AATT_y Cuts at: 0 782 1806 Size: 782 1024 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 34 67 1806 Size: 34 33 1739 Fragments arranged by size: 1739 34 33 BamHI G'GATC_C Cuts at: 0 590 822 1806 Size: 590 232 984 Fragments arranged by size: 984 590 232 BanI G'GyrC_C Cuts at: 0 82 202 520 847 884 967 1032 1806 Size: 82 120 318 327 37 83 65 774 Fragments arranged by size: 774 327 318 120 83 82 65 37 BanII G_rGCy'C Cuts at: 0 408 609 1675 1806 Size: 408 201 1066 131 Fragments arranged by size: 1066 408 201 131 BbsI GAAGACnn'nnnn_ Cuts at: 0 219 246 505 1448 1806 Size: 219 27 259 943 358 Fragments arranged by size: 943 358 259 219 27 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1727 1761 1806 Size: 1727 34 45 Fragments arranged by size: 1727 45 34 BciVI GTATCCnnnnn_n' Cuts at: 0 62 1806 Size: 62 1744 BclI T'GATC_A Cuts at: 0 1295 1806 Size: 1295 511 BglI GCCn_nnn'nGGC Cuts at: 0 296 1806 Size: 296 1510 BglII A'GATC_T Cuts at: 0 317 578 1806 Size: 317 261 1228 Fragments arranged by size: 1228 317 261 Bme1580I G_kGCm'C Cuts at: 0 101 408 609 1037 1513 1675 1708 1806 Size: 101 307 201 428 476 162 33 98 Fragments arranged by size: 476 428 307 201 162 101 98 33 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 109 186 283 330 1662 1806 Size: 109 77 97 47 1332 144 Fragments arranged by size: 1332 144 109 97 77 47 Bpu10I CC'TnA_GC Cuts at: 0 153 1806 Size: 153 1653 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1651 1806 Size: 1651 155 BsaI GGTCTCn'nnnn_ Cuts at: 0 18 1806 Size: 18 1788 BsaAI yAC'GTr Cuts at: 0 1224 1323 1574 1806 Size: 1224 99 251 232 Fragments arranged by size: 1224 251 232 99 BsaBI GATnn'nnATC Cuts at: 0 364 1806 Size: 364 1442 BsaHI Gr'CG_yC Cuts at: 0 299 1806 Size: 299 1507 BsaWI w'CCGG_w Cuts at: 0 1241 1806 Size: 1241 565 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1256 1286 1806 Size: 1256 30 520 Fragments arranged by size: 1256 520 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 361 1106 1806 Size: 361 745 700 Fragments arranged by size: 745 700 361 BseYI C'CCAG_C Cuts at: 0 136 614 1707 1724 1734 1806 Size: 136 478 1093 17 10 72 Fragments arranged by size: 1093 478 136 72 17 10 BsiHKAI G_wGCw'C Cuts at: 0 1513 1806 Size: 1513 293 BsmI GAATG_Cn' Cuts at: 0 245 537 1631 1806 Size: 245 292 1094 175 Fragments arranged by size: 1094 292 245 175 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1281 1806 Size: 1281 525 Bsp1286I G_dGCh'C Cuts at: 0 101 408 609 1037 1513 1675 1708 1806 Size: 101 307 201 428 476 162 33 98 Fragments arranged by size: 476 428 307 201 162 101 98 33 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 449 1806 Size: 449 1357 BsrBI CCG'CTC Cuts at: 0 93 564 1806 Size: 93 471 1242 Fragments arranged by size: 1242 471 93 BsrFI r'CCGG_y Cuts at: 0 982 1806 Size: 982 824 BstAPI GCAn_nnn'nTGC Cuts at: 0 1032 1806 Size: 1032 774 BstXI CCAn_nnnn'nTGG Cuts at: 0 978 1806 Size: 978 828 BstYI r'GATC_y Cuts at: 0 317 578 590 822 1806 Size: 317 261 12 232 984 Fragments arranged by size: 984 317 261 232 12 BtgI C'CryG_G Cuts at: 0 1087 1462 1579 1622 1806 Size: 1087 375 117 43 184 Fragments arranged by size: 1087 375 184 117 43 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 927 1062 1604 1806 Size: 927 135 542 202 Fragments arranged by size: 927 542 202 135 BtsI GCAGTG_nn' Cuts at: 0 1771 1800 1806 Size: 1771 29 6 Fragments arranged by size: 1771 29 6 EaeI y'GGCC_r Cuts at: 0 710 980 1049 1349 1376 1806 Size: 710 270 69 300 27 430 Fragments arranged by size: 710 430 300 270 69 27 EarI CTCTTCn'nnn_ Cuts at: 0 424 1495 1531 1806 Size: 424 1071 36 275 Fragments arranged by size: 1071 424 275 36 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1328 1806 Size: 1328 478 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 109 186 283 330 405 1662 1806 Size: 109 77 97 47 75 1257 144 Fragments arranged by size: 1257 144 109 97 77 75 47 EcoO109I rG'GnC_Cy Cuts at: 0 405 605 606 1060 1671 1672 1741 1766 Size: 405 200 1 454 611 1 69 25 Cuts at: 1766 1806 Size: 40 Fragments arranged by size: 611 454 405 200 69 40 25 1 1 EcoRI G'AATT_C Cuts at: 0 782 1806 Size: 782 1024 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1692 1806 Size: 1692 114 HaeII r_GCGC'y Cuts at: 0 1204 1425 1806 Size: 1204 221 381 Fragments arranged by size: 1204 381 221 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1256 1288 1673 1677 1705 1709 1780 1806 Size: 1256 32 385 4 28 4 71 26 Fragments arranged by size: 1256 385 71 32 28 26 4 4 KpnI G_GTAC'C Cuts at: 0 524 1806 Size: 524 1282 MluI A'CGCG_T Cuts at: 0 811 1806 Size: 811 995 MscI TGG'CCA Cuts at: 0 1378 1806 Size: 1378 428 MslI CAynn'nnrTG Cuts at: 0 391 733 1806 Size: 391 342 1073 Fragments arranged by size: 1073 391 342 MspA1I CmG'CkG Cuts at: 0 965 1089 1504 1551 1728 1806 Size: 965 124 415 47 177 78 Fragments arranged by size: 965 415 177 124 78 47 NaeI GCC'GGC Cuts at: 0 984 1806 Size: 984 822 NcoI C'CATG_G Cuts at: 0 1462 1622 1806 Size: 1462 160 184 Fragments arranged by size: 1462 184 160 NgoMIV G'CCGG_C Cuts at: 0 982 1806 Size: 982 824 NspI r_CATG'y Cuts at: 0 1215 1362 1806 Size: 1215 147 444 Fragments arranged by size: 1215 444 147 PasI CC'CwG_GG Cuts at: 0 446 897 1596 1806 Size: 446 451 699 210 Fragments arranged by size: 699 451 446 210 PciI A'CATG_T Cuts at: 0 1211 1806 Size: 1211 595 PflMI CCAn_nnn'nTGG Cuts at: 0 600 1806 Size: 600 1206 PmlI CAC'GTG Cuts at: 0 1224 1806 Size: 1224 582 PpuMI rG'GwC_Cy Cuts at: 0 1741 1766 1806 Size: 1741 25 40 Fragments arranged by size: 1741 40 25 PspOMI G'GGCC_C Cuts at: 0 404 605 1671 1806 Size: 404 201 1066 135 Fragments arranged by size: 1066 404 201 135 PvuII CAG'CTG Cuts at: 0 1504 1806 Size: 1504 302 SacII CC_GC'GG Cuts at: 0 1090 1806 Size: 1090 716 SapI GCTCTTCn'nnn_ Cuts at: 0 1495 1806 Size: 1495 311 SfcI C'TryA_G Cuts at: 0 930 1650 1806 Size: 930 720 156 Fragments arranged by size: 930 720 156 SmlI C'TyrA_G Cuts at: 0 1666 1806 Size: 1666 140 StyI C'CwwG_G Cuts at: 0 777 1260 1462 1622 1806 Size: 777 483 202 160 184 Fragments arranged by size: 777 483 202 184 160 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 865 872 1806 Size: 865 7 934 Fragments arranged by size: 934 865 7 TatI w'GTAC_w Cuts at: 0 281 701 1541 1568 1806 Size: 281 420 840 27 238 Fragments arranged by size: 840 420 281 238 27 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1307 1339 1806 Size: 1307 32 467 Fragments arranged by size: 1307 467 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 693 1044 1806 Size: 693 351 762 Fragments arranged by size: 762 693 351 Enzymes that do cut: AccI Acc65I AcuI AfeI AflIII AlwNI ApaI ApaLI ApoI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII Bme1580I BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI BsrFI BstAPI BstXI BstYI BtgI BtgZI BtsI EaeI EarI EciI Eco57MI EcoO109I EcoRI FalI HaeII Hin4I KpnI MluI MscI MslI MspA1I NaeI NcoI NgoMIV NspI PasI PciI PflMI PmlI PpuMI PspOMI PvuII SacII SapI SfcI SmlI StyI TaqII TatI TstI XcmI Enzymes that do not cut: AarI AatII AclI AflII AgeI AhdI AleI AlfI AloI AscI AseI AsiSI AvaI AvrII BbeI BbvCI BfrBI BlpI BmgBI BmrI BmtI BplI BsgI BsiEI BsiWI BspEI BspHI BsrDI BsrGI BssHII BssSI BstBI BstEII BstZ17I Bsu36I ClaI CspCI DraI DraIII DrdI EagI EcoICRI EcoNI EcoRV FseI FspI FspAI HincII HindIII HpaI KasI MfeI MmeI NarI NdeI NheI NotI NruI NsiI PacI PfoI PmeI PpiI PshAI PsiI PspXI PsrI PstI PvuI RsrII SacI SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI Tth111I XbaI XhoI XmaI XmnI ZraI