################################## # NO CUT ENZYMES ( 172 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcyI AflII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AvrII BalI BamHI BanI BbeI BbrPI BbsI BbvCI BbvI BcgI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmFI BsmI BsoBI BspDI BspEI BspHI BsrBI BsrFI BssHII BssSI Bst1107I BstBI BstUI BstXI BstZ17I Bsu36I Cac8I CelII Cfr10I ClaI CpoI DraI DraIII DrdI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoT22I FauI FbaI Fnu4HI FseI HaeII HapII HgaI Hin1I HincII HindII HpaI HpaII ItaI KasI KpnI Ksp632I KspI MaeI MamI MfeI MluI MluNI MroI MscI MspA1I MspI MunI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI StuI SwaI TseI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AflIII 571 ACATGT A^C[GA][CT]GT AluI 683 AGCT AG^CT AlwI 613 GATCC GGATC(4/5) ApaI 448 GGGCCC GGGCC^C AviII 214 TGCGCA TGC^GCA BanII 448 GGGCCC G[GA]GC[CT]^C BciVI 349 GGATAC GTATCC(6/5) BmyI 448 GGGCCC G[AGT]GC[ACT]^C BpmI 506 CTCCAG CTGGAG(16/14) BsaJI 87 CCATGG C^C[ACGT][ACGT]GG BseRI 407 CTCCTC GAGGAG(10/8) Bsp1286I 448 GGGCCC G[AGT]GC[ACT]^C Bsp1407I 327 TGTACA T^GTACA BspLU11I 571 ACATGT A^CATGT BspMI 419 GCAGGT ACCTGC(4/8) BsrGI 327 TGTACA T^GTACA BstAPI 206 GCATAACGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 230 GGTCACC G^GT[ACGT]ACC BstPI 230 GGTCACC G^GT[ACGT]ACC BstYI 612 AGATCC [GA]^GATC[CT] CfoI 215 GCGC GCG^C DpnII 613 GATC ^GATC DraII 448 GGGCCCC [GA]G^G[ACGT]CC[CT] DsaI 87 CCATGG C^C[GA][CT]GG EcoO109I 448 GGGCCCC [GA]G^G[ACGT]CC[CT] EcoO65I 230 GGTCACC G^GT[ACGT]ACC EcoRI 92 GAATTC G^AATTC EcoT14I 87 CCATGG C^C[AT][AT]GG FspI 214 TGCGCA TGC^GCA HaeIII 449 GGCC GG^CC HhaI 215 GCGC GCG^C HinP1I 215 GCGC G^CGC HindIII 682 AAGCTT A^AGCTT MaeII 211 ACGT A^CGT MboI 613 GATC ^GATC MflI 612 AGATCC [GA]^GATC[CT] MseI 661 TTAA T^TAA MwoI 206 GCATAACGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NcoI 87 CCATGG C^CATGG NdeII 613 GATC ^GATC NspI 571 ACATGT [GA]CATG^[CT] PleI 201 GACTC GAGTC(4/5) PshAI 584 GACAATGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 434 CTGCAG CTGCA^G Sau3AI 613 GATC ^GATC SmlI 550 CTTGAG C^T[CT][GA]AG SspBI 327 TGTACA T^GTACA StyI 87 CCATGG C^C[AT][AT]GG TaqI 759 TCGA T^CGA TfiI 292 GAATC G^A[AT]TC Tru9I 661 TTAA T^TAA TthHB8I 759 TCGA T^CGA XhoII 612 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AcsI 92 GAATTC [GA]^AATT[CT] 712 GAATTT AfaI 328 GTAC GT^AC 525 GTAC ApoI 92 GAATTC [GA]^AATT[CT] 712 GAATTT AvaII 401 GGTCC G^G[AT]CC 422 GGTCC BsiYI 41 CCTCTGTTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 413 CCAAATGCAGG BslI 41 CCTCTGTTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 413 CCAAATGCAGG BsrDI 58 GCAATG GCAATG(2/0) 763 GCAATG BssKI 9 CCTGG ^CC[ACGT]GG 508 CCAGG BstF5I 736 CATCC GGATG(2/0) 226 GGATG BstNI 9 CCTGG CC^[AT]GG 508 CCAGG DdeI 250 CTCAG C^T[ACGT]AG 609 CTTAG EcoRII 9 CCTGG ^CC[AT]GG 508 CCAGG EcoRV 36 GATATC GAT^ATC 731 GATATC FokI 736 CATCC GGATG(9/13) 226 GGATG HinfI 201 GACTC G^A[ACGT]TC 292 GAATC MslI 239 CACCAAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 599 CACCAAAATG MvaI 9 CCTGG CC^[AT]GG 508 CCAGG RsaI 328 GTAC GT^AC 525 GTAC ScrFI 9 CCTGG CC^[ACGT]GG 508 CCAGG SfcI 137 CTACAG C^T[GA][CT]AG 434 CTGCAG Tsp45I 231 GTCAC ^GT[GC]AC 701 GTGAC TspRI 15 CAGTG CA[GC]TG[ACGT][ACGT]^ 529 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 11 enzymes) # ######################################### BsmAI BsrI Cfr13I HphI MaeIII MboII MnlI NlaIII NlaIV Sau96I Tsp509I