################################## # NO CUT ENZYMES ( 149 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AcyI AflII AflIII AgeI Alw44I AocI Aor51HI ApaI ApaLI AscI Asp718I AspHI AspI AvrII BalI BamHI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfaI BfrI BglI BlnI BlpI Bpu1102I BpuAI BsaAI BsaHI BsaI BseAI BsgI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstUI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraIII DrdI DsaI EaeI EagI EarI EclXI Eco47III Eco52I Eco81I EcoRV EcoT14I EcoT22I FbaI FseI HaeII Hin1I HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MaeI MfeI MluI MluNI MroI MscI MslI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PvuI RcaI RsrII SacI SacII SalI SapI SbfI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI StyI SwaI TaqI Tth111I TthHB8I Van91I XbaI XcmI XhoI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AciI 736 GCGG CCGC(-3/-1) AclI 939 AACGTT AA^CGTT AcsI 678 GAATTC [GA]^AATT[CT] AhdI 962 GACCCTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC ApoI 678 GAATTC [GA]^AATT[CT] AseI 135 ATTAAT AT^TAAT AsnI 135 ATTAAT AT^TAAT Asp700I 597 GAAGTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 962 GACCCTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AviII 27 TGCGCA TGC^GCA BanI 205 GGCACC G^G[CT][GA]CC BanII 853 GAGCCC G[GA]GC[CT]^C BglII 413 AGATCT A^GATCT BpmI 520 CTCCAG CTGGAG(16/14) BsaBI 408 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 88 TCCGGT [AT]^CCGG[AT] BseRI 77 CTCCTC GAGGAG(10/8) BstEII 696 GGTGACC G^GT[ACGT]ACC BstPI 696 GGTGACC G^GT[ACGT]ACC BstXI 637 CCAAAGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CfoI 28 GCGC GCG^C DraI 498 TTTAAA TTT^AAA DraII 17 AGGCCCC [GA]G^G[ACGT]CC[CT] Eam1105I 962 GACCCTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 171 CCTGCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 17 AGGCCCC [GA]G^G[ACGT]CC[CT] EcoO65I 696 GGTGACC G^GT[ACGT]ACC EcoRI 678 GAATTC G^AATTC FauI 736 GCGGG CCCGC(4/6) FspI 27 TGCGCA TGC^GCA HgaI 147 GACGC GACGC(5/10) HhaI 28 GCGC GCG^C HinP1I 28 GCGC G^CGC MaeII 940 ACGT A^CGT MamI 408 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MboII 433 GAAGA GAAGA(8/7) PleI 743 GACTC GAGTC(4/5) PshBI 135 ATTAAT AT^TAAT Psp1406I 939 AACGTT AA^CGTT PstI 175 CTGCAG CTGCA^G PvuII 311 CAGCTG CAG^CTG ScaI 599 AGTACT AGT^ACT SexAI 643 ACCTGGT A^CC[AT]GGT SmaI 105 CCCGGG CCC^GGG SmlI 587 CTCAAG C^T[CT][GA]AG StuI 911 AGGCCT AGG^CCT XmaCI 105 CCCGGG C^CCGGG XmaI 105 CCCGGG C^CCGGG XmnI 597 GAAGTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AfaI 443 GTAC GT^AC 600 GTAC AluI 312 AGCT AG^CT 529 AGCT AlwNI 235 CAGTTGCTG CAG[ACGT][ACGT][ACGT]^CTG 364 CAGTTCCTG AvaI 68 CCCGAG C^[CT]CG[GA]G 105 CCCGGG BsoBI 68 CCCGAG C^[CT]CG[GA]G 105 CCCGGG BstF5I 776 GGATG GGATG(2/0) 801 GGATG Cac8I 177 GCAGGC GC[ACGT]^[ACGT]GC 313 GCTGGC DdeI 362 CTCAG C^T[ACGT]AG 702 CTCAG FokI 776 GGATG GGATG(9/13) 801 GGATG MspA1I 311 CAGCTG C[AC]G^C[GT]G 734 CAGCGG MwoI 19 GCCCCTGATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 66 GCCCCGAGAGC NlaIII 303 CATG CATG^ 929 CATG PshAI 147 GACGCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 225 GACCAATGTC RsaI 443 GTAC GT^AC 600 GTAC SfaNI 25 GATGC GCATC(5/9) 922 GATGC SfcI 175 CTGCAG C^T[GA][CT]AG 574 CTACAG TfiI 274 GATTC G^A[AT]TC 843 GATTC Tsp509I 282 AATT ^AATT 679 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 42 enzymes) # ######################################### AlwI AvaII BbvI BcnI BmyI BsaJI BsiYI BslI BsmFI Bsp1286I BsrI BssKI BstNI BstYI Cfr13I DpnII EcoRII Fnu4HI HaeIII HapII HinfI HpaII HphI ItaI MaeIII MboI MflI MnlI MseI MspI MvaI NciI NdeII NlaIV Sau3AI Sau96I ScrFI Tru9I TseI Tsp45I TspRI XhoII