(Circular) (Six-base) MAPSORT of: pFN21AE4560.seq Check: 7531 from: 1 to: 6760 pFN21AE4560 6760 bp With 182 enzymes: SgfI * March 28, 2012 14:15 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3055 3055 Size: 6760 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4570 278 Size: 53 83 186 936 3034 2468 Fragments arranged by size: 3034 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3673 4049 4103 1937 Size: 1736 376 54 4594 Fragments arranged by size: 4594 1736 376 54 Acc65I G'GTAC_C Cuts at: 4081 4081 Size: 6760 AclI AA'CG_TT Cuts at: 1563 4887 5260 1563 Size: 3324 373 3063 Fragments arranged by size: 3324 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2031 2156 3174 3585 4823 6207 6475 1632 Size: 399 125 1018 411 1238 1384 268 1917 Fragments arranged by size: 1917 1384 1238 1018 411 399 268 125 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6563 AflIII A'CryG_T Cuts at: 1203 5659 6636 1203 Size: 4456 977 1327 Fragments arranged by size: 4456 1327 977 AgeI A'CCGG_T Cuts at: 5591 5591 Size: 6760 AhdI GACnn_n'nnGTC Cuts at: 5489 5489 Size: 6760 AleI CACnn'nnGTG Cuts at: 1166 2652 3065 1166 Size: 1486 413 4861 Fragments arranged by size: 4861 1486 413 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6760 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2207 2239 2207 Size: 32 6728 AlwNI CAG_nnn'CTG Cuts at: 3708 4503 6075 3708 Size: 795 1572 4393 Fragments arranged by size: 4393 1572 795 ApaI G_GGCC'C Cuts at: 2370 2370 Size: 6760 ApaLI G'TGCA_C Cuts at: 4817 5973 4817 Size: 1156 5604 ApoI r'AATT_y Cuts at: 1493 2860 2898 2954 3796 4069 4295 4447 6556 Size: 1367 38 56 842 273 226 152 2109 Cuts at: 6556 1493 Size: 1697 Fragments arranged by size: 2109 1697 1367 842 273 226 152 56 38 AseI AT'TA_AT Cuts at: 160 1032 2624 3504 5314 160 Size: 872 1592 880 1810 1606 Fragments arranged by size: 1810 1606 1592 880 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6760 AvaI C'yCGr_G Cuts at: 1870 1943 2259 4085 1870 Size: 73 316 1826 4545 Fragments arranged by size: 4545 1826 316 73 BamHI G'GATC_C Cuts at: 2110 4090 4457 2110 Size: 1980 367 4413 Fragments arranged by size: 4413 1980 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4081 5536 618 Size: 325 217 627 2294 1455 1842 Fragments arranged by size: 2294 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2175 2370 3129 4079 1391 Size: 784 195 759 950 4072 Fragments arranged by size: 4072 950 784 759 195 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2094 928 Size: 971 195 5594 Fragments arranged by size: 5594 971 195 BbvCI CC'TCA_GC Cuts at: 3242 3242 Size: 6760 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4951 4985 1903 Size: 34 3014 34 3678 Fragments arranged by size: 3678 3014 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4652 5862 4652 Size: 1210 5550 BclI T'GATC_A Cuts at: 1548 1548 Size: 6760 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5371 136 Size: 107 122 71 631 605 3699 1525 Fragments arranged by size: 3699 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2379 2425 6755 2379 Size: 46 4330 2384 Fragments arranged by size: 4330 2384 46 BlpI GC'TnA_GC Cuts at: 4177 4177 Size: 6760 Bme1580I G_kGCm'C Cuts at: 1790 2370 4821 5977 1790 Size: 580 2451 1156 2573 Fragments arranged by size: 2573 2451 1156 580 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6760 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4491 5449 442 Size: 416 53 83 399 24 3074 958 1753 Fragments arranged by size: 3074 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6760 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3956 3956 Size: 6760 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2117 2773 5420 1239 Size: 878 656 2647 2579 Fragments arranged by size: 2647 2579 878 656 Bpu10I CC'TnA_GC Cuts at: 3242 3242 Size: 6760 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4885 5750 6048 4885 Size: 865 298 5597 Fragments arranged by size: 5597 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5423 882 Size: 617 3924 2219 Fragments arranged by size: 3924 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4551 493 Size: 711 3347 2702 Fragments arranged by size: 3347 2702 711 BsaBI GATnn'nnATC Cuts at: 6754 6754 Size: 6760 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2187 4567 4949 5584 Size: 53 83 186 936 654 2380 382 635 Cuts at: 5584 275 Size: 1451 Fragments arranged by size: 2380 1451 936 654 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5193 5591 5865 6012 6423 5193 Size: 398 274 147 411 5530 Fragments arranged by size: 5530 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2207 2237 1178 Size: 30 165 30 804 30 5701 Fragments arranged by size: 5701 804 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3686 1186 Size: 2500 4260 BseYI C'CCAG_C Cuts at: 5963 6459 5963 Size: 496 6264 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6187 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4210 4971 5999 1540 Size: 275 186 2209 761 1028 2301 Fragments arranged by size: 2301 2209 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2304 4821 4906 5977 2304 Size: 2517 85 1071 3087 Fragments arranged by size: 3087 2517 1071 85 BsmI GAATG_Cn' Cuts at: 3078 4271 4364 3078 Size: 1193 93 5474 Fragments arranged by size: 5474 1193 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2175 2304 2370 3129 4079 4821 4906 Size: 399 385 129 66 759 950 742 85 Cuts at: 4906 5977 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 950 759 742 399 385 129 85 66 BspEI T'CCGG_A Cuts at: 6423 6423 Size: 6760 BspHI T'CATG_A Cuts at: 4649 6379 4649 Size: 1730 5030 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3055 4115 844 Size: 2211 1060 3489 Fragments arranged by size: 3489 2211 1060 BsrBI CCG'CTC Cuts at: 1394 1823 4647 1394 Size: 429 2824 3507 Fragments arranged by size: 3507 2824 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2325 5255 5429 65 Size: 1183 1077 2930 174 1396 Fragments arranged by size: 2930 1396 1183 1077 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5404 5591 1700 Size: 110 3594 187 2869 Fragments arranged by size: 3594 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3747 96 Size: 3651 3109 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6760 BssSI C'ACGA_G Cuts at: 2650 4054 4820 5832 2650 Size: 1404 766 1012 3578 Fragments arranged by size: 3578 1404 1012 766 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6760 BstBI TT'CG_AA Cuts at: 5564 5564 Size: 6760 BstEII G'GTnAC_C Cuts at: 1184 3582 1184 Size: 2398 4362 BstXI CCAn_nnnn'nTGG Cuts at: 2234 2741 3067 3534 2234 Size: 507 326 467 5460 Fragments arranged by size: 5460 507 467 326 BstYI r'GATC_y Cuts at: 1972 2110 2265 2379 2425 2988 4090 4457 4842 Size: 138 155 114 46 563 1102 367 385 Cuts at: 4842 4859 6311 6397 6409 6755 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1102 563 385 367 346 155 138 114 86 46 17 12 BstZ17I GTA'TAC Cuts at: 4050 4050 Size: 6760 BtgI C'CryG_G Cuts at: 513 1065 2004 3105 513 Size: 552 939 1101 4168 Fragments arranged by size: 4168 1101 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2290 3959 487 Size: 683 56 1064 1669 3288 Fragments arranged by size: 3288 1669 1064 683 56 BtsI GCAGTG_nn' Cuts at: 3539 4276 5070 5090 3539 Size: 737 794 20 5209 Fragments arranged by size: 5209 794 737 20 ClaI AT'CG_AT Cuts at: 1554 2631 1554 Size: 1077 5683 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6725 DraI TTT'AAA Cuts at: 2469 2619 2859 4064 2469 Size: 150 240 1205 5165 Fragments arranged by size: 5165 1205 240 150 DraIII CAC_nnn'GTG Cuts at: 3610 3610 Size: 6760 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5767 809 Size: 507 4451 1802 Fragments arranged by size: 4451 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2236 2310 4207 Size: 54 1425 50 275 18 406 74 1897 Cuts at: 4207 5096 6725 8 Size: 889 1629 43 Fragments arranged by size: 1897 1629 1425 889 406 275 74 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4207 1537 Size: 275 2395 4090 Fragments arranged by size: 4090 2395 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2276 4690 1353 Size: 923 2414 3423 Fragments arranged by size: 3423 2414 923 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5347 5721 5867 387 Size: 1064 138 3758 374 146 1280 Fragments arranged by size: 3758 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2031 2117 2156 2773 3174 3585 4823 Size: 393 399 86 39 617 401 411 1238 Cuts at: 4823 5420 6207 6475 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1238 787 617 597 411 401 399 393 268 86 39 EcoO109I rG'GnC_Cy Cuts at: 2366 2366 Size: 6760 EcoRI G'AATT_C Cuts at: 4069 4447 4069 Size: 378 6382 FspI TGC'GCA Cuts at: 5266 5266 Size: 6760 HaeII r_GCGC'y Cuts at: 1678 5907 1678 Size: 4229 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2019 2051 2095 2127 2973 Size: 32 135 32 447 32 44 32 846 Cuts at: 2973 3005 5401 5433 5475 5507 1373 Size: 32 2396 32 42 32 2626 Fragments arranged by size: 2626 2396 846 447 135 44 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3391 4104 4350 669 Size: 988 281 1453 713 246 3079 Fragments arranged by size: 3079 1453 988 713 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6760 HpaI GTT'AAC Cuts at: 4350 4350 Size: 6760 KpnI G_GTAC'C Cuts at: 4085 4085 Size: 6760 MfeI C'AATT_G Cuts at: 2675 4359 2675 Size: 1684 5076 MluI A'CGCG_T Cuts at: 6636 6636 Size: 6760 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2663 5874 6058 1305 Size: 1358 3211 184 2007 Fragments arranged by size: 3211 2007 1358 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2238 10 Size: 54 1425 343 406 4532 Fragments arranged by size: 4532 1425 406 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2039 2652 2739 3065 3684 4718 5077 Size: 648 873 613 87 326 619 1034 359 Cuts at: 5077 5236 518 Size: 159 2042 Fragments arranged by size: 2042 1034 873 648 619 613 359 326 159 87 MspA1I CmG'CkG Cuts at: 1606 4176 4853 6001 6246 1606 Size: 2570 677 1148 245 2120 Fragments arranged by size: 2570 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 3105 513 Size: 552 939 1101 4168 Fragments arranged by size: 4168 1101 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6760 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6760 NotI GC'GGCC_GC Cuts at: 4207 4207 Size: 6760 NspI r_CATG'y Cuts at: 1132 1229 3250 3310 3406 4023 4118 5663 1132 Size: 97 2021 60 96 617 95 1545 2229 Fragments arranged by size: 2229 2021 1545 617 97 96 95 60 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6760 PciI A'CATG_T Cuts at: 5659 5659 Size: 6760 PflMI CCAn_nnn'nTGG Cuts at: 1108 2766 1108 Size: 1658 5102 PfoI T'CCnGG_A Cuts at: 2544 3012 3293 2544 Size: 468 281 6011 Fragments arranged by size: 6011 468 281 PmeI GTTT'AAAC Cuts at: 4064 4064 Size: 6760 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2207 2239 4829 4861 6342 6374 2207 Size: 32 2590 32 1481 32 2593 Fragments arranged by size: 2593 2590 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6760 PsiI TTA'TAA Cuts at: 2502 4330 2502 Size: 1828 4932 PspOMI G'GGCC_C Cuts at: 2366 2366 Size: 6760 PstI C_TGCA'G Cuts at: 830 4112 830 Size: 3282 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6760 SalI G'TCGA_C Cuts at: 1936 4102 1936 Size: 2166 4594 SbfI CC_TGCA'GG Cuts at: 4112 4112 Size: 6760 ScaI AGT'ACT Cuts at: 5008 6720 5008 Size: 1712 5048 SexAI A'CCwGG_T Cuts at: 3578 3578 Size: 6760 SfcI C'TryA_G Cuts at: 826 1045 4108 5243 5924 6115 826 Size: 219 3063 1135 681 191 1471 Fragments arranged by size: 3063 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6760 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6760 SmaI CCC'GGG Cuts at: 1872 4087 1872 Size: 2215 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4864 5765 6027 820 Size: 197 926 2921 901 262 1553 Fragments arranged by size: 2921 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4551 493 Size: 4058 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6760 SphI G_CATG'C Cuts at: 1132 3250 4023 4118 1132 Size: 2118 773 95 3774 Fragments arranged by size: 3774 2118 773 95 SspI AAT'ATT Cuts at: 5 52 3469 4684 5 Size: 47 3417 1215 2081 Fragments arranged by size: 3417 2081 1215 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6760 StyI C'CwwG_G Cuts at: 513 1065 2004 2158 2211 2387 3105 4199 513 Size: 552 939 154 53 176 718 1094 3074 Fragments arranged by size: 3074 1094 939 718 552 176 154 53 SwaI ATTT'AAAT Cuts at: 2859 2859 Size: 6760 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2773 4787 4804 4957 5142 5587 325 Size: 1255 1193 2014 17 153 185 445 1498 Fragments arranged by size: 2014 1498 1255 1193 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3189 3747 4033 Size: 274 80 33 51 1443 1212 558 286 Cuts at: 4033 5006 6718 96 Size: 973 1712 138 Fragments arranged by size: 1712 1443 1212 973 558 286 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3325 3357 6720 6752 3325 Size: 32 3363 32 3333 Fragments arranged by size: 3363 3333 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3020 3083 1316 Size: 1704 63 4993 Fragments arranged by size: 4993 1704 63 XbaI T'CTAG_A Cuts at: 4096 4096 Size: 6760 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2763 3064 3318 2763 Size: 301 254 6205 Fragments arranged by size: 6205 301 254 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6760 XmaI C'CCGG_G Cuts at: 1870 4085 1870 Size: 2215 4545 XmnI GAAnn'nnTTC Cuts at: 2091 4889 2091 Size: 2798 3962 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4568 276 Size: 53 83 186 936 3034 2468 Fragments arranged by size: 3034 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SfiI SfoI SrfI