Restriction map for
pFN21AE4513
(Circular) (Six-base) MAPSORT of: pFN21AE4513.seq Check: 927 from: 1 to: 6331
pFN21AE4513 6331 bp
With 182 enzymes: SgfI *
March 28, 2012 14:38 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4141 278
Size: 53 83 186 936 2605 2468
Fragments arranged by size:
2605 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2304 2401 2636 3674 1937
Size: 367 97 235 1038 4594
Fragments arranged by size:
4594 1038 367 235 97
Acc65I G'GTAC_C
Cuts at: 3652 3652
Size: 6331
AclI AA'CG_TT
Cuts at: 1563 4458 4831 1563
Size: 2895 373 3063
Fragments arranged by size:
3063 2895 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2387 2494 2843 3265 4394 5778 6046 1632
Size: 755 107 349 422 1129 1384 268 1917
Fragments arranged by size:
1917 1384 1129 755 422 349 268 107
AflII C'TTAA_G
Cuts at: 820 1017 3133 820
Size: 197 2116 4018
Fragments arranged by size:
4018 2116 197
AflIII A'CryG_T
Cuts at: 1203 5230 6207 1203
Size: 4027 977 1327
Fragments arranged by size:
4027 1327 977
AgeI A'CCGG_T
Cuts at: 3014 5162 3014
Size: 2148 4183
AhdI GACnn_n'nnGTC
Cuts at: 2988 5060 2988
Size: 2072 4259
AleI CACnn'nnGTG
Cuts at: 1166 3209 1166
Size: 2043 4288
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2492 1184
Size: 1308 5023
AlwNI CAG_nnn'CTG
Cuts at: 2059 4074 5646 2059
Size: 2015 1572 2744
Fragments arranged by size:
2744 2015 1572
ApaI G_GGCC'C
Cuts at: 2201 2201
Size: 6331
ApaLI G'TGCA_C
Cuts at: 4388 5544 4388
Size: 1156 5175
ApoI r'AATT_y
Cuts at: 1493 3053 3640 3866 4018 6127 1493
Size: 1560 587 226 152 2109 1697
Fragments arranged by size:
2109 1697 1560 587 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4885 160
Size: 872 3853 1606
Fragments arranged by size:
3853 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6331
AvaI C'yCGr_G
Cuts at: 1870 1943 2645 3455 3656 1870
Size: 73 702 810 201 4545
Fragments arranged by size:
4545 810 702 201 73
BamHI G'GATC_C
Cuts at: 3591 3661 4028 3591
Size: 70 367 5894
Fragments arranged by size:
5894 367 70
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2220 2608 3652 5107 618
Size: 325 217 627 433 388 1044 1455 1842
Fragments arranged by size:
1842 1455 1044 627 433 388 325 217
BanII G_rGCy'C
Cuts at: 1391 2201 2418 2895 3626 3650 1391
Size: 810 217 477 731 24 4072
Fragments arranged by size:
4072 810 731 477 217 24
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2530 2740 2839 3525 928
Size: 971 631 210 99 686 3734
Fragments arranged by size:
3734 971 686 631 210 99
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4522 4556 1903
Size: 34 2585 34 3678
Fragments arranged by size:
3678 2585 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2955 4223 5433 2955
Size: 1268 1210 3853
Fragments arranged by size:
3853 1268 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6331
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2295 3197 4942
Size: 107 122 71 631 605 623 902 1745
Cuts at: 4942 136
Size: 1525
Fragments arranged by size:
1745 1525 902 631 623 605 122 107
71
BglII A'GATC_T
Cuts at: 2118 2916 6326 2118
Size: 798 3410 2123
Fragments arranged by size:
3410 2123 798
BlpI GC'TnA_GC
Cuts at: 2879 3748 2879
Size: 869 5462
Bme1580I G_kGCm'C
Cuts at: 1790 2201 3216 4392 5548 1790
Size: 411 1015 1176 1156 2573
Fragments arranged by size:
2573 1176 1156 1015 411
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6331
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2084 2374 2698
Size: 416 53 83 399 24 667 290 324
Cuts at: 2698 4062 5020 442
Size: 1364 958 1753
Fragments arranged by size:
1753 1364 958 667 416 399 324 290
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6331
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2894 3083 3612 2894
Size: 189 529 5613
Fragments arranged by size:
5613 529 189
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3062 3610 4991 1239
Size: 1823 548 1381 2579
Fragments arranged by size:
2579 1823 1381 548
Bpu10I CC'TnA_GC
Cuts at: 2579 2579
Size: 6331
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4456 5321 5619 4456
Size: 865 298 5168
Fragments arranged by size:
5168 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2421 3479 4994 882
Size: 617 922 1058 1515 2219
Fragments arranged by size:
2219 1515 1058 922 617
BsaAI yAC'GTr
Cuts at: 493 1204 4122 493
Size: 711 2918 2702
Fragments arranged by size:
2918 2702 711
BsaBI GATnn'nnATC
Cuts at: 3268 6325 3268
Size: 3057 3274
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4138 4520 5155 275
Size: 53 83 186 936 2605 382 635 1451
Fragments arranged by size:
2605 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2498 2958 3014 4764 5162 5436 5583 5994 2498
Size: 460 56 1750 398 274 147 411 2835
Fragments arranged by size:
2835 1750 460 411 398 274 147 56
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2064 2094 2153 2183 2403
Size: 30 165 30 661 30 59 30 220
Cuts at: 2403 2433 1178
Size: 30 5076
Fragments arranged by size:
5076 661 220 165 59 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2903 3443 1186
Size: 1717 540 4074
Fragments arranged by size:
4074 1717 540
BseYI C'CCAG_C
Cuts at: 2298 3106 5534 6030 2298
Size: 808 2428 496 2599
Fragments arranged by size:
2599 2428 808 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2523 1163
Size: 573 787 4971
Fragments arranged by size:
4971 787 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3781 4542 5570 1540
Size: 275 186 1780 761 1028 2301
Fragments arranged by size:
2301 1780 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2190 2585 2895 4392 4477 5548 2190
Size: 395 310 1497 85 1071 2973
Fragments arranged by size:
2973 1497 1071 395 310 85
BsmI GAATG_Cn'
Cuts at: 3842 3935 3842
Size: 93 6238
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2190 2201 2418 2585 2895 3216 3626
Size: 399 400 11 217 167 310 321 410
Cuts at: 3626 3650 4392 4477 5548 1391
Size: 24 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 410 400 399 321 310
217 167 85 24 11
BspEI T'CCGG_A
Cuts at: 2958 5994 2958
Size: 3036 3295
BspHI T'CATG_A
Cuts at: 4220 5950 4220
Size: 1730 4601
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2118 2387 2474 2484 2913 3686 844
Size: 1274 269 87 10 429 773 3489
Fragments arranged by size:
3489 1274 773 429 269 87 10
BsrBI CCG'CTC
Cuts at: 1394 1823 4218 1394
Size: 429 2395 3507
Fragments arranged by size:
3507 2395 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4826 5000 65
Size: 1183 3578 174 1396
Fragments arranged by size:
3578 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3014 3147 4975 5162 1700
Size: 110 1204 133 1828 187 2869
Fragments arranged by size:
2869 1828 1204 187 133 110
BsrGI T'GTAC_A
Cuts at: 96 2233 96
Size: 2137 4194
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6331
BssSI C'ACGA_G
Cuts at: 2417 4391 5403 2417
Size: 1974 1012 3345
Fragments arranged by size:
3345 1974 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6331
BstBI TT'CG_AA
Cuts at: 5135 5135
Size: 6331
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6331
BstXI CCAn_nnnn'nTGG
Cuts at: 2533 2533
Size: 6331
BstYI r'GATC_y
Cuts at: 1972 2019 2118 2916 3591 3661 4028 4413 4430
Size: 47 99 798 675 70 367 385 17
Cuts at: 4430 5882 5968 5980 6326 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 798 675 385 367 346 99
86 70 47 17 12
BstZ17I GTA'TAC
Cuts at: 2402 2402
Size: 6331
BtgI C'CryG_G
Cuts at: 513 1065 2193 2277 513
Size: 552 1128 84 4567
Fragments arranged by size:
4567 1128 552 84
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5592
Fragments arranged by size:
5592 683 56
BtsI GCAGTG_nn'
Cuts at: 3469 3847 4641 4661 3469
Size: 378 794 20 5139
Fragments arranged by size:
5139 794 378 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6331
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6296
DraI TTT'AAA
Cuts at: 3635 3635
Size: 6331
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2776 5338 809
Size: 507 1460 2562 1802
Fragments arranged by size:
2562 1802 1460 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2097 2446 2665
Size: 54 1425 50 275 18 267 349 219
Cuts at: 2665 2925 3778 4667 6296 8
Size: 260 853 889 1629 43
Fragments arranged by size:
1629 1425 889 853 349 275 267 260
219 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3778 1537
Size: 275 1966 4090
Fragments arranged by size:
4090 1966 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2859 3296 3479 4261 1353
Size: 1506 437 183 782 3423
Fragments arranged by size:
3423 1506 782 437 183
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2229 4918 5292 5438 387
Size: 1064 138 640 2689 374 146 1280
Fragments arranged by size:
2689 1280 1064 640 374 146 138
EcoICRI GAG'CTC
Cuts at: 2893 2893
Size: 6331
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2387 2494 2843 3062 3265 3610 4394
Size: 393 755 107 349 219 203 345 784
Cuts at: 4394 4991 5778 6046 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 787 784 755 597 393 349 345
268 219 203 107
EcoNI CCTnn'n_nnAGG
Cuts at: 2909 3586 2909
Size: 677 5654
EcoO109I rG'GnC_Cy
Cuts at: 2177 2818 2177
Size: 641 5690
EcoRI G'AATT_C
Cuts at: 3640 4018 3640
Size: 378 5953
FspI TGC'GCA
Cuts at: 4837 4837
Size: 6331
HaeII r_GCGC'y
Cuts at: 1678 5478 1678
Size: 3800 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2974 3006 3177 3209 3478
Size: 32 135 32 1402 32 171 32 269
Cuts at: 3478 3510 4972 5004 5046 5078 1373
Size: 32 1462 32 42 32 2626
Fragments arranged by size:
2626 1462 1402 269 171 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2637 2724 3064 3675 3921 669
Size: 988 281 699 87 340 611 246 3079
Fragments arranged by size:
3079 988 699 611 340 281 246 87
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6331
HpaI GTT'AAC
Cuts at: 2724 3921 2724
Size: 1197 5134
KpnI G_GTAC'C
Cuts at: 3656 3656
Size: 6331
MfeI C'AATT_G
Cuts at: 3930 3930
Size: 6331
MluI A'CGCG_T
Cuts at: 6207 6207
Size: 6331
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2382 5445 5629 1305
Size: 1077 3063 184 2007
Fragments arranged by size:
3063 2007 1077 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2099 2667 2927 10
Size: 54 1425 343 267 568 260 3414
Fragments arranged by size:
3414 1425 568 343 267 260 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2531 2764 3209 4289 4648 4807 518
Size: 648 1365 233 445 1080 359 159 2042
Fragments arranged by size:
2042 1365 1080 648 445 359 233 159
MspA1I CmG'CkG
Cuts at: 1606 2493 3167 3285 3388 3747 4424 5572 5817
Size: 887 674 118 103 359 677 1148 245
Cuts at: 5817 1606
Size: 2120
Fragments arranged by size:
2120 1148 887 677 674 359 245 118
103
NaeI GCC'GGC
Cuts at: 3149 3149
Size: 6331
NcoI C'CATG_G
Cuts at: 513 1065 2193 2277 513
Size: 552 1128 84 4567
Fragments arranged by size:
4567 1128 552 84
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6331
NgoMIV G'CCGG_C
Cuts at: 3147 3147
Size: 6331
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6331
NotI GC'GGCC_GC
Cuts at: 3778 3778
Size: 6331
NspI r_CATG'y
Cuts at: 1132 1229 2066 3689 5234 1132
Size: 97 837 1623 1545 2229
Fragments arranged by size:
2229 1623 1545 837 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6331
PciI A'CATG_T
Cuts at: 5230 5230
Size: 6331
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2787 1108
Size: 1679 4652
PfoI T'CCnGG_A
Cuts at: 2022 2022
Size: 6331
PmeI GTTT'AAAC
Cuts at: 3635 3635
Size: 6331
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4400 4432 5913 5945 4400
Size: 32 1481 32 4786
Fragments arranged by size:
4786 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6331
PsiI TTA'TAA
Cuts at: 3901 3901
Size: 6331
PspOMI G'GGCC_C
Cuts at: 2197 2197
Size: 6331
PstI C_TGCA'G
Cuts at: 830 2118 2471 2721 2910 3683 830
Size: 1288 353 250 189 773 3478
Fragments arranged by size:
3478 1288 773 353 250 189
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6331
PvuII CAG'CTG
Cuts at: 3285 3285
Size: 6331
SacI G_AGCT'C
Cuts at: 2895 2895
Size: 6331
SalI G'TCGA_C
Cuts at: 1936 2635 3673 1936
Size: 699 1038 4594
Fragments arranged by size:
4594 1038 699
SbfI CC_TGCA'GG
Cuts at: 3683 3683
Size: 6331
ScaI AGT'ACT
Cuts at: 4579 6291 4579
Size: 1712 4619
SfcI C'TryA_G
Cuts at: 826 1045 2114 2467 2717 2906 3028 3599 3679
Size: 219 1069 353 250 189 122 571 80
Cuts at: 3679 4814 5495 5686 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1135 1069 681 571 353 250 219
191 189 122 80
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6331
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6331
SmaI CCC'GGG
Cuts at: 1872 3658 1872
Size: 1786 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3133 4435 5336 5598 820
Size: 197 926 1190 1302 901 262 1553
Fragments arranged by size:
1553 1302 1190 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4122 493
Size: 3629 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6331
SphI G_CATG'C
Cuts at: 1132 3689 1132
Size: 2557 3774
SspI AAT'ATT
Cuts at: 5 52 4255 5
Size: 47 4203 2081
Fragments arranged by size:
4203 2081 47
StuI AGG'CCT
Cuts at: 1517 3581 3618 1517
Size: 2064 37 4230
Fragments arranged by size:
4230 2064 37
StyI C'CwwG_G
Cuts at: 513 1065 2180 2193 2277 2316 2922 2928 3216
Size: 552 1115 13 84 39 606 6 288
Cuts at: 3216 3770 513
Size: 554 3074
Fragments arranged by size:
3074 1115 606 554 552 288 84 39
13 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2173 2282 2596 3032 4358 4375 4528
Size: 1255 593 109 314 436 1326 17 153
Cuts at: 4528 4713 5158 325
Size: 185 445 1498
Fragments arranged by size:
1498 1326 1255 593 445 436 314 185
153 109 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2233 2937 4577
Size: 274 80 33 51 1443 256 704 1640
Cuts at: 4577 6289 96
Size: 1712 138
Fragments arranged by size:
1712 1640 1443 704 274 256 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2153 2185 2333 2365 2403 2435 3363 3395 6291
Size: 32 148 32 38 32 928 32 2896
Cuts at: 6291 6323 2153
Size: 32 2161
Fragments arranged by size:
2896 2161 928 148 38 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6331
XbaI T'CTAG_A
Cuts at: 3667 3667
Size: 6331
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2788 3086 2788
Size: 298 6033
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6331
XmaI C'CCGG_G
Cuts at: 1870 3656 1870
Size: 1786 4545
XmnI GAAnn'nnTTC
Cuts at: 4460 4460
Size: 6331
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4139 276
Size: 53 83 186 936 2605 2468
Fragments arranged by size:
2605 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BsiWI BsmBI Bsu36I DraIII EcoRV FalI FseI
FspAI KasI NarI NruI NsiI PacI PmlI PpuMI
PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI