Restriction map for
pFN21AE4560
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# NO CUT ENZYMES ( 120 enzymes) #
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AatII Acc65I AccIII AclI AflII
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaLI AscI Asp718I AspEI
AviII AvrII BanI BbeI BbrPI
BcgI BciVI BclI BfrI BglI
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaI BsaWI BseAI BsgI BsiEI
BsiWI BsmBI BspEI BspHI BspLU11I
BsrBI BsrFI BssHII BstAPI BstBI
Bsu36I CelII CfoI Cfr10I CpoI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoRI EcoRV
EcoT22I FbaI FseI FspI HaeII
HhaI HinP1I HindIII HpaI KasI
KpnI KspI MamI MluI MroI
MspA1I NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PpuMI PshAI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SfcI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI Sse8387I
StuI XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AcyI 181 GGCGTC G[GA]^CG[CT]C
AlwNI 1698 CAGGGGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 361 GGGCCC GGGCC^C
Asp700I 82 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 295 GTGCTC G[AT]GC[AT]^C
AvaI 254 CTCGGG C^[CT]CG[GA]G
BalI 231 TGGCCA TGG^CCA
BamHI 105 GGATCC G^GATCC
BbsI 82 GAAGAC GAAGAC(2/6)
BbvCI 1236 CCTCAGC CCTCAGC(-5/-2)
BpuAI 82 GAAGAC GAAGAC(2/6)
BsaHI 181 GGCGTC G[GA]^CG[CT]C
BseRI 1690 CTCCTC GAGGAG(10/8)
BsiHKAI 295 GTGCTC G[AT]GC[AT]^C
BsmAI 23 GAGAC GTCTC(1/5)
BsmI 1067 GAATGC GAATGC(1/-1)
BsoBI 254 CTCGGG C^[CT]CG[GA]G
Bsp1407I 1742 TGTACA T^GTACA
BspDI 625 ATCGAT AT^CGAT
BspMI 1059 GCAGGT ACCTGC(4/8)
BsrDI 321 CATTGC GCAATG(2/0)
BsrGI 1742 TGTACA T^GTACA
Bst1107I 2043 GTATAC GTA^TAC
BstEII 1577 GGTTACC G^GT[ACGT]ACC
BstPI 1577 GGTTACC G^GT[ACGT]ACC
BstZ17I 2043 GTATAC GTA^TAC
ClaI 625 ATCGAT AT^CGAT
DraII 360 GGGGCCC [GA]G^G[ACGT]CC[CT]
DraIII 1600 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1100 CCATGG C^C[GA][CT]GG
EarI 265 CTCTTC CTCTTC(1/4)
EcoO109I 360 GGGGCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 1577 GGTTACC G^GT[ACGT]ACC
HgaI 182 GCGTC GACGC(5/10)
Hin1I 181 GGCGTC G[GA]^CG[CT]C
HincII 1384 GTCAAC GT[CT]^[GA]AC
HindII 1384 GTCAAC GT[CT]^[GA]AC
Ksp632I 265 CTCTTC CTCTTC(1/4)
MaeII 1388 ACGT A^CGT
MfeI 670 CAATTG C^AATTG
MluNI 231 TGGCCA TGG^CCA
MscI 231 TGGCCA TGG^CCA
MunI 670 CAATTG C^AATTG
NcoI 1100 CCATGG C^CATGG
PflMI 755 CCAACAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SexAI 1573 ACCTGGT A^CC[AT]GGT
SspBI 1742 TGTACA T^GTACA
SspI 1462 AATATT AAT^ATT
SwaI 851 ATTTAAAT ATTT^AAAT
Van91I 755 CCAACAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 82 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 17 enzymes) #
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AccI 1667 GTCTAC GT^[AC][GT]AC
2043 GTATAC
AccII 303 CGCG CG^CG
1364 CGCG
AseI 618 ATTAAT AT^TAAT
1498 ATTAAT
AsnI 618 ATTAAT AT^TAAT
1498 ATTAAT
AspI 1012 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1075 GACATTGTC
BglII 374 AGATCT A^GATCT
420 AGATCT
BpmI 783 CTCCAG CTGGAG(16/14)
91 CTGGAG
BssSI 645 CACGAG CACGAG(-5/-1)
2049 CTCGTG
BstUI 303 CGCG CG^CG
1364 CGCG
EaeI 231 TGGCCA [CT]^GGCC[GA]
305 CGGCCA
HphI 1049 TCACC GGTGA(8/7)
1519 TCACC
MvnI 303 CGCG CG^CG
1364 CGCG
PflFI 1012 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1075 GACATTGTC
PshBI 618 ATTAAT AT^TAAT
1498 ATTAAT
SphI 1241 GCATGC GCATG^C
2014 GCATGC
Tsp45I 1018 GTCAC ^GT[GC]AC
1518 GTCAC
Tth111I 1012 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1075 GACATTGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AvaII BanII BbvI BcnI
BfaI BmyI BsaJI BsiYI BslI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI BstXI BstYI Cac8I Cfr13I
DdeI DpnII DraI EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HinfI HpaII ItaI MaeI MaeIII
MboI MboII MflI MnlI MseI
MslI MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
StyI TaqI TfiI Tru9I TseI
Tsp509I TspRI TthHB8I XcmI XhoII