(Circular) (Six-base) MAPSORT of: pFN21AE1333.seq Check: 1446 from: 1 to: 6496 pFN21AE1333 6496 bp With 182 enzymes: SgfI * August 16, 2010 14:53 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2252 2252 Size: 6496 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4306 278 Size: 53 83 186 936 2770 2468 Fragments arranged by size: 2770 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3839 1937 Size: 1902 4594 Acc65I G'GTAC_C Cuts at: 2991 3597 3817 2991 Size: 606 220 5670 Fragments arranged by size: 5670 606 220 AclI AA'CG_TT Cuts at: 1563 4623 4996 1563 Size: 3060 373 3063 Fragments arranged by size: 3063 3060 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4559 5943 6211 1632 Size: 2927 1384 268 1917 Fragments arranged by size: 2927 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6299 AflIII A'CryG_T Cuts at: 1203 5395 6372 1203 Size: 4192 977 1327 Fragments arranged by size: 4192 1327 977 AgeI A'CCGG_T Cuts at: 5327 5327 Size: 6496 AhdI GACnn_n'nnGTC Cuts at: 5225 5225 Size: 6496 AleI CACnn'nnGTG Cuts at: 1166 2565 2792 1166 Size: 1399 227 4870 Fragments arranged by size: 4870 1399 227 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6496 AlwNI CAG_nnn'CTG Cuts at: 3594 4239 5811 3594 Size: 645 1572 4279 Fragments arranged by size: 4279 1572 645 ApaLI G'TGCA_C Cuts at: 4553 5709 4553 Size: 1156 5340 ApoI r'AATT_y Cuts at: 1493 2414 2575 3055 3805 4031 4183 6292 1493 Size: 921 161 480 750 226 152 2109 1697 Fragments arranged by size: 2109 1697 921 750 480 226 161 152 AseI AT'TA_AT Cuts at: 160 1032 2904 5050 160 Size: 872 1872 2146 1606 Fragments arranged by size: 2146 1872 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6496 AvaI C'yCGr_G Cuts at: 1870 1943 3821 1870 Size: 73 1878 4545 Fragments arranged by size: 4545 1878 73 AvrII C'CTAG_G Cuts at: 2945 2945 Size: 6496 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2983 3016 2983 Size: 33 6463 BamHI G'GATC_C Cuts at: 3826 4193 3826 Size: 367 6129 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2991 3597 3607 3817 5272 Size: 325 217 627 1204 606 10 210 1455 Cuts at: 5272 618 Size: 1842 Fragments arranged by size: 1842 1455 1204 627 606 325 217 210 10 BanII G_rGCy'C Cuts at: 1391 2035 2892 3815 1391 Size: 644 857 923 4072 Fragments arranged by size: 4072 923 857 644 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5525 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4687 4721 1903 Size: 34 50 34 2666 34 3678 Fragments arranged by size: 3678 2666 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4388 5598 4388 Size: 1210 5286 BclI T'GATC_A Cuts at: 1548 1548 Size: 6496 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2253 5107 136 Size: 107 122 71 631 605 581 2854 1525 Fragments arranged by size: 2854 1525 631 605 581 122 107 71 BglII A'GATC_T Cuts at: 6491 6491 Size: 6496 BlpI GC'TnA_GC Cuts at: 3913 3913 Size: 6496 Bme1580I G_kGCm'C Cuts at: 1790 3612 4557 5713 1790 Size: 1822 945 1156 2573 Fragments arranged by size: 2573 1822 1156 945 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6496 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4227 5185 442 Size: 416 53 83 399 24 2810 958 1753 Fragments arranged by size: 2810 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6496 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2517 2517 Size: 6496 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2056 2512 2523 3414 3731 5156 1239 Size: 817 456 11 891 317 1425 2579 Fragments arranged by size: 2579 1425 891 817 456 317 11 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4621 5486 5784 4621 Size: 865 298 5333 Fragments arranged by size: 5333 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2680 2758 3200 3620 5159 882 Size: 617 1181 78 442 420 1539 2219 Fragments arranged by size: 2219 1539 1181 617 442 420 78 BsaAI yAC'GTr Cuts at: 493 1204 2363 3246 4287 493 Size: 711 1159 883 1041 2702 Fragments arranged by size: 2702 1159 1041 883 711 BsaBI GATnn'nnATC Cuts at: 3498 6490 3498 Size: 2992 3504 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4303 4685 5320 275 Size: 53 83 186 936 2770 382 635 1451 Fragments arranged by size: 2770 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4929 5327 5601 5748 6159 4929 Size: 398 274 147 411 5266 Fragments arranged by size: 5266 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6271 Fragments arranged by size: 6271 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2496 2526 2865 1186 Size: 1310 30 339 4817 Fragments arranged by size: 4817 1310 339 30 BseYI C'CCAG_C Cuts at: 5699 6195 5699 Size: 496 6000 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5923 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3946 4707 5735 1540 Size: 275 186 1945 761 1028 2301 Fragments arranged by size: 2301 1945 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3654 4557 4642 5713 3654 Size: 903 85 1071 4437 Fragments arranged by size: 4437 1071 903 85 BsmI GAATG_Cn' Cuts at: 4007 4100 4007 Size: 93 6403 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2035 2892 3612 3654 3815 4557 4642 Size: 399 245 857 720 42 161 742 85 Cuts at: 4642 5713 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 857 742 720 399 245 161 85 42 BspEI T'CCGG_A Cuts at: 6159 6159 Size: 6496 BspHI T'CATG_A Cuts at: 3202 4385 6115 3202 Size: 1183 1730 3583 Fragments arranged by size: 3583 1730 1183 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2252 3851 844 Size: 1408 1599 3489 Fragments arranged by size: 3489 1599 1408 BsrBI CCG'CTC Cuts at: 1394 1823 4383 1394 Size: 429 2560 3507 Fragments arranged by size: 3507 2560 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4991 5165 65 Size: 1183 3743 174 1396 Fragments arranged by size: 3743 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5140 5327 1700 Size: 110 3330 187 2869 Fragments arranged by size: 3330 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6496 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6496 BssSI C'ACGA_G Cuts at: 4556 5568 4556 Size: 1012 5484 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6496 BstBI TT'CG_AA Cuts at: 5300 5300 Size: 6496 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6496 BstXI CCAn_nnnn'nTGG Cuts at: 3239 3239 Size: 6496 BstYI r'GATC_y Cuts at: 1972 2264 3826 4193 4578 4595 6047 6133 6145 Size: 292 1562 367 385 17 1452 86 12 Cuts at: 6145 6491 1972 Size: 346 1977 Fragments arranged by size: 1977 1562 1452 385 367 346 292 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 3304 513 Size: 552 2239 3705 Fragments arranged by size: 3705 2239 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5757 Fragments arranged by size: 5757 683 56 BtsI GCAGTG_nn' Cuts at: 2839 3680 4012 4806 4826 2839 Size: 841 332 794 20 4509 Fragments arranged by size: 4509 841 794 332 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6496 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6461 DraI TTT'AAA Cuts at: 3800 3800 Size: 6496 DraIII CAC_nnn'GTG Cuts at: 2513 2513 Size: 6496 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5503 809 Size: 507 4187 1802 Fragments arranged by size: 4187 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3943 4832 6461 Size: 54 1425 50 275 18 2113 889 1629 Cuts at: 6461 8 Size: 43 Fragments arranged by size: 2113 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3943 1537 Size: 275 2131 4090 Fragments arranged by size: 4090 2131 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2040 2108 3342 4426 1353 Size: 687 68 1234 1084 3423 Fragments arranged by size: 3423 1234 1084 687 68 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5083 5457 5603 387 Size: 1064 138 3494 374 146 1280 Fragments arranged by size: 3494 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2056 2512 2523 3414 3731 4559 5156 Size: 393 424 456 11 891 317 828 597 Cuts at: 5156 5943 6211 1239 Size: 787 268 1524 Fragments arranged by size: 1524 891 828 787 597 456 424 393 317 268 11 EcoNI CCTnn'n_nnAGG Cuts at: 2071 2071 Size: 6496 EcoRI G'AATT_C Cuts at: 2414 3805 4183 2414 Size: 1391 378 4727 Fragments arranged by size: 4727 1391 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3591 3591 Size: 6496 FspI TGC'GCA Cuts at: 5002 5002 Size: 6496 HaeII r_GCGC'y Cuts at: 1678 5643 1678 Size: 3965 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2110 2142 5137 5169 5211 Size: 32 135 32 538 32 2995 32 42 Cuts at: 5211 5243 1373 Size: 32 2626 Fragments arranged by size: 2995 2626 538 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2202 3840 4086 669 Size: 988 281 264 1638 246 3079 Fragments arranged by size: 3079 1638 988 281 264 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6496 HpaI GTT'AAC Cuts at: 4086 4086 Size: 6496 KpnI G_GTAC'C Cuts at: 2995 3601 3821 2995 Size: 606 220 5670 Fragments arranged by size: 5670 606 220 MfeI C'AATT_G Cuts at: 2149 4095 2149 Size: 1946 4550 MluI A'CGCG_T Cuts at: 6372 6372 Size: 6496 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3367 3437 5610 5794 1305 Size: 2062 70 2173 184 2007 Fragments arranged by size: 2173 2062 2007 184 70 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4674 Fragments arranged by size: 4674 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2565 2792 2847 3196 3265 4454 4813 Size: 648 1399 227 55 349 69 1189 359 Cuts at: 4813 4972 518 Size: 159 2042 Fragments arranged by size: 2042 1399 1189 648 359 349 227 159 69 55 MspA1I CmG'CkG Cuts at: 1606 2421 2565 2610 2997 3912 4589 5737 5982 Size: 815 144 45 387 915 677 1148 245 Cuts at: 5982 1606 Size: 2120 Fragments arranged by size: 2120 1148 915 815 677 387 245 144 45 NcoI C'CATG_G Cuts at: 513 1065 3304 513 Size: 552 2239 3705 Fragments arranged by size: 3705 2239 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6496 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6496 NotI GC'GGCC_GC Cuts at: 3943 3943 Size: 6496 NspI r_CATG'y Cuts at: 1132 1229 2927 3854 5399 1132 Size: 97 1698 927 1545 2229 Fragments arranged by size: 2229 1698 1545 927 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6496 PciI A'CATG_T Cuts at: 5395 5395 Size: 6496 PflMI CCAn_nnn'nTGG Cuts at: 1108 2079 3269 1108 Size: 971 1190 4335 Fragments arranged by size: 4335 1190 971 PmeI GTTT'AAAC Cuts at: 3800 3800 Size: 6496 PmlI CAC'GTG Cuts at: 3246 3246 Size: 6496 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3636 3668 4565 4597 6078 6110 3636 Size: 32 897 32 1481 32 4022 Fragments arranged by size: 4022 1481 897 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6496 PsiI TTA'TAA Cuts at: 3315 4066 3315 Size: 751 5745 PstI C_TGCA'G Cuts at: 830 3410 3848 830 Size: 2580 438 3478 Fragments arranged by size: 3478 2580 438 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6496 PvuII CAG'CTG Cuts at: 2421 2610 2421 Size: 189 6307 SalI G'TCGA_C Cuts at: 1936 3838 1936 Size: 1902 4594 SapI GCTCTTCn'nnn_ Cuts at: 2040 2040 Size: 6496 SbfI CC_TGCA'GG Cuts at: 3848 3848 Size: 6496 ScaI AGT'ACT Cuts at: 4744 6456 4744 Size: 1712 4784 SexAI A'CCwGG_T Cuts at: 3593 3593 Size: 6496 SfcI C'TryA_G Cuts at: 826 1045 2605 3021 3406 3752 3844 4979 5660 Size: 219 1560 416 385 346 92 1135 681 Cuts at: 5660 5851 826 Size: 191 1471 Fragments arranged by size: 1560 1471 1135 681 416 385 346 219 191 92 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6496 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6496 SmaI CCC'GGG Cuts at: 1872 3823 1872 Size: 1951 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4600 5501 5763 820 Size: 197 926 2657 901 262 1553 Fragments arranged by size: 2657 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2363 4287 493 Size: 1870 1924 2702 Fragments arranged by size: 2702 1924 1870 SpeI A'CTAG_T Cuts at: 152 2456 3793 152 Size: 2304 1337 2855 Fragments arranged by size: 2855 2304 1337 SphI G_CATG'C Cuts at: 1132 3854 1132 Size: 2722 3774 SspI AAT'ATT Cuts at: 5 52 4420 5 Size: 47 4368 2081 Fragments arranged by size: 4368 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6496 StyI C'CwwG_G Cuts at: 513 1065 2945 3076 3232 3304 3935 513 Size: 552 1880 131 156 72 631 3074 Fragments arranged by size: 3074 1880 631 552 156 131 72 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2179 2299 2364 4523 4540 4693 4878 Size: 1255 599 120 65 2159 17 153 185 Cuts at: 4878 5323 325 Size: 445 1498 Fragments arranged by size: 2159 1498 1255 599 445 185 153 120 65 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4742 6454 96 Size: 274 80 33 51 1443 2765 1712 138 Fragments arranged by size: 2765 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3235 3267 6456 6488 3235 Size: 32 3189 32 3243 Fragments arranged by size: 3243 3189 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6496 XbaI T'CTAG_A Cuts at: 3832 3832 Size: 6496 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2928 3539 2928 Size: 611 5885 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6496 XmaI C'CCGG_G Cuts at: 1870 3821 1870 Size: 1951 4545 XmnI GAAnn'nnTTC Cuts at: 4625 4625 Size: 6496 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4304 276 Size: 53 83 186 936 2770 2468 Fragments arranged by size: 2770 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI